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| 1 | XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice显示文摘 | Chunlian Wang Xiaoping Zhang Yinglun Fan Ying Gao Qinlong Zhu Chongke Zheng Tengfei Qin Yanqiang Li Jinying Che Mingwei Zhang Bing Yang Yaoguang Liu Kaijun Zhao | 2015 | Molecular Plant2015，8，2： | 55 |
| 2 | Cyclometalated platinum(Ⅱ) complexes with sterically bulky camphor-derived groups as β-diketonate ancillary ligand:a new route to efficiently reducing π-π interactions and Pt-Pt interactions显示文摘A new series of mono-cyclometalated square planar platinum(Ⅱ) complexes have been synthesized and the single-crystal X-ray structures of complex 1 and 2 have been determined.The complexes have the general formula ppyPt(O^O),where ppy is 2-phenylpyridyl,and O^O is β-diketonate ancillary ligands with the acyl substituent group in position 3 of(D)-(+)-camphor.Although,like the many Pt(Ⅱ) complexes with square-planar geometry,these complexes have plane stacking modes in crystal structure,the sterically bulky camphor-derived groups compel extensive slipping of the molecular stacking planes,resulting in the negligible overlapping of the aromatic ring fragments between molecules and the considerable Pt-Pt distance.The resolved spectra and a little shifted emission in solid state of complexes show that there is significant reduction of π-π interactions and Pt-Pt interactions,and suggest these complexes may be good candidates for doped phosphorescent organic light emitting diodes(PhOLEDs) and even for nondoped PhOLEDs. | LUO KaiJun,XIE Yu,XU LingLing,WANG JiangWei,ZHANG LiFang & ZHAO KeQing College of Chemistry and Materials Science,Sichuan Normal University,Chengdu 610068,China | 2010 | Science China Chemistry2010，53，1： | 5 |
| 3 | HrcQ is necessary for Xanthomonas oryzae pv. oryzae HR-induction in non-host tobacco and pathogenicity in host rice显示文摘Bacterial blight, caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice(Oryza sativa L.) worldwide. The type III secretion system(T3SS) of Xoo, encoded by the hrp(hypersensitive response and pathogenicity) genes, plays critical roles in conferring pathogenicity in host rice and triggering a hypersensitive response(HR) in non-host plants. To investigate the major genes conferring the pathogenicity and avirulence of Xoo, we previously constructed a random Tn5-insertion mutant library of Xoo strain PXO99A. We report here the isolation and characterization of a Tn5-insertion mutant PXM69. Tn5-insertion mutants were screened on indica rice JG30, which is highly susceptible to PXO99A, by leaf-cutting inoculation.Four mutants with reduced virulence were obtained after two rounds of screening. Among them, the mutant PXM69 had completely lost virulence to the rice host and ability to elicit HR in non-host tobacco. Southern blotting analysis showed a single copy of a Tn5-insertion in the genome of PXM69. PCR walking and sequencing analysis revealed that the Tn5 transposon was inserted at nucleotide position 70,192–70,201 in the genome of PXO99A, disrupting the type III hrc(hrp-conserved) gene hrcQ, the first gene in the D operon of the hrp cluster in Xoo. To confirm the relationship between the Tn5-insertion and the avirulence phenotype of PXM69, we used the marker exchange mutagenesis to create a PXO99Amutant, ΔhrcQ::KAN, in which the hrcQ was disrupted by a kanamycin-encoding gene cassette at the same site as that of the Tn5-insertion. ΔhrcQ::KAN showed the same phenotype as mutant PXM69. Reintroduction of the wild-type hrcQ gene partially complemented the pathogenic function of PXM69. RT-PCR and cellulase secretion assays showed that the Tn5-disruption of hrcQ did not affect transcription of downstream genes in the D operon and function of the type II secretion system. Our results provide new insights into the pathogenic functions of clustered hrp genes in Xoo. | Xiaoping Zhang Chunlian Wang Chongke Zheng Jinying Che Yanqiang Li Kaijun Zhao | 2013 | The Crop Journal2013，1，2： | 4 |
