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| 1 | Glial cell-derived neurotrophic factor mRNA expression in a rat model of spinal cord injury following bone marrow stromal cell transplantation显示文摘BACKGROUND: Several animal experiments utilizing bone marrow stromal cell (BMSC) transplantation for the treatment of spinal cord injury have proposed a hypothesis that BMSC transplantation effects are associated with increased glial cell-derived neurotrophic factor (GDNF) expression. OBJECTIVE: To confirm the effects of BMSC transplantation on GDNF mRNA expression in rats with spinal cord injury by reverse transcription-polymerase chain reaction (RT-PCR). DESIGN, TIME AND SETTING: The present molecular, cell biology experiment was performed at the Key Laboratory of Children's Congenital Malformation, Ministry of Health of China & Department of Developmental Biology, Basic Medical College, China Medical University between March 2006 and May 2007. MATERIALS: Sixty healthy Wistar rats aged 2-4-months and of either gender were included in this study. Spinal cord injury was induced in all rats by hemisection of T9 on the left side. RT-PCR kits were purchased from TaKaRa Company, China. Type 9600 RCR amplifier was provided by PerkinElmer Company, USA. METHODS: Three rats were selected for BMSC culture and subsequent transplantation (after three passages). Of the remaining 57 rats, nine were selected for sham-operation (sham-operated group), where only the T9 spinal cord was exposed without hemisection. A total of 48 rats were randomly and evenly divided into BMSC transplantation and model groups. In the BMSC transplantation group, following spinal cord injury induction, each rat was administered a BMSC suspension through two injection sites selected on the gray and white matter boundary caudally and cephalically, seperately and near to injury site in the spinal cord. The model group received an equal volume of PBS through the identical injection sites. MAIN OUTCOME MEASURES: At 24 and 72 hours, as well as at 7 days, following spinal cord injury, the spinal cord at the T 9 segment was removed. Eight rats were allocated to each time point in the BMSC transplantation and model groups, with three rats allocated to the sham-operated group. GDNF mRNA expression was semiquantitatively analyzed by RT-PCR. RESULTS: The sham-operated group exhibited extremely low GDNF mRNA expression. GDNF mRNA expression significantly increased at 24 hours after spinal cord injury, reached a peak level at 72 hours, and slowly decreased thereafter. However, it remained higher than normal levels at 7 days (P < 0.05). At all time points following spinal cord injury, GDNF mRNA expression was significantly greater in the BMSC transplantation group than in the model group (P < 0.05). CONCLUSION: Transplantation of BMSCs into the injured spinal cord up-regulated GDNF mRNA expression, thereby promoting repair of the injured spinal cord. | Lei Li Gang Lu Yanfeng Wang Hong Gao XinXu Lunhao Bai Lunhao Bai Huan Wang | 2008 | Neural Regeneration Research2008，3，10： | 13 |
| 2 | Blunted nitric oxide regulation in Tibetans under high-altitude hypoxia显示文摘Nitric oxide(NO) is an important molecule for vasomotor tone, and elevated NO signaling was previously hypothesized as a unique and adaptive physiological change in highland Tibetans. However, there has been lack of NO data from Tibetans living at low altitude and lowlander immigrants living at high altitude, which is crucial to test this hypothesis. Here, through cross-altitude(1990–5018 m) and cross-population(Tibetans and Han Chinese) analyses of serum NO metabolites(NOx) of 2086 individuals, we demonstrate that although Tibetans have a higher serum NOx level compared to lowlanders, Han Chinese immigrants living at high altitude show an even higher level than Tibetans. Consequently, our data contradict the previous proposal of increased NO signaling as the unique adaptive strategy in Tibetans.Instead, Tibetans have a relatively lower circulating NOx level at high altitude. This observation is further supported by data from the hypoxic experiments using human umbilical