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| 1 | 广州小北路黑人聚居区社会空间分析显示文摘1978年改革开放带来中国城市的快速转型。近年来,针对经济转型背景下中国大城市的社会空间研究很多,但对在全球化影响下出现的跨国移民族裔聚居区的研究尚不多见。对广州黑人聚居区展开研究,以越秀区小北路为例,探讨其社会空间特征与演进机制。研究采用微观视角,针对典型案例进行问卷调查和半结构式访谈。研究表明,广州黑人聚居区与西方同类族裔社会区存在诸多差异。小北路黑人聚居区因全球化下新的'自下而上'的跨国经济联系而生,因广州城市的商贸文化、宗教历史、贸易网络和地理气候条件而兴,其跨国移民多为来自西非地区的族裔散居者/漂泊者(Diaspora),其人口构成异质多元,且流动性强。小北路的社会空间机制既包含被动隔离也包含主动聚居,其经济形态已经开始向'族裔聚居区经济'(ethnic enclave economy)发展。 | 李志刚 薛德升 Michael Lyons Alison Brown | 2008 | 地理学报2008，63，2： | 123 |
| 2 | A community-derived classification for extant lycophytes and ferns显示文摘发展史长通知了蕨类植物分类。当我们推断进化的树的能力改善了,针对认出生来的组的分类变得逐渐地预兆、稳定。这里,我们为 lycophytes 和蕨纲植物提供一个现代、全面分类,在下面,利用一条基于社区的途径类水平。我们 monophyly 用作主要标准让 taxa,而且目的识别保存两个广泛地被接受的存在 taxa 和界限并且与我们蕨类植物发展史的理解一致。总共,这个分类对待一在 337 个类, 51 个家庭, 14 目,和二个班估计了 11 916 种类。这个分类没在 lycophyte 和蕨纲植物上作为最后的词被打算分类,而是当前的假设的概括陈述,源于最好的可得到的数据并且在问题由熟悉植物的那些大多数塑造了。我们希望它将在蕨类植物上为最近的文学的那些想要的参考用作一个资源发展史和分类,为指导未来调查的一个框架,和推进讲话的刺激。 | Eric Schuettpelz Harald Schneider Alan R. Smith Peter Hovenkamp Jefferson Prado Germinal Rouhan Alexandre Salino Michael Sundue Thafs Elias Almeida Barbara Parris Emily B. Sessa Ashley R. Field Andre Luis de Gasper Carl J. Rothfels Michael D. Windham Marcus Lehnert Benjamin Dauphin Atsushi Ebihara Samuli Lehtonen Pedro Bond Schwartsburd Jordan Metzgar Li-Bing Zhang Li-Yaung Kuo Patrick J. Brownsey Masahiro Kato Marcelo Daniel Arana Francine C. Assis Michael S. Barker David S. Barrington Ho-Ming Chang Yi-Han Chang Yi-Shan Chao Cheng-Wei Chen De-Kui Chen Wen-Liang Chiou Vinicius Antonio de Oliveira Dittrich Yi-Fan Duan Jean-Yves Dubuisson Donald R. Farrar Susan Fawcett Jose Maria Gabriel y Galan Luiz Armando de Araujo Goes-Neto Jason R. Grant Amanda L. Grusz Christopher Haufler Warren Hauk Hai He Sabine Hennequin Regina Yoshie Hirai Layne Huiet Michael Kessler Petra Korall Paulo H. Labiak Anders Larsson Blanca Leen Chun-Xiang Li Fay-Wei Li Melanie Link-Perez Hong-Mei Liu Ngan Thi Lu Esteban I. Meza-Torres Xin-Yuan Miao Robbin Moran Claudine Massi Mynssens Nathalie Nagalingum Benjamin Ollgaard Alison M. Paul Jovani B. de S. Pereira Leon R. Perrie Monica Ponce Tom A. Ranker Christian Schulz Wataru Shinohara Alexander Shmakov Erin M. Sigel Filipe Soares de Souza Lana da Silva Sylvestre Weston Testo Luz Amparo Triana-Moreno Chie Tsutsumi Hanna Tuomisto IvAn A. Valdespino Alejandra Vasco Raquel Stauffer Viveros Alan Weakley Ran Wei Stina Weststrand Paul G. Wolf George Yatskievych Xiao-Gang Xu Yue-Hong Yan Liang Zhang Xian-Chun Zhang Xin-Mao Zhou | 2016 | Journal of Systematics and Evolution2016，54，6： | 44 |