| 4 | Engineering Broad-Spectrum Resistance to Bacterial Blight by CRISPR/Cas9-Mediated Precise Homology Directed Repair in Rice显示文摘Dear Editor,Rice(Oryza sativa)is one of the most important food crops as well as a model plant for basic research.Rice bacterial blight(BB)caused by Xanthomonas oryzae pv.oryzae(Xoo)is a devastating bacterial disease worldwide(Mew et al.,1993),affecting millions of hectares of rice annually,with an estimated crop loss of as high as 75%.Upon invasion of rice cells,Xoo secretes transcription activator-like effectors(TALEs)to activate the host Sugars Will Eventually be Exported Transporters(SWEET)genes to obtain nutrition for bacterial growth,because the induced SWEET transports more sugars to where the bacteria reside(Boch and Bonas,2010). | Zheng Wei Mohamed Abdelrahman Ying Gao Zhiyuan Ji Rukmini Mishra Hongda Sun Yi Sui Chuanyin Wu Chunlian Wang Kaijun Zhao | 2021 | Molecular Plant2021，14，8： | 3 |
| 5 | High-resolution genetic mapping of rice bacterial blight resistance gene Xa23显示文摘 | Chunlian Wang Yinglun Fan Chongke Zheng Tengfei Qin Xiaoping Zhang Kaijun Zhao | 2014 | Molecular Genetics and Genomics2014，，5： | 3 |
| 6 | Studies of the Geodesic Acoustic Mode Zonal Flow in HL-2A Tokamak Plasmas显示文摘 | ZHAO Kaijun DONG Jiaqi YAN Longwen HONG Wenyu QIAN Jun CHENG Jun YU Deliang YANG Qingwei DING Xuantong LIU Yong PAN Chuanhong | 2006 | Southwestern Institute of Physics Annual Report2006，，1： | 2 |
| 7 | Searchable Encryption System for Big Data Storage显示文摘Big data cloud platforms provide users with on-demand configurable computing,storage resources to users,thus involving a large amount of user data.However,most of the data is processed and stored in plaintext,resulting in data leakage.At the same time,simple encrypted storage ensures the confidentiality of the cloud data,but has the following problems:if the encrypted data is downloaded to the client and then decrypted,the search efficiency will be low.If the encrypted data is decrypted and searched on the server side,the security will be reduced.Data availability is finally reduced,and indiscriminate protection measures make the risk of data leakage uncontrollable.To solve the problems,based on searchable encryption and key derivation,a cipher search system is designed in this paper considering both data security and availability,and the use of a search encryption algorithm that supports dynamic update is listed.Moreover,the system structure has the advantage of adapting different searchable encryption algorithm.In particular,a user-centered key derivation mechanism is designed to realize file-level fine-grained encryption.Finally,extensive experiment and analysis show that the scheme greatly improves the data security of big data platform. | Yuxiang Chen Yao Hao Zhongqiang Yi Kaijun Wu Qi Zhao Xue Wang | 2021 | 国际计算机前沿大会会议论文集2021，，2： | 0 |
| 8 | Mutations of Desmoglein‑2 in Sudden Unexplained Death in the Chinese Han Population显示文摘Sudden unexplained death(SUD)remains a puzzle in forensic medicine.Desmoglein‑2(DSG2)has been linked to arrhythmogenic right ventricular cardiomyopathy which may cause life‑threatening ventricular arrhythmias and sudden death.Fatal arrhythmias resulting in sudden death also occur in the absence of morphologic cardiac abnormalities at autopsy.We hypothesized that DSG2 mutations may be responsible for certain Chinese SUD cases.We sequenced all 15 exons of DSG2 in DNA extracted from postmortem heart tissues of 25 Chinese patients dying from SUD.The primers were designed using the Primer Express 3.0 software.Direct sequencing for both sense and antisense strands was performed with a BigDye Terminator DNA sequencing kit on a 3130 Xl Genetic Analyzer.Mutation damage prediction was made using Mutation Taster,PolyPhen,and SIFT software.In 2 of 25 cases of Chinese SUD samples,two DSG2 heterozygous mutations(p.P927 L and p.T1070M)were identified,and one is probably damaging.We concluded that DSG2 mutations may be related to the occurrence of part of SUD cases in the Chinese Han population. | Junyi Lin Yulei Yang Ziqin Zhao Yiwen Shen Kaijun Ma Mingchang Zhang | 2019 | Journal of Forensic Science and Medicine2019，5，2： | 0 |
