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| 1 | Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells显示文摘人的导致的 pluripotent 茎(iPS ) 房间类似于胚胎的茎(ES ) 房间,和罐头强烈地增殖并且区分进许多房间类型。然而,人的 iPS 房间的肝的区别还没被报导了。在这份报告,人的 iPS 房间被导致由一个逐步的协议区分进肝的房间。肝房间标记的表达式和人的 iPS 的肝相关的函数导出房间的房间被监视并且与区分的人的 ES 房间和主要人的 hepatocytes 的相比。在白天 7 点的约 60% 区分的人的 iPS 房间表示了肝的标记 alpha fetoprotein 和白长袍的。在白天 21 点的区分的房间包括白朊 Asecretion,肝糖合成,脲生产和可诱导的细胞色素 P450 活动展出了肝房间功能。肝的标记的表示和 iPS 的肝相关的功能导出房间的肝的房间比得上人的 ES 导出房间的肝的房间的。这些结果证明人的 iPS 房间,类似于人的 ES 房间,能高效地被导致区分房间进象 hepatocyte 一样。 | Zhihua Song Jun Cai Yanxia Liu Dongxin Zhao Jun Yong Shuguang Duo Xijun Song Yushan Guo Yang Zhao Han Qin Xiaolei Yin Chen Wu Jie Che Shichun Lu Mingxiao Ding Hongkui Deng | 2009 | Cell Research2009，19，11： | 63 |
| 2 | XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice显示文摘 | Chunlian Wang Xiaoping Zhang Yinglun Fan Ying Gao Qinlong Zhu Chongke Zheng Tengfei Qin Yanqiang Li Jinying Che Mingwei Zhang Bing Yang Yaoguang Liu Kaijun Zhao | 2015 | Molecular Plant2015，8，2： | 55 |
| 3 | Danshen: a phytochemical and pharmacological overview显示文摘Danshen, the dried root or rhizome of Salvia miltiorrhiza Bge., is a traditional and folk medicine in Asian countries, especially in China and Japan. In this review, we summarized the recent researches of Danshen in traditional uses and preparations, chemical constituents, pharmacological activities and side effects. A total of 201 compounds from Danshen have been reported, including lipophilic diterpenoids, water-soluble phenolic acids, and other constituents, which have showed various pharmacological activities, such as anti-inflammation, anti-oxidation, anti-tumor, anti-atherogenesis, and anti-diabetes. This article intends to provide novel insight information for further development of Danshen, which could be of great value to its improvement of utilization. | MEI Xiao-Dan CAO Yan-Feng CHE Yan-Yun LI Jing SHANG Zhan-Peng ZHAO Wen-Jing QIAO Yan-Jiang ZHANG Jia-Yu | 2019 | Chinese Journal of Natural Medicines2019，17，1： | 43 |
| 4 | Zircon U-Pb geochronological framework of Qitianling granite batholith, middle part of Nanling Range, South China显示文摘The Qitianling granite batholith (QGB) is located in the southern Hunan Province, middle part of the Nanling Range, South China. Its total exposure area is about 520 km2. Based on our 25 single grain zircon U-Pb age data and 7 published data as well as the geological, petrological, and space distribution characteristics, we conclude that QGB is an Early Yanshanian (Jurassic) multi-staged composite pluton. Its formation process can be subdivided into three major stages. The first stage, emplaced at 163―160 Ma with a peak at about 161 Ma, is mainly composed of hornblende-biotite monzonitic granites and locally biotite granites, and distributed in the eastern, northern, and western peripheral parts of the pluton. The second stage, emplaced at 157―153 Ma with a peak at 157―156 Ma, is mainly composed of biotite granites and locally containing hornblende, and distributed in the middle and southeastern parts of the pluton. The third stage, emplaced at 150―146 Ma with a peak at about 149 Ma, is mainly composed of fine-grained (locally porphyritic) biotite granites, and distributed in the middle-southern part of the pluton. Each stage can be further disintegrated into several granite bodies. The first two intrusive stages comprise the major phase of QGB, and the third intrusive stage comprises the additional phase. Many second stage fine-grained granite bosses and dykes intruded into the first stage host granites with clear chilling margin-baking phenomena at their intrusive contacts. They were emplaced in the open fracture space of the earlier stage consolidated rocks. Their isotopic ages are mostly 2―6 Ma younger than their hosts. Conceivably, the time interval from magma emplacement, through cooling, crystallization, solidification, up to fracturing of the earlier stage granites cannot exceed 2―6 Ma. During the Middle-Late Jurassic in the Qitianling area and neighboring Nanling Range, the coeval granitic and basic-intermediate magmatic activities were widely developed. It indicates that the Early Yanshanian period was the culmination time of magmatic activities in this region. The Nanling Range was under a post-orogenic, intracontinental geotectonic environment with an obvious lithospheric extension and thinning. The crust-mantle interaction played an important role in formation of granitic rocks in this region. | ZHU JinChu WANG RuCheng ZHANG PeiHua XIE CaiFu ZHANG WenLan ZHAO KuiDong XIE Lei YANG Ce CHE XuDong YU APeng WANG LuBin | 2009 | Science China Earth Sciences2009，52，9： | 27 |
