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| 1 | Evidence chain-based causality identification in herb-induced liver injury: exemplification of a well-known liver-restorative herb Polygonum multiflorum显示文摘草药的药最近在美国作为导致药的肝损害(DILI ) 的第二个很普通的原因被认出了。然而,识别一些植物的 DILI 诱发性的可靠方法例如 Heshouwu (蓼 multiflorum 的弄干的根) ,留下缺乏。在这研究,有肝机能障碍和 Heshouwu DILI 的 147 个报导文学的案例的 12 307 个住院病人的一个总数被屏蔽。一个一般算法仅仅显示了那 22.5%(9/40 ) 并且(45/147 ) 分别地, 30.6% 所有住院和文学案例报告表明 Heshouwu 的 DILI 诱发性的高概率。由对比,(19/20 )95% 生药学,植物化学,和 metabolomic 有希望地调查的所有情况测试展出高度可能的诱发性,包括不正确地以前被归因的一个病人和从由 pharmacognostic 证据的 Heshouwu 诱发性被排除的一个案例。污染也是的毒素(重金属,杀虫剂,和 mycotoxins ) 从 Heshouwu DILI 诱发性排除了。为 Heshouwu DILI 诊断地址安全的这些屏蔽方法的客观性担心考虑包含芪的草药的药和饮食的补充。 | Jiabo Wang Zhijie Ma Ming Niu Yun Zhu Qingsheng Liang Yanling Zhao Jingyuan Song Zhaofang Bai Yaming Zhang Ping Zhang Na Li Yakun Meng Qi Li Lushan Qin Guangju Teng Junling Cao Baosen Li Shilin Chen Yonggang Li Zhengsheng Zou Honghao Zhou Xiaohe Xiao | 2015 | Frontiers of Medicine2015，9，4： | 47 |
| 2 | Screening for main components associated with the idiosyncratic hepatotoxicity of a tonic herb, Polygonum multiflorum显示文摘典型药用的植物的主要成分,蓼 multiflorum (用汉语的 Heshouwu ) ,那导致特质的肝损害仍然保持不清楚。我们的以前的工作证明了有 lipopolysaccharide (LPS ) 的无毒的剂量和 Heshouwu 的治疗学的剂量的 cotreatment 能在老鼠导致肝损害,而独唱治疗不能导致看得见的损害。在现在的工作,用成分大美人和敲门在里面策略,我们发现 Heshouwu 的乙醇醋酸盐(EA ) 摘录在对待 LPS 的老鼠显示了可比较的特质的 hepatotoxicity 到整个摘录。结果显示血浆丙氨酸 aminotransferase, aspartate aminotransferase,和肝 histologic 的重要举起变化,而另外的分开的部分没能导致肝损害。有另外的分开的部分的 EA 摘录的混合在对待 LPS 的老鼠导致了可比较的特质的 hepatotoxicity 到整个摘录。化学分析进一步揭示了那 2,3,5,4-tetrahydroxy trans-stilbene-2-O -- 配糖物(trans-SG ) 和它的 cis 异构体是在 EA 的二主要混合物摘录。而且,孤立的 cis- ,并且不它的 trans 异构体,显示的可比较的特质的 hepatotoxicity 到在对待 LPS 的老鼠的 EA 摘录。cis-SG 的更高的内容从与收集的将军相比与 Heshouwu 联系的实际的肝沉醉病人在 Heshouwu 白酒或准备被检测样品。另外,血浆 metabolomics 分析证明 cis-SG-disturbing 充实显著地不同于的小径在对待 LPS 的老鼠的 trans-SG。所有这些结果建议 cis-SG 仔细与 Heshouwu 的特质的 hepatotoxicity 被联系。认为 trans-SG 的 cis-trans 异构化被紫外光或日光调停,我们的调查结果为在药发现控制 photoisomerization 并且为 Heshouwu 和芪相关的药的临床的使用用作参考。 | Chunyu Li Ming Niu Zhaofang Bai Congen Zhang Yanling Zhao Ruiyu Li Can Tu Huifang Li Jing Jing Yakun Meng Zhijie Ma Wuwen Feng Jinfa Tang Yun Zhu Jinjie Li Xiaoya Shang Zhengsheng Zou Xiaohe Xiao Jiabo Wang | 2017 | Frontiers of Medicine2017，11，2： | 41 |
| 3 | Human Gut Microbiota and Gastrointestinal Cancer显示文摘Human gut microbiota play an essential role in both healthy and diseased states of humans.In the past decade,the interactions between microorganisms and tumors have attracted much attention in the efforts to understand various features of the complex microbial communities,as well as the possible mechanisms through which the microbiota are involved in cancer prevention,carcinogenesis,and anti-cancer therapy.A large number of studies have indicated that microbial dysbiosis contributes to cancer susceptibility via multiple pathways.Further studies have suggested that the microbiota and their associated metabolites are not only closely related to carcinogenesis by inducing in?ammation and immune dysregulation,which lead to genetic instability,but also interfere with the pharmacodynamics of anticancer agents.In this article,we mainly reviewed the in?uence of gut microbiota on cancers in the gastrointestinal(GI)tract(including esophageal,gastric,colorectal,liver,and pancreatic cancers)and the regulation of microbiota by diet,prebiotics,probiotics,synbiotics,antibiotics,or