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| 1 | Efficacy and safety of tenofovir in chronic hepatitis B: Australian real world experience显示文摘AIM To evaluate the long-term treatment outcomes of tenofovir therapy in patients in a real world Australian tertiary care setting.METHODS We performed a retrospective analysis of treatment outcomes among treatment-na?ve and treatment-experienced patients receiving a minimum 3 mo tenofovir therapy through St Vincent's Hospital Melbourne, Australia. We included patients receiving tenofovir [tenofovir disoproxil fumarate(TDF)] monotherapy, as well as patients treated with TDF in combination with a second antiviral agent. Patients were excluded if they demonstrated human immune-deficiency virus/hepatitis C virus/hepatitis delta virus coinfection or were less than 18 years of age. We considered virological and biochemicalresponse, as well as safety outcomes. Virological response was determined by measurement of hepatitis B virus(HBV) DNA using sensitive assays; biochemical response was determined via serum liver function tests; histological response was determined from liver biopsy and fibroscan; safety analysis focused on glomerular renal function and bone mineral density. The primary efficacy endpoint was complete virological suppression over time, defined by HBV DNA < 20 IU/m L. Secondary efficacy endpoints included rates of biochemical response, and HB e antigen(HBe Ag)/HB surface antigen loss and seroconversion over time.RESULTS Ninety-two patients were identified who fulfilled the enrolment criteria. Median follow-up was 26 mo(range 3-114). Mean age was 46(24-78) years, 64(70%) were male and 77(84%) were of Asian origin. 55(60%) patients were treatment-na?ve and 62 patients(67%) were HBe Ag-negative. Complete virological suppression was achieved by 45/65(71%) patients at 12 mo, 37/46(80%) at 24 mo and 25/28(89%) at 36 mo. Partial virological response(HBV DNA 20-2000 IU/m L) was achieved by 89/92(96.7%) of patients. Multivariate analysis showed a significant relationship between virological suppression at end of follow-up and baseline HBV DNA level(OR = 0.897, 95%CI: 0.833-0.967, P = 0.0046) and HBe Ag positive status(OR = 0.373, 95%CI: 0.183-0.762, P = 0.0069). There was no difference in response comparing treatment-na?ve and treatment-experienced patients. Three episodes of virological breakthrough occurred in the setting of noncompliance. Tenofovir therapy was well tolerated.CONCLUSION Tenofovir is an efficacious, safe and well-tolerated treatment in an Australian real-world tertiary care setting. Our data are similar to the reported experience from registration trials. | Grace C Lovett Tin Nguyen David M Iser Jacinta A Holmes Robert Chen Barbara Demediuk Gideon Shaw Sally J Bell Paul V Desmond Alexander J Thompson | 2017 | World Journal of Hepatology2017，9，1： | 7 |
