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| 1 | PacBio Sequencing and Its Applications显示文摘Single-molecule, real-time sequencing developed by Pacific Bio Sciences offers longer read lengths than the second-generation sequencing(SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research. The highly-contiguous de novo assemblies using Pac Bio sequencing can close gaps in current reference assemblies and characterize structural variation(SV) in personal genomes. With longer reads, we can sequence through extended repetitive regions and detect mutations, many of which are associated with diseases. Moreover, Pac Bio transcriptome sequencing is advantageous for the identification of gene isoforms and facilitates reliable discoveries of novel genes and novel isoforms of annotated genes,due to its ability to sequence full-length transcripts or fragments with significant lengths. Additionally, Pac Bio's sequencing technique provides information that is useful for the direct detection of base modifications, such as methylation. In addition to using Pac Bio sequencing alone, many hybrid sequencing strategies have been developed to make use of more accurate short reads in conjunction with Pac Bio long reads. In general, hybrid sequencing strategies are more affordable and scalable especially for small-size laboratories than using Pac Bio Sequencing alone. The advent of Pac Bio sequencing has made available much information that could not be obtained via SGS alone. | Anthony Rhoads Kin Fai Au | 2015 | Genomics, Proteomics & Bioinformatics2015，13，5： | 107 |
| 2 | Can probiotics benefit children with autism spectrum disorders?显示文摘Children with autism are commonly affected by gastrointestinal problems such as abdominal pain, constipation and diarrhea. In recent years, there has been a growing interest in the use of probiotics in this population, as it hypothetically may help to improve bowel habits and the behavioral and social functioning of these individuals. The gut microbiome plays an important role in the pathophysiology of organic as well as functional gastrointestinal disorders. Microbial modification with the use of antibiotics, probiotics, and fecal transplantation have been effective in the treatment of conditions such as recurrent Clostridium difficile infection, pouchitis, and irritable bowel syndrome. The present review presents a number of reported clinical, immunological and microbiomerelated changes seen in children with autism compared to normally developed children. It also discusses gut inflammation, permeability concerns, and absorption abnormalities that may contribute to these problems. Most importantly, it discusses evidence, from human and animal studies, of a potential role of probiotics in the treatment of gastrointestinal symptoms in children with autism. | fernando Navarro Yuying Liu Jon Marc Rhoads | 2016 | World Journal of Gastroenterology2016，22，46： | 4 |
| 3 | Acute effects of rotavirus and malnutrition on intestinal barrier function in neonatal piglets显示文摘AIM: To investigate the effect of protein-energy malnutrition on intestinal barrier function during rotavirus enteritis in a piglet model.METHODS: Newborn piglets were allotted at day 4 of age to the following treatments:(1) full-strength formula(FSF)/noninfected;(2) FSF/rotavirus infected;(3) half-strength formula(HSF)/noninfected;or(4) HSF/rotavirus infected.After one day of adjustment to the feeding rates,pigs were infected with rotavirus and acute effects on growth and diarrhea were monitored for 3 d and jejunal samples were collected for Ussingchamber analyses.RESULTS: Piglets that were malnourished or infected had lower body weights