the Traditional Chinese Medicine.We also proposed some new strategies in the prevention and treatment of GI cancers that could be explored in the future.We hope that this review could provide a comprehensive overview of the studies on the interactions between the gut microbiota and GI cancers,which are likely to yield translational opportunities to reduce cancer morbidity and mortality by improving prevention,diagnosis,and treatment. | Changting Meng Chunmei Bai Thomas D. Brown Leroy E. Hood Qiang Tian | 2018 | Genomics, Proteomics & Bioinformatics2018，16，1： | 43 |
| 4 | Immunological synergistic mechanisms of trans-/cis-stilbene glycosides in Heshouwu-related idiosyncratic liver injury显示文摘Heshouwu is a traditional non-toxic Chinese medicine commonly used in the clinical setting;however,clinical cases of Heshouwu-induced hepatotoxicity have been frequently reported^([1]).Pharmacoepidemiological studies have found that Heshouwu-induced liver injury occurs only in a small fraction of individuals taking the drug;these patients presented with the typ- | Lanzhi He Ping Yin Yakun Meng Jinfa Tang Tingting He Ming Niu Yuming Guo Yun Zhu Jing Jing Chunyu Li Zhijie Ma Jiabo Wang Zhaofang Bai Xiaohe Xiao | 2017 | Science Bulletin2017，62，11： | 29 |
| 5 | Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ显示文摘蓼 multiflorum Thunb (下午) 的根在中国被使用了治疗许多疾病,例如便秘,头发和 hyperlipemia 的早 graying。最近的证据证明那位首相在人引起特质的导致药的肝损害(IDILI ) 。在这研究,我们基于 LPS 的非肝毒素的剂量在 IDILI 的一个老鼠模型调查了导致下午的肝损害的分子的基础。SD 老鼠口头上地被管理下午的 3 潜在地肝毒素的混合物:cis 芪配糖物(cis-SG, 50 mg/kg ) , trans-SG (50 mg/kg ) 或 emodin (5 mg/kg ) ,由 LPS 的注射列在后面(2.8 mg/kg, iv ) 。浆液和肝组织学被评估在 LPS 以后的 7 h 注射。在测试的 3 混合物之中, cis-SG,然而并非 emodin 或 trans-SG,当与 LPS 结合了时,在老鼠导致了严重的肝损害。在血浆的著名计算机生产厂商和在血浆和肝纸巾的煽动性的 cytokines 的层次显著地被提高。肝纸巾显示出增加的损害, hepatocyte apoptosis,和巨噬细胞渗入,并且减少房间增长。Microarray 分析揭示了在 peroxisome 之间的否定关联激活 proliferator 的受体 --(PPAR-) 并且 LPS/cis-SG-induced 肝损害。Immunohistochemical 染色和 RT-PCR 结果进一步证实 cis-SG 显著地在 LPS/cis-SG-treated 老鼠的肝纸巾禁止了 PPAR- 小径的激活。有 PPAR- 收缩筋 pioglitazone 的预告的处理(500 g/kg, ig ) 颠倒的 LPS/cis-SG-induced 肝损害,它与禁止原子因素 kappa B (NF-B ) 被联系小径。这些数据证明 cis 芪配糖物导致免疫学的通过在 IDILI 的一个老鼠模型压制 PPAR- 的特质的 hepatotoxicity。 | Ya-kun MENG Chun-yu LI Rui-yu LI Lan-zhi HE He-rong CUI Ping YIN Cong-en ZHANG Peng-yan LI Xiu-xiu SANG Ya WANG Ming NIU Ya-ming ZHANG Yu-ming GUO Rong SUN Jia-bo WANG Zhao-fang BAI Xiao-he XIAO | 2017 | Acta Pharmacologica Sinica2017，38，10： | 30 |
| 6 | In-Season Root-Zone Nitrogen Management Strategies for Improving Nitrogen Use Efficiency in High-Yielding Maize Production in China显示文摘Many recently developed N management strategies have been extremely successful in improving N use efficiency.How-ever,attempts to further increase grain yields have had limited success.Field experiments were conducted in 2007 and 2008 at four sites to evaluate the effect of an in-season root-zone N management strategy on maize (Zea mays L.).According to the in-season root-zone N management,the optimal N rate (ONR) was determined by subtracting measured soil mineral N (NH + 4--N and NO 3--N) in the root zone from N target values.Other treatments included a control without N fertilization,70% of ONR,130% of ONR,and recommended N rate (RNR) by agronomists in China that have been shown to approach maize yield potentials.Although apparent N recovery for the ONR treatment was significantly higher than