vein endothelial cells and gene knockout mice. No difference is detected between Tibetans and Han Chinese for endothelial nitric oxide synthase(e NOS), the key enzyme for circulating NO synthesis, suggesting that e NOS itself is unlikely to be the cause. We show that other NO synthesis-related genes(e.g. GCH1) carry Tibetan-enriched mutations significantly associated with the level of circulating NOx in Tibetans. Furthermore, gene network analysis revealed that the downregulation and upregulation of NOx is possibly achieved through distinct pathways.Collectively, our findings provide novel insights into the physiological and genetic mechanisms of the evolutionary adaptation of Tibetans to high-altitude hypoxia. | Yaoxi He Xuebin Qi Ouzhuluobu Shiming Liu Jun Li Hui Zhang Baimakangzhuo Caijuan Bai Wangshan Zheng Yongbo Guo Duojizhuoma Baimayangji Dejiquzong Bianba Gonggalanzi Yongyue Pan Qula Kangmin Cirenyangji Wei Guo Yangla Yi Peng Xiaoming Zhang Kun Xiang Zhaohui Yang Liangbang Wang Gengdeng Yanfeng Zhang Tianyi Wu Bing Su Chaoying Cui | 2018 | National Science Review2018，5，4： | 7 |
| 3 | Experimental investigation of ghost imaging of reflective objects with different surface roughness显示文摘We present an experimental demonstration of ghost imaging of reflective objects with different surface roughness.The influence of the surface roughness, the transverse size of the test detector, and the reflective angle on the signal-to-noise ratio(SNR) is analyzed by measuring the second-order correlation of the light field based on classical statistical optics. It is shown that the SNR decreases with an increment of the surface roughness and the detector's transverse size or a decrease of the reflective angle. Additionally, the comparative studies between the rough object and the smooth one under the same conditions are also discussed. | SUQIN NAN YANFENG BAI XIAOHUI SHI QIAN SHEN LIJIE QU HENGXING LI XIQUAN FU | 2017 | Photonics Research2017，5，4： | 5 |
| 4 | Positive influence of the scattering medium on reflective ghost imaging显示文摘The scattering medium is usually thought to have a negative effect on the imaging process.In this paper,it is shown that the imaging quality of reflective ghost imaging(GI)in the scattering medium can be improved effectively when the binary method is used.By the experimental and the numerical results,it is proved that the existence of the scattering medium is just the cause of this phenomenon,i.e.,the scattering medium has a positive effect on the imaging quality of reflective GI.During this process,the effect from the scattering medium behaves as the random noise which makes the imaging quality of binary ghost imaging have an obvious improvement. | QIN FU YANFENG BAI XIANWEI HUANG SUQIN NAN PEIYI XIE XIQUAN FU | 2019 | Photonics Research2019，7，12： | 3 |
| 5 | The C_(6)D_(6)detector system on the Back-n beam line of CSNS显示文摘Introduction The neutron capture cross sections are very important in the field of nuclear device design and basic physics research.Hydrogen-free liquid scintillator such as C_(6)D_(6)detectors are widely used in the neutron capture cross-sectional measurements for the low neutron sensitivity and fast time response.The Back-n white neutron source at China Spallation Neutron Source is the first spallation white neutron source in China,and it is suitable for neutron capture cross-sectional measurement.Materials and methods A C_(6)D_(6)detector system was built in the Back-n experimental station.The pulse height weighting technique was used to determine the system’s detection efficiency.The response to gamma rays of the C_(6)D_(6)detector was measured,and the energy resolution function was determined.Monte Carlo simulation with Geant4 code was carried out to get the weighting function of this C_(6)D_(6)detector system.Additionally,the systematic uncertainty of the weighting function was also determined.Conclusion According to the experimental and simulation results,this C_(6)D_(6)detector system can be used to measure neutron capture cross section. | Jie Ren Xichao Ruan Jie Bao Guangyuan Luan Wei Jiang Qi An Huaiyong Bai Ping Cao Qiping