| 9 | A self-powered piezoelectret sensor based on foamed plastic garbage for monitoring human motions显示文摘Constructing piezoelectret based on foamed plastic garbage is an advisable strategy for obtaining self-powered flexible electromechanical sensors with good performances.Herein,a self-powered piezoelectret sensor with basic material of low density polyethylene(LDPE)foamed plastic garbage is proposed,with characteristics of easy fabrication,excellent flexibility,and high equivalent piezoelectric coefficient d33 value up to~1,100 pC/N.The output stability is verified by continuously stimulating a sensor for~180,000 cycles under low and high applied pressure,and the variations of peak outputs are less than 5.5%.Applications for measuring low-and high-pressure signals from human body are achieved.Assembled with a wristband,a sensor is demonstrated for detecting the human pulse waves.Moreover,real time human sitting information is wirelessly monitored with a smart chair based on 4 pixels sensors array. | Yujun Shi Kaijun Zhang Sen Ding Zhaoyang Li Yuhao Huang Yucong Pi Dazhe Zhao Yaowen Zhang Renkun Wang Binpu Zhou Zhi-Xin Yang Junwen Zhong | 2023 | Nano Research2023，16，1： | 0 |
| 10 | Assessment of genetic diversity of Xanthomonas oryzae pv.oryzae显示文摘Bacterialblightofrice,causedby Xanthomonasoryzaepv.Oryzae(Xoo.),isoneofthemajorricediseasesinChina.Makingcleartheshiftofgeneticdiversityofthepathogenwillprovideimportantinformationforricebreed ing.Strainscollectedfrom11provinceslocatedinSouth ernregionoftheChangjiangRiverinChinawereassessedby | WANG Chunlian ZHANG Qi ZHOU Yongli ZHAO Bingyu ZHAO Kaijun,Inst of Crop Breeding and Cultivation,Key Lab of Crop Genetic & Breeding of Ministry of Agriculture,CAAS,Beijing 100081,China | 2000 | Chinese Rice Research Newsletter2000，8，4： | 0 |
| 11 | Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal显示文摘Intestinal organoids,derived from intestinal stem cell self-organization,recapitulate the tissue structures and behav-iors of the intestinal epithelium,which hold great potential for the study of developmental biology,disease modeling,and regenerative medicine.The intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut development.However,the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological factors.Here,we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem cells.Furthermore,mechanical stretching stimulates the expansion of SOX9+progenitors by activating the Wnt/β-Catenin signaling.These data demonstrate that the incorporation of mechanical stretch boosts the stemness of intestinal stem cells,thus benefiting organoid growth.Our findings have provided a way to optimize an organoid generation system through understanding cross-talk between biological and mechanical factors,paving the way for the application of mechanical forces in organoid-based models. | Fanlu Meng Congcong Shen Li Yang Chao Ni Jianyong Huang Kaijun Lin Zanxia Cao Shicai Xu Wanling Cui Xiaoxin Wang Bailing Zhou Chunyang Xiong Jihua Wang Bing Zhao | 2022 | Cell Regeneration2022，11，1： | 0 |
| 12 | Atomic-resolution investigation of structural transformation caused by oxygen vacancy in La_(0.9)Sr_(0.1)TiO_(3+δ)titanate layer perovskite ceramics显示文摘Perovskite functional ceramics have been widely applied for thermal protection owing to their unique physical properties.However,formation of oxygen vacancies under external stimuli usually limits their performance in practical applications.Therefore,the mechanism of the effect of oxygen vacancy on the layer structure of perovskite La_(0.9)Sr_(0.1)TiO_(3+δ)was investigated by experiments and first-principles simulations.The experimental results showed that the lattice distortion occurred in oxygen-deficient environment to give a longer c-axis,along with a significant adjustment in the modes of A/B–O bond vibration,resulting in lower reflectivity.Advanced transmission electron microscopy studies revealed that oxygen vacancies induced localized atomic rearrangements via[TiO_(6)]layer movements to adapt to the lattice distortion.This eventually restructured a part of the layer interfaces by expanding the overlapping projection of atoms in the c-axial direction.The specific transformation process was described as a compendious process,while geometric phase analysis effectively clarified how oxygen vacancies can inhibit reflectivity on the layer structure.Thus,this study provides effective approaches for researching the effects of oxygen vacancy on the physical properties of orthorhombic layer perovskite structures,which may facilitate the development of perovskite-based functional devices. | Yang Zhao Yameng Zhu Jinpeng Zhu Hailong Wang Zhuang Ma Lihong Gao Yanbo Liu Kaijun Yang Yongchun Shu Jilin He | 2022 | Journal of Materials Science & Technology2022，，9： | 0 |