that under RNR in 2007,grain yield declined from 13.3 to 11.0 Mg ha 1 because of an underestimation of N uptake.In 2008,N target values were adjusted to match crop uptake,and N fertilization rates were reduced from 450 kg N ha-1 for RNR to 225 to 265 kg N ha-1 for ONR.High maize yields were maintained at 12.6 to 13.5 Mg ha 1,which were twice the yield from typical farmers' practice.As a result,apparent N recovery increased from 29% to 66%,and estimated N losses decreased significantly for the ONR treatment compared to the RNR treatment.In conclusion,the in-season root-zone N management approach was able to achieve high yields,high NUE and low N losses. | MENG Qing-Feng CHEN Xin-Ping ZHANG Fu-Suo CAO Ming-Hui CUI Zhen-Ling BAI Jin-Shun YUE Shan-Chao CHEN Su-Yi T.MULLER | 2012 | Pedosphere2012，22，3： | 22 |
| 7 | Differential expression of serum miR-126,miR-141 and miR-21 as novel biomarkers for early detection of liver metastasis in colorectal cancer显示文摘Objective:MicroRNAs(miRNAs) have potential as diagnostic biomarkers in cancer.Evaluation of the association between miRNA expression patterns and early detection of liver metastasis in colorectal cancer(CRC) has not been reported.Methods:We investigated the expression of metastasis-associated miRs-31,335,206,141,126,200b,200c,21,Let7a,Let7b and Let7c in localized,liver-metastatic and other organ-metastatic CRC(OM-CRC).Expressions of target miRNAs in serum were evaluated in 116 consecutive localized CRC(L-CRC),72 synchronous liver-metastatic CRC(SLM-CRC) and 36 other OM-CRC by quantitative real-time PCR.Results:Seven of 11 tested miRNAs could be detected from serum.Four miRNAs,miR-126,Let-7a,miR-141 and miR-21 were identified as metastasis-associated miRNAs.Compared with L-CRC,significant upregulated expression was observed for miR-141 and miR-21 in SLM-CRC and OM-CRC,down-regulated expression was observed for miR-126 in SLM-CRC and OM-CRC,and up-regulated expression of Let-7a in OM-CRC.The receiver operating characteristic(ROC) curve showed serum miR-126 had a cut-off [log 10 relative quantity(log 10 RQ)=–0.2005] with 77.78% sensitivity and 68.97% specificity with an area under curve(AUC) of 0.7564,miR-141 had a cut-off(log 10 RQ=–0.2285) with 86.11% sensitivity and 76.11% specificity with an AUC of 0.8279,and miR-21 had a cut-off(log 10 RQ=–0.1310) with 73.61% sensitivity and 66.38% specificity with an AUC of 0.7479.Conclusions:We identified liver metastasis-associated miRNAs,suggesting serum miR-126,miR-141 and miR-21 may be novel biomarkers for clinical diagnosis of early stage liver-metastatic CRC. | Jie Yin Zhigang Bai Jianning Song Yun Yang Jin Wang Wei Han Jun Zhang Hua Meng Xuemei Ma Yao Yang Tingting Wang Weirong Li Zhongtao Zhang | 2014 | Chinese Journal of Cancer Research2014，26，1： | 22 |
| 8 | Experimental study on hydrodynamic effect of orientation micro-pored surfaces显示文摘The orientation of the dimple increases the flow distance in the dimple and produces fluid cumulative effect in the dimple length direction, which leads to obvious hydrodynamic effect as a result. In order to investigate the hydrodynamic effect of orientation dimples, a series of experiments was carried out on a ring-on-ring test. Multi-pored faces were tested with different dimple inclination angles and slender ratios. Film thickness and frictional torque were measured under different conditions of