| 5 | Relative Contributions of Boundary-Layer Meteorological Factors to the Explosive Growth of PM2.5 during the Red-Alert Heavy Pollution Episodes in Beijing in December 2016显示文摘Based on observations of urban mass concentration of fine particulate matter smaller than 2.5 μm in diameter(PM_(2.5)), ground meteorological data, vertical measurements of winds, temperature, and relative humidity(RH), and ECMWF reanalysis data, the major changes in the vertical structures of meteorological factors in the boundary layer(BL) during the heavy aerosol pollution episodes(HPEs) that occurred in winter 2016 in the urban Beijing area were analyzed. The HPEs are divided into two stages: the transport of pollutants under prevailing southerly winds, known as the transport stage(TS), and the PM_(2.5) explosive growth and pollution accumulation period characterized by a temperature inversion with low winds and high RH in the lower BL, known as the cumulative stage(CS). During the TS, a surface high lies south of Beijing, and pollutants are transported northwards. During the CS, a stable BL forms and is characterized by weak winds, temperature inversion, and moisture accumulation. Stable atmospheric stratification featured with light/calm winds and accumulated moisture(RH > 80%) below 250 m at the beginning of the CS is closely associated with the inversion, which is strengthened by the considerable decrease in near-surface air temperature due to the interaction between aerosols and radiation after the aerosol pollution occurs. A significant increase in the PLAM(Parameter Linking Aerosol Pollution and Meteorological Elements) index is found, which is linearly related to PM mass change. During the first 10 h of the CS, the more stable BL contributes approximately 84% of the explosive growth of PM_(2.5) mass. Additional accumulated near-surface moisture caused by the ground temperature decrease, weak turbulent diffusion, low BL height, and inhibited vertical mixing of water vapor is conducive to the secondary aerosol formation through chemical reactions, including liquid phase and heterogeneous reactions, which further increases the PM_(2.5) concentration levels. The contribution of these reaction mechanisms to the explosive growth of PM_(2.5) mass during the early CS and subsequent pollution accumulation requires further investigation. | junting zhong xiaoye zhang yaqiang wang junying sun yangmei zhang jizhi wang kaiyan tan xiaojing shen haochi che lu zhang zhouxiang zhang xuefei qi huarong zhao sanxue ren yang li | 2017 | Journal of Meteorological Research2017，31，5： | 25 |
| 6 | Effects of dietary Clostridium butyricum supplementation on growth performance,intestinal development,and immune response of weaned piglets challenged with lipopolysaccharide显示文摘Background: Weanling pigs, with immature immune system and physiological function, usually experience postweaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, and immunity of weaned pigs challenged with lipopolysaccharide(LPS).Methods: In Experiment(Exp.) 1,144 weaned piglets were weaned at 21 d and randomly assigned to six groups,with six replicates per group and four pigs per replicate, receiving a control diet(CON) or diet supplemented with antibiotics(AB) or C. butyricum(CB)(0.1%, 0.2%, 0.4%, or 0.8%), respectively. All diets in Exp. 1 were a highly digestible basal diet, with 3,000 mg/kg zinc oxide supplied in the first 2 wk only. In Exp. 2, 180 piglets were weaned at 21 d and randomly assigned to five groups, with six replicates per group and six pigs per replicate, receiving CON, AB, or CB(0.2%, 0.4%, or 0.6%) diets. The digestibility of diets was lower than those in Exp. 1, and did not include zinc oxide. At 36 d of Exp. 2, 12 piglets were selected from each of the CON and 0.4% CB groups, six piglets were intraperitoneally injected with LPS(50 μg/kg body weight) and the other six piglets with normal saline;animals were killed at 4 h after injection to collect blood, intestine, and digesta samples for biochemical analysis.Results: In Exp. 1, CB and AB diets had no effect on growth performance of piglets. In Exp. 2, 0.4% CB decreased feed-gain ratio(P < 0.1), diarrhea score(P < 0.05), and increased duodenal, jejunal, and ileal villus height and jejunal villus height/crypt depth(P < 0.05). The 0.4% CB decreased the plasma tumor necrosis factor(TNF) α(P < 0.05) but increased ileal mucosa IL-10 and TLR2 mRNA expression(P < 0.05). Furthermore, 0.4% CB altered the microbial profile, with Bacillus and Ruminococcaceae UGG-003 at genus level and Lactobacillus casei and Parasutterella secunda at species level were higher than CON in colonic content(P < 0.05).Conclusions: Dietary C. butyricum supplementation had positive effects on growth of weaned piglets with less digestible diets. There was a tendency to reduce the feed-gain ratio, which could reduce feed costs in pig production. Moreover, C. butyricum decreased post-weaning diarrhea by improving the intestinal morphology,intestinal microflora profile, and immune function. | Ling Chen Shuang Li Jie Zheng Wentao Li Xuemei Jiang Xilun Zhao Jian Li Lianqiang Che Yan Lin Shengyu Xu Bin Feng Zhengfeng Fang De Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 26 |