| 2 | Role of E3 ubiquitin ligases in lung cancer显示文摘E3 ubiquitin ligases are a large family of proteins that catalyze the ubiquitination of many protein substrates for targeted degradation by the 26S proteasome.Therefore,E3 ubiquitin ligases play an essential role in a variety of biological processes including cell cycle regulation,proliferation and apoptosis.E3 ubiquitin ligases are often found overexpressed in human cancers,including lung cancer,and their deregulation has been shown to contribute to cancer development.However,the lack of specific inhibitors in clinical trials is a major issue in targeting E3 ubiquitin ligases with currently only one E3 ubiquitin ligase inhibitor being tested in the clinical setting.In this review,we focus on E3 ubiquitin ligases that have been found deregulated in lung cancer.Furthermore,we discuss the processes in which they are involved and evaluate them as potential anti-cancer targets.By better understanding the mechanisms by which E3 ubiquitin ligases regulate biological processes and their exact role in carcinogenesis,we can improve the development of specific E3 ubiquitin ligase inhibitors and pave the way for novel treatment strategies for cancer patients. | Barbara C Snoek Leonie HAM de Wilt Gerrit Jansen Godefridus J Peters | 2013 | World Journal of Clinical Oncology2013，4，3： | 5 |
| 3 | Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase III study: 5-year analysis of the EORTC-NCIC trial显示文摘 | Roger Stupp Monika E Hegi Warren P Mason Martin J van den Bent Martin JB Taphoorn Robert C Janzer Samuel K Ludwin Anouk Allgeier Barbara Fisher Karl Belanger Peter Hau Alba A Brandes Johanna Gijtenbeek Christine Marosi Charles J Vecht Karima Mokhtari Piet | 2009 | Lancet Oncology2009，，5： | 4 |
| 4 | An in vitro prototype of a porcine biomimetic testis-like cell culture system: a novel tool for the study of reassembled Sertoli and Leydig cells显示文摘目前,有在 vitro 不可靠像睾丸的 biomimetic 机关文化系统设计了在 Sertoli 和 Leydig 房间上估计人的 gonadotropins 的功能的效果的装配 prepubertal。精子发生被调整由内分泌, paracrine,和 juxtacrine 因素(阴囊的串音) ,主要由象 luteinizing 荷尔蒙(LH ) 和由分别地刺激 Leydig 和 Sertoli 房间起一个枢轴的作用的刺激滤泡的荷尔蒙(FSH ) 那样的 gonadotropins 安排了。我们的学习的目的是建立一在里面 vitro prepubertal 是的猪的 bioengineered 构造为重新集合的 Sertoli 和 Leydig 房间上的试验性的研究的一个新模型。我们评估了从 15- 获得到 20-day-old 的 Sertoli 和 Leydig 房间新生的猪睾丸以纯净和功能。随后,净化了 Sertoli 并且充实 Leydig 房间受到 coincubation 获得一在里面 vitro prepubertal 猪的像睾丸的文化系统。我们为 anti-M 执行了连接酶的 immunosorbent 试金(ELISA ) | Iva Arato Giovanni Luca Francesca Mancuso Catia Bellucci Cinzia Lilli Mario Calvitti Barbara C Hansen Domenico Milardi Giuseppe Grande Riccardo Calafiore | 2018 | Asian Journal of Andrology2018，20，2： | 4 |
| 5 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 6 | Comparative transcriptomics of Central Asian Vitis vinifera accessions reveals distinct defense strategies against powdery mildew显示文摘Grape powdery mildew(PM),caused by the biotrophic ascomycete Erysiphe necator,is a devastating fungal disease that affects most Vitis vinifera cultivars.We have previously identified a panel of V.vinifera accessions from Central Asia with partial resistance to PM that possess a Ren1-like local haplotype.In this study,we show that in addition to the typical Ren1-associated late post-penetration resistance,these accessions display a range of different levels of disease development suggesting that alternative alleles or additional genes contribute