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| 1 | DDIT4 promotes gastric cancer proliferation and tumorigenesis through the p53 and MAPK pathways显示文摘Background:Gastric cancer(GC)is one of the most common malignancies worldwide,particularly in China.DNA damage-inducible transcript 4(DDIT4)is a mammalian target of rapamycin inhibitor and is induced by various cellular stresses;however,its critical role in GC remains poorly understood.The present study aimed to investigate the poten-tial relationship and the underlying mechanism between DDIT4 and GC development.Methods:We used western blotting,real-time polymerase chain reaction,and immunohistochemical or immunoflu-orescence to determine DDIT4 expression in GC cells and tissues.High-content screening,cell counting kit-8 assays,colony formation,and in vivo tumorigenesis assays were performed to evaluate cell proliferation.Flow cytometry was used to investigate cell apoptosis and cell cycle distribution.Results:DDIT4 was upregulated in GC cells and tissue.Furthermore,downregulating DDIT4 in GC cells inhibited proliferation both in vitro and in vivo and increased 5-fluorouracil-induced apoptosis and cell cycle arrest.In contrast,ectopic expression of DDIT4 in normal gastric epithelial cells promoted proliferation and attenuated chemosensitivity.Further analysis indicated that the mitogen-activated protein kinase and p53 signaling pathways were involved in the suppression of proliferation,and increased chemosensitivity upon DDIT4 downregulation.Conclusion:DDIT4 promotes GC proliferation and tumorigenesis,providing new insights into the role of DDIT4 in the tumorigenesis of human GC. | Feng Du Lina Sun Yi Chu Tingyu Li Chao Lei Xin Wang Mingzuo Jiang Yali Min Yuanyuan Lu Xiaodi Zhao Yongzhan Nie Daiming Fan | 2018 | Cancer Communications2018，38，1： | 10 |
| 2 | Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells显示文摘Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy(CADASIL)is a rare hereditary cerebrovascular disease caused by a NOTCH3 mutation.However,the underlying cellular and molecular mechanisms remain unidentified.Here,we generated non-integrative induced pluripotent stem cells(iPSCs)from fibroblasts of a CADASIL patient harboring a heterozygous NOTCH3 mutation(c.3226C>T,p.R1076C).Vascular smooth muscle cells(VSMCs)differentiated from CADASIL-specific iPSCs showed gene expression changes associated with disease phenotypes,including activation of the NOTCH and NF-kB signaling pathway,cytoskeleton disorganization,and excessive cell proliferation.In comparison,these abnormalities were not observed in vascular endothelial cells(VECs)derived from the patients iPSCs.Importantly,the abnormal upregulation of NF-kB target genes in CADASIL VSMCs was diminished by a NOTCH pathway inhibitor,providing a potential therapeutic strategy for CADASIL.Overall,using this iPSCbased disease model,our study identified clues for studying the pathogenic mechanisms of CADASIL and developing treatment strategies for this disease. | Chen Ling Zunpeng Liu Moshi Song Weiqi Zhang Si Wang Xiaoqian Liu Shuai Ma Shuhui Sun Lina Fu Qun Chu Juan Carlos Izpisua Belmonte Zhaoxia Wang Jing Qu Yun Yuan Guang-Hui Liu | 2019 | Protein & Cell2019，10，4： | 7 |
| 3 | Chromosome-level genome of Himalayan yew provides insights into the origin and evolution of the paclitaxel biosynthetic pathway显示文摘Taxus,commonly known as yew,is a well-known gymnosperm with great ornamental and medicinal value.In this study,by assembling a chromosome-level genome of the Himalayan yew(Taxus wallichiana)with 10.9 Gb in 12 chromosomes,we revealed that tandem duplication acts as the driving force of gene family evolution in the yew genome,resulting in the main genes for paclitaxel biosynthesis,i.e.those encoding the taxadiene synthase,P450s,and transferases,being clustered on the same chromosome.The tandem duplication may also provide genetic resources for the nature to sculpt the core structure of