| 7 | Observation and analysis of near-surface atmospheric aerosol optical properties in urban Beijing显示文摘Year-round measurements of the mass concentration and optical properties of fine aerosols(PM_(2.5)) from June 2009 to May 2010 at an urban site in Beijing were analyzed.The annual mean values of the PM_(2.5) mass concentration,absorption coefficient(Ab),scattering coefficient(Sc) and single scattering albedo(SSA)at 525 nm were 67±66μg/m^3,64 ±62 Mm^(-1),360 ±405 Mm^(-1) and 0.82 ±0.09,respectively.The bulk mass absorption efficiency and scattering efficiency of the PM_(2.5) at 525 nm were 0.78 m^2/g and 5.55 m^2/g,respectively.The Ab and Sc showed a similar diurnal variation with a maximum at night and a minimum in the afternoon,whereas SSA displayed an opposite diurnal pattern.Significant increases in the Ab and Sc were observed in pollution episodes caused by the accumulation of pollutants from both local and regional sources under unfavorable weather conditions.Aerosol loadings in dust events increased by several times in the spring,which had limited effects on the Ab and Sc due to the low absorption and scattering efficiency of dust particles.The frequency of haze days was the highest in autumn because of the high aerosol absorption and scattering under unfavorable weather conditions.The daily PM_(2.5)concentration should be controlled to a level lower than 64 μg/m^3 to prevent the occurrence of haze days according to its exponentially decreased relationship with visibility. | Junshan Jing Yunfei Wu Jun Tao Huizheng Che Xiangao Xia Xiaochun Zhang Peng Yan Deming Zhao Leiming Zhang | 2015 | Particuology2015，13，1： | 16 |
| 8 | Clinical application and mechanism of traditional Chinese medicine in treatment of lung cancer显示文摘Lung cancer is a malignant tumor characterized by a rapid proliferation rate,less survivability,high mortality,and metastatic potential.This review focuses on updated research about the clinical application of traditional Chinese medicine(TCM)as an adjuvant therapy to lung cancer treatment and the mechanisms of TCM effect on lung cancer in vitro and in vivo.We summarized the recent 5 years of different research progress on clinical applications and antitumor mechanisms of TCM in the treatment of lung cancer.As a potent adjuvant therapy,TCM could enhance conventional treatments(chemotherapy,radiation therapy,and epidermal growth factor receptors[EGFRs]tyrosine kinase inhibitors[TKIs])effects as well as provide synergistic effects,enhance chemotherapy drugs chemosensitivity,reverse drug resistance,reduce adverse reactions and toxicity,relieve patients’pain and improve quality of life(QOL).After treating with TCM,lung cancer cells will induce apoptosis and/or autophagy,suppress metastasis,impact immune reaction,and therapeutic effect of EGFR-TKIs.Therefore,TCM is a promisingly potent adjuvant therapy in the treatment of lung cancer and its multiple mechanisms are worthy of an in-depth study. | Xiao-Lin Su Jiang-Wei Wang Hui Che Chang-Fu Wang Hai Jiang Xia Lei Wan Zhao Hai-Xue Kuang Qiu-Hong Wang | 2020 | Chinese Medical Journal2020，，24： | 16 |
| 9 | Integral stormwater management master plan and design in an ecological community显示文摘Urban stormwater runoff nearly discharges directly into bodies of water through gray infrastructure in China,such as sewers,impermeable ditches,and pump stations.As urban flooding,water shortage,and other environment problems become serious,integrated water environment management is becoming increasingly complex and challenging.At more than 200 ha,the Oriental Sun City community is a large retirement community located in the eastern side of Beijing.During the beginning of its construction,the project faced a series of serious water environment crises such as eutrophication,flood risk,water shortage,and high maintenance costs.To address these issues,an integral stormwater management master plan was developed based on the concept of low impact development(LID).A large number of LID and green stormwater infrastructure(GSI)approaches were designed and applied in the community to replace traditional stormwater drainage systems completely.These approaches mainly included