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| 1 | Angiogenesis inhibition and cell cycle arrest induced by treatment with Pseudolarix acid B alone or combined with 5-fluorouracil显示文摘与化学疗法的药相结合的 Angiogenesis 禁止者在许多癌症的治疗有重要功效。金钱松属酸 B (PAB ) 是一个传统的终止怀孕的代理人,它以前被显示了减少肿瘤生长和 angiogenesis。在这研究,我们使用了高满意的屏蔽试金在人的脐的静脉 endothelial 房间(HUVEC ) 上检验 PAB 的效果。二个 hepatocarcinoma 22-transplanted 老鼠模型被用来与 5 氟尿嘧啶(5-Fu ) 在联合决定 PAB 功效。我们的结果建议没有明显的 cytotoxicity, PAB (0.1561.250 M ) 在 vitro 以一种集中依赖者方式禁止了 HUVEC 活动性。在 vivo, PAB (25 mg/kg/day ) 在联合治疗支持了 5-Fu (5 mg/kg/2 天) 的反肿瘤功效,导致显著地更高的肿瘤抑制率,更低的 microvessel 密度价值,和延长幸存时间。PAB 由在 G1/S 边界和 M 阶段堵住细胞周期行动了,这也被表明,脉管的 endothelial 生长因素的下面规定,组织缺氧可诱导的因素 1 并且 cdc2 的 cyclin E 表示,和起来规定表示。这些观察提供在有 5-Fu 的联合的 PAB 可能在癌症治疗是有用的第一条证据。 | Jingtao Liu Wei Guo Bo Xu Fuxiang Ran Mingming Chu HongzhengFu Jingrong Cui | 2012 | Acta Biochimica et Biophysica Sinica2012，44，6： | 6 |
| 2 | Impacts of Land Cover Changes on Ecosystem Carbon Stocks Over the Transboundary Tumen River Basin in Northeast Asia显示文摘Understanding the effects of land cover changes on ecosystem carbon stocks is essential for ecosystem management and environmental protection, particularly in the transboundary region that has undergone marked changes. This study aimed to examine the impacts of land cover changes on ecosystem carbon stocks in the transboundary Tumen River Basin(TTRB). We extracted the spatial information from Landsat Thematic Imager(TM) and Operational Land Imager(OLI) images for the years 1990 and 2015 and obtained convincing estimates of terrestrial biomass and soil carbon stocks with the InVEST model. The results showed that forestland, cropland and built-up land increased by 57.5, 429.7 and 128.9 km2, respectively, while grassland, wetland and barren land declined by 24.9, 548.0 and 43.0 km2, respectively in the TTRB from 1990 to 2015. The total carbon stocks encompassing aboveground, belowground, soil and litter layer carbon storage pools have declined from 831.48 Tg C in 1990 to 831.42 Tg C in 2015 due to land cover changes. In detail, the carbon stocks decreased by 3.13 Tg C and 0.44 Tg C in Democratic People's Republic of Korea(North Korea) and Russia, respectively, while increased by 3.51 Tg C in China. Furthermore, economic development, and national policy accounted for most land cover changes in the TTRB. Our results imply that effective wetland and forestland protection policies among China, North Korea, and Russia are much needed for protecting the natural resources, promoting local ecosystem services and regional sustainable development in the transnational area. | XIANG Hengxing JIA Mingming WANG Zongming LI Lin MAO Dehua ZHANG Da CUI Guishan ZHU Weihong | 2018 | Chinese Geographical Science2018，28，6： | 6 |
| 3 | Recombinant rabies virus expressing IL-15 enhances immunogenicity through promoting the activation of dendritic cells in mice显示文摘Rabies remains a public health threat that kills approximately 59,000 people worldwide each year,most of which are from the developing countries of Africa and Asia where dog rabies are endemic.Therefore, developing an affordable and efficacious vaccine is crucial for rabies control in these countries. Interleukin(IL)-15, an immunoregulatory cytokine, is a pluripotent molecule with therapeutic potential, which targets many cell types and links the innate and adaptive immune system. In this study, IL-15 gene was cloned and inserted into the genome of a recombinant rabies virus(RABV) strain LBNSE(designated as LBNSE-IL15), and the effect of over-expression of IL-15 on the immunogenicity of RABV was investigated. It was found that mice vaccinated with LBNSEIL15 could induce significantly higher level of virus-neutralizing antibody(VNA) than those immunized with LBNSE, resulting in the higher protection after challenge. Further investigation was performed to find out the possible role of IL-15 plays in the process of antibody induction, and it was found that LBNSE-IL15 could enhance the maturation of dendritic cells(DCs) in immunized mice. Furthermore, the mice immunized with LBNSE-IL15 could promote the T_(FH) cells differentiation and the generation of germinal center B cells and plasma cells. Together, these data indicated that IL-15 could be a potential adjuvant in enhancing the immunogenicity of RABV, contributing to the development of more-efficacious rabies vaccines. | Tiange Chen Yajing Zhang Zhao Wang Jie Yang Mingming Li Kunlun Wang Min Cui Zhen F.Fu Ling Zhao Ming Zhou | 2017 | Virologica Sinica2017，32，4： | 5 |