to determining the outcome of the interaction with the pathogen.To identify potential Ren1-dependent transcriptional responses and functions associated with the different levels of resistance,we sequenced and analyzed the transcriptomes of these Central Asian accessions at two time points of PM infection.Transcriptomes were compared to identify constitutive differences and PM-inducible responses that may underlie their disease resistant phenotype.Responses to E.necator in all resistant accessions were characterized by an early up-regulation of 13 genes,most encoding putative defense functions,and a late down-regulation of 32 genes,enriched in transcriptional regulators and protein kinases.Potential Ren1-dependent responses included a hotspot of co-regulated genes on chromosome 18.We also identified 81 genes whose expression levels and dynamics correlated with the phenotypic differences between the most resistant accessions‘Karadzhandahal’,DVIT3351.27,and O34-16 and the other genotypes.This study provides a first exploration of the functions associated with varying levels of partial resistance to PM in V.vinifera accessions that can be exploited as sources of genetic resistance in grape breeding programs. | Katherine C H Amrine Barbara Blanco-Ulate Summaira Riaz Dániel Pap Laura Jones Rosa Figueroa-Balderas M Andrew Walker Dario Cantu | 2015 | Horticulture Research2015，2，1： | 3 |
| 7 | 扁桃体鳞癌中HPV感染与EGFR、VEGF表达的相关性研究显示文摘目的:研究扁桃体鳞癌(squamous cell carcinomas of the tonsil tonsillar,SCCs)中人乳头瘤病毒(human papillomavirus,HPV)感染与血管内皮生长因子(vascular endothelial growth factor,VEGF)和表皮生长因子受体(epidermal growth factor,EGFR)表达的关系,探讨HPV与EGFR和VEGF在扁桃体鳞癌发生中的交互效应。方法:采用多重实时荧光定量聚合酶链反应(multiplex real-time polymerase chain reaction,MT-PCR)检测85例扁桃体鳞癌HPV DNA及型别,通过HPV DNA分析进行HPV感染状况的测定;同时采用半定量免疫组化检测HPV(+)组和HPV(-)组中VEGF、EGFR蛋白表达情况。结果:扁桃体鳞癌中HPV感染率为49.4%(42/85),HPV-16型占所有感染者的比例为90.5%(38/42),明显高于其他型别;HPV(+)组EGFR蛋白表达明显低于HPV(-)组(P值均<0.01);HPV(+)组与HPV(-)组VEGF蛋白表达无明显差异;此外,VEGF表达与EGFR、患者的年龄、性别、TNM分期、组织学分级均无明显相关性。结论:HPV感染与扁桃体鳞癌的发生存在相关性,HPV相关的扁桃体鳞癌中,HPV基因可能通过改变EGFR表达致癌,为HPV致癌机理进一步研究提供一个新的视角,具有重要的理论意义。 | 费继敏 Timothy A Dobbins Deanna Jones C Soon Lee Christine Loo Jonathan Clark Barbara Rose | 2015 | 现代肿瘤医学2015，23，4： | 3 |
| 8 | 含高钙和维生素D的强化奶能有效提高健康的绝经后的中国女性体内的维生素D水平并减少骨吸收显示文摘目的钙摄入水平低以及体内维生素D不足是导致亚洲女性罹患骨质疏松的危险因素。此项研究的目的是评估高钙维生素D强化牛奶(HCM)对健康的中国绝经后妇女的体内维生素D状态和骨吸收标志物进行干预的影响。方法本试验对象为55岁以上,绝经至少5年的女性。共有124名女性进行了总体健康状况和骨密度检查筛选。63名女性被随机分为对照组和试验组,试验组在12w内每日饮用两次高钙/维生素D强化奶(含900 mg钙、96 mg镁、2.4 mg锌和6.4μg维生素D)。2组分别在试验开始、试验2 w、8 w和12 w时测量血矿物质、25(OH)维生素D3和Ⅰ型胶原C端肽(CTX)水平。结果对照组女性平均年龄为63±4.6岁,高钙强化奶(HCM)组女性平均年龄为62±3.8岁。所测得的每日钙摄入量的基线值为HCM组260~482 mg,对照组为252~692 mg。12 w后HCM组的平均身体质量指数(BMI)显著降低(P<0.05)。HCM组的血25(OH)维生素D3水平显著提高(33.13~39.49 nmol/L),而对照组则维持不变(29.27~28.21 nmol/L)。HCM组的血CTX水平在0 w和2 w之间下降了25%,并保持不变直至第12w。从第2w开始HCM组和对照组相比血CTX水平就出现了显著差异(P<0.001)。结论通过饮用含高钙和维生素D的强化奶(HCM),在2至4w内能显著提高中国绝经妇女体内维生素D水平,并减少骨吸收破坏。如果长期持续地饮用含高钙和维生素D的强化奶(HCM),能减少骨吸收和骨量丢失的风险,从而减少患骨质疏松和由此引起骨折的风险。 | Marlena C Kruger PengCheng Ha Joanne M Todd Barbara Kuhn-Sherlock Linda M Schollum Jiliang Ma George Qin Edith Lau | 2013 | 中国骨质疏松杂志2013，19，2： | 2 |