taxoids at different positions and subsequently establish the complex pathway of paclitaxel by neofunctionalization.Furthermore,we confirmed that there are two genes in the cluster encoding isoenzymes of a known enzyme in the paclitaxel biosynthetic pathway.The reference genome of the Himalayan yew will serve as a platform for decoding the complete biosynthetic pathway of paclitaxel and understanding the chemodi-versity of taxoids in gymnosperms. | Jian Cheng Xiao Wang Xiaonan Liu Xiaoxi Zhu Zihe Li Huanyu Chu Qian Wang QianQian Lou Bijun Cai Yiqun Yang Xiaoyun Lu Kai Peng Dingyu Liu Yuwan Liu Lina Lu Huan Liu Ting Yang Qijin Ge Chengcheng Shi Guichun Liu Zhiwei Dong Xun Xu Wen Wang Huifeng Jiang Yanhe Ma | 2021 | Molecular Plant2021，14，7： | 6 |
| 4 | A Local Cluster of Omicron Variant COVID-19 Likely Caused by Internationally Mailed Document——Beijing Municipality,China,January 2022显示文摘On January 15,2022,a 26 years old female(Case A)went to a sampling site for coronavirus disease 2019(COVID-19)testing due to excessive fatigue and fever for two days.This patient preliminarily tested positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)using a quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR)method. | Daitao Zhang Shuangsheng Wu Zhenyong Ren Ying Sun Xiangfeng Dou Zhaomin Feng Fu Li Yi Tian Shujuan Cui Baiwei Liu Bing Lyu Xiaoli Wang Feng Liu Lina Jin Chu Jiang Lina Tian Zhenjiang Xin Zhichao Liang Lei Jia Quanyi Wang Yang Pan Peng Yang | 2022 | China CDC weekly2022，4，14： | 6 |
| 5 | Decomposition of acid dissolved titanium slag from Australia by sodium hydroxide显示文摘The kinetics of the decomposition of acid dissolved titanium slag with a sodium hydroxide system under atmospheric pressure was studied. The effect of reaction temperature, particle size and NaOH-to-slag mass ratio on titanium extraction was investigated. The results show that temperature and particle size have significant influence on titanium extraction. The experimental data of titanium extraction show that the shrinking core model with chemical reaction controlled process is most applicable for the decomposition of slag, with an apparent activation energy of 62.4 kJ·mol-1. Approximately 85 wt.%-90 wt.% of the titanium can be extracted from the slag under the optimal conditions. In addition, the purity of titanium dioxide obtained in the product is up to 98.5 wt.%. | FENG Yang WANG Jinggang WANG Lina QI Tao XUE Tianyan CHU Jinglong | 2009 | Rare Metals2009，28，6： | 5 |
| 6 | Primed macrophages directly and specifically reject allografts显示文摘Monocytes and macrophages have long been associated with acute and chronic allograft rejection;this is mediated by their abilities to promote inflammation,kill target cells via antibody-dependent cytotoxicity and modulate adaptive immunity.Our present study showed that allogeneic antigen-primed macrophages acutely rejected skin grafts with specificity after adoptive transfer into MHC-matched immunodeficient mice.The ability of primed macrophages to reject allografts essentially requires the help of CD4^(+) T cells and does not require the help of CD8^(+) T cells.Moreover,the primed,perforin-deficient macrophages rejected the skin grafts in a significantly delayed pattern compared with WT macrophages,indicating that the perforin pathway of the primed macrophages is likely involved in the rejection process.Thus,primed macrophages are endowed with adaptive immunitylike features,such as specificity,with the help of CD4^(+) T cells during the immune response to allografts.The present study challenges our traditional views of macrophage functions and highlights the biological functions of macrophages beyond innate immunity in mammals. | Zhulang Chu Chenming Sun Lina Sun Chang Feng Fan Yang Yanan Xu Yong Zhao | 2020 | Cellular & Molecular Immunology2020，17，3： | 4 |
