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| 1 | 贵州省安龙县狂犬病的流行病学研究显示文摘为了探讨局部地区暴发流行的因素,对2004年与2005年间在贵州省安龙县及其周边发生的人间狂犬病进行个案调查,用直接免疫荧光法检测犬脑组织中的狂犬病毒抗原,用RT-PCR法扩增G基因片段,测定核苷酸序列并构建系统发生树进行遗传特征分析。自1994-2003年安龙县报告无人间狂犬病疫情,但于2004与2005年分别报告44例与27例人狂犬病。71例狂犬病患者中68人被犬所伤,3人被猫所伤。在有完整记录的69例患者中,潜伏期平均为47.5d(10~282),13例狂犬病患者的潜伏期≤15d(18.84%);32例的潜伏期≤30d(46.38%),尤其是在2004年的患者中,潜伏期≤20d的病例有13例,占30%。2005年在疫点周围采集的85只犬脑组织中,5只犬脑组织狂犬病毒抗原阳性,阳性率为5.88%。A13株与A48株病毒之间的G基因核苷酸序列同源性为86.5%,与已报道病毒株比较发现,A13株与广西分离株有最高的同源性,而A48株却与印度尼西亚分离株SN01-23的同源性最高。比较分析两株病毒G基因编码的氨基酸序列,发现包括GⅠ、GⅡ,以及GⅢ抗原位点内的区间,A13与A48分别有19和28个氨基酸位点发生了氨基酸的替代。用全G基因核苷酸序列进行系统分析发现,两株毒株全为Ⅰ型狂犬病毒。结果表明引起安龙县的狂犬病流行的病毒不是新型狂犬病毒,犬饲养量大与病毒携带率高是狂犬病发病暴发流行的直接原因。 | 张永振 熊成龙 邹洋 王定明 余春 周敬祝 王昭孝 张永荣 Zhen F.FU | 2006 | 中国病毒学2006，21，4： | 34 |
| 2 | 狂犬病毒糖蛋白研究进展显示文摘 | 徐兰 郭增柱 张永振 熊成龙 Zhen F.Fu | 2006 | 中国人兽共患病学报2006，22，9： | 21 |
| 3 | 狂犬病毒生物学特征研究进展显示文摘狂犬病是由狂犬病毒(Rabies virus,RV)引起的,以恐水、畏光、吞咽困难、狂躁等临床表现为特征的致死性中枢神经系统感染疾病,一旦发病,病死率几乎达100%,是迄今为止人类病死率最高的急性传染病。随着分子生物学等相关学科的快速发展,RV的基因分型、临床诊断、新型RV的发现、演变进化,以及病毒的毒力及其致病性等研究取得了很大成就。本文就近年来RV生物学特征的研究进展作一综述。 | 熊成龙 张永振 卢思奇 Zhen F.Fu | 2006 | 中华流行病学杂志2006，27，10： | 18 |
| 4 | 汉坦病毒系统发生分析及其基因分型的研究显示文摘目的寻找适于汉坦病毒进行系统发生分析的系统发生树构建方法及其基因片段。选用GenBank中部分有代表性的汉坦病毒株,用M、S、M+S全序列,以及G1、G2的2003-2302、2741-2950核苷酸片段分别以邻位相连法(NJ)和最大简约法(MP)构建系统发生树。用M+S、M、G1、以及G2的2003-2302片段以NJ法构建的系统发生树将所选25株病毒分为5个分支,分别对应于HTN、SEO、DOB、PUU血清型及引起HPS的Sin Nombre血清族。但用G2区的2741-2950片段构建的系统发生树将DOB型与HTN型分为一支。以MP法用M、M+S及G1片段构建的发生树,结果与NJ法相同,但用S与G2的2003-2302、2741-2950片段构建的系统发生树则不能将所有的病毒株分在相应的血清型。因此,汉坦病毒的基因分型及系统发生分析时,NJ法优于MP法;用M片段的G1核苷酸序列可以替代M与S片段全序列进行基因分型及系统发生分析。 | 张永振 屈勇刚 杨东明 李莹 Zhen F.Fu | 2006 | 中国人兽共患病学报2006，22，3： | 7 |
| 5 | Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques显示文摘African swine fever(ASF)is an infectious disease caused by African swine fever virus(ASFV)with clinical symptoms of high fever,hemorrhages and high mortality rate,posing a threat to the global swine industry and food security.Quarantine and control of ASFV is crucial for preventing swine industry from ASFV infection.In this study,a recombinase polymerase amplification(RPA)-CRISPR-based nucleic acid detection method was developed for diagnosing ASF.As a highly sensitive method,RPA-CRISPR can detect even a single copy of ASFV plasmid and genomic DNA by determining fluorescence signal induced by collateral cleavage of CRISPR-lw Cas13 a(previously known as C2c2)through quantitative real-time PCR(q PCR)and has the same or even higher sensitivity than the traditional q PCR method.A lateral flow strip was developed and used in combination with RPA-CRISPR for ASFV detection with the same level of sensitivity of Taq Man q PCR.Likewise,RPA-CRISPR is capable of distinguishing ASFV genomic DNA from viral DNA/RNA of other porcine viruses without any cross-reactivity.This diagnostic method is also available for diagnosing ASFV clinical DNA samples with coincidence rate of 100%for both ASFV positive and negative samples.RPA-CRISPR has great potential for clinical quarantine of ASFV in swine industry and food security. | Meishen Ren Hong Mei Ming Zhou Zhen F.Fu Heyou Han Dingren Bi Fuhu Peng Ling Zhao | 2021 | Virologica Sinica2021，36，2： | 5 |
| 6 | Recombinant rabies virus expressing IL-15 enhances immunogenicity through promoting the activation of dendritic cells in mice显示文摘Rabies remains a public health threat that kills approximately 59,000 people worldwide each year,most of which are from the developing countries of Africa and Asia where dog rabies are endemic.Therefore, developing an affordable and efficacious vaccine is crucial for rabies control in these countries. Interleukin(IL)-15, an immunoregulatory cytokine, is a pluripotent molecule with therapeutic potential, which targets many cell types and links the innate and adaptive immune system. In this study, IL-15 gene was cloned and inserted into the genome of a recombinant rabies virus(RABV) strain LBNSE(designated as LBNSE-IL15), and the effect of over-expression of IL-15 on the immunogenicity of RABV was investigated. It was found that mice vaccinated with LBNSEIL15 could induce significantly higher level of virus-neutralizing antibody(VNA) than those immunized with LBNSE, resulting in the higher protection