| 13 | Three Dimensional Identification of Zonal Flows in the HL-2A Tokamak显示文摘 | YAN Longwen HONG Wenyu LAN Tao ZHAO Kaijun DONG Jiaqi LIU Adi YU Changxuan QIAN Jun CHENG Jun YU Deliang YANG Qingwei DING Xuantong LIU Yong PAN Chuanhong | 2006 | Southwestern Institute of Physics Annual Report2006，，1： | 0 |
| 14 | RAISE:A Resilient Anonymous Information Sharing Environment显示文摘With the widespread application of cloud computing and network virtualization technologies,more and more enterprise applications are directly deployed in the cloud.However,the traditional TCP/IP network transmission model does not fully consider the information security issues caused by the uncontrollable internet environment.Network security communication solutions represented by encrypted virtual private networks(VPN)are facing multiple security threats.In fact,during the communication process,the user application needs to protect not only the content of the communication but also the behavior of the communication,such as the communication relationship,the communication protocol,and so on.Inspired by blockchain and software-defined networking technology,this paper proposes a resilient anonymous information sharing environment,RAISE.The RAISE system consists of user agents,a core switching network and a control cluster based on a consortium blockchain.User agents are responsible for segmenting,encrypting,and encapsulating user traffic.The core switching network forwards user traffic according to the rules issued by the controller,and the controller dynamically calculates the forwarding rules according to the security policy.Different from onion routing technology,RAISE adopts the controller to replace the onion routing model,which effectively overcomes the uncontrollability of nodes.The dispersed computing model is introduced to replace the TCP/IP pipeline transmission models,which overcomes the problems of anti-tracking and traffic hijacking that cannot be solved by VPNs.We propose a blockchain control plane framework,design the desired consensus algorithmand deploy a RAISE systemconsisting of 150 nodes in an internet environment.The experimental results show that the use of blockchain technology can effectively improve the reliability and security of the control plane.While maintaining high-performance network transmission,it further provides network communication security. | Ning Hu Ling Liu Xin Liu Kaijun Wu Yue Zhao | 2023 | Computer Modeling in Engineering & Sciences2023，137，12： | 0 |
| 15 | A Policy Integration Method Based on Multilevel Security for Data Integration显示文摘Integrating and sharing data from different data sources is one of the trends to make better use of data. However,data integration hampers data confidentiality where each data source has its own access control policy. This paper includes a discussion on the issue about access control across multiple data sources when they are combined together in the scenario of searching over these data. A method based on multilevel security for data integration is proposed. The proposed method allows the merging of policies and also tackles the issue of policy conflicts between different data sources. | WANG Xinming TAN Haoxiang CHEN Kaijun TANG Hua ZHAO Gansen TANG Yong NIE Ruihua | 2015 | Wuhan University Journal of Natural Sciences2015，20，6： | 0 |