| 9 | OPCML对卵巢癌细胞抑制的体内外实验研究显示文摘目的探讨OPCML在体外、体内对卵巢癌细胞的影响。方法将OPCML用重组慢病毒转导入人卵巢癌细胞A2780、OCC1和正常小鼠卵巢上皮细胞CD1。通过细胞增殖试验、细胞聚合力试验、细胞周期的分析和体内成瘤试验等来研究OPCML在卵巢癌细胞中的功能。结果(1)OPCML能被重组慢病毒高效地转导入靶细胞,转导效率几乎达100%,同时达到稳定的表达,Westernblot能检测到OPCML(60kDa)和GFP(27kDa)基因蛋白在靶细胞中的表达;(2)在A2780细胞系,转导入OPCML后的细胞(A2780-OPCML)的增殖明显的比未转基因(A2780)或仅转空载体(A2780-pWPI)者慢(P<0.01),但在OCC1和CD1细胞系,OPCML对细胞的增殖无明显的影响(P>0.05);(3)用流式细胞仪法对细胞周期分析显示,OPCML对A2780的细胞周期有明显的滞留作用(P<0.05),但对OCC1、CD1细胞则无明显滞留作用;(4)细胞聚合力试验提示OPCML能明显增强细胞的粘附力;(5)表达OPCML的A2780细胞在裸鼠皮下仅有一个(1/4)有肿瘤生长,体积显著小于A2780及A2780-pWPI组(4/4)(P<0.001),免疫组织化学染色法能够检测到OPCML蛋白在肿瘤组织中的表达。结论慢病毒载体作为转基因的工具,具有高效转导率和稳定表达的特点;OPCML能够增加细胞的粘附能力,抑制A2780的增殖和体内成瘤,提示OPCML可能是一个新的抑癌基因。 | 姚德生 李力 Kenneth Garson Barbara C Vanderhyden | 2007 | 肿瘤防治研究2007，34，2： | 2 |
| 10 | Trabecular Bone Score: A Noninvasive Analytical Method Based Upon the DXA Image显示文摘 | Barbara C Silva William D Leslie Heinrich Resch Olivier Lamy Olga Lesnyak Neil Binkley Eugene V McCloskey John A Kanis John P Bilezikian | 2014 | J Bone Miner Res2014，，3： | 2 |
| 11 | Bloodstream infections caused by small-colony variants of coagulase-negative staphylococci following pacemaker implantation显示文摘 | yon Eiff C Vaudaux P Barbara C | 1999 | Clin Infect Dis1999，29，： | 1 |
| 12 | Corrosion kinetics of battery zinc alloys in electrolyte solutions 显示文摘 | BARBARA S MARIA C ALFRED N | 1998 | Journal of Power Sources1998，75，： | 1 |
| 13 | Microvascular dysfunction induced by reperfusion injure and protective effect of ischemia preconditioning 显示文摘 | Juan C Cutrin Maria-giulia Perrelli Barbara Cavalieri | 2002 | Free Radical Biology & Medicine2002，，9： | 1 |
| 14 | Antiproliferative effects of tocopherols (vitamin E) on murine glioma C6 cells:homologue-specific control of PKC/ERK and cyclin signaling显示文摘 | Michele B Andrea M Barbara C | 2006 | Free Radical Biology and Medicine2006，41，： | 1 |
| 15 | Evaluation of a transcription-mediated amplification-based HCV and HIV-1RNA duplex assay for screening individual blood donations: a comparison with a minipool testing system 显示文摘 | DANIEL C ALINE R BARBARA C | 2003 | Transfusion2003，43，2： | 1 |
| 16 | The impact of Er,Cr: YS- GG laser on the shear strength of the bond between dentin and ce- ramie is dependent on the adhesive material显示文摘 | Barbara C Gundula M J0rg W | 2012 | Lasers Med Sci2012，27，4： | 1 |
| 17 | Pulse Transit Time and Blood Pressure Changes Following Auditory-e- voked Subcortical Arousal and Waking of Infants 显示文摘 | Barbara C Galland Evan Tan Bar J Taylor | 2007 | Sleep2007，30，7： | 1 |
| 18 | Synthesis and antimycotic activity of new unsymmetrical substituted zinc phthalocyanines显示文摘 | Barbara C Gabrio R Donata D | 2003 | Tetrahedron2003，59，10： | 1 |
| 19 | Reexpansion pulmonary edema following a posttraumatic pneumothorax:a case report and review of the literature显示文摘 | Mark Malota Markus C Kowarik Barbara Bechtold | | Emerg Surg0，，： | 1 |
| 20 | Multidrug Resistance Proteins\sMRP3,MRP1,and MRP2 in Lung Cancer:Correlation of Protein Levels\swith Drug Response and Messenger RNA Levels显示文摘 | Leah C Barbara G Susan P | 2001 | Clinical Cance\sResearch2001，7，： | 1 |
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