on days 2 and 3 post-infection(P < 0.05).Three days post-infection,marked diarrhea and weight loss were accompanied by sharp reductions in villus height(59%) and lactase activity(91%) and increased crypt depth(21%) in infected compared with non-infected pigs(P < 0.05).Malnutrition also increased crypt depth(21%) compared to full-fed piglets.Villus:crypt ratio was reduced(67%) with viral infection.There was a trend for reduction in transepithelial electrical resistance with rotavirus infection and malnutrition(P = 0.1).3H-mannitol flux was significantly increased(50%;P < 0.001) in rotavirus-infected piglets compared to non-infected piglets,but there was no effect of nutritional status.Furthermore,rotavirus infection reduced localization of the tight junction protein,occludin,in the cell membrane and increased localization in the cytosol.CONCLUSION: Overall,malnutrition had no additive effects to rotavirus infection on intestinal barrier function at day 3 post-infection in a neonatal piglet model. | Sheila K Jacobi Adam J Moeser Anthony T Blikslager J Marc Rhoads Benjamin A Corl Robert J Harrell Jack Odle | 2013 | World Journal of Gastroenterology2013，19，31： | 4 |
| 4 | 谷氨酰胺、精氨酸和亮氨酸在肠道中的信号传导(一)显示文摘动植物蛋白中谷氨酰胺和亮氨酸含量丰富且较稳定,而食物和动物体液中精氨酸含量差异较大。除在蛋白质合成中起作用外,这三种氨基酸可独自激活信号传导通路以促进蛋白质的合成,并可能抑制肠上皮细胞自噬介导的蛋白质降解。此外,谷氨酰胺和精氨酸可分别激活有丝分裂原活化蛋白激酶通路和哺乳动物雷帕霉素靶蛋白(mTOR)/p70(s6)激酶通路,从而加强粘膜细胞的迁移和修复。利用一氧化氮依赖型cGMP的信号级联,精氨酸可调节肠道多种生理活动,这有利于细胞的生存和维持动态平衡。动物体内外试验显示,谷氨酰胺和精氨酸可促进细胞增殖,并可针对养分损失、氧化损伤、应激和免疫应激来发挥不同的细胞保护作用。此外,一氧化氮存在时亮氨酸可加强肠道细胞的迁移。因此,通过细胞信号传导机制,精氨酸、谷氨酰胺和亮氨酸在肠道的生长发育和功能完善过程中发挥了关键作用。 | J.Marc Rhoads Guoyao Wu 邓会玲 | 2011 | 饲料与畜牧(新饲料)2011，，9： | 3 |
| 5 | "LOCK"ing up allergic responses with a Polish probiotic显示文摘 | Yuying Liu Marc Rhoads | 2016 | Cellular & Molecular Immunology2016，13，2： | 3 |
| 6 | 非甾体抗炎药应用中,环氧合酶2、不对称二甲基精氨酸与心血管危害的关系显示文摘已有大规模、安慰剂对照试验确定了非甾体类抗炎药(nonsteroidal anti—inflammatory drugs,NSAIDs)具有心血管危害性:这归因于环氧合酶2(cyclooxygenase,COX-2)的心脏保护产物受到抑制,尤其是前列环素(prostacyclin,PG12)。NSAID降低心脏保护作用的另一种机制可能是通过增强肾脏中甲基精氨酸的形成来限制一氧化氮在整个脉管系统中的作用。 | Ricciotti E Castro C Tang SY Briggs WTE West JA Malik D Rhoades SD Meng H Li X Lahens NF Sparks JA Karlson EW Weljie AM Griffin JL FitzGerald GA6 刘青 叶鹏 | 2018 | 中华高血压杂志2018，26，9： | 3 |
| 7 | 热应激和低营养摄入对猪肠道完整性和功能的影响显示文摘热应激会损害肠道完整性,并诱发肠渗漏。因此,本试验研究了直接或间接热应激(通过减少采食量)对生长猪肠道通透性的影响。选用体重(43±4)kg的杂交后备母猪(n=48),分别安置在以下控制环境中:热中性(TN)环境(20℃,湿度35%~50%,自由采食)、热应激(HS)环境(35℃,湿度20%~35%,自由采食)、热中性环境配对饲喂(PFTN,以消除不同采食量的交互影响)。第1、3、7天使猪只安乐死,采集的空肠样品被安装到尤式灌流室,测定跨膜电阻抗值(TER)和异硫氰基荧光标记的脂多糖(LPS)渗透性(APP,表示为表观渗透系数)。此外,评估肠道完整性以及应激基因和蛋白标志物。结果表明,不考虑热应激天数,HS组与TN组猪相比,血浆内毒素水平增加45%(P〈0.05),空肠TER降低30%(P〈0.05),LPS渗透率增加2倍(P〈0.01)。此外,与PFTN组相比,HS组第7天的猪LPS渗透性有增加的趋势(P=0.06)。整个试验阶段HS组溶菌酶和碱性磷酸酶活性下降,分别下降46%和59%(P〈0.05);然而,3 d和7 d空肠免疫细胞标记物——髓过氧化物酶活性增加(P〈0.05)。由此可见,热应激和减少采食量都会降低肠道完整性,并增加内毒素渗透性。推测这可能会导致炎症加重,在炎热的夏季可能是导致猪生长性能降低的原因。 | Pearce S C Mani V Weber T E Rhoads R P Patience J F Baumgard L H Gabler N K | 2016 | 中国饲料2016，，3： | 3 |
| 8 | Bovine immunoglobulin protein isolates for the nutritional management of enteropathy显示文摘The gastrointestinal tract is responsible for a multitude of digestive and immune functions which depend upon the balanced interaction of the intestinal microbiota, diet, gut barrier function, and mucosal immune response. Disruptions in one or more of these factors can lead to intestinal disorders or enteropathies which are characterized by intestinal inflammation, increased gut permeability, and reduced capacity to absorb nutrients. Enteropathy is frequently associated with human immunodeficiency virus(HIV) infection, inflammatory bowel disease, autoimmune enteropathy, radiation enteritis, and irritable bowel syndrome(IBS), where pathologic changes in