bioretention(which captured nearly 85th percentile volume of the annual runoff in the site,nearly 5.4×105m3annually),swales(which functioned as a substitute for traditional stormwater pipes),waterscapes,and stormwater wetlands.Finally,a stormwater system plan was proposed by integrating with the gray water system,landscape planning,an architectural master plan,and related consultations that supported the entire construction period.After more than 10 years of planning,designing,construction,and operation,Oriental Sun City has become one of the earliest modern large-scale LID communities in China.Moreover,the project not only addressed the crisis efficiently and effectively,but also yielded economic and ecological benefits. | Wu Che Yang Zhao Zheng Yang Junqi Li Man Shi | 2014 | Journal of Environmental Sciences2014，26，9： | 13 |
| 10 | Review grain yield and nitrogen use efficiency in rice production regions in China显示文摘As one of the staple food crops, rice(Oryza sativa L.) is widely cultivated across China, which plays a critical role in guaranteeing national food security. Most previous studies on grain yield or/and nitrogen use efficiency(NUE) of rice in China often involved site-specific field experiments, or small regions with insufficient data, which limited the representation for the current rice production regions. In this study, a database covering a wide range of climate conditions, soil types and field managements across China, was developed to estimate rice grain yield and NUE in various rice production regions in China and to evaluate the relationships between N rates and grain yield, NUE. According to the database for rice, the values of grain yield, plant N accumulation, N harvest index(HIN), indigenous N supply(INS), internal N efficiency(IE_N), reciprocal internal N efficiency(RIE_N), agronomic N use efficiency(AE_N), partial N factor productivity(PEPN), physiological N efficiency(PE_N), and recover efficiency of applied N(RE_N) averaged 7.69 t ha^(–1), 152 kg ha^(–1), 0.64 kg kg^(–1), 94.1 kg kg^(–1), 53.9 kg kg^(–1), 1.98 kg kg^(–1), 12.6 kg kg^(–1), 48.6 kg kg^(–1), 33.8 kg kg^(–1), and 39.3%, respectively. However, the corresponding values all varied tremendously with large variation. Rice planting regions and N rates had significant influence on grain yield, N uptake and NUE values. Considering all observations, N rates of 200 to 250 kg ha^(–1) commonly achieved higher rice grain yield compared to less than 200 kg N ha^(–1) and more than 250 kg N ha^(–1) at most rice planting regions. At N rates of 200 to 250 kg ha^(–1), significant positive linear relationships were observed between rice grain yield and AE_N, PE_N, RE_N, IE_N, and PFPN, and 46.49, 24.64, 7.94, 17.84, and 88.24% of the variation in AE_N, PE_N, RE_N, IE_N, and PFPN could be explained by grain yield, respectively. In conclusion, in a reasonable range of N application, an increase in grain yield can be achieved accompanying by an acceptable NUE. | CHE Sheng-guo ZHAO Bing-qiang LI Yan-ting YUAN Liang LI Wei LIN Zhi-an HU Shu-wen SHEN Bing | 2015 | Journal of Integrative Agriculture2015，14，12： | 12 |
| 11 | The roles of surfactant protein D during Aspergillus fumigatus infection in human corneal epithelial cells显示文摘AIM: To investigate roles of surfactant protein D (SP-D) and relative cytokines in human corneal epithelial(HCE) cells exposed to aspergillus fumigatus (AF) antigens.METHODS: HCE cells cultured in vitro with AF antigens and sampled at 0, 0.5, 1 hour, 2, 4, 6 and 8 hours. The expression of SP-D mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).The expression of SP-D protein was shown by ELISA and immunocytochemistry SP methods. The expression of NF-κB and relative downstream cytokines such as TNF-α, IL-1β, IL-8 and IL-10 in supernatant fluid were measured by ELISA.RESULTS: SP-D mRNA and protein were detected in untreated HCE cells. The expression of SP-D and the relative downstream cytokines rose after being stimulated with AF antigens. SP-D mRNA began to rise at 0.5 hour and the most significantly peak was in 2 hours. The protein of SP-D in supernatant fluid had the same trend with mRNA. Immunocytochemistry of SP-D showed positive expression and gradually increased to 6 hours, and then the expression began to decline. NF-κB was activated after treated by AF antigens and the changes had correlation with SP-D. TNF-α, IL-1β, IL-8 and IL-10 began to rise after given AF antigens 1 hour and were 1.82, 1.43, 1.12 and 1.28 times higher than the untreated HCE cells separately. The expression of TNF-α and IL-1β reached the peak at 2 hours, separately 2.80 and 2.86 times than the untreated. The expression of IL-8 and IL-10 gradually increased with a time-dependent manner.CONCLUSION: HCE cells exists SP-D and it may play a significant role in pathogenesis of keratomycosis. AF may induce human corneal epithelial cells to express inflammatory cytokines via SP-D and NF-κB pathway. SP-D possibly mediates the recognition to AF mycelium. | Cheng-Ye Che Wen-Yan Jia Qiang Xu Na Li Li-Ting Hu Nan Jiang Jing Lin Qing Wang and Gui-Qiu Zhao | 2012 | International Journal of Ophthalmology(English edition)2012，5，1： | 12 |