| 4 | Hybrid multiple attribute decision making model based on entropy显示文摘From the viewpoint of entropy, this paper investigates a hybrid multiple attribute decision making problem with precision number, interval number and fuzzy number. It defines a new concept: project entropy and the decision is taken according to the values. The validity and scientific nature of the given is proven. | Wang Wei Cui Mingming | 2007 | Journal of Systems Engineering and Electronics2007，18，1： | 4 |
| 5 | A Volumetric Model for Evaluating Tight Sandstone Gas Reserves in the Permian Sulige Gas Field,Ordos Basin,Central China显示文摘To accurately measure and evaluate reserves is critical for ensuring successful production of unconventional oil and gas. This work proposes a volumetric model to evaluate the tight sandstone gas reserves of the Permian Sulige gas field in the Ordos Basin. The reserves can be determined by four major parameters of reservoir cutoffs, net pay, gas-bearing area and compression factor Z, which are controlled by reservoir characteristics and sedimentation. Well logging, seismic analysis, core analysis and gas testing, as well as thin section identification and SEM analysis were used to analyze the pore evolution and pore-throat structure. The porosity and permeability cutoffs are determined by distribution function curve,empirical statistics and intersection plot. Net pay and gas-bearing area are determined based on the cutoffs, gas testing and sand body distribution, and the compression factor Z is obtained by gas component. The results demonstrate that the reservoir in the Sulige gas field is characterized by ultralow porosity and permeability, and the cutoffs of porosity and permeability are 5% and 0.15×10^(–3) μm^2, respectively. The net pay and gas-bearing area are mainly affected by the sedimentary facies, sand body types and distribution. The gas component is dominated by methane which accounts for more than 90%, and the compression factor Z of H_8(P_2h_8) and S_1(P_1s_1) are 0.98 and 0.985, respectively. The distributary channels stacked and overlapped, forming a wide and thick sand body with good developed intergranular pores and intercrystalline pores. The upper part of channel sand with good porosity and permeability can be sweet spot for gas exploration. The complete set of calculation systems proposed for tight gas reserve calculation has proved to be effective based on application and feedback. This model provides a new concept and consideration for reserve prediction and calculation in other areas. | CUI Mingming FAN Aiping WANG Zongxiu GAO Wanli LI Jinbu LI Yijun | 2019 | Acta Geologica Sinica(English Edition)2019，93，2： | 4 |
| 6 | A Recombinant Rabies Virus Expressing Fms-like Tyrosine Kinase 3 Ligand(Flt3L) Induces Enhanced Immunogenicity in Mice显示文摘Rabies is a zoonotic disease that still causes 59,000 human deaths each year,and rabies vaccine is the most effective way to control the disease.Our previous studies suggested that the maturation of DC plays an important role in enhancing the immunogenicity of rabies vaccine.Flt3L has been reported to own the ability to accelerate the DC maturation,therefore,in this study,a recombinant rabies virus expressing mouse Flt3L,designated as LBNSE-Flt3L,was constructed,and its immunogenicity was characterized.It was found that LBNSE-FU3L could enhance the maturation of DC both in vitro and in vivo,and significantly more TFH cells and Germinal Center B(GC B)cells were generated in mice immunized with LBNSE-FU3L than those immunized with the parent virus LBNSE.Consequently,expressing of Flt3L could elevate the level of virus-neutralizing antibodies(VNA)in immunized mice which provides a better protection from a lethal rabies virus challenge.Taken together,our study extends the potential of Flt3L as a good adjuvant to develop novel rabies vaccine by enhancing the VNA production through activating the DC—Tfh^GC B axis in immunized mice. | Yachun Zhang Jie Yang Mingming Li Min Cui Zhen FFu Ling Zhao Ming Zhou | 2019 | Virologica Sinica2019，34，6： | 3 |
