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| 1 | HASTY modulates miRNA biogenesis by linking pri-miRNA transcription and processing显示文摘Post-transcriptional gene silencing mediated by microRNAs(miRNAs)modulates numerous developmental and stress response pathways.For the last two decades,HASTY(HST),the ortholog of human EXPORTIN 5,was considered to be a candidate protein that exports plant miRNAs from the nucleus to the cytoplasm.Here,we report that HST functions in the miRNA pathway independent of its cargo-exporting activity in Arabidopsis.We found that Arabidopsis mutants with impaired HST shuttling exhibit normal subcellular distribution of miRNAs.Interestingly,protein-protein interaction and microscopy assays showed that HST directly interacts with the microprocessor core component DCL1 through its N-terminal domain.Moreover,mass spectrometry analysis revealed that HST also interacts independently of its N-terminal domain with the mediator complex subunit MED37.Further experiments revealed that HST could act as a scaffold to facilitate the recruitment of DCL1 to genomic MIRNA loci by stabilizing the DCL1-MED37 complex,which in turn promotes the transcription and proper processing of primary miRNA transcripts(primiRNAs).Taken together,these results suggest that HST is likely associated with the formation of the miRNA biogenesis complex at MIRNA genes,promoting the transcription and processing of primiRNAs rather than the direct export of processed miRNAs from the nucleus. | Damian A.Cambiagno Axel J.Giudicatti Agustin L.Arce Delfina Gagliardi Lei Li Wei Yuan Derek S.Lundberg Detlef Weigel Pablo A.Manavella | 2021 | Molecular Plant2021，14，3： | 5 |
| 2 | Dissection of miRNA Pathways Using Arabidopsis Mesophyll Protoplasts显示文摘主要, post-transcriptionally,由指导,抄本劈开或互补 mRNA 的翻译压抑指向的 MicroRNAs (miRNAs ) 控制基因表示,从而调整的发展进程和压力回答。尽管有这块地的显著扩大,位于 miRNA 活动下面的机制充分没被理解。在这篇文章,我们在 Arabidopsis 叶肉原物描述一个短暂表达式系统,它为 miRNA 小径的解剖是高度顺从的。我们显示出那由短暂地主要 miRNAs 和目标模仿的 overexpressing,我们能操作 miRNA 层次并且因而影响他们的目标。而且,我们为确定开发了一套基于酶的传感器 miRNA 活动对明确地作出回应内长并且 overexpressed miRNAs 和目标模仿。我们证明这些 miRNA 传感器能被用来在特定的变化的 miRNA 活动,以及影响测试 miRNA 小径的通常认为的部件的影响,由 overexpression 或从相应异种的原物的使用。我们进一步证明我们的 miRNA 传感器能被用于在 miRNA 活动调查化学药品的效果。我们的基于房间的短暂表示系统快、容易建立,并且产生量的结果,是为 assaying miRNA 活动在的一个强大的工具 ? vivo。 | Claudia Martinho Ana Confraria Carlos Alexandre Elias Pierre Crozet Ignacio Rubio-Somoza Detlef Weigel Elena Baena-Gonzalez | 2015 | Molecular Plant2015，8，2： | 3 |
| 3 | Requirement of Homeobox Gene STIMPY / WOX9 for Arabidopsis Meristem Growth and Maintenance显示文摘 | Xuelin Wu Tsegaye Dabi Detlef Weigel | 2005 | Current Biology2005，，5： | 2 |
| 4 | miR156-Regulated SPL Transcription Factors Define an Endogenous Flowering Pathway in Arabidopsis thaliana显示文摘 | Jia-Wei Wang Benjamin Czech Detlef Weigel | 2009 | Cell2009，，4： | 1 |
| 5 | The Sequential Action of miR156 and miR172 Regulates Developmental Timing in Arabidopsis显示文摘 | Gang Wu Mee Yeon Park Susan R. Conway Jia-Wei Wang Detlef Weigel R. Scott Poethig | 2009 | Cell2009，，4： | 1 |
| 6 | Transcriptional Control of Gene Expression by MicroRNAs显示文摘 | Basel Khraiwesh M. Asif Arif Gotelinde I. Seumel Stephan Ossowski Detlef Weigel Ralf Reski Wolfgang Frank | 2010 | Cell2010，，1： | 1 |
| 7 | A Molecular Link between Stem Cell Regulation and Floral Patterning in Arabidopsis显示文摘 | Jan U. Lohmann Ray L. Hong Martin Hobe Maximilian A. Busch Fran?ois Parcy Rüdiger Simon Detlef Weigel | | Cell0，，： | 1 |
| 8 | Transformation of rice with the Arabidopsis floral regulator LEAFY causes early heading显示文摘 | Zuhua He Qun Zhu Tsegaye Dabi Debao Li Detlef Weigel Chris Lamb | 2000 | Transgenic Research2000，，3： | 1 |
| 9 | Transcriptional Control of Gene Expression by MicroRNAs显示文摘 | Basel Khraiwesh M. Asif Arif Gotelinde I. Seumel Stephan Ossowski Detlef Weigel Ralf Reski Wolfgang Frank | 2010 | Cell2010，，1： | 1 |
| 10 | Transformation of rice with the Arabidopsis floral regulator LEAFY causes early heading显示文摘 | Zuhua He Qun Zhu Tsegaye Dabi Debao Li Detlef Weigel Chris Lamb | 2000 | Transgenic Research2000，，3： | 1 |
| 11 | A developmental switch sufficient for flower initiation in diverse plants显示文摘 | Weigel Detlef Nilsson Ove | | 0，，： | 1 |
| 12 | The Sequential Action of miR156 and miR172 Regulates Developmental Timing in Arabidopsis显示文摘 | Gang Wu Mee Yeon Park Susan R. Conway Jia-Wei Wang Detlef Weigel R. Scott Poethig | 2009 | Cell2009，，4： | 1 |
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