| 3 | 拉米夫定治疗慢性乙型肝炎生存质量评价显示文摘目的 评价拉米夫定治疗慢性乙型肝炎生存质量的改善状况。方法 分别于拉米夫定治疗前、治疗半年、1年采用SF 3 6量表测定 2 44例病人的生存质量评分。结果 与治疗前相比 ,拉米夫定治疗半年及 1年后在躯体角色RP( 9.6)、社会功能SF( 8.2 )、情绪角色RE( 11.8)和活力VT( 6.2 ) 4个领域得分有显著提高 (P <0 .0 5 ) ,主要集中在心理状态评分MCS( 3 .6)。结论 拉米夫定治疗慢性乙型肝炎病人能有效改善病人生存质量。 | 姚光弼 Alison T M 黄瑛 籍纳新 杨莉 | 2003 | 肝脏2003，8，4： | 39 |
| 4 | 中文版Kogan老年态度量表的修订研究显示文摘目的翻译并修订Kogan老年态度量表(KAOP),并考察其心理测量学特征。方法使用翻译的中文版KAOP对465名注册护士进行问卷调查,检验其条目鉴别度、结构效度、信度等心理学测量学特征。结果因素分析共提取2个因子,累计方差贡献率为35.959%。内部一致性信度为0.788,消极分量表(OP-)的内部一致性信度为0.847,积极分量表(OP+)的内部一致性信度为0.804。重测信度为0.974。本研究修订的KAOP与原英文量表结构一致,内部一致性信度较好,重测信度高,跨时间稳定性较好。但与原量表相比,删除了部分条目,最终保留25个条目,使其更适应于目标人群。结论修订的KAOP量表具有较好的信效度,可用于评估护士对老年人的态度。 | 刘云娥 杨文丽 马艳林 Alison While 叶文琴 陈洁 | 2014 | 中华护理杂志2014，49，11： | 30 |
| 5 | 拉米夫定治疗慢性乙型肝炎病毒感染的经济效益显示文摘目的 评估慢性乙型肝炎及其并发症的经济负担和拉米夫定治疗的临床效果与经济效益。方法 根据慢性乙型肝炎、代偿性肝硬化、失代偿性肝硬化和肝癌病人的年直接医疗费用、直接非医疗费用以及间接费用计算其经济负担。同时根据亚洲拉米夫定多中心临床研究和 15个国家临床研究的综合数据来计算节省的费用。结果 不同肝病阶段的病人年经济负担不同 ,慢性乙型肝炎为 2 0 478元 ,代偿性肝硬化为 3 63 2 5元 ,失代偿性肝硬化为 3 675 9元 ,肝细胞肝癌为 3 82 69元。拉米夫定治疗 1年后 ,使医疗费用得到部分节省。结论 慢性乙型肝炎病毒感染者经济负担较重 。 | 陈兴宝 陈慧芬 Larry Lacey Gauri B Saal Alison Tan-Mulligan | 2002 | 肝脏2002，7，2： | 27 |
| 6 | History of the infantile hepatic hemangioma:From imaging to generating a differential diagnosis显示文摘We aim to provide an up-to-date summary of infantile hepatic hemangioma(IHH) and its misnomers and to dialectically present the differential diagnosis of these rare entities of the liver.Eligible peer-reviewed articles on hepatic infantile hemangiomas,published between 2000 and 2015,were reviewed for this study.IHH is the most common hepatic vascular tumor in children.Once a liver mass is identified in an infant,the differential diagnosis ranges from vascular malformations to benign and malignant tumors including mesenchymal hamartoma,hepatoblastoma,metastatic neuroblastoma,so careful physical examination,imaging studies,and,if indicated,tumor markers and biopsy,are of pivotal importance to ascertain the correct diagnosis.Despite the benign nature of IHHs,some of these lesions may demand medical and/or surgical intervention,especially for multiple and diffuse IHH.Complications can include hepatomegaly,hypothyroidism and cardiac failure.Therefore,a close follow-up is required until complete involution of the lesions.We propose an algorithm to guide the physicians towards the proper management of hepatic lesions. | Maria Gnarra Gerald Behr Alison Kitajewski June K Wu Sudha A Anupindi Carrie J Shawber Nick Zavras Dimitrios Schizas Chris Salakos Konstantinos P Economopoulos | 2016 | World Journal of Clinical Pediatrics2016，5，3： | 27 |