load and rotation speed. Experimental results showed that the orientation dimple could produce obvious dynamic effect by change of the flow direction and the increasing dimple orientation leads to increase of the load capability. The hydrodynamic effect strongly depends on dimple orientation parameters such as inclination angle and slender ratio. A larger load capability can be available by increasing dimple orientation and rotation speed. Experimental results agreed well with the theory that orientation micro-pores can significantly improve hydrodynamic performance of surfaces. | BAI ShaoXian1,2, PENG XuDong1,2, LI JiYun1,2 & MENG XiangKai1,2 1College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou 310032, China 2Key Laboratory of Special Purpose Equipment and Advanced Processing Technology, Ministry of Education, Zhejiang University of Technology, Hangzhou 310032, China | 2011 | Science China(Technological Sciences)2011，54，3： | 20 |
| 9 | Identification of coastal wetlands of international importance for waterbirds:a review of China Coastal Waterbird Surveys 2005–2013显示文摘Background:China's coastal wetlands belong to some of the most threatened ecosystems worldwide.The loss and degradation of these wetlands seriously threaten waterbirds that depend on wetlands.Methods:The China Coastal Waterbird Census was organized by volunteer birdwatchers in China's coastal region.Waterbirds were surveyed synchronously once every month at 14 sites,as well as irregularly at a further 18 sites,between September 2005 and December 2013.Results:A total of 75 species of waterbirds met the 1 % population level Ramsar listing criterion at least once at one site.The number of birds of the following species accounted for over 20 % of the total flyway populations at a single site:Mute Swan(Cygnus olor),Siberia Crane(Grus leucogeranus),Far Eastern Oystercatcher(Haematopus osculans),Bar-tailed Godwit(Limosa lapponica),Spotted Greenshank(Tringa guttifer),Great Knot(Calidris tenuirostris),Spoon-billed Sandpiper(Calidris pygmeus),Saunders' s Gull(Larus saundersi),Relict Gull(Larus relictus),Great Cormorant(Phalacrocorax carbo),Eurasian Spoonbill(Platalea leucorodia),Black-faced Spoonbill(Platalea minor) and Dalmatian Pelican(Pelecanus crispus).A total of 26 sites supported at least one species of which their number met the1 % criterion.Forty-two species met the 1 % criterion in the Yellow River Delta,Shandong;29 at the Cangzhou coast,Hebei and 26 species at the Lianyungang coast,Jiangsu.Conclusions:The results highlight the international importance of China's coastal wetlands for waterbirds.This study also demonstrates that participation of local birdwatchers in waterbird surveys results in data that are invaluable not only for understanding the current status of waterbirds in China's coastal regions but also for waterbird conservation and management. | Qingquan Bai Jianzhong Chen Zhihong Chen Guotai Dong Jiangtian Dong Wenxiao Dong Vivian Wing Kan Fu Yongxiang Han Gang Lu Jing Li Yang Liu Zhi Lin Derong Meng Jonathan Martinez Guanghui Ni Kai Shan Renjie Sun Suixing Tian Fengqin Wang Zhiwei Xu Yat-tung Yu Jin Yang Zhidong Yang Lin Zhang Ming Zhang Xiangwu Zeng | 2015 | Chinese Birds2015，，3： | 20 |