| 16 | Genome Editing Strategies Towards Enhancement of Rice Disease Resistance显示文摘The emerging pests and phytopathogens have reduced the crop yield and quality, which hasthreatened the global food security. Traditional breeding methods, molecular marker-based breedingapproaches and use of genetically modified crops have played a crucial role in strengthening the foodsecurity worldwide. However, their usages in crop improvement have been highly limited due to multiplecaveats. Genome editing tools like transcriptional activator-like effector nucleases and clustered regularlyinterspaced short palindromic repeats (CRISPR)-associated endonuclease Cas9 (CRISPR/Cas9) haveeffectively overcome limitations of the conventional breeding methods and are being widely accepted forimprovement of crops. Among the genome editing tools, the CRISPR/Cas9 system has emerged as themost powerful tool of genome editing because of its efficiency, amicability, flexibility, low cost andadaptability. Accumulated evidences indicate that genome editing has great potential in improving thedisease resistance in crop plants. In this review, we offered a brief introduction to the mechanisms of differentgenome editing systems and then discussed recent developments in CRISPR/Cas9 system-based genomeediting towards enhancement of rice disease resistance by different strategies. This review also discussed thepossible applications of recently developed genome editing approaches like CRISPR/Cas12a (formerlyknown as Cpf1) and base editors for enhancement of rice disease resistance. | Rukmini MISHRA WEI Zheng Raj Kumar JOSHI ZHAO Kaijun | 2021 | Rice science2021，28，2： | 0 |
| 17 | Effects of snow absence on available N pools and enzyme activities within soil aggregates in a spruce forest on the eastern Tibetan Plateau显示文摘Winter climate change has great potential to affect the functioning of terrestrial ecosystems.In particular,increased soil frost associated with reduced insulating snow cover may impact the soil nitrogen(N)dynamics in cold ecosystems,but little is known about the variability of these effects among the soil aggregates.A snow manipulation experiment was conducted to investigate the effects of snow absence on N cycling within soil aggregates in a spruce forest on the eastern Tibetan Plateau of China.The extractable soil available N(ammonium and nitrate),net N mineralization rate,and N cycling-related enzyme activities(urease,nitrate reductase,and nitrite reductase)were measured in large macroaggregate(>2 mm),small macroaggregate(0.25–2 mm),and microaggregate(<0.25 mm)during the early thawing period in the years of 2016 and 2017.Snow absence increased soil N availabilities and nitrite reductase activity in microaggregate,but did not affect net N mineralization rate,urease or nitrate reductase activities in any of the aggregate fractions.Regardless of snow manipulations,both soil inorganic N and nitrate reductase were higher in small macroaggregate than in the other two fractions.The effect of aggregate size and sampling year was significant on soil mineral N,net N mineralization rate,and nitrite reductase activity.Our results indicated that snow cover change exerts the largest impact on soil N cycling within microaggregate,and its effect is dependent on winter conditions(e.g.,snow cover and temperature).Such findings have important implications for soil N cycling in snow-covered subalpine forests experiencing pronounced winter climate change. | ZhijieLi Rudiger Reichel Zimin Li Kaijun Yang Li Zhang Bo Tan Rui Yin Kerui Zhao Zhenfeng Xu | 2022 | Soil Ecology Letters2022，4，4： | 0 |
| 18 | A climate-resilient R gene in rice traps two pathogen effectors for broad and durable resistance to bacterial blight显示文摘The fast-and ever-growing human population and rapid climate change are threatening the global food security.As a staple food crop,rice(Oryza sativa)feeds nearly half of the world's population.However,bacterial blight(BB),the most devastating bacterial disease of rice(Mew et al.,1993),caused by Xanthomonas oryzae pv.oryzae(Xoo),affects millions of hectares of cultivated rice plants annually,with an estimated crop loss of as high as 75%. | Kaijun Zhao Qi Zhang | 2021 | Molecular Plant2021，14，3： | 0 |
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