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| 1 | Brazilian biosafety law and the new breeding technologies显示文摘Globally,the area of land cultivated with genetically modified(GM)crops has increased a thousand-fold over the last two decades.Although this technology has become important for food production,the regulatory frameworks that underpin these outcomes are based on a list of requirements for a risk assessment that differ from country to country.In recent years,policymakers have had the opportunity to learn from the controversies over transgenics to create effective regulatory milestones for emerging technologies,allowing them to reach their potential for a more sustainable agriculture,ensuring food security.In Brazil,Law No.11.105 of 24 March 2005 established a framework with four main organizations responsible for risk assessment and management.However,most of new breeding technologies did not exist at that time and were not considered in this law.In2016,Normative Resolution No.16 of the National Biosafety Technical Commission(CTNBio)was established to address this gap based on the evaluation of the products obtained through these techniques(termed Innovative Precision Improvement Techniques in the resolution),in a case-by-case consultation system.Briefly,if the product is designated to be a GM,the developer will have to go through the biosafety requirements and will be approved only after CTNBio risk assessment.If the product is designated not to be GM(for the purposes of the legislation),then it can be registered using the existing procedures.Currently,152 GM products are commercially approved in Brazil.In 2018,CTNBio assessed the first consultation on commercial release of plants generated using the new breeding technologies and has subsequently approved six products.It is expected that many institutions would be able to participate in Brazilian and world markets,developing and introducing new biotechnological solutions and products through a more sustainable approach and without facing public disapproval,a common issue for GM crops. | Alexandre Lima NEPOMUCENO Renata FUGANTI-PAGLIARINI Maria Sueli Soares FELIPE Hugo Bruno Correa MOLINARI Edivaldo Domingues VELINI Eduardo Romano de Campos PINTO Maria Lucia Zaidan DAGLI Galdino ANDRADE FILHO Patricia Machado Bueno FERNANDES | 2020 | Frontiers of Agricultural Science and Engineering2020，7，2： | 5 |
| 2 | Clinical hematological and biochemical parameters in Swiss,BALB/c,C57BL/6 and B6D2F1 Mus musculus显示文摘Background:Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions.Various lineages of mice have been used to investigate diseases and new therapeutic strategies,and,consequently,hematological and biochemical tests in these laboratory animals are essential to validate scientific studies.Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice.Methods:We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss(heterogeneous),BALB/c and C57BL/6(isogenic),and B6D2F1(hybrid),totaling 160 mice.Analysis were standardized using the systems pocH-100iV Diff™for 19 hematological parameters and VITROS®350 for 12 biochemical parameters.Results:Results are shown as means and standard deviation,grouped by lineage and genre.Comparing the values obtained in this study with the values from previous studies,some variations were detected,which could be explained by differences in methodologies or individual variability.Conclusion:Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary,and emphasises the importance of considering variations influenced by gender,lineage and genotype in the choice of the best experimental model. | Giorgio Silva-Santana Juliet Cunha Bax Débora Cristina Silva Fernandes Daniela Tendler Leibel Bacellar Cleber Hooper Alexandre Alves Souza Oliveira Dias Cristina Barbosa Silva Aline Moreira de Souza Simone Ramos Ricardo Alexandre Santos Thainara Ramos Pinto Mariana Antunes Ramão Ana Luíza Mattos-Guaraldi | 2020 | Animal Models and Experimental Medicine2020，3，4： | 2 |