| 7 | A matched-pair analysis of laparoscopic versus open pancreaticoduodenectomy: oncological outcomes using Leeds Pathology Protocol显示文摘BACKGROUND: Laparoscopic pancreaticoduodenectomy(LPD)is a safe procedure. Oncological safety of LPD is still a matter for debate. This study aimed to compare the oncological outcomes,in terms of adequacy of resection and recurrence rate following LPD and open pancreaticoduodenectomy(OPD).METHODS: Between November 2005 and April 2009, 12LPDs(9 ampullary and 3 distal common bile duct tumors)were performed. A cohort of 12 OPDs were matched for age,gender, body mass index(BMI) and American Society of Anesthesiologists(ASA) score and tumor site.RESULTS: Mean tumor size LPD vs OPD(19.8 vs 19.2 mm,P=0.870). R0 resection was achieved in 9 LPD vs 8 OPD(P=1.000). The mean number of metastatic lymph nodes and total number resected for LPD vs OPD were 1.1 vs 2.1(P=0.140)and 20.7 vs 18.5(P=0.534) respectively. Clavien complications grade I/II(5 vs 8), III/IV(2 vs 6) and pancreatic leak(2 vs 1)were statistically not significant(LPD vs OPD). The mean high dependency unit(HDU) stay was longer in OPD(3.7 vs 1.4 days,P<0.001). There were 2 recurrences each in LPD and OPD(logrank,P=0.983). Overall mortality for LPD vs OPD was 3 vs 6(log-rank, P=0.283) and recurrence-related mortality was 2 vs 1.There was one death within 30 days in the OPD group secondary to severe sepsis and none in the LPD group.CONCLUSIONS: Compared to open procedure, LPD achieved a similar rate of R0 resection, lymph node harvest and longterm recurrence for tumors less than 2 cm. Though technically challenging, LPD is safe and does not compromise oncological outcome. | Abdul R Hakeem Caroline S Verbeke Alison Cairns Amer Aldouri Andrew M Smith Krishna V Menon | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，4： | 24 |
| 8 | 肾脏病学的新创意:“肾脏疾病:改善全球预后”(KDIGO)显示文摘慢性肾脏病(CKD)是世界范围的医疗和公共卫生问题,并开始呈现流行性的特点.美国肾脏数据系统(USRDS)的数据提示,终末期肾衰的发病率呈上升趋势,因此对于这一问题的重视日益增加.根据近期USRDS登记系统提供的资料,世界范围内需要肾脏替代治疗(RRT)的终末期肾病(ESRD)患者数目持续上升,其中我国台湾、美国和日本最多(254~365人/100万人)[1].卡塔尔和巴斯克地区的儿童患病率最高,分别为33.7人/100万人和22.7人/100万人;在大于等于20岁患者中患病率最高的是卡塔尔、美国和台湾.在欧洲,德国的患病率最高,2000年为175人/100万人.在RRT仍在发展中的东欧,报道的患病率低于西欧[2]. | Norbert Lameire Garabed Eknoyan Rashad Barsoum Kai-Uwe Eckardt Adeera Levin Nathan Levin Francesco Locatelli Alison Macleod Raymond Vanholder Rowan Walker Haiyan Wang 张路霞 王海燕 | 2005 | 中华肾脏病杂志2005，21，12： | 19 |
| 9 | 应用于三维生物打印的DNA超分子水凝胶显示文摘水凝胶因其高含水量和类似于细胞外基质的特点,是三维组织打印和人工器官制备的首选基材,也因此成为化学、材料学和生命医学领域研究的热点[1]。截止目前,用于生物打印的水凝胶主要包括海藻酸钠、壳聚糖、胶原蛋白、琼脂糖等天然水凝胶及其衍生产物,以及人工合成的聚乙二醇、聚多肽等化学类水凝胶[2]。无论是天然的物理类水凝胶还是人工合成的化学类水凝胶。 | 李闯 Alan Faulkner-Jones Alison R. Dun 金娟 陈平 邢永政 杨忠强 李志波 舒文淼 刘冬生 Rory R. Duncan | 2015 | 中国科学基金2015，29，3： | 18 |