| 10 | Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS-LRR Protein Class Gene in Apple (Golden Delicious)显示文摘MicroRNA (miRNA ) 在植物作为基因表示的一个重要管理者出现了。146 miRNAs 从苹果被识别(Malus domestica cv。金色美味) 由定序的生物信息的分析和 RNA 图书馆。从这些, 135 被保存, 11 是新奇 miRNAs。目标分析预言了新奇 miRNAs 之一, Md-miRLn11 (Malus domestica microRNA Ln11 ) ,指向了一个苹果核苷酸绑定地点(NBS ) 充满白氨酸的重复(LRR ) 班蛋白质编码基因(Md-NBS ) 。5 赛跑试金证实了 Md-miRLn11 的能力在 1112-nt 位置劈开 Md-NBS。Md-miRLn11 和 Md-NBS 的表示的分析在在 40 个苹果变化的最佳侵略时期期间证明在抵抗变化的 Md-NBS 基因的表示比在易受影响的变化高,与为 Md-miRLn11 的一个反的模式。从抵抗苹果变化 JiGuan 的幼苗被用来执行 Agrobacterium 渗入试金,然后与苹果叶点疾病接种。结果在 JiGuan 苹果显示出疾病抵抗的清楚的衰落。相反,与 Md-NBS 基因渗入的易受影响的变化富士在疾病抵抗显示出重要增加。基于上述结果,我们建议 Md-miRLn11 特别地在病原体感染的条件下面调整 Md-NBS 基因表示,并且指向 P 环地点的 Md-miRLn11 可以在木质的植物调整许多 NBSLRR 蛋白质类基因。 | Chao Ma You Lu Songlin Bai Wennan Zhang Xuwei Duan Dong Meng Zhigang Wang Aide Wang Zongshan Zhou Tianzhong Li | 2014 | Molecular Plant2014，7，1： | 19 |
| 11 | GYY4137 protects against myocardial ischemia and reperfusion injury by attenuating oxidative stress and apoptosis in rats显示文摘Hydrogen sulfide(H_2S) is a gasotransmitter that regulates cardiovascular functions.The present study aimed to determine the protective effect of slow-releasing H_2S donor GYY4137 on myocardial ischemia and reperfusion(I/R) injury and to investigate the possible signaling mechanisms involved.Male Sprague-Dawley rats were treated with GYY4137 at 12.5 mg/(kg·day),25 mg/(kg·day) or 50 mg/(kg·day) intraperitoneally for7 days.Then,rats were subjected to 30 minutes of left anterior descending coronary artery occlusion followed by reperfusion for 24 hours.We found that GYY4137 increased the cardiac ejection fraction and fractional shortening,reduced the ischemia area,alleviated histological injury and decreased plasma creatine kinase after myocardial I/R.Both H_2S concentration in plasma and cystathionine-γ-lyase(CSE) activity in the myocardium were enhanced in the GYY4137 treated groups.GYY4137 also decreased malondialdehyde and myeloperoxidase levels in serum,attenuated superoxide anion level and suppressed phosphorylation of mitogen activated protein kinases in the myocardium after I/R.Meanwhile,GYY4137 increased the expression of Bcl-2 but decreased the expression of Bax,caspase-3 activity and apoptosis in the myocardium.The data suggest that GYY4137 protects against myocardial ischemia and reperfusion injury by attenuating oxidative stress and apoptosis. | Guoliang Meng Jing Wang Yujiao Xiao Wenli Bai Liping Xie Liyang Shan Philip K Moore Yong Ji | 2015 | The Journal of Biomedical Research2015，29，3： | 18 |
| 12 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 13 | Functional organization of complex brain networks modulated by acupuncture at different acupoints belonging to the same anatomic segment显示文摘 | CHEN Shang-jie MENG Lan YAN Hao BAI Li-jun WANG Fang HUANG Yong LI Jian-ping PENG Xu-ming SHI Xue-min | 2012 | Chinese Medical Journal2012，，15： | 12 |
| 14 | Osteopontin knockdown suppresses the growth and angiogenesis of colon cancer cells显示文摘AIM:To investigate the effects of osteopontin(OPN)gene expression knockdown on colon cancer Lovo cells in vitro.METHODS:Four candidate small interfering RNA(siRNA)constructs targeting the OPN gene and a scrambled control sequence(NC-siRNA)were synthesized and inserted into a pGPU6/GFP/Neo expression vector.After confirmation by restriction enzyme digestion and DNA sequencing,the recombinant plasmids were subsequently transfected into a human colon cancer cell line(Lovo)using a liposome transfection method.Stably transfected cells were