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| 1 | PacBio Sequencing and Its Applications显示文摘Single-molecule, real-time sequencing developed by Pacific Bio Sciences offers longer read lengths than the second-generation sequencing(SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research. The highly-contiguous de novo assemblies using Pac Bio sequencing can close gaps in current reference assemblies and characterize structural variation(SV) in personal genomes. With longer reads, we can sequence through extended repetitive regions and detect mutations, many of which are associated with diseases. Moreover, Pac Bio transcriptome sequencing is advantageous for the identification of gene isoforms and facilitates reliable discoveries of novel genes and novel isoforms of annotated genes,due to its ability to sequence full-length transcripts or fragments with significant lengths. Additionally, Pac Bio's sequencing technique provides information that is useful for the direct detection of base modifications, such as methylation. In addition to using Pac Bio sequencing alone, many hybrid sequencing strategies have been developed to make use of more accurate short reads in conjunction with Pac Bio long reads. In general, hybrid sequencing strategies are more affordable and scalable especially for small-size laboratories than using Pac Bio Sequencing alone. The advent of Pac Bio sequencing has made available much information that could not be obtained via SGS alone. | Anthony Rhoads Kin Fai Au | 2015 | Genomics, Proteomics & Bioinformatics2015，13，5： | 107 |
| 2 | Acute effects of rotavirus and malnutrition on intestinal barrier function in neonatal piglets显示文摘AIM: To investigate the effect of protein-energy malnutrition on intestinal barrier function during rotavirus enteritis in a piglet model.METHODS: Newborn piglets were allotted at day 4 of age to the following treatments:(1) full-strength formula(FSF)/noninfected;(2) FSF/rotavirus infected;(3) half-strength formula(HSF)/noninfected;or(4) HSF/rotavirus infected.After one day of adjustment to the feeding rates,pigs were infected with rotavirus and acute effects on growth and diarrhea were monitored for 3 d and jejunal samples were collected for Ussingchamber analyses.RESULTS: Piglets that were malnourished or infected had lower body weights on days 2 and 3 post-infection(P < 0.05).Three days post-infection,marked diarrhea and weight loss were accompanied by sharp reductions in villus height(59%) and lactase activity(91%) and increased crypt depth(21%) in infected compared with non-infected pigs(P < 0.05).Malnutrition also increased crypt depth(21%) compared to full-fed piglets.Villus:crypt ratio was reduced(67%) with viral infection.There was a trend for reduction in transepithelial electrical resistance with rotavirus infection and malnutrition(P = 0.1).3H-mannitol flux was significantly increased(50%;P < 0.001) in rotavirus-infected piglets compared to non-infected piglets,but there was no effect of nutritional status.Furthermore,rotavirus infection reduced localization of the tight junction protein,occludin,in the cell membrane and increased localization in the cytosol.CONCLUSION: Overall,malnutrition had no additive effects to rotavirus infection on intestinal barrier function at day 3 post-infection in a neonatal piglet model. | Sheila K Jacobi Adam J Moeser Anthony T Blikslager J Marc Rhoads Benjamin A Corl Robert J Harrell Jack Odle | 2013 | World Journal of Gastroenterology2013，19，31： | 4 |
| 3 | Bovine immunoglobulin protein isolates for the nutritional management of enteropathy显示文摘The gastrointestinal tract is responsible for a multitude of digestive and immune functions which depend upon the balanced interaction of the intestinal microbiota, diet, gut barrier function, and mucosal immune response. Disruptions in one or more of these factors can lead to intestinal disorders or enteropathies which are characterized by intestinal inflammation, increased gut permeability, and reduced capacity to absorb nutrients. Enteropathy is frequently associated with human immunodeficiency virus(HIV) infection, inflammatory bowel disease, autoimmune enteropathy, radiation enteritis, and irritable bowel syndrome(IBS), where pathologic changes in the intestinal tract lead to abdominal discomfort, bloating, abnormal bowel function(e.g., diarrhea, urgency, constipation and malabsorption). Unfortunately, effective therapies for the management ofenteropathy and restoring intestinal health are still not available. An accumulating body of preclinical studies has demonstrated that oral administration of plasmaor serum-derived protein concentrates containing high levels of immunoglobulins can improve weight, normalize gut barrier function, and reduce the severity of enteropathy in animal models. Recent studies in humans, using serum-derived bovine immunoglobulin/protein isolate, demonstrate that such protein preparations are safe and improve symptoms, nutritional status, and various biomarkers associated with enteropathy. Benefits have been shown in patients with HIV infection or diarrhea-predominant IBS. This review summarizes preclinical and clinical studies with plasma/serum protein concentrates and describes the effects on host nutrition, intestinal function, and markers of intestinal inflammation. It supports the concept that immunoglobulin-containing protein preparations may offer a new strategy for restoring functional homeostasis in the intestinal tract of patients with enteropathy. | Bryon W Petschow Anthony T Blikslager Eric M Weaver Joy M Campbell Javier Polo Audrey L Shaw Bruce P Burnett Gerald L Klein J Marc Rhoads | 2014 | World Journal of Gastroenterology2014，20，33： | 2 |
| 4 | Gadolinium delayed enhancement cardiovascular magnetic resonance correlates with clinical measures of myocardial infarction显示文摘 | W.Patricia Ingkanisorn Kenneth L Rhoads Anthony H Aletras Peter Kellman Andrew E Arai | 2004 | Journal of the American College of Cardiology2004，，12： | 1 |
| 5 | The Roles of Social Support and Self-Efficacy in Physical Health’s Impact on Depressive and Anxiety Symptoms in Older Adults显示文摘 | Amber L. Paukert Jeremy W. Pettit Mark E. Kunik Nancy Wilson Diane M. Novy Howard M. Rhoades Anthony J. Greisinger Oscar A. Wehmanen Melinda A. Stanley | 2010 | Journal of Clinical Psychology in Medical Settings2010，，4： | 1 |
| 6 | Effect of Glucagon-like Peptide-2 (GLP-2) on Diurnal SGLT1 Expression显示文摘 | Anthony P. Ramsanahie Urs V. Berger Michael J. Zinner Edward E. Whang David B. Rhoads Stanley W. Ashley | 2004 | Digestive Diseases and Sciences (-)2004，，11： | 1 |
| 7 | Is reperfusion injury an important cause of mucosal damage after porcine intestinal ischemia?显示文摘 | Anthony T Blikslager Malcolm C Roberts J.Marc Rhoads Robert A Argenzio | 1997 | Surgery1997，，5： | 1 |
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