| 10 | Enhanced recovery programme in colorectal surgery:Does one size fit all?显示文摘Enhanced recovery after surgery(ERAS) employs a multimodal perioperative care pathway with the aim of attenuating the stress response to surgery and accelerating recovery.It has been difficult to determine the relative importance of some of the individual components of these pathways such as epidural analgesia and laparoscopic colorectal surgery.Some argue that only a rigid adherence to the published ERAS protocol can achieve the proposed benefits of fast-track surgery.In this article,we explore some of the areas where the evidence base may be changing and ask whether a more flexible and individualised approach should be considered. | Alison Lyon Christopher J Payne Graham J MacKay | 2012 | World Journal of Gastroenterology2012，18，40： | 18 |
| 11 | Future Directions in Formulaic Language Research显示文摘Research tends to go in cycles of innovation,consolidation and confirmation,then reflection.Formulaic language research seems currently to be moving into a reflective phase,offering considerable potential for new ideas feeding the next round of innovation.Research into formulaic language goes back 150 years at least,with several strands developing somewhat independently,including patterns in native language (including first language acquisition),language disorders and second language learning.My own work was an attempt to identify a single explanation that accounted for them all.New technologies now provide additional ways to explore and evaluate the evidence,most notably through corpus linguistics,and eye and brain activity monitoring.Five key claims,all with potential implications for L2 research and teaching,indicate the scope for future research:1.Although we talk of an 'idiomatic' nativelike way of speaking,in fact there is considerable variation in what is idiomatic:what should be taught? 2.The acquisition of idiomaticity is driven by the need to belong and survive:can this be replicated in the classroom? 3.Although formulaic language seems to reduce the speaker' s processing,the hearer' s processing may be the more important consideration:does 'accommodation' affect the learner's experience adversely? 