| 12 | Boxb mediate BALB/c mice corneal inflammation through a TLR4/MyD88-dependent signaling pathway in Aspergillus fumigatus keratitis显示文摘AIM: To investigate whether high-mobility group box 1(HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4(TLR4)/myeloid differentiation primary response 88(My D88)-dependent signaling pathway in Aspergillus fumigatus(A. fumigatus) keratitis.METHODS: The mice corneas were pretreated with phosphate buffer saline(PBS), Boxb before A. fumigatus infection. The abdominal cavity extracted macrophages were pretreated with PBS, Boxb, TLR4 inhibitor(CLI-095), Dimethyl sulfoxide(DMSO) separately before A. fumigatus hyphae stimulation. HMGB1 was detected in normal and infected mice corneas and macrophages by real-time reverse transcriptase polymerase chain reaction(RT-PCR), the TLR4, My D88, interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) were detected by Western blot and PCR.RESULTS: In BALB/c mice corneas, the expressions of TLR4, HMGB1, IL-1β, TNF-α were increased after A. fumigatus infection. While pretreatment with Boxb significantly increased the expressions of TLR4, HMGB1, My D88, IL-1β, TNF-α compared with PBS control after infection. In BALB/c mice abdominal cavity extracted macrophages, pretreatment with Boxb increased the expressions of TLR4, HMGB1, My D88, IL-1β, TNF-α, while pretreatment with CLI-095 and Boxb significantly decreased the expressions of TLR4, HMGB1, My D88, IL-1β, TNF-α. CONCLUSION: In A. fumigatus keratitis, Boxb play a proinflammatory role in corneal anti-fungi immune response through the HMGB1-TLR4-My D88 signal pathway. | Min Liu Cui Li Gui-Qiu Zhao Jing Lin Cheng-Ye Che Qiang Xu Qian Wang Rui Xu Ya-Wen Niu | 2018 | International Journal of Ophthalmology(English edition)2018，11，4： | 10 |
| 13 | β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation显示文摘AIM:To explore the potential of β-elemene as a radiosensitizer for gastric cancer cells and the underlying mechanisms.METHODS:SGC7901,MKN45,MKN28,N87,and AGS human gastric cancer cell lines were used to screen for radioresistant gastric cancer cell lines. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) assay was used to determine the effects of β-elemene and IPA-3 on cell viability in MKN45 and SGC7901 gastric cancer cell lines. A clonogenic survival assay and annexin V-FITC/PI apoptosis detection assay were used to evaluate cellular radiosensitivity and radiation-induced cell death,respectively. A proteomic method,isobaric tags for relative and absolute quantitation(i TRAQ),was employed to screen the proteins regulated by β-elemene pretreatment prior to ionizing radiation(IR) in SGC7901 gastric cancer cell line. IPA-3 was used as a specific small molecule inhibitor of p21-activated protein kinase 1(Pak1) to target Pak1 signaling. Protein levels of PAK1IP1(p21-activated protein kinase-interacting protein 1),total Pak1(t-Pak1),phospho-Pak1(T423),phospho-ERK1/2( Thr202/Tyr204),and cleaved caspase-3(17 k Da) were assessed by western blotting.RESULTS:MKN45 and SGC7901 gastric cancer cell lines were relatively more resistant to IR. β-elemene pretreatment decreased clonogenic survival following IR in MKN45 and SGC7901 gastric cancer cell lines. Additionally,β-elemene pretreatment prior to IR increased radiation-induced cell death compared with IR alone in MKN45(10.4% ± 0.9% vs 34.8% ± 2.8%,P < 0.05) and SGC7901(11.6% ± 0.9% vs 46.7% ± 5.2%,P < 0.05) human gastric cancer cell lines,respectively,consistent with the level of cleaved caspase-3(17 k Da). Through i TRAQ analysis and western blot validation,we found that β-elemene upregulated PAK1IP1 and downregulated phospho-Pak1(T423) and phosphoERK1/2 in SGC7901 gastric cancer cells. IR increased the level of phospho-Pak1(T423). Pretreatment with β-elemene decreased radiation-induced Pak1 and ERK1/2 phosphorylation. Inhibition of Pak1 using IPA-3 decreased clonogenic survival following IR. In addition,IPA-3 increased radiation-induced cell death in MKN45(13.4% ± 0.3% vs 26.6% ± 1.0%,P < 0.05) and SGC7901(16.0% ± 0.6% vs 37.3% ± 1.7%,P < 0.05) gastric cancer cell lines,respectively,consistent with the level of cleaved caspase-3(17 k Da). Western blotting showed that IPA-3 decreased radiation-induced Pak1 and ERK1/2 phosphorylation.CONCLUSION:This is the first demonstration that β-elemene enhances radiosensitivity of gastric cancer cells,and that the mechanism involves inhibition of Pak1 signaling. | Jun-Song Liu Xiang-Ming Che Shuai Chang Guang-Lin Qiu Shi-Cai He Lin Fan Wei Zhao Zheng-Liang Zhang Shu-Feng Wang | 2015 | World Journal of Gastroenterology2015，21，34： | 9 |