maintained with G418 selection and referred to as Lovo-OPN-1,-2,-3,-4,and Lovo-NC cells.Knockdown efficiency of each of the four siRNA constructs was determined by realtime reverse transcription polymerase chain reaction assays and western blotting,and the construct with the most effective silencing was used for subsequent experiments.Cell proliferation,adhesion,and Matrigel invasion assays were performed to analyze the effects of OPN knockdown in stably transfected Lovo cells.The levels of four angiogenic factors,namely vascular endothelial growth factor(VEGF),matrix metalloproteinase(MMP)-2,MMP-9 and urokinase plasminogen activator were detected by enzyme-linked immunosorbent assays(ELISA).RESULTS:Recombinant vectors containing OPNspecific and scrambled siRNA sequences were successfully constructed and stably transfected into Lovo cells.Compared with the control Lovo and Lovo-NC cells,the levels of OPN mRNA and protein expression in LovoOPN-1,-2,-3,and-4 were significantly reduced(all P<0.05),with the most efficient reduction observed in Lovo-OPN-4 cells(P<0.05).Relative to untransfected Lovo cells,OPN mRNA expression levels in Lovo-NC and Lovo-OPN-4 cells were 1.008±0.067 and 0.160±0.023,respectively.The relative OPN protein expression levels in Lovo,Lovo-NC,and Lovo-OPN-4 cells were 3.024±0.211,2.974±0.630,and 0.121±0.008,respectively.Moreover,transfection with the scrambled sequence had no effect on the expression of OPN.After24,48,72,and 96 h of cultivation,absorption values at 450 nm to assess proliferation of Lovo-OPN-4 cells were 0.210±0.017,0.247±0.024,0.314±0.037,and 0.359±0.043,respectively,which were significantly lower than those of Lovo(0.244±0.031,0.313±0.024,0.513±0.048 and 0.783±0.051)and LovoNC cells(0.241±0.029,0.309±0.022,0.563±0.023,and 0.735±0.067)(all P<0.05).The absorption values at 595 nm,which were measured in a cell adhesion assay,showed that adhesion of Lovo-OPN-4 cells(0.215±0.036)was significantly decreased compared to Lovo(0.490±0.037)and Lovo-NC cells(0.462±0.043)(P<0.05).The number of invasive Lovo-OPN-4 cells(16.1±1.9)was also significantly decreased compared to Lovo(49.9±5.4)and Lovo-NC cells(48.8±4.5)(P<0.05).ELISA assays showed significant reductions in Lovo-OPN-4 cells compared to Lovo and Lovo-NC cells with regard to the expression of VEGF(1687.85±167.84 ng/L vs 2348.54±143.80 ng/L and 2284.39±138.62 ng/L,respectively),MMP-2(2966.07±177.36μg/L vs 4084.74±349.54μg/L and 4011.41±424.48μg/L,respectively),MMP-9(3782.89±300.64μg/L vs5062.90±303.02μg/L and 4986.38±300.75μg/L,respectively)and uPA(1152.69±120.79μg/L vs1380.90±147.25μg/L and 1449.80±189.92μg/L,respectively)(all P<0.05).CONCLUSION:Knockdown of OPN gene expression suppresses colon cancer cell growth,adherence,invasion,and expression of angiogenic factors. | Xin-Lin Wu Kai-Jin Lin Ai-Ping Bai Wan-Xiang Wang Xing-Kai Meng Xiu-Lan Su Ming-Xing Hou Pei-De Dong Jun-Jing Zhang Zhao-Yang Wang Lin Shi | 2014 | World Journal of Gastroenterology2014，20，30： | 12 |