4.Corpus linguistics forces a careful evaluation of the true role of frequency in idiomaticity:are materials writers beguiled by corpus data? 5.We cannot consider formulaic language patterns independently of knowing the purpose of the text in which they occur,including its genre:are L2 teaching materials broad enough?There are key opportunities for researchers in China to exploit aspects of the Chinese writing system and approach to education,to answer questions that cannot be easily answered in the west.Issues include the role of memorisation in language learning,the role of literacy in our perception of linguistic units,and the universality of patterns in acquired language disorders. | Alison Wray | 2009 | 外国语2009，32，6： | 16 |
| 12 | The wonders of BMP9:From mesenchymal stem cell differentiation,angiogenesis,neurogenesis,tumorigenesis,and metabolism to regenerative medicine显示文摘Although bone morphogenetic proteins(BMPs)initially showed effective induction of ectopic bone growth in muscle,it has since been determined that these proteins,as members of the TGF-b superfamily,play a diverse and critical array of biological roles.These roles include regulating skeletal and bone formation,angiogenesis,and development and homeostasis of multiple organ systems.Disruptions of the members of the TGF-b/BMP superfamily result in severe skeletal and extra-skeletal irregularities,suggesting high therapeutic potential from understanding this family of BMP proteins.Although it was once one of the least characterized BMPs,BMP9 has revealed itself to have the highest osteogenic potential across numerous experiments both in vitro and in vivo,with recent studies suggesting that the exceptional potency of BMP9 may result from unique signaling pathways that differentiate it from other BMPs.The effectiveness of BMP9 in inducing bone formation was recently revealed in promising experiments that demonstrated efficacy in the repair of critical sized cranial defects as well as compatibility with bone-inducing bio-implants,revealing the great translational promise of BMP9.Furthermore,emerging evidence indicates that,besides its osteogenic activity,BMP9 exerts a broad range of biological functions,including stem cell differentiation,angiogenesis,neurogenesis,tumorigenesis,and metabolism.This review aims to summarize our current understanding of BMP9 across biology and the body. | Sami Mostafa Mikhail Pakvasa Elam Coalson Allen Zhu Alex Alverdy Hector Castillo Jiaming Fan Alex Li Yixiao Feng Di Wu Elliott Bishop Scott Du Mia Spezia Alissa Li Ofir Hagag Alison Deng Winny Liu Mingyang Li Sherwin S·Ho Aravind Athiviraham Michael J·Lee Jennifer Moriatis Wolf Guillermo A·Ameer Hue H·Luu Rex C·Haydon Jason Strelzow Kelly Hynes Tong-Chuan He Russell R·Reid | 2019 | Genes & Diseases2019，6，3： | 15 |