| 7 | Innovation Requirements and Progress of Dialysis Water Treatment System显示文摘With the development of medicine,people are becoming more and more aware of the quality of medical water supply,such as dial ysis water,dental water,preparation water,operating room water,supply room water.Therefore,People are constantly improving the water treatment system and its standardized,and the demand for dialysis water is also becoming more and more strict.Hemodialysis is an effective means of maintaining the life of renal failure patients,120~180L water will be used for a conventional dialysis treatment,and high flux dialysis will consume more. | Jun Ke Lifong Yan Taksui Wong Bo Hu Shuang Cui Sibo Huang Aiyun Cha Jie Huang Hongwei Hu Dawen Yun Shaofeng Huang Jie Shu Mingming Ma Lianghong Yin Xiangnan Dong Chen Yun Zuhui Chen Fanna Liu | 2017 | 临床医学工程2017，24，S1： | 2 |
| 8 | Genetically modified CD7-targeting allogeneic CAR-T cell therapy with enhanced efficacy for relapsed/refractory CD7-positive hematological malignancies:a phase I clinical study显示文摘Chimeric antigen receptor(CAR)-T cell therapy against T cell malignancies faces major challenges including fratricide between CAR-T cells and product contamination from the blasts.Allogeneic CAR-T cells,generated from healthy donor T cells,can provide ready-to-use,blast-free therapeutic products,but their application could be complicated by graft-versus-host disease(GvHD)and host rejection.Here we developed healthy donor-derived,CD7-targeting CAR-T cells(RD13-01)with genetic modifications to resist fratricide,GvHD and allogeneic rejection,as well as to potentiate antitumor function.A phase I clinical trial(NCT04538599)was conducted with twelve patients recruited(eleven with T cell leukemia/lymphoma,and one with CD7-expressing acute myeloid leukemia).All patients achieved pre-set end points and eleven proceeded to efficacy evaluation.No dose-limiting toxicity,GvHD,immune effector cell-associated neurotoxicity or severe cytokine release syndrome(grade≥3)were observed.28 days post infusion,81.8%of patients(9/11)showed objective responses and the complete response rate was 63.6%(7/11,including the patient with AML).3 of the responding patients were bridged to allogeneic hematopoietic stem cell transplantation.With a median follow-up of 10.5 months,4 patients remained in complete remission.Cytomegalovirus(CMV)and/or Epstein-Barr virus(EBV)reactivation was observed in several patients,and one died from EBV-associated diffuse large B-cell lymphoma(DLBCL).Expansion of CD7-negative normal T cells was detected post infusion.In summary,we present the first report of a Phase I clinical trial using healthy donor-derived CD7-targeting allogeneic CAR-T cells to treat CD7^(+)hematological malignancies.Our results demonstrated the encouraging safety and efficacy profiles of the RD13-01 allogeneic CAR-T cells for CD7^(+)tumors. | Yongxian Hu Yali Zhou Mingming Zhang Houli Zhao Guoqing Wei Wengang Ge Qu Cui Qitian Mu Gong Chen Lu Han Tingting Guo Jiazhen Cui Xiaoyan Jiang Xiujun Zheng Shuhui Yu Xiaolong Li Xingwang Zhang Mingxi Chen Xiuju Li Ming Gao Kang Wang Cheng Zu Hao Zhang Xiaohong He Yanbin Wang Dongrui Wang Jiangtao Ren He Huang | 2022 | Cell Research2022，32，11： | 2 |
| 9 | Dual-site fluorescent probe for highly selective and sensitive detection of sulfite and biothiols显示文摘A dual-site fluorescent probe with double bond and aldehyde as reactive sites, was designed for the selective detection of sulfite and biothiols. Sulfite reacts with conjugate bond selectively, while Cys responses with aldehyde and GSH occurs substitution reaction. Different interactions cause different absorption and fluorescence responses. Moreover, it could be further applied in imaging in living cells. | Mengyang Li Pengcheng Cui Kun Li Jiahui Feng Mingming Zou Xiaoqi Yu | 2018 | Chinese Chemical Letters2018，29，6： | 1 |