| 15 | Gold nanorod-photosensitizer conjugate with extracellular pH-driven tumor targeting ability for photothermal/photodynamic therapy显示文摘氯 e6-pHLIP <候补选手class=“ a-plus-plus ”> ss -AuNRs,金使nanorod具有感光性结合包含 pH (低)有给予细胞外的 pH 的一张二硫化物契约的插入肽( pHLIP )( pH <候补选手class=“ a-plus-plus ”> e )-driven 肿瘤指向能力,成功地为光力学的 bimodal 和 photothermal 治疗被开发了。在这 bimodal 治疗,氯 e6 (Ce6 ) ,第二代的 photosensitizer (PS ) ,被用于光力学的治疗(太平洋夏季时间) 。金 nanorods (AuNRs ) 作为 Ce6 的 nanocarrier 和 quencher 为 photothermal 治疗(PTT ) 并且也被用作一个过高热代理人。pHLIPss 作为一根驾驶 pHe 的指向探针被设计在低 pH 在癌症房间提高 Ce6 和 AuNRs 的累积。在 Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs, Ce6 接近并且由 AuNRs 熄灭了,引起小太平洋夏季时间效果。当暴露了到正常生理的 pH 时 7.4, Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs 泛泛地与房间膜联系。然而,曾经暴露了到酸的 pH 6.2, pHLIP 活跃地插入到房间膜,和 conjugates 是进房间的 translocated。当这发生时, Ce6 由于二硫化物与 AuNRs 分开细胞内部的谷胱甘肽(GSH ) 引起的契约劈开,和汗衫氧在光之上为太平洋夏季时间被生产照耀。作为单个 PTT 代理人,另外, AuNRs 能由提高的浸透和保留(EPR ) 效果在肿瘤提高 PS 的累积。因此由我们的数据显示了当暴露了到酸的 pH 时, Ce6-pHLIP < 潜水艇 class= “ a-plus-plus ” > ss -AuNRs 能为癌症治疗完成 synergistic PTT/PDT bimodality。 | Nannan Wang Zilong Zhao Yifan Lv Huanhuan Fan Huarong Bai Hongmin Meng Yuqian Long Ting Fu Xiaobing Zhang Weihong Tan | 2014 | Nano Research2014，7，9： | 11 |
| 16 | Plasma microRNAs as potential new biomarkers for early detection of early gastric cancer显示文摘BACKGROUND Early gastric cancer(EGC), compared with advanced gastric cancer(AGC), has a higher 5-year survival rate. However, due to the lack of typical symptoms and the difficulty in diagnosing EGC, no effective biomarkers exist for the detection of EGC, and gastroscopy is the only detection method.AIM To provide new biomarkers with high specificity and sensitivity through analyzed the differentially expressed microRNAs(miRNAs) in EGC and AGC and compared them with those in benign gastritis(BG).METHODSWe examined the differentially expressed miRNAs in the plasma of 30 patients with EGC, AGC, and BG by miRNA chip analysis. Then, we analyzed and selected the significantly different miRNAs using bioinformatics. Reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR)confirmed the relative transcription level of these miRNAs in another 122 patients, including patients with EGC, AGC, Helicobacter pylori(H. pylori)-negative gastritis(Control-1), and H. pylori-positive atrophic gastritis(Control-2).To establish a diagnostic model for the detection of plasma miRNA in EGC, we chose miRNAs that can be used to determine EGC and AGC from Control-1 and Control-2 and miRNAs in EGC from all other groups.RESULTS Among the expression profiles of the miRNA chips in the three groups in the discovery set, of 117 aberrantly expressed miRNAs, 30 confirmed target prediction, whereas 14 were included as potential miRNAs. The RT-qPCR results showed that 14 potential miRNAs expression profiles in the two groups exhibited no differences in terms of H. pylori-negative gastritis(Control-1) and H. pyloripositive atrophic gastritis(Control-2). Hence, these two groups were incorporated into the Control group. A combination of four types of miRNAs,miR-7641, miR-425-5 p, miR-1180-3 p and miR-122-5 p, were used to effectively distinguish the Cancer group(EGC + AGC) from the Control group [area under the curve(AUC) = 0.799, 95% confidence interval(CI): 0.691-0.908, P < 0.001].Additionally, miR-425-5 p, miR-24-3 p, miR-1180-3 p and miR-122-5 p were utilized to distinguish EGC from the Control group(AUC = 0.829, 95%CI: 0.657-1.000, P =0.001). Moreover, the miR-24-3 p expression level in EGC was lower than that in the AGC(AUC = 0.782, 95%CI: 0.571-0.993, P = 0.029), and the miR-4632-5 p expression level in EGC was significantly higher than that in AGC(AUC = 0.791,95%CI: 0.574-1.000, P = 0.024).CONCLUSION The differentially expressed circulatory plasma miR-425-5 