after challenge. Further investigation was performed to find out the possible role of IL-15 plays in the process of antibody induction, and it was found that LBNSE-IL15 could enhance the maturation of dendritic cells(DCs) in immunized mice. Furthermore, the mice immunized with LBNSE-IL15 could promote the T_(FH) cells differentiation and the generation of germinal center B cells and plasma cells. Together, these data indicated that IL-15 could be a potential adjuvant in enhancing the immunogenicity of RABV, contributing to the development of more-efficacious rabies vaccines. | Tiange Chen Yajing Zhang Zhao Wang Jie Yang Mingming Li Kunlun Wang Min Cui Zhen F.Fu Ling Zhao Ming Zhou | 2017 | Virologica Sinica2017，32，4： | 5 |
| 7 | Progress and Prospects of Dog-Mediated Rabies Elimination in China显示文摘CURRENT STATUS OF RABIES IN CHINA Through concerted stakeholder effort,rabies prevention and control in China has achieved remarkable results.The reported incidence of human rabies continues to decline at a rate of approximately 20%each year.In 2020,a total of 202 human rabies cases were reported countrywide,a decrease of 94%when compared with the most recent peak in 2007(3,300 human cases).Likewise,rabies was reported only in 143 counties and districts. | Wenwu Yin Zhen F.Fu George F.Gao | 2021 | China CDC weekly2021，3，39： | 1 |
| 8 | Launching Animal Diseases: animal warfare and health, food safety, and public health显示文摘It is indeed our great pleasure to have the inaugural issue of the Animal Diseases,an open access and fast peer-reviewed journal,published.Animal diseases not only cause enormous economic losses(more than 20%)to the animal farming industry,but also pose serious threats to human health as more than 60%human infectious diseases originate in animals.Therefore,it is absolutely imperative to conduct research on all aspects of animal diseases as well as to develop novel drugs,vaccines,diagnostic methods and technologies to address above challenges.To promote One Health initiative,Animal Diseases is committed to publish high quality manuscripts in animal disease research/application which are closely related to human health,including the latest state-of-the-art articles from academia,government laboratories and industry experts.The journal publishes original research,review,case report and other types of communications in all areas of animal diseases,infectious or noninfectious.It has partnered with BMC,part of SpringerNature,and a pioneer of open access publishing.Initially electronic publication will proceed as manuscripts are accepted. | Zhen F.Fu Huanchun Chen | 2021 | Animal Diseases2021，1，1： | 0 |
| 9 | Exhaustive Exercise Does Not Affect Humoral Immunity and Protection after Rabies Vaccination in a Mouse Model显示文摘Rabies is one of the most dangerous and widespread zoonosis and is characterized by severe neurological signs and a high case-mortality rate of nearly 100%. Vaccination is the most effective way to prevent rabies in humans and animals. In this study, the relationship between exhaustive exercise and the humoral immune response after immunization with inactivated rabies vaccine was investigated in a mouse model with one-time exhaustive exercise. It was found that compared with the mice with no exercise after vaccination, no significant differences were found in those with exhaustive exercise after vaccination on body-weight changes, virus-neutralizing antibody(VNA) titers, antibody subtypes and survivor ratio after lethal rabies virus(RABV) challenge. This study indicated that exhaustive exercise does not reduce the effects of the rabies inactivated vaccine. | Lun Xia Minrui Li Yajing Zhang Juncheng Ruan Jie Pei Jiale Shi Zhen F.Fu Ling Zhao Ming Zhou Dayong Tian | 2018 | Virologica Sinica2018，33，3： | 0 |