| 7 | A complete genome assembly of Glaciecola mesophila sp. nov. sequenced by using BIGIS-4 sequencer system显示文摘Using a pyrosequencing-based custom-made sequencer BIGIS-4, we sequenced a Gram-negative bacterium Glaciecola mesophila sp. nov. (Gmn) isolated from marine invertebrate specimens. We generated 152043 sequencing reads with a mean high-quality length of 406 bp, and assembled them using the BIGIS-4 post-processing module. No systematic low-quality data was detected beyond expected homopolymer-derived errors. The assembled Gmn genome is 5144318 bp in length and harbors 4303 annotated genes. A large number of metabolic genes correspond to various nutrients from surface marine invertebrates. Its abundant cold-tolerant and cellular signaling and related genes reveal a fundamental adaptation to low-temperature marine environment. | YUAN LiNa REN LuFeng LI YunTao HAN WeiJing YU Yong CHU YaNan LIU GuiMing YU Dan TENG MingJing WANG Liang WANG XuMin ZHOU XiaoGuang YU YuDe YU Jun | 2011 | Science China(Life Sciences)2011，54，9： | 4 |
| 8 | Synthesis and characterization of oriented linked LiFePO4 nanoparticles with fast electron and ion transport for high-power lithium-ion batteries显示文摘有不同形态学和尺寸的 LiFePO 4 nanoparticles 作为表面活化剂用环境地良性、便宜的甘油经由一个 solvothermal 方法被综合。粒子的形态学,尺寸,和结构被发现仔细联系到甘油的集中。面向非主要连接了 LiFePO 4 nanorods --[010 ] 与甘油的合适的集中被获得。nanorods 显示出好电子、离子的传导性,导致优异的率能力和骑车的性能。这表演非主要被归因于面向的连接[010 ] ,小粒子尺寸沿着[010 ] ,并且在 Fe 地点的李的职业。162.4 | Yi Jiang Ruiyuan Tian Haiqiang Liu Jiankun Chen Xinghua Tan Lina Zhang Guangyao Liu Hanfu Wang Lianfeng Sun Weiguo Chu | 2015 | Nano Research2015，8，12： | 4 |
| 9 | Axial gradient excitation accelerates volumetric imaging of two-photon microscopy显示文摘Two-photon excitation fluorescence microscopy(TPM),owing to its capacity for subcellular resolution,intrinsic optical sectioning,and superior penetration depth in turbid samples,has revolutionized biomedical research.However,its layer-by-layer scanning to form a three-dimensional image inherently limits the volumetric imaging speed and increases phototoxicity significantly.In this study,we develop a gradient excitation technique to accelerate TPM volumetric imaging.The axial positions of the fluorophores can be decoded from the intensity ratio of the paired images obtained by sequentially exciting the specimen with two axially elongated two-photon beams of complementary gradient intensities.We achieved a 0.63μm axial localization precision and demonstrate the flexibility of the gradient TPM on various sparsely labeled samples,including bead phantoms,mouse brain tissues,and live macrophages.Compared with traditional TPM,our technique improves volumetric imaging speed(by at least sixfold),decreases photobleaching(i.e.,less than 2.07±2.89%in 25 min),and minimizes photodamages. | Yufeng Gao Xianyuan Xia Lina Liu Ting Wu Tingai Chen Jia Yu Zhili Xu Liang Wang Fei Yan Zhuo Du Jun Chu Yang Zhan Bo Peng Hui Li Wei Zheng | 2022 | Photonics Research2022，10，3： | 3 |
| 10 | Synthesis of Core-Shell HZSM-5@SBA-15 Composite and Its Performance in the Conversion of Methanol to Aromatics显示文摘A core-shell HZSM-5@SBA-15 composite was prepared by the acidic sol-gel coating method using Pluronic P123 as the template. Its structure, properties, and catalytic performance in the conversion of methanol to aromatics(MTA) were compared with HZSM-5@SiO_2. The silica shells of these two samples can both reduce the acid sites of HZSM-5, and consequently can decrease the formation of coke on the catalyst. However, in the ordered mesoporous SBA-15 shell the aromatics and the coke precursors could diffuse more effectively than in the amorphous SiO_2 shell; therefore, in the MTA reaction catalyzed by HZSM-5@SBA-15 composite a higher BTX selectivity and a longer catalyst cycle length were observed. The cycle length of HZSM-5@SBA-15 was by 50 h longer than that of HZSM-5@SiO_2, and under this situation the selectivity of BTX still reached 56%. | Wang Lu Li Jian Wang Lijuan Chu Shuang Yang Lina | 2018 | China Petroleum Processing & Petrochemical Technology2018，20，1： | 2 |