Chen Yonghao Chen Pinjing Cheng Zengqi Cui Ruirui Fan Changqing Feng Minhao Gu Fengqin Guo Changcai Han Zijie Han Guozhu He Yongcheng He Yuefeng He Hanxiong Huang Weiling Huang Xiru Huang Xiaolu Ji Xuyang Ji Haoyu Jiang Hantao Jing Ling Kang Mingtao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Rong Liu Shubin Liu Xingyan Liu Yinglin Ma Changjun Ning Binbin Qi Zhaohui Song Hong Sun Xiaoyang Sun Zhijia Sun Zhixin Tan Hongqing Tang Jingyu Tang Pengcheng Wang Qi Wang Taofeng Wang Yanfeng Wang Zhaohui Wang Zheng Wang Jie Wen Zhongwei Wen Qingbiao Wu Xiaoguang Wu Xuan Wu Likun Xie Yiwei Yang Han Yi Li Yu Tao Yu Yongji Yu Guohui Zhang Jing Zhang Linhao Zhang Liying Zhang Qingmin Zhang Qiwei Zhang Xianpeng Zhang Yuliang Zhang Zhiyong Zhang Yingtan Zhao Liang Zhou Zuying Zhou Danyang Zhu Kejun Zhu Peng Zhu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 6 | Ghost imaging for a reflected object with a rough sur- face显示文摘 | Wang Chunfang Zhang Dawei Bai Yanfeng | 2010 | Physical Reviews A2010，82，06： | 1 |
| 7 | 显示文摘 | Bai Yongxiao Li Yanfeng Yang Yong | 2006 | Journal of Bio-technology2006，125，4： | 1 |
| 8 | Superluminal optical solitons in a four-level tripod atomic system显示文摘 | HAN Dingan ZENG Yaguang BAI Yanfeng | 2006 | J Phys B2006，39，： | 1 |
| 9 | Study on synthesis of a hydrophilic bead carrier containing epoxy groups and its properties for glucoamylase immobilization显示文摘 | Bai Yongxiao Li Yanfeng Wang Mingtao | 2006 | Enzyme and Technology2006，39，： | 1 |
| 10 | Effects of spontaneously generated coherence on the conditions for exhibiting lasing without inversion in a V system显示文摘 | BAI Yanfeng HONG Guo HUI Sun | 2004 | Phys Rev A2004，69，： | 1 |
| 11 | Preparation of crosslinked macroporous PVA foam carrier for immobilization of microorgan- isms 显示文摘 | Bai Xue Ye Zhengfang Li Yanfeng | 2010 | Process Biochemistry2010，45，1： | 1 |
| 12 | Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity显示文摘 | Yong Yang Bai Yongxiao Li Yanfeng | 2008 | Process Biochemistry2008，43，： | 1 |
| 13 | A robust scheduling algorithm for space telescopes with unpredictable tasks显示文摘The original preset schedule of space telescopes can be disrupted when they receive ultra-high-priority unpredictable tasks.The main objective of this study is to observe such unpredictable tasks,the instability of which leads to failures in delivering the promises made.This paper proposes a method for retaining the robustness of the original plan.We use space-based multi-band variable objects monitor(SVOM)mission as an example to introduce the proposed method.First,the reasons for instability are discussed in detail.Two proactive strategies are proposed to promote the robustness of the schedule.The proactive strategies use the available windows of a given list of jobs.However,the realistic problem of SVOM is analysed using a rescheduling algorithm based on NSGA-II experiments with SVOM scenarios showing that the two proposed strategies are effective in reducing the instability caused by unpredictable interruption. | LI DaLin GU YanFeng JAUBERT Jean LIU YuRong BAI Meng FENG Zhun | 2021 | Science China(Technological Sciences)2021，64，3： | 1 |
| 14 | Preparation and application of polymer-grafted magnetic nanoparticles for lipase immobilization 显示文摘 | Yang Yong Bai Yongxiao Li Yanfeng | 2008 | Journal of Magnetism and Magnetic Materials2008，320，19： | 1 |
| 15 | Study on immobilization of lipase onto magnetic microspheres with epoxy groups显示文摘 | Lin Lei Yongxiao Bai Yanfeng Li | 2009 | Journal of Magnetism and Magnetic Materials2009，，321： | 1 |
| 16 | Characterization of candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity显示文摘 | YONG YANG BAI YONGXIAO LI YANFENG | 2008 | Process Bi- ochemistry2008，43，11： | 1 |
| 17 | Preparation and characterization of crosslinked porous cellulose beads显示文摘 | BAI Yongxiao LI Yanfeng | 2006 | Carbohydrate Polymers2006，64，3： | 1 |
