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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Instructional design and technology grounded in Enactivism A para- digm shift? 显示文摘 | Qing Li Bruce Clark and Ian Winchester | 2010 | British Journal of Educational Tech nology2010，3，41： | 1 |
| 3 | Improving bleachability of radiata pine kraft pulps following treatment with hemicellulolytic enzymes显示文摘 | Clark T A Steward D Bruce M E | 1991 | Appita1991，44，6： | 1 |
| 4 | Hypogastric artery ligation for obstetric hemorrhage显示文摘 | Clark SL Phelan JP Bruce SR | 1985 | Obstet Gynecol1985，66，： | 1 |
| 5 | Link- ing form and function: towards an eco- hydromor- phic ap -proach to sustainable river restoration 显示文摘 | Clarke S J Bruce - Burgess C L Wharton G | 2003 | Aquatic Conservation: Marine and Freshwater Eco- systems2003，，13： | 1 |
| 6 | Improving bleachability of radiata pine kraft pulps foilowing treatment with hemicellulolytic enzymes显示文摘 | Clark T A Steward D Bruce M E | 1991 | Appita J1991，44，6： | 1 |
| 7 | Improved bleachability of radiata pine kraft pulps following treatment with hemicelluloytic enzymes显示文摘 | Clark T A Steward D Bruce M E | 1991 | Appita1991，44，6： | 1 |
| 8 | The Relationship of Kolb Learning Styles, Online Learning Behaviors and Learning Outcomes显示文摘 | Hong Lu Lei Jia Shu-hong Gong Bruce Clark | 2007 | Educational Technology & Society2007，10，4： | 1 |
| 9 | The Rela- tionship of Kolb Learning Styles, Online Learning Behaviors and Learning Outcomes 显示文摘 | Hong Lu Lei Jia Shu -hong Gong and Bruce Clark | 2007 | Educational Technology & Society2007，10，4： | 1 |
| 10 | In vitro dissolution of sparingly water-soluble drug dosage forms显示文摘 | Vinod P. Shah Assad Noory Carol Noory Bruce McCullough Sanford Clarke Richard Everett Helen Naviasky B.N. Srinivasan Don Fortman Jerome P. Skelly | 1995 | International Journal of Pharmaceutics1995，，1： | 1 |
| 11 | Instructional Design and Technology Grounded in Enactivism:A Paradigm Shift? 显示文摘 | Qing Li Bruce Clark lan Winchester | 2010 | British Journal of Educational Technology2010，41，3： | 1 |
| 12 | Implement lateral position accuracy under RTK-GPS tractor guidance显示文摘 | Samuel Gan-Mor Rex L Clark Bruce L Upchurch | 2007 | Computers and Electronics in Agriculture2007，59，1： | 1 |
| 13 | Besifloxacin ophthalmic suspension 0.6% in the treatment of bacterial conjunctivitis patients with Pseudomonas aeruginosa infections显示文摘 | Laura Clark Heleen DeCory Timothy Comstock Lynne Gearinger Timothy Morris Bruce Silverstein | 2012 | Clinical Ophthalmology (default)2012，，： | 1 |
| 14 | Entropy and MDL discretization of continous variable for Bayesian belief networks显示文摘 | Ellis J Clarke Bruce A Barton | 2000 | International Journal of Intelligent Systems2000，15，1： | 1 |
| 15 | Total synthesis of the 5-HT3 receptor antagonits palonosetron 显示文摘 | BRUCE A KOWALCZYK CLARK A | 1996 | Synthesis1996，，7： | 1 |
| 16 | Meta-analysis of soy intake and breast cancer risk显示文摘 | Trock Bruce J Hilakivi Clarke Leena | 2006 | J Nail Cancer Inst2006，7，： | 1 |
| 17 | Radiofrequency Ablation of Small Renal Cell Carcinomas Using Multitined Expandable Electrodes: Preliminary Experience显示文摘 | Timothy W.I. Clark Bruce Malkowicz S. William Stavropoulos Richard Sanchez Michael C. Soulen Maxim Itkin Aalpen Patel Jeffrey I. Mondschein A.J. Wein | 2006 | Journal of Vascular and Interventional Radiology2006，，3： | 1 |
| 18 | Genetic analysis of opaque2 modifier loci in quality protein maize显示文摘 | David R. Holding Brenda G. Hunter Taijoon Chung Bryan C. Gibbon Clark F. Ford Arvind K. Bharti Joachim Messing Bruce R. Hamaker Brian A. Larkins | 2008 | Theoretical and Applied Genetics2008，，2： | 1 |
| 19 | The prevalence and incidence of biopsy-proven lupus nephritis in the UK显示文摘 | Patel M Clarke AM Bruce IN | 2006 | Arthritis Rheum2006，54，9： | 1 |
| 20 | Marketing Performance Measures: History and Interrelationship's显示文摘 | Clark Bruce H | 1999 | Journal of Marketing Management1999，15，： | 1 |
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