| 14 | Comparative Analysis of Nutrient Composition of Caulerpa lentillifera from Different Regions显示文摘This study investigated the difference of nutrient composition in Caulerpa lentillifera collected from different regions.The nutrient compositions of C.lentillifera from China's Hainan and Shandong provinces were determined and compared with those from Semporna(Malaysia),Sabah(Malaysia),Petchburi(Thailand),and two other species of seaweeds(Malaysia).The results showed that the polysaccharide and protein contents of C.lentillifera from Hainan(44.82%±0.98%and 12.5%±0.70%),Shandong(43.22%±1.42%and 14.7%±0.72%),Petchburi(59.27%±2.31%and 12.49%±0.30%),and Sabah(44.02%±2.01%and 19.38%±1.48%)were higher than those of Eucheuma cottonii(26.49%±3.01%and 9.76%±1.33%)and Sargassum polycystum(33.49%±1.70%and 5.40%±0.07%),respectively.The polyunsaturated fatty acid contents in C.lentillifera from Hainan(29.98%)and Shandong(22.11%)were higher than that in Semporna(16.76%±0.27%),Sabah(9.49%),and S.polycystum(20.34%±0.43%),but lower than that in E.cottonii(51.55%±0.57%).In Caulerpa lentillifera of Hainan and Shandong provinces,the essential amino acid(EAA)/total amino acid(TAA)ratios were 35.22%and 38.78%,respectively;and the EAA/ETAA ratios were 54.36%and 63.35%,respectively.The EAA composition of C.lentillifera was close to the ideal model of Food and Agriculture Organization/World Health Organization.C.lentillifera was rich in mineral elements,including calcium((3315.85±127.55)mg(100 g)^-1 and(3728.35±92.38)mg(100 g)^-1),magnesium((6715.74±82.58)mg(100 g)^-1 and(8128.59±242.72)mg(100 g)^-1),and trace elements,including iron((510.65±5.47)mg(100 g)^-1 and(1972.97±183.35)mg(100 g)^-1),selenium((1.04±0.08)mg(100 g)^-1 and(0.83±0.15)mg(100 g)^-1),and zinc((33.90±0.13)mg(100 g)^-1 and(11.75±2.05)mg(100 g)^-1).These results showed that C.lentillifera was more nutritious than S.polycystum or E.cottonii in terms of polysaccharide,protein,and fatty acid contents.Furthermore,both C.lentillifera species collected from Hainan and Shandong provinces show more basic nutrients.Therefore,C.lentillifera has important development and application prospects. | ZHANG Meijian MA Yurong CHE Xinyi HUANG Zumei CHEN Peng XIA Guanghua ZHAO Meihui | 2020 | Journal of Ocean University of China2020，19，2： | 8 |
| 15 | Effect of sevoflurane on tissue permeability of lung ischemia-reperfusion injury in rats显示文摘Objective:To investigate the effect of sevoflurane on tissue permeability of lung ischemiareperfusion injury(LIRI)in rats.Methods:A total of 45 wistar rats were randomly divided into3 groupsⅠ,Ⅱ,Ⅲ.Modified Eppinger method was adopted to establish the rat lung ischemiareperfusion injury model.GroupⅠserved as the control group,groupⅡas ischemia reperfusion group,groupⅢas sevoflurane ischemia-reperfusion group.Blood gas index,lung permeability index(LPI)change,lung tissue pathology change and lung water content were observed and compared between groups of rats at different time points.Results:During ischemia reperfusion,all rats kept balance of the MAP during different time points,SPO_2 of groupⅡandⅢdecreased significantly thanⅠgroup(P<0.05);after reperfusion lung permeability index in GroupⅡandⅢwas higher than the control group significantly(P<0.05),120 min after reperfusion LPI change and iujury of groupⅢwas significantly lower thanⅡgroup(P<0.05);interstitial and alveolar cavity effusion in of groupⅢwere lower than that of groupⅡ.Conclusions:Sevoflurane pretreatment can reduce the lung tissue permeability,and LIRI plays a protective role in LIRI. | Xiao-Hui Li Zhong-Hua Liu Hong-Bing Ma Yong Li Hui Zhao Jian-Bo Che Wei-Chao Liu Gong-Ning Shi | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，4： | 8 |