| 3 | Acute cholestatic hepatitis caused by amoxicillin/ clavulanate显示文摘Amoxicillin/clavulanate is a synthetic penicillin that is currently commonly used,especially for the treatment of respiratory and cutaneous infections.In general,it is a well-tolerated oral antibiotic.However,amoxicillin/clavulanate can cause adverse effects,mainly cutaneous,gastrointestinal,hepatic and hematologic,in some cases.Presented here is a case report of a 63-yearold male patient who developed cholestatic hepatitis after recent use of amoxicillin/clavulanate.After 6 wk of prolonged use of the drug,he began to show signs of cholestatic icterus and developed severe hyperbilirubinemia(total bilirubin>300 mg/L).Diagnostic investigation was conducted by ultrasonography of the upper abdomen,serum tests for infection history,laboratory screening of autoimmune diseases,nuclear magnetic resonance(NMR)of the abdomen with bile duct-NMR and transcutaneous liver biopsy guided by ultrasound.The duration of disease was approximately 4 mo,with complete resolution of symptoms and laboratory changes at the end of that time period.Specific treatment was not instituted,only a combination of anti-emetic(metoclopramide)and cholestyramine for pruritus. | Daniel Oliveira Beraldo Joanderson Fernandes Melo Alexandre Vidal Bonfim Andrei Alkmim Teixeira Ricardo Alkmim Teixeira André Loyola Duarte | 2013 | World Journal of Gastroenterology2013，19，46： | 2 |
| 4 | Biochar Increases Diuron Sorption and Reduces the Potential Contamination of Subsurface Water with Diuron in a Sandy Soil显示文摘The herbicide diuron is widely used in agricultural areas in Brazil, whereas it has high potential for subsurface water contamination due to its physicochemical characteristics. Recent studies have demonstrated the potential of biochar as a sorbent and possible pesticide leaching mitigation. The objective of this study was to investigate the long-term effect of biochar application on the kinetics of sorption and desorption of diuron in a Cerrado Haplic Plinthosol. Samples were collected in an experiment conducted in the field in a randomized block design consisting of the combination of two levels of fertilizer application (0 and 300 kg ha −1 of 5-25-15 formula of NPK fertilizers) and three doses of biochar (0, 16, and 32 Mg ha −1 ). The Freundlich isotherm accurately described the sorption of diuron in all treatments. Biochar application increased the sorption and reduced the desorption of diuron. This effect was attributed to the contribution of biochar to total organic carbon (C) and C in the humin fraction and to the increase in the reactivity of the humic acid and humin fractions, which was significantly highly correlated with the sorption coefficient ( K f ). A positive correlation between the partition coefficient of organic C and K f confirmed the importance of the soil organic compartment for the sorption of diuron. The higher diuron sorption and lower diuron desorption capacities of sandy soils after biochar application could reduce the potential risk of diuron leaching and contamination of subsurface water. | Fabiano APETTER Tamara SFERREIRA Adilson PSINHORIN Larissa BLIMA Fernandes AALMEIDA Leandro PPACHECO Alexandre FSILVA | 2019 | Pedosphere2019，29，6： | 1 |
| 5 | Genetic polymorphisms involved in folate metabolism and concentrations of methylmalonic acid and folate on plasma homocysteine and risk of coronary artery disease显示文摘 | Patrícia Matos Biselli Alexandre Rodrigues Guerzoni Moacir Fernandes Godoy Marcos Nogueira Eberlin Renato Haddad Valdemir Melechco Carvalho Hélio Vannucchi érika Cristina Pavarino-Bertelli Eny Maria Goloni-Bertollo | 2010 | Journal of Thrombosis and Thrombolysis2010，，1： | 1 |
| 6 | Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential显示文摘Objective:To evaluate the antioxidant,antibacterial and bacterial cell agglutination activities of the hexane(Hex) and 70%ethanol(70%EtOH) extracts of two species of red seaweeds Pterocladiella capillacea(P.capillacea) and Osmundaria obtusiloba.Methods:In vitro antioxidant activity was determined by DPPH radical scavenging assay,ferric-reducing antioxidant power assay,ferrous ion chelating assay,β-carotene bleaching assay and total phenolic content quantification.Antimicrobial activity was tested using the method of disc diffusion on Mueller-Hinton medium.The ability of algal extracts to agglutinate