| 13 | Palmore老年知识量表的引进及验证研究显示文摘目的翻译并验证中文版Palmore老年知识量表(Facts on Aging Quiz,FAQ)。方法使用中文版FAQ调查465名注册护士的知识水平,并检验FAQ内部一致性信度、重测信度。结果 FAQ内部一致性信度为0.751,重测信度为0.982。中国护士答对的题项中位数为40.0%。结论经严格的翻译步骤,本研究得到中文版FAQ能正确体现原量表的内容,并顺应我国的文化背景,可用于评估护士的老年知识水平。 | 刘云娥 马艳林 杨文丽 Alison While 陈洁 | 2015 | 护理学报2015，22，5： | 14 |
| 14 | 供给系统、社会习俗与生活方式--中产阶层日常生活中的饮食消费变迁显示文摘中国城市中产阶层肉类消费的快速增长受到环境科学和社会政策研究的关注。本文将对北京和上海居民日常生活中肉类的供给和消费进行动态分析。数据来源为针对中产阶层的半结构式个案访谈,主要考察以下问题:(1)食品供给系统转变过程中人们食用肉类的经历;(2)在社交、庆祝活动和照顾家人时肉类食品的角色转变;(3)伴随对健康和养生的关注以及食品安全问题,饮食与保健知识逐渐交织在一起从而改变肉类消费模式。在中国中产阶层采用新形式的自我修养和对健康关注的背景下,减少吃肉正在渐渐取得合法性,但是日常实践中存在着阻碍饮食消费改变的机制。研究揭示了中国的中产阶层与肉类消费之间的动态关系,日常生活的动态--包含了以家庭、社交和健康为核心的社会文化习俗--如何同时推动和约束中国背景下的饮食习惯变迁。本研究广泛吸收社会科学和环境科学的研究文献,目的是深化对中国背景下转向可持续发展的社会实践的定性研究。 | 朱迪 Alison Browne Josephine Mylan | 2020 | 山东社会科学2020，0，3： | 11 |
| 15 | A unique sequence in the N-terminal regulatory region controls the nuclear localization of KLF8 by cooperating with the C-terminal zinc-fingers显示文摘Kr 眉 p 像像素的因素 8 (KLF8 ) 抄写因素在房间周期前进起一个关键作用, oncogenic 转变,对间充质的转变和侵略上皮。然而,它的原子本地化信号(NLS ) 没被识别。有另外的 KLF monopartite NLS (mNLS ) 和 C2H2 锌手指(ZF ) 的 KLF8 份额,哪个被显示了是为一些另外的 KLF 的 NLS。在这份报告,用指导 PCR 的 mutagenesis 和 immunofluorescent 显微镜学,我们显示出 mNLSs,任何单个 ZF 的删除,或变化的那混乱 Zn2+ 有约束力或联系 DNA 主题没影响 KLF8 的原子本地化。删除 > 然而,从 C 终点的 1.5 ZF 引起了 KLF8 的细胞质的累积。令人惊讶地,氨基酸(aa ) 的删除 151-200 区域几乎从原子核消除了 KLF8。有 PKC 禁止者的 S165A, K171E 或 K171R 变化,或处理导致了部分细胞质的累积。Co-immunoprecipitation 证明 KLF8 与 importin- 交往了,这个相互作用要求了 ZF 主题。aa 1-150 或 201-261 区域的删除独自没改变原子本地化。BrdU 加入和 cyclin D1 倡导者酶试金作为野类型的 KLF8 证明在原子本地化的 KLF8 异种有缺陷者不能支持 DNA 合成或 cyclin D1 倡导者激活。一起拿,这些结果建议 KLF8 有二 NLS,一包围 S165 和 K171 并且另外的是二双人脚踏车 ZF,它为 KLF8 原子本地化和它的细胞的功能的规定是批评的。 | Tina S Mehta Heng Lu Xianhui Wang Alison M Urvalek Kim-Hang H Nguyen Farah Monzur Jojo D Hammond Jameson Q Ma Jihe Zhao | 2009 | Cell Research2009，19，9： | 10 |
| 16 | 种源地对两种红树叶片结构和功能的影响:对温度的适应性遗传显示文摘植物叶片具有根据不同环境状况调整表型特征的能力,叶片性状对环境的适应能力直接影响植物在气候变化背景下的生存、分布和迁移。受全球气候变暖的影响,红树林正向高纬度地区扩张,然而关于红树植物叶片性状对不同生长地点温度变异的适应性遗传仍缺乏了解。该研究采用同质园法以来自6个不同纬度种群的木榄(Bruguiera gymnorhiza)和5个不同纬度种群的秋茄树(Kandelia obovata)幼苗为研究对象,测定了其叶片解剖结构和生理功能参数,分析红树幼苗叶片的解剖结构特征与生理功能之间的关系及对种源地温度的适应性遗传。结果发现:木榄幼苗的叶片厚度、栅栏组织和表皮厚度与种源地年平均气温均呈显著负相关关系,角质层厚度随年平均气温的升高呈下降的趋势;与木榄幼苗相比,秋茄树幼苗叶片具有较厚的表皮和角质层,较小的气孔密度和叶脉密度,叶片解剖结构未与种源地年平均气温表现出相关性。两种红树不同种源地幼苗的数据合在一起,叶片栅栏组织和海绵组织厚度均与光合速率呈正相关关系,与海绵组织厚度的相关性更强,说明叶肉组织厚度变化对红树光合作用有影响;叶脉密度与气孔密度、最大气孔导度也均呈显著正相关关系,说明两种红树在遗传适应上能够维持叶片水平的水分供需平衡。综上所述,两种红树叶片解剖结构存在差异,对种源地温度的适应性遗传有显著区别,木榄对长期生长环境形成适应性遗传;秋茄树通过建成性的叶片结构特征遗传适应种源地温度;叶片结构的差异引起了红树光合作用、最大气孔导度等生理功能发生相应的变化,从而有利于红树在气候变化的背景下得以生存和繁衍。 | 张小燕 WEE Kim Shan Alison KAJITA Tadashi 曹坤芳 | 2021 | 植物生态学报2021，45，11： | 10 |