| 16 | Effects of dietary supplementation with essential oils and protease on growth performance,antioxidation,inflammation and intestinal function of weaned pigs显示文摘This experiment evaluated the impacts of essential oils(EO)and protease(PRO),independently or in combination,on growth performance,antioxidation,inflammation and intestinal function of weaned pigs.One hundred and sixty weaned pigs(21 d of age,BW of 6.74±0.20 kg)were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen.Dietary treatments included the following:1)control diet(CON),2)CON with 300 mg/kg essential oils(EO),3)CON with 500 mg/kg protease(PRO),4)CON with 300 mg/kg essential oil and 500 mg/kg protease(EO + PRO).On d 8,one pig from each penwas selected for sampling.The remaining pigs were fed for an additional week and growth performance was monitored during this period.Dietary treatments had no marked effects(P>0.05)on the growth performance of pigs.However,pigs receiving EO diet had higher(P<0.05)serumglutathione peroxidase(GSH-Px)activity,and tended to decrease(P=0.063)serum concentration of tumor necrosis factor-a(TNF-a).In addition,pigs receiving EO diet had higher(P<0.05)abundances of phylum Actinobacteria,and genera Bifidobacterium,and lower(P<0.05)phylum Bacteroidetes and genera Alloprevotella in colonic digesta.Pigs receiving PRO diet decreased(P<0.05)the serum concentration of malondialdehyde(MDA)and diamine oxidase activity,increased(P<0.05)the villus height and the ratio of villus height to crypt depth in duodenum,increased sucrase activity in jejunal mucosa,and also increased the abundance of phylum Actinobacteria in colonic digesta.Furthermore,the synergistic effects of EO and PROwas observed(P<0.05)for pigs with decreasing serum TNF-a concentration and increasing serum GSH-Px activity.Collectively,the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs.EO diet appeared to improve antioxidant activity and intestinal microbiota,while PRO diet improved intestinal morphology and digestive enzyme activity,and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs. | Xie Peng Qiang Zhou Cheng Wu Jian Zhao Quan Tan Ying He Liang Hu Zhengfeng Fang Yan Lin Shengyu Xu Bin Feng Jian Li Yong Zhuo Chris Van Ginneken Xuemei Jiang De Wu Lianqiang Che | 2022 | Animal Nutrition2022，，2： | 8 |
| 17 | Anorectal malignant melanomas: Retrospective experience with surgical management显示文摘AIM: To present the experience and outcomes of the surgical treatment for the patients with anorectal melanoma from the Cancer Hospital, Chinese Academy of Medical Sciences. METHODS: Medical records of the diagnosis, surgery, and follow-up of 56 patients with anorectal melanoma who underwent surgery between 1975 and 2008 were retrospectively reviewed. The factors predictive for the survival rate of these patients were identified using multivariate analysis. RESULTS: The 5-year survival rate of the 56 patients with anorectal melanoma was 20%, 36 patients underwent abdominoperineal resection (APR) and 20 patients underwent wide local excision (WLE). The rates of local recurrence of the APR and WLE groups were 16.13% (5/36) and 68.75% (13/20), (P = 0.001), and the median survival time was 22 mo and 21 mo, respectively (P = 0.481). Univariate survival analysis demonstrated that the number of tumor and the depth of invasion had significant effects on the survival (P < 0.05). Multivariate analysis showed that the number of tumor [P = 0.017, 95% confidence interval (CI) = 1.273-11.075] and the depth of invasion (P = 0.015, 95% CI = 1.249-7.591) were independent prognostic factors influencing the survival rate. CONCLUSION: Complete or R0 resection is the first choice of treatment for anorectal melanoma, prognosis is poor regardless of surgical approach, and early diagnosis is the key to improved survival rate for patients with anorectal melanoma. | Xu Che Dong-Bing Zhao Yong-Kai Wu Cheng-Feng Wang Jian-Qiang Cai Yong-Fu Shao Ping Zhao | 2011 | World Journal of Gastroenterology2011，17，4： | 7 |
| 18 | Numerical simulation of an acoustic field generated by a phased arc array in a fluid-filled cased borehole显示文摘Transducers that are widely applied in cement bond evaluation tools, such as cement bond logs and variable density logs, cannot radiate acoustic energy directionally because of the characteristics of monopole sources. A phased arc array transmitter, which is a novel transducer that differs from monopole and dipole transducers, is presented in this study. To simulate the acoustic field generated by a phased arc array in a fluid-filled cased borehole with different channelings, a 3D finite-difference time-domain method is adopted. The acoustic field generated by a traditional monopole source is also simulated and compared with the field generated by the phased arc array transmitter. Numerical simulation results show that the phased arc array radiates energy directionally in a narrow angular range in the borehole, thereby compressing the acoustic energy into a narrow range in the casing pipe, the cement, and the formation. We present the analyses of first-arrival waveforms and the amplitudes of casing waves at different azimuthal angles for the two different sources. The results indicate that employing a directional source facilitates azimuthal identification and analysis of possible channeling behind the casing pipe. | Che Xiaohua Qiao Wenxiao Wang Ruijia Zhao Yuhong | 2014 | Petroleum Science2014，11，3： | 7 |