| 11 | State estimation for multi-channel stochastic singular systems with multiplicative noise 显示文摘 | Chu Dongsheng Gao Shouwan Guo Lina | 2010 | Asian Journal of Control2010，12，6： | 1 |
| 12 | Identification of urinary biomarkers for type 2 diabetes using bead-based proteomic approach显示文摘 | Lina Chu Guangzhen Fu Qian Meng Hui Zhou Man Zhang | 2013 | Diabetes Research and Clinical Practice2013，，： | 1 |
| 13 | Decomposition kinetics of titanium slag in sodium hydroxide system 显示文摘 | Xue Tianyan Wang Lina Qi Tao Chu Jinglong Qu Jingkui Liu Changhou | 2009 | Hydrometallurgy2009，95，： | 1 |
| 14 | Decomposition kinetics of titanium slag in sodium hydroxide system显示文摘 | Tianyan Xue Lina Wang Tao Qi Jinglong Chu Jingkui Qu Changhou Liu | 2008 | Hydrometallurgy2008，，1： | 1 |
| 15 | Osteopontin Contributes to TGF-β1 Mediated Hepatic Stellate Cell Activation显示文摘 | Xiao Xiao Yi Gang Yong Gu Lina Zhao Jindong Chu Jinfeng Zhou Xiqiang Cai Hui Zhang Li Xu Yongzhan Nie Kaichun Wu Zhiguo Liu Daiming Fan | 2012 | Digestive Diseases and Sciences2012，，11： | 1 |
| 16 | DDIT4通过p53和MAPK通路促进胃癌增殖和肿瘤发生显示文摘背景与目的胃癌(gastric cancer,GC)是世界上最常见的恶性肿瘤之一,在我国尤为常见。DNA损伤诱导转录因子4(DNAdamage-inducibletranscript4,DDIT4)是哺乳动物雷帕霉素靶蛋白抑制因子,由多种细胞应激诱导产生;然而,它在胃癌中的关键作用仍然未知。本研究旨在探讨DDIT4与胃癌发展的关系及其作用机制。方法我们采用蛋白免疫印迹法、实时聚合酶链反应、免疫组化或免疫荧光法检测DDIT4在胃癌细胞和组织中的表达情况。采用高通量筛选、细胞计数试剂盒-8、克隆形成和体内成瘤实验检测细胞增殖情况。采用流式细胞术检测细胞凋亡和细胞周期分布情况。结果 DDIT4在胃癌细胞和组织中表达上调。在胃癌细胞中下调DDIT4表达可抑制体外和体内的细胞增殖并增强了5-氟尿嘧啶诱导的细胞凋亡和细胞周期阻滞。与之相反,在正常胃上皮细胞中DDIT4的异位表达促进了细胞增殖、降低了化疗敏感性。进一步研究表明,丝裂原活化蛋白激酶和p53信号通路参与抑制细胞增殖并在DDIT4下调后增强化疗敏感性。结论 DDIT4促进了胃癌细胞增殖和肿瘤发生,为研究DDIT4在人类胃癌发生、发展中的作用提供了新思路。 | Feng Du Lina Sun Yi Chu Tingyu Li Chao Lei Xin Wang Mingzuo Jiang Yali Min Yuanyuan Lu Xiaodi Zhao Yongzhan Nie Daiming Fan | 2019 | 癌症2019，38，3： | 1 |
| 17 | Entropy-driven atomic activation in supercooled liquids and its link to the fragile-to-strong transition显示文摘As a common but critical dynamic crossover in glass-forming liquids(GFLs),the discovery of fragile-to-strong(F-S)transition promises a novel route for understanding supercooled liquid and glass transition.The present work,for the first time,successfully realizes the quantitative prediction of the F-S transition in nine metallic glass-forming liquids,by a counter-intuitive approach that focuses on local atomic activation events,rather than relaxation,upon cooling.The dynamic crossover originates from a disorder-to-order transition by self-regulating behavior of atomic position within a cage controlled by finite atomic activation events,due to the appearance of local cooperative motion of nearest neighborhood atoms.Moreover,the dominant role of entropy in this anomaly has been discovered,and the correspondence between the crossover of configuration entropy involved in activation events and the occurrence of F-S transition has been found.Our work implies that the feature of atomic energy fluctuations reflected by atomic activation events has a close linkage to complex dynamic behaviors of disordered systems. | Wei Chu Zheng Wang Nannan Ren Bangshao Dong Jinhua Yu Pengfei Guan Yanhui Liu Yuanzheng Yue Lina Hu | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，4： | 0 |
| 18 | Barley leaves mediated biosynthesis of Au nanomaterials as a potential contrast agent for computed tomography imaging显示文摘Over the past few years, environment-friendly green synthetic methods for metal nanoparticles(NPs) have received considerable attention. The use of plant extracts is considered as potential for the biosynthesis of metal NPs due to its easier obtainment, cheap accessibility to industrial scale-up and high biosecurity. Herein, with the aqueous leaf extract of barley leaves as the template, we reported a rapid, single step, cost-effective and eco-friendly synthetic route to prepare new gold nanoparticles(BL-Au NPs).Significantly, except for the key role as the reducing, barley leaves was further utilized as the capping agent that ensure the stability of the BL-Au NPs. The characterization