| 10 | Protective effect of puerarin on diabetic retinopathy in rats显示文摘 | Yan Teng Hao Cui Mingming Yang Han Song Qingsheng Zhang Ying Su Jianqiu Zheng | 2009 | Molecular Biology Reports2009，，5： | 1 |
| 11 | Is there a great unconformity between Xiamaling and Longshan formations in the North China Craton?显示文摘The existence of a 0.3 Ga'great unconformity'between the Mesoproterozoic'Undefined System'Xiamaling Formation and the'Neoproterozoic'Longshan Formation has long been controversial.In this study,stratigraphy,sedimentology,detrital zircon dating,lithium isotope,and major and trace elements were applied to systematically analyze their relationship.Results demonstrate that coarse to fine sandstone-mudstone normal cycles with different grades and thicknesses exist from the Xiamaling to the Longshan formations.Sedimentary succession formed from a restricted platform with lagoon-shallow marine shelf-tidal flat-beach and tidal shoal developing in ascending order.The pebbly sandstone at the bottom of the Long shan Formation does not have the properties of basal conglomerate with tectonic significance.The youngest detrital zircon is older than 1.6 Ga for the Long shan Formation and overlying Jing'eryu Formation sandstones.In addition,no(zircon)provenance has been found from the large igneous province of 1.32-1.2 Ga in Xiamaling Formation.Theδ^(7)Li isotope values exhibit a gradually increasing trend.The Li content and CIA(Chemical index of alteration)gradually decreases from the top of the Xiamaling Formation to the lower part of the Long shan Formation.Simultaneously,as K_(2)O/Al_(2)O_(3)and FeO/Fe_(2)O_(3)decreases,Na_(2)O/Al_(2)O_(3)increases,and Li/Al,V/Cr,and V/(V+Ni)fluctuates slightly.At the boundary,the Ce/Ce^(*)and Eu/Eu^(*)show positive and negative anomalies,respectively.Further,the Rb/Sr and Al/Si values decrease,and U/Th values increase.This indicates that the geochemistry of the weathering crust at the interface of the Xiamaling Formation-Long shan Formation is not characteristic.However,it indicates a weakening of continental weathering intensity from the bottom to the top of the interface.These results do not support the existence of a large unconformity between the Xiamaling and the Longshan formations.Therefore,it is suggested that the Long shan Formation-Jing'eryu Formation should be moved to the lower level in the stratigraphic chart,where it should form part of the Mesoproterozoic'Undefined System'along with the Xiamaling Formation.On this basis,the paleogeography of the Xiamaling Formation-Longshan Formation-Jing'eryu Formation sedimentary period in the Yanliao area of North China has been reclassified. | Hongwei KUANG Nan PENG Yongqing LIU Yuchong WANG Mingming CUI Xiaoshuai CHEN Dawei QIAO Keining QI | 2023 | Science China Earth Sciences2023，66，5： | 1 |
| 12 | Key-Recovery Attacks on LED-Like Block Ciphers显示文摘Asymmetric cryptographic schemes, represe nted by RSA, have bee n show n to be in secure un der quantum computing conditions. Correspondingly, there is a need to study whether the symmetric cryptosystem can still guarantee high security with the advent of quantum computers. In this paper, based on the basic principles of classical slide attacks and Simon's algorithm, we take LED-like lightweight block ciphers as research objects to present a security analysis under both classical and quantum attacks, fully considering the influence on the security of the ciphers of adding the round constants. By analyzing the information leakage of round constants, we can introduce the differential of the round constants to propose a classical slide attack on full-round LED-64 with a probability of 1. The analysis result shows that LED-64 is unable to resist this kind of classical slide attack, but that attack method is not applicable