bacterial cells was also tested.Results:The 70%EtOH extract of the two algae showed the highest values of total phenolic content compared to the Hex extract.The results of DPPH for both extracts(Hex,70%EtOH) of Osmundaria obtusiloba(43.46%and 99.47%) were higher than those of P.capillacea(33.04%and 40.81%) at a concentration of 1 000 μg/mL.As for the ferrous ion chelating,there was an opposite behavior,extracts of P.capillacea had a higher activity.The extracts showed a low ferric-reducing antioxidant power,with optical density ranging from0.054 to 0.180.Antioxidant activities of all extracts evaluated for β-carotene bleaching were above 40%.There was no antibacterial activity against bacterial strains tested.However,the extracts of both species were able to agglutinate bacterial Gram positive cells of Staphylococcus aureus and Gram negative cells of Escherichia coli,multidrug-resistant Salmonella and Vibrio harveyi.Conclusions This is the first report of the interaction between these algal extracts,rich in natural compounds with antioxidant potential,and Gram positive and Gram negative bacterial cells. | Daniel Barroso de Alencar Fátima Cristiane Teles de Carvalho Rosa Helena Rebouas Daniel Rodrigues dos Santos Kelma Maria dos Santos Pires-Cavalcante Rebeca Larangeira de Lima Bárbara Mendes Baracho Rayssa Mendes Bezerra Francisco Arnaldo Viana Regine Helena Silva dos Fernandes Vieira Alexandre Holanda Sampaio Oscarina Viana de Sousa Silvana Saker-Sampaio | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，4： | 1 |
| 7 | Genetic polymorphisms involved in folate metabolism and concentrations of methylmalonic acid and folate on plasma homocysteine and risk of coronary artery disease显示文摘 | Patrícia Matos Biselli Alexandre Rodrigues Guerzoni Moacir Fernandes Godoy Marcos Nogueira Eberlin Renato Haddad Valdemir Melechco Carvalho Hélio Vannucchi érika Cristina Pavarino-Bertelli Eny Maria Goloni-Bertollo | 2010 | Journal of Thrombosis and Thrombolysis2010，，1： | 1 |
| 8 | Modeling and Optimization of Lactic Acid Production using Cashew Apple Juice as Substrate显示文摘 | Alexandre A. Guilherme Mariana S. Silveira Claudia P. M. L. Fontes Sueli Rodrigues Fabiano A. N. Fernandes | 2012 | Food and Bioprocess Technology2012，，8： | 1 |
| 9 | Pre-Columbian Chagas disease in Brazil: Trypanosoma cruzi I in the archaeological remains of a human in Peruagu Valley, Minas Gerais, Brazil显示文摘 | Alexandre Fernandes Alena M Ifiiguez Valdirene S Lima | 2008 | Mem Inst Oswaldo Cruz Rio de Janeiro2008，103，5： | 1 |
| 10 | First-Line Paclitaxel and Carboplatin in Persistent/Recurrent or Advanced Cervical Cancer: A Retrospective Analysis of Patients Treated at Brazilian National Cancer Institute显示文摘 | álvaro Henrique Ingles Garces Paulo Alexandre Ribeiro Mora Flávia Vieira Guerra Alves Claudio Calazan do Carmo Rachele Grazziotin Anna Cristina Ferr?o Mangia Fernandes Angélica Nogueira-Rodrigues Andréia Cristina de Melo | 2013 | International Journal of Gynecological Cancer2013，，4： | 1 |
| 11 | Al/SiCP functionally graded metal-matrix compositesproduced by centrifugal casting : effect of particle grain size onreinforcement distribution 显示文摘 | Velhinho Alexandre Sequeira P D Fernandes Francisco ManuelBraz | 2003 | Materials Science Forum2003，，： | 1 |
| 12 | An Estimate of the Cost of Hepatitis C Treatment for the Brazilian Health System显示文摘 | Carine Raquel Blatt Noemia Liege Maria da Cunha Bernardo Junior Andre Rosa Fabiola Bagatini Rodrigo Fernandes Alexandre Giacomo Balbinotto Neto Uwe Siebert Mareni Rocha Farias | 2012 | Value in Health Regional Issues2012，，： | 1 |
| 13 | Vascular endothelial growth factor genetic variability and coronary artery disease in Brazilian population显示文摘 | Patrícia Matos Biselli Alexandre Rodrigues Guerzoni Moacir Fernandes Godoy érika Cristina Pavarino-Bertelli Eny Maria Goloni-Bertollo | 2008 | Heart and Vessels2008，，6： | 1 |
| 14 | River Network and the Risk of Flooding for the Railway and Subway Systems in the City of Sao Paulo, Brazil显示文摘 | Daniel Nery dos Santos Marcos Antonio Maia de Oliveira Alexandre Formigoni Joao Roberto Maiellaro Antonio Roberto Saad Marcelo Eloy Fernandes | 2016 | Journal of Geological Resource and Engineering2016，4，7： | 0 |