| 18 | Lynch syndrome pre-screening and comprehensive characterization in a multi-center large cohort of Chinese patients with colorectal cancer显示文摘Objective:Lynch syndrome(LS)pre-screening methods remain under-investigated in colorectal cancers(CRCs)in Asia.Here,we aimed to systematically investigate LS pre-screening and comprehensively characterize LS CRCs.Methods:Microsatellite instability(MSI)and germline variants of DNA mismatch repair(MMR)genes were examined in 406 deficient MMR(dMMR)and 250 proficient MMR CRCs.The genetic differences between LS and sporadic CRCs were studied with whole exome sequencing analysis.Results:The incidence of dMMR in Chinese patients with CRCs was 13.8%.Consistency analysis between MMR immunohistochemistry(IHC)and MSI testing showed the kappa value was 0.758.With next-generation sequencing(NGS),germline variants were detected in 154 CRCs.Finally,88 patients with CRC were identified as having LS by Sanger sequencing.Among them,we discovered 21 previously unreported pathogenic germline variants of MMR genes.Chinese patients with LS,compared with sporadic CRCs,tended to be early-onset,right-sided,early-stage and mucinous.Overall,the performance of MMR IHC and MSI testing for LS pre-screening was comparable:the area under the ROC curve for dMMR,MSI-H,and MSI-H/L was 0.725,0.750,and 0.745,respectively.dMMR_MSI-H LS and sporadic CRCs showed substantial differences in somatic genetic characteristics,including different variant frequencies of APC,CREBBP,and KRAS,as well as different enriched pathways of VEGF,Notch,TGFβR,mTOR,ErbB,and Rac protein signal transduction.Conclusions:MMR IHC and MSI testing were effective methods for LS pre-screening.The revealed clinical and somatic genetic characteristics in LS CRCs may have the potential to improve the performance of LS pre-screening in combination with dMMR/MSI. | Yan Li Lihong Fan Jianming Zheng Xiu Nie Yu Sun Qin Feng Shenyi Lian Wenqi Bai Weijing Cai Yanan Yang Bo Su Yanfeng Xi Dongmei Lin | 2022 | Cancer Biology & Medicine2022，19，8： | 0 |
| 19 | Damage Characteristics and Control Measures of Maize Head Smut显示文摘Maize head smut is a worldwide disease,widely occurred in most maize fields. The disease had occurred in large area in the late 1970 s,and became a limiting factor restricting the improvement of maize yield and total yield in China. After efforts of several generations of agricultural scientific workers,the symptoms,pathogen,infection pathway,infection conditions,infection regularity and resistance genetic law of maize head smut have been studied. This paper introduces the incidence symptoms,pathogen sources,infection pathway,infection conditions,and physiological races of maize head smut,and put forwards the corresponding prevention measures,such as crop rotation,selection of resistant varieties,seed dressing,soil treatment with fungicides,promoting seedling emergence and strengthening field management. | Bai Yanfeng Jiang Long Ren Haixiang Wang Yanping Wang Xiaomei Zong Chunmei Sun Xiaohuan Qi Yuxin | 2014 | Plant Diseases and Pests2014，5，5： | 0 |
| 20 | Two decades of tax-sharing system reform in China:a comparative study显示文摘Two decades have passed since the Tax-sharing System Reform began in China.We discuss deep-seated problems in the financial management system.We contend that from the point of fiscal relations among different levels of government,the marked decline of the central government’s fiscal revenue as a share of the national total indicates that the macroeconomic regulation and control function of the central government has weakened;from the point of the form of fiscal revenue,the constant downward trend of the proportion of the tax revenue indicates that the old problem of excessive types of fiscal revenue forms have reappeared;from the perspective of tax structure,although the proportion of direct taxes has grown,turnover tax continues to account for the majority of tax revenue and the unbalanced dual-subject tax system has changed little,which indicates China’s tax structure has brought about stagnation.We believe that China’s tax reform should be incorporated into the medium-term fiscal planning in order to solve deep-seated problems in operation of the financial management system. | Bai Yanfeng Wang Kai | 2015 | China Finance and Economic Review2015，4，1： | 0 |
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