to LED-128. As for quantum attacks, by improving on existing quantum attack methods we dem on strate a qua ntum single-key slide attack on LED-64 and a quantum related-key attack on LED- 128, and indicators of the two attack algorithms are analyzed in detail. The attack results show that adding round consta nts does not completely improve the security of the ciphers, and quantum attacks can provide an exp on ential speed-up over the same attacks in the classical model. It further illustrates that the block cipher that is proved to be safe under classical settings is not necessarily secure under quantum conditions. | Linhong Xu Jiansheng Guo Jingyi Cui Mingming Li | 2019 | Tsinghua Science and Technology2019，24，5： | 1 |
| 13 | CCGD-ESCC: A Comprehensive Database for Genetic Variants Associated with Esophageal Squamous Cell Carcinoma in Chinese Population显示文摘Esophageal squamous-cell carcinoma(ESCC) is one of the most lethal malignancies in the world and occurs at particularly higher frequency in China. While several genome-wide association studies(GWAS) of germline variants and whole-genome or whole-exome sequencing studies of somatic mutations in ESCC have been published, there is no comprehensive database publically available for this cancer. Here, we developed the Chinese Cancer Genomic Database-Esophageal Squamous Cell Carcinoma(CCGD-ESCC) database, which contains the associations of 69,593 single nucleotide polymorphisms(SNPs) with ESCC risk in 2022 cases and 2039 controls, survival time of 1006 ESCC patients(survival GWAS) and gene expression(expression quantitative trait loci,eQTL) in 94 ESCC patients. Moreover, this database also provides the associations between8833 somatic mutations and survival time in 675 ESCC patients. Our user-friendly database is a resource useful for biologists and oncologists not only in identifying the associations of genetic variants or somatic mutations with the development and progression of ESCC but also in studying the underlying mechanisms for tumorigenesis of the cancer. CCGD-ESCC is freely accessible at http://gffzzdf7d528e3a264cb9hq5bonwkno9cq6059.ffgz.tsg.suse.edu.cn/ccgd/ESCCdb. | Linna Peng Sijin Cheng Yuan Lin Qionghua Cui Yingying Luo Jiahui Chu Mingming Shao Wenyi Fan Yamei Chen Ai Lin Yiyi Xi Yanxia Sun Lei Zhang Chao Zhang Wen Tan Ge Gao Chen Wu Dongxin Lin | 2018 | Genomics, Proteomics & Bioinformatics2018，16，4： | 1 |
| 14 | DHD4,a CONSTANS-like family transcription factor,delays heading date by affecting the formation of the FAC complex in rice显示文摘Heading date(or flowering time)is one of the most important agronomic traits in rice,influencing its regional adaptability and crop yield.Many major-effect genes for rice heading date have been identified,but in practice they are difficult to be used for rice molecular breeding because of their dramatic effects on heading date.Genes with minor effects on heading date,which are more desirable for fine-tuning flowering time without significant yield penalty,were seldom reported.In this study,we identified a new minor-effect heading date repressor,Delayed Heading Date 4(DHD4).The dhd4 mutant shows a slightly earlier flowering phenotype without a notable yield penalty compared with wild-type plants under natural long-day conditions.DHD4 encodes a CONSTANS-like transcription factor localized in the nucleus.Molecular,biochemical,and genetic assays show that DHD4 can compete with 14-3-3 to interact with OsFD1,thus affecting the formation of the Hd3a-14-3-3-OsFD1 triprotein FAC complex,resulting in reduced expression of OsMADS14 and OsMADS15,and ultimately delaying flowering.Taken together,these results shed new light on the regulation of flowering time in rice and provide a promising target for fine-tuning flowering time to improve the regional adaptability of rice. | Maohong Cai Shanshan Zhu Mingming Wu Xiaoming Zheng Jiachang Wang Liang Zhou Tianhui Zheng Song Cui Shirong Zhou Chaonan Li Huan Zhang Juntao Chai Xinyue Zhang Xin Jin Zhijun Cheng Xin Zhang Cailin Lei Yulong Ren Qibing Lin Xiuping Guo Lei Zhao Jie Wang Zhichao Zhao Ling Jiang Haiyang Wang Jianmin Wan | 2021 | Molecular Plant2021，14，2： | 1 |