| 17 | Modeling Delay and Packet Drop in Networked Control Systems Using Network Simulator NS2显示文摘Wireless Networked Control Systems (WNCS) are used to implement a control mechanism over a wireless network that is capable of carrying real-time traffic. This field has drawn enormous attention from current researchers because of its flexibility and robustness. However, designing efficient WNCS over Mobile Ad Hoc Networks (MANET) is still a challenging topic because of its less-predictable aspects, such as inconsistent delay, packet drop probability, and dynamic topology. This paper presents design guidelines for WNCS over MANET using the Network Simulator version 2, NS2 software. It investigates the impact of packet delay and packet drop under the AODV and DSR routing protocols. The simulation results have been compared to MATLAB results for validation. | Mohammad Shahidul Hasan Christopher Harding Alison Griffiths | 2005 | International Journal of Automation and computing2005，2，2： | 9 |
| 18 | 细粒棘球蚴重组抗原B的表达提取及其血清学检测初探显示文摘用基因工程技术制备出的细粒棘球绦虫重组抗原 B( r Ag B)表达载体 ,经诱导表达、亲和层析纯化获得具生物活性的重组蛋白质 r Ag B,用 Western- Blot法检测病人血清。结果显示 r Ag B敏感性为 91.6% ( 4 4/48) ,特异性为 93 .8% ( 3 0 /3 2 ) ,其中 10例泡球蚴 ( AE)病人及 10例肿瘤病人血清均无交叉反应。说明 r Ag B具有较高的敏感性及特异性 ,可用于包虫病的常规血清学诊断 ,r Ag B在宿主菌 JM10 9内稳定表达 ,因此可在实验室内大量制备用于血清学诊断的 r Ag B。 | 卢晓梅 温浩 Alison FS 付玉才 Craig PS | 2001 | 地方病通报2001，16，3： | 9 |
| 19 | 外膜孔道蛋白ompC、ompF的表达与大肠埃希菌耐药的相关性研究显示文摘目的研究外膜孔道蛋白ompC、ompF引起细胞膜通透性改变与大肠埃希菌(ECO)对抗菌药物形成耐药的相关性。方法设计复合引物对ompC全基因序列和ompF、ompA、fepA、cirA、chuA等基因序列进行扩增,回收PCR产物、钝化后测序;菌体外膜蛋白进行SDS-PAGE电泳,回收的目标膜蛋白条带经胰蛋白酶消化,采用质谱仪分析蛋白成分并进行蛋白序列的测定。结果SDS-PAGE电泳图谱和质谱分析显示,7株大肠埃希菌均有相应的外膜孔道蛋白ompC和ompA表达,无外膜孔道蛋白ompF表达;第6、7菌株的孔道蛋白ompC和ompA表达量明显减少,但有铁运输蛋白chuA、cirAf、epA的表达;基因扩增显示7株大肠埃希菌均有ompF产物,耐药性较强的第6、7菌株还有fhuA和cirA扩增产物,但产物fhuA的相对分子质量小于ECO-k12(少54 bp)。结论持续使用抗菌药物将导致细菌外膜孔蛋白ompF低表达或不表达,外膜孔蛋白ompC氨基酸序列的改变影响相应蛋白的表达,使细胞膜通透性降低,从而增加细菌对抗菌药物的耐药性;高度耐药的大肠埃希菌伴随代偿性铁运输蛋白chuA、cirA、fepA的表达,维持细菌的新陈代谢,保持其高水平耐药性。 | 戎建荣 王淑峰 Alison S.Low Ian R.Booth | 2009 | 中华医院感染学杂志2009，19，6： | 9 |
| 20 | 中国e抗原阳性慢性乙型肝炎抗病毒治疗方案的经济学评价显示文摘目的 对HBeAg阳性慢性乙型肝炎短期和长期的抗病毒治疗方案进行经济学评价。方法 建立包含10个健康状态的Markov模型,估计不同方案治疗HBeAg阳性CHB患者的长期效果和费用,并进行增量分析。结果 与非抗病毒治疗相比,拉米夫定治疗1年是具有高度成本效果的短期治疗方案;拉米夫定治疗必要时换用阿德福韦,或者阿德福韦治疗必要时换用拉米夫定作挽救用药治疗5年,可以更持续地延缓疾病进展。与拉米夫定治疗1年相比,更具成本效果的长期治疗方案则是拉米夫定+阿德福韦,或者阿德福韦+拉米夫定作为挽救用药治疗5年,每单位质量调整生命年的增量费用分别为25 115元和35 577元,分别比国际阈值的中国估计值低55.2%和36.5%。结论 在HBeAg阳性CHB短期治疗方案中,拉米夫定比IFNα、恩替卡韦、聚乙二醇干扰素α-2a和非抗病毒治疗更具成本效果。在长期治疗方案中,拉米夫定+阿德福韦或阿德福韦+拉米夫定挽救治疗均具有成本效果。 | Larry Lacey 卢宪中 Alison Tan | 2007 | 中华肝脏病杂志2007，15，6： | 8 |
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