| 19 | Body mass index does not affect the survival of pancreatic cancer patients显示文摘AIM to evaluate the association of body mass index(b MI) with the overall survival of pancreatic ductal adenocarcinoma(PDAC) patients.METHODS A retrospective analysis of PDAC patients diagnosed in the National Cancer Center of China between January 1999 and December 2014 was performed. these patients were categorized into four b MI groups(< 18.5, 18.5-22.9, 23-27.4 and ≥ 27.5 kg/m2). χ2 tests for comparison of the proportions of categorical variables, and Student's t-test or Mann-Whitney test for continuous variables were employed. Survival analysis was performed with the Kaplan-Meyer method. their HRs of mortality and 95%CIs were estimated using the Cox proportional hazards model.RESULTS With a median age of 59.6 years(range: 22.5-84.6 years), in total 1783 PDAC patients were enrolled in this study. their mean usual b MI was 24.19 ± 3.53 for the whole cohort. More than half of the patients(59.3%) experienced weight loss during the disease onset and progression. Compared with healthy-weight individuals, newly diagnosed patients who were overweight or obese had more severe weight loss during their disease onset and progression(P < 0.001). Individuals who were overweight or obese were associated with positive smoking history(P < 0.001). A significant difference in comorbidity of diabetes(P = 0.044) and coronary artery disease(P < 0.001) was identified between high b MI and normal-weight patients. After a median follow-up of 8 mo, the survival analysis showed no association between b MI and the overall survival(P = 0.90, n = 1783). When we stratified the whole cohort by pancreatic cancer stage, no statistically significant association between b MI and overall survival was found for resectable(P = 0.99, n = 217), unresectable locally advanced(P = 0.90, n = 316) and metastatic patients(P = 0.88, n = 1250), respectively. the results did not change when we used the b MI at diagnosis.CONCLUSION Our results showed no significance of b MI for the overall survival of PDAC patients. | Qing-long Jiang Cheng-feng Wang Yan-tao tian Huang Huang Shui-Sheng Zhang Dong-bing Zhao Jie Ma Wei Yuan Yue-Min Sun Xu Che Jian-Wei Zhang Yun-Mian Chu Ya-Wei Zhang Ying-tai Chen | 2017 | World Journal of Gastroenterology2017，23，34： | 7 |
| 20 | Endothelial cell-specific anticoagulation reduces inflammation in a mouse model of acute lung injury显示文摘Tissue factor (TF)-dependent coagulation contributes to lung inflammation and the pathogenesis of acute lung injury (ALI). In this study, we explored the roles of targeted endothelial anticoagulation in ALI using two strains of transgenic mice expressing either a membrane-tethered human tissue factor pathway inhibitor (hTFPI) or hirudin fusion protein on CD31^+ cells, including vascular endothelial cells (ECs). ALI was induced by intratracheal injection of LPS, and after 24 h the expression of TF and protease-activated receptors (PARs) on EC in lungs were assessed, alongside the extent of inflammation and injury. The expression of TF and PARs on the EC in lungs was upregulated after ALI. In the two strains of transgenic mice, expression of either of hTFPI or hirudin by EC was associated with significant reduction of inflammation, as assessed by the extent of leukocyte infiltration or the levels of proinflammatory cytokines, and promoted survival after LPS-induced ALI. The beneficial outcomes were associated with inhibition of the expression of chemokine CCL2 in lung tissues. The protection observed in the CD31-TFPI-transgenic strain was abolished by injection of an anti-hTFPI antibody, but not by prior engraftment of the transgenic strains with WT bone marrow, confirming that the changes observed were a specific transgenic expression of anticoagulants by EC. These results demonstrate that the inflammation in ALI is TF and thrombin dependent, and that expression of anticoagulants by EC significantly inhibits the development of ALI via repression of leukocyte infiltration, most likely via inhibition of chemokine gradients. These data enhance our understanding of the pathology of ALI and suggest a novel therapeutic strategy for treatment. | Jian Lou Yue Hu Min-dan Wu Luan-qing Che Yin-fang Wu Yun Zhao Bao-ping Tian Zheng-qiang Bao Chen Zhu Yan-ping Wu Lu-lu He Chun-xue Bai Jian Zhou Song-min Ying Wen Li Zhi-hua Chen Da-xin Chen Anthony Dorling Hua-hao Shen | 2019 | Acta Pharmacologica Sinica2019，40，6： | 7 |
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