| 15 | Application Status and Prospect of Bio-artificial Liver显示文摘Liver failure which can be caused by viral hepatitis,alcohol,drugs,metabolic diseases,autoimmune processes or other fac tors is the end stage of chronic liver disease.Although liver transplantation is currently considered to be the primary treatment measures of chronic liver disease.Due to donor shortages,surgical complications and immune rejection,cell therapy has been extensively studied.?Hepa tocyte transplantation and artificial liver have evolved into a simpler alternative to liver failure treatment.Artificial liver can be used as Liver replacement therapy in patients who were waiting for the liver transplantation with chronic liver disease.The ideal biological artificial liver must have the liver material metabolism,detoxification,synthesis and secretion and other functions.Nowadays bio-artificial liver has carried out a large number of clinical trials and get some progress.?This article is now discuss the status of bio-artificial liver and its re placement therapy prospects. | Xiaoyi Chen Mingming Ma Sibo Huang Shuang Cui Huanhuan Liu Jun Ke Shengling Huang Xiangnan Dong Huixia Yu Yongpin Lu Chen Yun Yu Mong Fanna Liu B.Hocher Hongwei Hu Jie Shu Lianghong Yin | 2017 | 临床医学工程2017，24，S1： | 0 |
| 16 | Advances in Diagnosis and Treatment of Neuropsychiatric Systemic Lupus Erythematosus显示文摘1 Introduction Neuropsychiatric systemic lupus erythematosus(NPSLE)is a serious complication of systemic lupus erythematosus(SLE),with an incidence of about 30%to 40%[1].No matter early or late SLE patients are prone to concurrent,so early diagnosis and treatment of NPSLE is extremely important. | Shufei Zeng Yangyang Zang Dezhen Chen Baozhang Guan Fanna Liu Mingming Ma Yu Mong Huiyuan Zheng Aiyun Cha Xiangnan Dong Huanhuan Liu Taksui Wong Shuang Cui Tong Liu Yongpin Lu Chen Yun Hongwei Hu B.Hocher W.Pommer Zuhui Chen Lianghong Yin | 2017 | 临床医学工程2017，24，S1： | 0 |
| 17 | A novel optical high-pressure apparatus for studying the combustion of solid propellant under periodic strain显示文摘The combustion of solid propellants can provide tremendous thrust to drive solid rocket motors.Solid propellant grains are subjected to mechanical loads during practical combustion applications.The causes of mechanical loads include,but are not limited to,impulsive loads during ignition and periodic loads(>5 Hz)due to mechanical oscillation[1,2]. | OUYANG JianFeng GU MingMing WANG ShaoJie CUI CunHao SHI XiaoMing HOU KaiYu ZHOU ZhongYue QI Fei | 2023 | Science China(Technological Sciences)2023，66，3： | 0 |
| 18 | DAAPS: A Deformable-Attention-Based Anchor-Free Person Search Model显示文摘Person Search is a task involving pedestrian detection and person re-identification,aiming to retrieve person images matching a given objective attribute from a large-scale image library.The Person Search models need to understand and capture the detailed features and context information of smaller objects in the image more accurately and comprehensively.The current popular Person Search models,whether end-to-end or two-step,are based on anchor boxes.However,due to the limitations of the anchor itself,the model inevitably has some disadvantages,such as unbalance of positive and negative samples and redundant calculation,which will affect the performance of models.To address the problem of fine-grained understanding of target pedestrians in complex scenes and small sizes,this paper proposes a Deformable-Attention-based Anchor-free Person Search model(DAAPS).Fully Convolutional One-Stage(FCOS),as a classic Anchor-free detector,is chosen as the model’s infrastructure.The DAAPS model is the first to combine the Anchor-free Person Search model with Deformable Attention Mechanism,applied to guide the model adaptively adjust the perceptual.The Deformable Attention Mechanism is used to help the model focus on the critical information and effectively improve the poor accuracy caused by the absence of anchor