the intestinal tract lead to abdominal discomfort, bloating, abnormal bowel function(e.g., diarrhea, urgency, constipation and malabsorption). Unfortunately, effective therapies for the management ofenteropathy and restoring intestinal health are still not available. An accumulating body of preclinical studies has demonstrated that oral administration of plasmaor serum-derived protein concentrates containing high levels of immunoglobulins can improve weight, normalize gut barrier function, and reduce the severity of enteropathy in animal models. Recent studies in humans, using serum-derived bovine immunoglobulin/protein isolate, demonstrate that such protein preparations are safe and improve symptoms, nutritional status, and various biomarkers associated with enteropathy. Benefits have been shown in patients with HIV infection or diarrhea-predominant IBS. This review summarizes preclinical and clinical studies with plasma/serum protein concentrates and describes the effects on host nutrition, intestinal function, and markers of intestinal inflammation. It supports the concept that immunoglobulin-containing protein preparations may offer a new strategy for restoring functional homeostasis in the intestinal tract of patients with enteropathy. | Bryon W Petschow Anthony T Blikslager Eric M Weaver Joy M Campbell Javier Polo Audrey L Shaw Bruce P Burnett Gerald L Klein J Marc Rhoads | 2014 | World Journal of Gastroenterology2014，20，33： | 2 |
| 9 | Effects of heat stress and plane of nutrition on lactating Holstein cows: I. Production, metabolism, and aspects of circulating somatotropin 1显示文摘 | M.L. Rhoads R.P. Rhoads M.J. VanBaale R.J. Collier S.R. Sanders W.J. Weber B.A. Crooker L.H. Baumgard | 2009 | Journal of Dairy Science2009，，5： | 2 |
| 10 | 热应激对猪生产和代谢的影响显示文摘文章综述了热应激对猪的生产和代谢的影响,以及热应激对猪肠道健康的影响。并从饲养管理和营养措施两方面阐述了应对热应激的措施。其中营养措施包括:锌、乳制品、谷氨酰胺、抗氧化剂、地塞米松、甜菜碱的使用。 | M.V. Sanz-Fernandez S.K. Stoakes J.S. Johnson M. Abuajamieh J.T. Seibert S.C. Pearce N.K. Gabler R.P. Rhoads L.H. Baumgard | 2014 | 饲料工业2014，35，10： | 2 |
| 11 | Assessment of sala tolerance in rice cultivars in response to salinity problems in California 显示文摘 | Shannon M C Rhoades J D Draper J H | 1998 | Crop Science1998，38，2： | 2 |
| 12 | Prediction of plant microRNA targets 显示文摘 | Rhoades M W Reinhart B J Lim L P | 2002 | Cell2002，110，4： | 1 |
| 13 | Time Domain Reflectometry : Simultaneous Measurement of Soil Water Content and Electrical Conductivity with a Single Probe 显示文摘 | DALTON F N HERKELRATH W N RAWLINS D S RHOADES J D | 1984 | Science1984，224，： | 1 |
| 14 | Hydrogen Peroxide stimulates extracellular signal-- regulated protein kinases in pulmonary arterial smooth muscle cells显示文摘 | Jin N Liu Y Rhoades RA | 1998 | AmJ RespirCell Mol Biol1998，19，2： | 1 |
| 15 | Fixed point theory in symmetric spaces with applications to probabilistic spaces显示文摘 | Hicks T L Rhoades B E | 1999 | Nonl Anal1999，36，3： | 1 |
| 16 | Capitalism, Academic Style, and Shared Governance 显示文摘 | RHOADES G | 2005 | Acdeme2005，2005，： | 1 |
| 17 | En- terovirus infections of the central nervous system 显示文摘 | Rhoades RE Tabor-Godwin JM Tsueng G Feuer R | 2011 | Virol- ogy2011，411，2： | 1 |
| 18 | Perceived Organ-izational Support: A Review of the Literature 显示文摘 | RHOADES L EISENBERGER R | 2002 | Journal of Applied Psychology2002，87，4： | 1 |
| 19 | Common fixed point theorems for three mappings under a weak commutativity condition显示文摘 | Rhoades B E Sessa S | 1986 | Indian Journal ofPure and Applied Mathematics1986，，1： | 1 |
| 20 | Perceived Organizational Support:A Review of the Literature显示文摘 | Linda Rhoades Robert Eisenberger | | 0，，04： | 1 |
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