| 15 | Vitamin D deficiency,functional status,and balance in older adults with osteoarthritis显示文摘BACKGROUND Low vitamin D levels are associated with a more severe case of knee osteoarthritis(OA).However,there are few published reports concerning an association between vitamin D deficiency and functional status of individuals with OA and no reports about postural balance in this population.AIM To analyze the relationship between vitamin D deficiency and severity,functional status,and balance in elderly patients with OA.METHODS In this cross-sectional study,105 elderly patients with hip and knee OA were included.The severity was assessed by the Kellgren-Lawrence criteria.The functional status was assessed with the Lequesne index.Postural balance was assessed using a force platform,and center-of-pressure parameters(velocity at anteroposterior and mediolateral axis)were used as the balance outcomes.Serum 25(OH)vitamin D levels were measured using a chemiluminescence method.RESULTS Most of the patients(mean age:70.6±6.5 years)were female(n=78,74.3%).In the group with vitamin D deficiency,43 patients(56.6%)had severe OA,while 33 patients(43.4%)had mild or moderate OA(χ^(2) test,P=0.04).Patients with vitamin D deficiency showed a higher Lequesne index score(Mann-Whitney test,P=0.04),indicating a worse functional impairment when compared to individuals with normal vitamin D levels.Additionally,patients with vitamin D deficiency had worse postural balance according to the Mann-Whitney test(P=0.03).CONCLUSION Vitamin D deficiency is associated with worse severity,functional status,and postural balance in patients with OA. | Cláudia N Montemor Marcos Tadeu P Fernandes Audrey S Marquez Regina Célia Poli-Frederico Rubens Alexandre da Silva Karen B P Fernandes | 2021 | World Journal of Clinical Cases2021，9，31： | 0 |
| 16 | Oxidative stress and labile plasmatic iron in anemic patients following blood therapy显示文摘AIM: To determine the plasmatic iron content and evaluate the oxidative stress(OS) markers in subjects receiving blood therapy.METHODS: Thirty-nine individuals with unspecified anemia receiving blood transfusions and 15 healthy subjects were included in the study. Anemic subjects were divided into three subgrou P:(1) those that received up to five blood transfusions(n = 14);(2) those that received from five to ten transfusions(n = 11); and(3) those that received more than ten transfusions(n = 14). Blood samples were collected by venous arm puncture and stored in tubes containing heparin. The plasma and cells were separated by centrifugation and subsequently used for analyses. Statistical analyses were performed using Kruskal-Wallis analysis of variance followed by Dunn's multiple comparison tests when appropriate.RESULTS: The eletrophoretic hemoglobin profiles of the subjects included in this study indicated that no patients presented with hemoglobinopathy. Labile plasmatic iron, ferritin, protein carbonyl, thiobarbituric acidreactive substances(TBARS) and dichlorofluorescein diacetate oxidation were significantly higher(P < 0.05), whereas total thiol levels were significantly lower(P < 0.05) in transfused subjects compared to controls. Additionally, the activity of catalase, superoxide dismutase and glutathione peroxidase were significantly lower in the transfused subjects(P < 0.05). Antioxidant enzyme activities and total thiol levels were positively correlated(P < 0.05), and negatively correlated with the levels of protein carbonyl and TBARS(P < 0.05). In contrast, protein carbonyl and TBARS were positively correlated(P < 0.05). Altogether, these data confirm the involvement of OS in patients following therapy with repeated blood transfusions.CONCLUSION: Our data reveal that changes in OS markers are correlated with levels of labile plasmatic iron and ferritin and the number of transfusions. | Marília Sabo Fernandes Tatiana Tamborena Rissi Luisa Zuravski Juliana Mezzomo Carmen Regla Vargas Vanderlei Folmer Félix Alexandre Antunes Soares Vanusa Manfredini Mushtaq Ahmed Robson Luiz Puntel | 2014 | World Journal of Experimental Medicine2014，4，3： | 0 |
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