| 10 | Identification of tick-borne pathogens using metagenomic analyses in H.longicornis feeding on humans in downtown Beijing显示文摘On August 14th,2018,a Beijing resident living in Xicheng District found a female H.longicornis tick attached to the skin at the front of his upper shin.On examination,the patient was afebrile and appeared well.The species of the tick was identified through morphological characteristics and phylogenetic analysis based on cytochrome C oxidase subunit I.This H.longicornis tick was screened for tick-borne pathogens such as viruses,bacteria and parasites.RNA pathogens were screened by PCR and sequencing,while DNA pathogens were screened by metagenomic analyses.It was found that the tick was positive for the DNA sequences of zoonotic and animal pathogens such as A phagocytophilum,Ehrlichia minasensis and C.burnetii.Considering the good health condition of the patient,we hypothesized that the pathogens originated from the tick specimen itself rather than host blood meal.For the first time,our study reveals the possible risk of transmission of tick-borne pathogens to human beings through tick bit in downtown Beijing.Further research is needed to screen for tick-borne pathogens among unfed ticks collected from central Beijing. | Jizhou Lv Huiyu Wang Xueqing Han Lin Mei Xiangfen Yuan Yufang Kong Junhua Deng Zhen F.Fu Shaoqiang Wu Xiangmei Lin | 2021 | Animal Diseases2021，1，3： | 0 |
| 11 | Animal diseases and human future显示文摘Animals play an important role in our lives.Increased human-animal interactions lead to the transmission of zoonotic pathogens between animals and humans,directly threatening human health and societal development.Nearly all major public health events that occurred in the last several decades have been closely related to animal diseases.Approximately,60%of human infectious diseases are zoonotic,and 75%of emerging human infectious diseases originate from animals.As of April 20,2022,the global coronavirus infectious disease19(COVID-19)pandemic caused more than 503 million infections and more than 6.2 million deaths in humans.Over 11 billion doses of vaccines have been given to humans worldwide(WHO 2022).Despite the consensus among international organizations and animal experts that the SARS-CoV-2 originated from animals,the route of transmission between humans and other animals remains unclear.Furthermore,it is still a challenge for continually tracing the origin of SARS-CoV-2. | Min Cui Bang Shen Zhen F.Fu Huanchun Chen | 2022 | Animal Diseases2022，2，2： | 0 |
| 12 | Obituary:Professor Dr.Bernhard F.Dietzschold(1940–2022)显示文摘We share with great sadness that our long-time friend and colleague,Prof.Dr.Bernhard Dietzschold,passed away surrounded by his family on May 2,2022,at the age of 81.With the passing of Bernhard Dietzschold,the worldwide virology and infectious disease community is losing an eminent representative who has shaped the scientific landscape of its discipline for decades. | Jürgen A.Richt Zhen F.Fu | 2022 | Animal Diseases2022，2，3： | 0 |
| 13 | Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genomewide RNA Interference Screening显示文摘Mycobacterium tuberculosis is the causative agent of tuberculosis(TB), which is still the leading cause of mortality from a single infectious disease worldwide. The development of novel anti-TB drugs and vaccines is severely hampered by the complicated and time-consuming genetic manipulation techniques for M. tuberculosis. Here, we harnessed an endogenous type Ⅲ-A CRISPR/Cas10 system of M. tuberculosis for efficient gene editing and RNA interference(RNAi).This simple and easy method only needs to transform a single mini-CRISPR array plasmid, thus avoiding the introduction of exogenous protein and minimizing proteotoxicity. We demonstrated that M. tuberculosis genes can be efficiently and specifically knocked in/out by this system as confirmed by DNA high-throughput sequencing. This system was further applied to single-and multiple-gene RNAi. Moreover, we successfully performed genome-wide RNAi screening to identify M. tuberculosis genes regulating in vitro and intracellular growth. This system can be extensively used for exploring the functional genomics of M. tuberculosis and facilitate the development of novel anti-TB drugs and vaccines. | Khaista Rahman Muhammad Jamal Xi Chen Wei Zhou Bin Yang Yanyan Zou Weize Xu Yingying Lei Chengchao Wu Xiaojian Cao Rohit Tyagi Muhammad Ahsan Naeem Da Lin Zeshan Habib Nan Peng Zhen F.Fu Gang Cao | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 0 |
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