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| 1 | Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease显示文摘AIM: To establish a mouse model of alcohol-driven hepatocellular carcinoma(HCC) that develops in livers with alcoholic liver disease(ALD).METHODS: Adult C57BL/6 male mice received multiple doses of chemical carcinogen diethyl nitrosamine(DEN) followed by 7 wk of 4% Lieber-De Carli diet. Serum alanine aminotransferase(ALT), alpha fetoprotein(AFP) and liver Cyp2e1 were assessed. Expression of F4/80, CD68 for macrophages and Ly6 G, MPO, E-selectin for neutrophils was measured. Macrophage polarization was determined by IL-1β/i NOS(M1) and Arg-1/IL-10/CD163/CD206(M2) expression. Liver steatosis and fibrosis were measured by oil-red-O and Sirius red staining respectively. HCC development was monitored by magnetic resonance imaging, confirmed by histology. Cellular proliferation was assessed by proliferating cell nuclear antigen(PCNA).RESULTS: Alcohol-DEN mice showed higher ALTs than pair fed- DEN mice throughout the alcohol feeding without weight gain. Alcohol feeding resulted in increased ALT, liver steatosis and inflammation compared to pair-fed controls. Alcohol-DEN mice had reduced steatosis and increased fibrosis indicatingadvanced liver disease. Molecular characterization showed high estlevels of both neutrophil and macrophage markers in alcohol-DEN livers. Importantly, M 2 macrophages were edominantly higher in alcohol-DEN livers. Magnetic resonance imaging revealed increased numbers of intrahepatic cysts and liver histology confirmed the presence of early HCC in alcohol-DEN mice compared to al l other groups. This correlated with increased serum alphafetoprotein, a marker of HCC, in alcohol-DEN mice. PCNA immunostaining revealed significantly increased hepatocyte proliferation in livers from alcohol-DEN compared to pair fed-DEN or alcohol-fed mice.CONCLUSION: We describe a new 12-wk HCC model in adult mice that develops in livers with alcoholic hepatitis and defines ALD as co-factor in HCC. | Aditya Ambade Abhishek Satishchandran Benedek Gyongyosi Patrick Lowe Gyongyi Szabo | 2016 | World Journal of Gastroenterology2016，22，16： | 4 |
| 2 | Urinary n - acetyl beta glucosaminidase and gamma glutamyl transfemse as early markers of diabetic nephropathy 显示文摘 | Vivek Ambade Parduman Singh BL Somani | 2006 | Indian Journal of Clinical Biochemistry2006，21，2： | 1 |
| 3 | Controlling the degree of branching in vinyl polymerization显示文摘 | Ambade A V Kumar A | 2000 | Prog Polym Sci2000，25，： | 1 |
| 4 | An essential role for MCP-1 in alcoholic liver injury: regulation of pro-inflammatory cytokines and hepatic steatosis显示文摘 | Pranoti Mandrekar Aditya Ambade Arlene Lim | 2011 | Hepatology2011，54，6： | 1 |
| 5 | Moderate alcohol induces stress proteins HSF1 and HSP70 and inhibits proinflammatory cytokines resulting in endotoxin tolerance 显示文摘 | Muralidharan S Ambade A Fulham MA | 2014 | J Immunol2014，193，4： | 1 |
| 6 | Controlling the degree of branching in vinyl polymerization显示文摘 | Ambade A V Kumar A | 2000 | Prog Polym Sci2000，25，： | 1 |
| 7 | Urinary N-acetyl beta glucosaminidase and gamma glutamyl transferase as early markers of diabetic nephropathy.显示文摘 | Vivek Ambade Parduman Sing B L Somani Dashrath Basanna | 2006 | Indian Journal of Clinical Biochemistry2006，，2： | 1 |
| 8 | Effect of sui- cide gene therapy in combination with immunotherapy on antitumour immune response tumour regression in a xenograft mouse model for head neck squamous cell carcinoma显示文摘 | Ambade A V Joshi G V Mulherkar R | 2010 | Indian J Med Res2010，132，： | 1 |
| 9 | Moderate alcohol induces stress proteins HSF1and hsp70and inhibits proinflammatory cytokines resulting in endotoxin tolerance显示文摘 | Muralidharan S Ambade A Fulham MA | 2014 | J Immunol2014，193，4： | 1 |
| 10 | Controlling the degree of branching in vinyl polymerization 显示文摘 | AMBADE A V KUMAR A | 2000 | Prog Polym Sci2000，25，8： | 1 |
| 11 | Oxidative stress and inflammation:essential partners in alcoholic liver disease显示文摘 | Ambade A Mandrekar P | 2012 | Int J Hepatol2012，2012，： | 1 |
| 12 | Contact angle measurement : A preliminary diagnostic method for evalua- ting the performance of ZnO platelet-based dye-sensitized solar cells显示文摘 | Ambade B Mane R S Ghule A V | 2009 | Scripta Materialia2009，61，1： | 1 |
| 13 | 显示文摘 | Ambade A V Kumar A | 2001 | J Polym Sci Part A:Polym Chem2001，39，9： | 1 |
| 14 | Development and in vitro evaluation of an oral floating matrix tablet formulation of diltiazem hydrochloride显示文摘 | GAMBHIRE M N AMBADE K W KURMI S D | 2007 | AAPS Pharm Sci Tech2007，8，3： | 1 |
| 15 | Adoptive T cell transfer augments IL-2 mediated tumour regression in a HNSCC xenograft nude mouse model显示文摘 | Aditya Ambade Rita Mulherkar | 2008 | Cancer Letters2008，272，2： | 1 |
| 16 | Applications of microem-ulsion based drug delivery system显示文摘 | Jadhav K R Shaikh I M Ambade K W | 2006 | Current Drug Delivery2006，12，3： | 1 |
| 17 | An essential role for monocyte chemoattractant protein-1 in alcoholic liver injury:regulation of proinflammatory cytokines and hepatic steatosis in mice显示文摘 | Mandrekar P Ambade A Lim A | 2011 | Hepatology2011，54，6： | 1 |
| 18 | Supramolecular ABCtriblock copolymers via one-pot, orthogonal self-assembly 显示文摘 | YANG S K AMBADE A V WECK M | 2010 | Journal of the American Chemical Society2010，132，： | 1 |
| 19 | Pharmacological Inhibition of Heat Shock Protein 90 Alleviates Steatosis and Macrophage Activation in Murine Acute and Chronic Alcoholic Liver Injury显示文摘 | Aditya Ambade Donna Catalano Arlene Lim Andre Kopoyan Scott A. Shaffer Pranoti Mandrekar | 2014 | Journal of Hepatology2014，，： | 1 |
| 20 | Applications of microemulsion based drug delivery system 显示文摘 | Jadhav K R Shaikh I M Ambade K W | 2006 | Curr Drug Deliv2006，3，3： | 1 |
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