boxes.The experiment proves the adaptability of the Attention mechanism to the Anchor-free model.Besides,with an improved ResNeXt+network frame,the DAAPS model selects the Triplet-based Online Instance Matching(TOIM)Loss function to achieve a more precise end-to-end Person Search task.Simulation experiments demonstrate that the proposed model has higher accuracy and better robustness than most Person Search models,reaching 95.0%of mean Average Precision(mAP)and 95.6%of Top-1 on the CUHK-SYSU dataset,48.6%of mAP and 84.7%of Top-1 on the Person Re-identification in the Wild(PRW)dataset,respectively. | Xiaoqi Xin Dezhi Han Mingming Cui | 2023 | Computers, Materials & Continua2023，77，11： | 0 |
| 19 | Puerarin inhibits NHE1 activity by interfering with the p38 pathway and attenuates mitochondrial damage induced by myocardial calcium overload in heart failure rats显示文摘Previous studies have shown that puerarin plays a key role in protecting humans and animals from cardiovascular diseases.The exact mechanism of the therapeutic effect of puerarin on various cardiovascular diseases(protective effect on cardiomyocytes)is still unclear.In the present study,we identify the role of puerarin in an animal model of experimental heart failure(HF)and explore its underlying mechanisms.The HF rat model is induced by in-traperitoneal injection of adriamycin(ADR),and puerarin is administered intragastrically at low,medium,and high concentrations.We demonstrate that puerarin significantly improves myocardial fibrosis and inflammatory in-filtration and,as a result,improves cardiac function in ADR-induced HF rats.Mechanistically,we find for the first time that puerarin inhibits overactivated Na+/H+exchange isoform 1(NHE1)in HF,which may improve HF by decreasing Na+and Ca2+ion concentrations and attenuating mitochondrial damage caused by calcium overload;on the other hand,puerarin inhibits the activation of the p38 pathway in HF,reduces the expressions of TGF-βand proinflammatory cytokines,and suppresses myocardial fibrosis.In conclusion,our results suggest that Puerarin is an effective drug against HF and may play a protective role in the myocardium by inhibiting the activation of p38 and its downstream NHE1. | Guopin Pan Baoyue Cui Mingming Han Laibiao Lin Yinlan Li Ling Wang Shuang Guo Yaling Yin Heqin Zhan Peng Li | 2024 | Acta Biochimica et Biophysica Sinica2024，56，2： | 0 |
| 20 | Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat∞显示文摘Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution,and is commonly employed in wheat breeding programs.Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination,but the underlying mechanism is poorly understood.Here,we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid(Triticum durum,AABB)and hexaploid wheat(Triticum aestivum,AABBDD).In the paternal‐versus the maternal‐excess cross,the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed,causing reduced germination percentage.The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types.Furthermore,expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses,leading to increased number of imprinted genes.The endosperm‐specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele‐specific DNA methylation.Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019,leading to reduced storage protein and starch accumulation during endosperm development in paternal‐excess cross,as confirmed by interploidy crosses between tetraploid wild‐type and clustered regularly interspaced palindromic repeats(CRISPR)–CRISPR‐associated protein 9 generated hexaploid mutants.These findings reveal a contribution of genomic imprinting to paternal‐excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal‐excess interploidy crosses in wheat breeding programs. | Guanghui Yang Man Feng Kuohai Yu Guangxian Cui Yan Zhou Lv Sun Lulu Gao Yumei Zhang Huiru Peng Yingyin Yao Zhaorong Hu Vincenzo Rossi Ive De Smet Zhongfu Ni Qixin Sun Mingming Xin | 2023 | Journal of Integrative Plant Biology2023，65，12： | 0 |
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