results of TEM, FTIR and UV-vis indicated that the gold nanoparticles coated with barley leaves extract were successfully synthesized. In vitro and zebra fish toxicity and stability assays demonstrated its good biocompatibility and high colloidal stability. The CT images in vitro demonstrated that the constructed BL-Au NPs possessed superiority in X-ray attenuation ability compared to clinically commonly used iodinated small molecular contrast agent. And BL-Au NPs can be used as contrast agent for effective CT imaging of zebra fish model. These results suggest the potential utility of BL-Au NPs as a CT imaging contrast agent in clinical CT imaging based diagnosis. | XUE NiChang ZHOU ChaoHui CHU ZhongYun CHEN LiNa JIA NengQin | 2021 | Science China(Technological Sciences)2021，64，2： | 0 |
| 19 | Role of Intracellular Distribution of Feline and Bovine SAMHD1 Proteins in Lentiviral Restriction显示文摘Human SAMHD1(h SAM)restricts lentiviruses at the reverse transcription step through its d NTP triphosphohydrolase(d NTPase)activity.Besides humans,several mammalian species such as cats and cows that carry their own lentiviruses also express SAMHD1.However,the intracellular distribution of feline and bovine SAMHD1(f SAM and b SAM)and its significance in their lentiviral restriction function is not known.Here,we demonstrated that f SAM and b SAM were both predominantly localized to the nucleus and nuclear localization signal(11KRPR14)-deleted f SAM and b SAM relocalized to the cytoplasm.Both cytoplasmic f SAM and b SAM retained the antiviral function against different lentiviruses and cytoplasmic f SAM could restrict Vpx-encoding SIV and HIV-2 more efficiently than its wild-type(WT)protein as cytoplasmic h SAM.Further investigation revealed that cytoplasmic f SAM was resistant to Vpx-induced degradation like cytoplasmic h SAM,while cytoplasmic b SAM was not,but they all demonstrated the same in vitro d NTPase activity and all could interact with Vpx as their WT proteins,indicating that cytoplasmic h SAM and f SAM can suppress more SIV and HIV-2 by being less sensitive to Vpx-mediated degradation.Our results suggested that f SAM-and b SAM-mediated lentiviral restriction does not require their nuclear localization and that f SAM shares more common features with h SAM.These findings may provide insights for the establishment of alternative animal models to study SAMHD1 in vivo. | Chu Wang Lina Meng Jialin Wang Kaikai Zhang Sizhu Duan Pengyu Ren Yingzhe Wei Xinyu Fu Bin Yu Jiaxin Wu Xianghui Yu | 2021 | Virologica Sinica2021，36，5： | 0 |
| 20 | Unveiling the Contribution of Lactic Acid to the Passivation Behavior of Ti-6Al-4V Fabricated by Laser Powder Bed Fusion in Hank’s Solution显示文摘In actual physiological environments,bacteria can activate the immune system and release lactic acid.However,the detailed contribution of lactic acid to the passivation behavior of titanium(Ti)alloys is still unclear.The current work investigated the in vitro passivation behavior of Ti-6Al-4V(TC4)alloys fabricated by laser powder bed fusion in Hank's solution with and without adding lactic acid.Electrochemical methods,inductively coupled plasma atomic emission spectrometer,and X-ray photoelectron spectroscopy were jointly used.Adding lactic acid decreases the corrosion resistance of samples by degrading the formed passive film.The film formed in the(lactic acid)-containing solution exhibits a higher level of oxygen vacancies and a lower thickness,attributed to the suppressed formation of Ti^(4+)transformed from Ti^(3+)and Ti^(2+).Moreover,the presence of lactic acid would increase the open circuit potential,relieve the ions release,and hinder the deposition of calcium phosphates within 24 h immersion. | Yu-Hang Chu Liang-Yu Chen Bo-Yuan Qin Wenbin Gao Fanmin Shang Hong-Yu Yang Lina Zhang Peng Qin Lai-Chang Zhang | 2024 | Acta Metallurgica Sinica(English Letters)2024，37，1： | 0 |
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