p, miR-1180-3 p, miR-122-5 p, miR-24-3 p and miR-4632-5 p can be regarded as a new potential biomarker panel for the diagnosis of EGC. The prediction and early diagnosis of EGC can be considerably facilitated by combining gastroscopy with the use of these miRNA biomarkers, thereby optimizing the strategy for effective detection of EGC. Nevertheless, larger-scale human experiments are still required to confirm our findings. | Xiao-Liang Zhu Long-Fei Ren Hai-Ping Wang Zhong-Tian Bai Lei Zhang Wen-Bo Meng Ke-Xiang Zhu Fang-Hui Ding Long Miao Jun Yan Yan-Ping Wang Yu-Qin Liu Wen-Ce Zhou Xun Li | 2019 | World Journal of Gastroenterology2019，25，13： | 11 |
| 17 | The Frattini Subalgebra of n-Lie Algebras显示文摘在这篇论文,我们主要学习 n 躺着代数学的 Frattini subalgebraand Jacobson 基的结构的观点。在 Frattini 潜水艇代数学和 Jacobson 基之间的 Frattini 潜水艇代数学和关系上的性质被学习。而且,当 n 躺着代数学是强壮的时,我们也学习他们半简单,可解决的κ - 和无有势力。 | Rui Pu BAI Liang Yun CHEN Dao Ji MENG | 2007 | Acta Mathematica Sinica,English Series2007，23，5： | 10 |
| 18 | An efficient adaptive finite element method algorithm with mass conservation for analysis of liquid face seals显示文摘研究目的:为液体润滑端面密封空化问题的数值模拟提供一种高效精确的计算方法。创新要点:1.采用流线迎风SUPG有限元法求解具有对流扩散特征的控制方程;2.针对未知量的互补关系提出了一种高效的数值迭代技术;3.提供了针对润滑液膜空化问题的网格自适应技术。重要结论:1.相比文献算法,本文算法具有计算速度快,计算精度高的优点;2.空化区域和大压力梯度区域的计算网格得到加密。 | Xiayg-kai MENG Shao-xian BAI Xu-dong PENG | 2014 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2014，15，3： | 9 |
| 19 | Climatology of tropical cyclone tornadoes in China from 2006 to 2018显示文摘We surveyed the occurrence of tropical cyclone(TC) tornadoes in China from 2006 to 2018. There were 64 cataloged TC tornadoes, with an average of five per year. About one-third of the landfalling TCs in China were tornadic. Consistent with previous studies, TC tornadoes preferentially formed in the afternoon shortly before and within about 36 h after landfall of the TCs. These tornadoes mainly occurred in coastal areas with relatively flat terrains. The maximum number of TC tornadoes occurred in Jiangsu and Guangdong provinces. Most of the TC tornadoes were spawned within 500 km of the TC center. Two notable characteristics were found:(1) TC tornadoes in China mainly occurred in the northeast quadrant(Earth-relative coordinates) rather than the right-front quadrant(TC motion-relative coordinates) of the parent TC circulation;and(2) most tornadoes were produced by TCs with a relatively weak intensity(tropical depressions/storms), in contrast with the United States where most tornadoes are associated with stronger TCs. Further analyses showed that TC tornadoes in China tend to be spawned in an environment with large low-level storm relative helicity and large convective available potential energy taking entrainment effects into account. TC tornadoes were particularly active in 2018, with 24 reported tornadoes accounting for 37.5% of the total surveyed samples. The first recorded tornado outbreak in the modern history of China occurred in the envelope of TC Yagi(2018), in which 11 tornadoes were reported in association with significant midlevel intrusions of dry air and the interaction of Yagi with an approaching midlatitude midlevel trough. | Lanqiang BAI Zhiyong MENG Kenta SUEKI Guixing CHEN Ruilin ZHOU | 2020 | Science China Earth Sciences2020，63，1： | 9 |
| 20 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
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