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| 1 | Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post-harvest Citrus Fruit显示文摘Citric acid plays an important role in fresh fruit favor and its adaptability to post-harvest storage conditions.In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit,systematic biological analyses were conducted on stored Hirado Buntan Pummelo(HBP;Citrus grandis)fruits.High-performance capillary electrophoresis,subcellular organelle expression microarray,real-time quantitative reverse transcription polymerase chain reaction,gas chromatography mass spectrometry(GC-MS),and conventional physiological and biochemical analyses were undertaken.The results showed that the concentration of organic acids in HBP underwent a regular fuctuation.GC-MS-based metabolic profling indicated that succinic acid,g-aminobutyric acid(GABA),and glutamine contents increased,but 2-oxoglutaric acid content declined,which further confrmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes.In addition,the concentration of organic acids was signifcantly correlated with senescence-related physiological processes,such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities,which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes. | Xiaohua Sun Andan Zhu Shuzhen Liu Ling Sheng Qiaoli Ma Li Zhang Elsayed Mohamed Elsayed Nishawy Yunliu Zeng Juan Xu Zhaocheng Ma Yunjiang Cheng Xiuxin Deng | 2013 | Journal of Integrative Plant Biology2013，55，11： | 14 |
| 2 | Integrated transcriptomic and metabolomic analyses of a wax deficient citrus mutant exhibiting jasmonic acid-mediated defense against fungal pathogens显示文摘Naturally,resistant crop germplasms are important resources for managing the issues of agricultural product safety and environment deterioration.We found a spontaneous mutant of‘Newhall’navel orange(Citrus sinensis Osbeck)(MT)with broad-spectrum protections against fungal pathogens in the orchard,postharvest-storage,and artificial inoculation conditions.To understand the defense mechanism of MT fruit,we constructed a genome-scale metabolic network that integrated metabolome and transcriptome datasets.The coordinated transcriptomic and metabolic data were enriched in two sub-networks,showing the decrease in very long chain fatty acid(by 41.53%)and cuticular wax synthesis(by 81.34%),and increase in the synthesis of jasmonic acid(JA)(by 95.23%)and JA-induced metabolites such as 5-dimethylnobietin(by 28.37%)in MT.Furthermore,cytological and biochemical analyses confirmed that the response to fungal infection in MT was independent of wax deficiency and was correlated with the levels of jasmonates,and the expression of plant defensin gene PDF1.2.Results of exogenous application of MeJA and JA inhibitors such as propyl gallate proved that JA-mediated defense contributes to the strong tolerance against pathogens in MT.Our results indicated that jasmonate biosynthesis and signaling are stimulated by the fatty acid redirection of MT,and participate in the tolerance of pathogenic fungi. | Yizhong He Jingwen Han Runsheng Liu Yuduan Ding Jinqiu Wang Li Sun Xiaoming Yang Yunliu Zeng Weiwei Wen Juan Xu Hongming Zhang Xiang Yan Zhaoxing Chen Zuliang Gu Hong Chen Huanqing Tang Xiuxin Deng Yunjiang Cheng | 2018 | Horticulture Research2018，5，1： | 8 |
| 3 | Investigation on fracture models and ground pressure distribution of thick hard rock strata including weak interlayer显示文摘Dynamic disasters,such as rock burst due to the breaking of large area stiff roof strata,are known to occur in the hard rock strata of coal mines.In this paper,mechanical models of the fracturing processes of thick hard rock strata were established based on the thick plate theory and numerical simulations.The results demonstrated that,based on the fracture characteristics of the thick hard rock strata,four fracture models could be analyzed in detail,and the corresponding theoretical failure criteria were determined in detail.In addition,the influence of weak interlayer position on the fracture models and ground pressure of rock strata is deeply analyzed,and six numerical simulation schemes have been implemented.The results showed that the working face pressure caused by the independent movement of the lower layer is relatively low.The different fracture type of the thick hard rock strata had different demands on the working resistance of the hydraulic powered supports.The working resistance of the hydraulic powered supports required by the stratified movements was lower than that of the non-stratified movements. | Meilu Yu Jianping Zuo Yunjiang Sun Changning Mi Zhengdai Li | 2022 | International Journal of Mining Science and Technology2022，32，1： | 5 |
| 4 | Microarray Expression Profiling of Postharvest Ponkan Mandarin (Citrus reticulata) Fruit under Cold Storage Reveals Regulatory Gene Candidates and Implications on Soluble Sugars Metabolism显示文摘Low temperature storage is widely applied to maintain citrus postharvest fruit quality.In this study,the transcriptional and metabolic changes in the pulp tissue of Citrus reticulata Blanco cv.'Ponkan' were studied for three successive months under cold storage by Affymetrix Citrus GeneChip and gas chromatography,respectively.As many as 2 161 differentially expressed transcripts were identified based on the bayesian hierarchical model.The statistical analysis of gene ontology revealed that defense/stress-related genes were induced quickly,while autophagy-related genes were overrepresented in the late sampling stages,suggesting that the functional shift may coincide with the subsequent steps of chilling development.We further classified the potential regulatory components and concluded that ethylene may play the crucial role in chilling development in this non-climacteric fruit.To cope with complex events,53 upregulated transcription factors represented regulatory candidates.Within these,the AP2-EREBP,C2H2 and AS2 gene family were overrepresented.Cold storage also causes alterations in various metabolic pathways;a keen interest is paid in deciphering expression changes of soluble sugar genes as increased evidence that soluble sugars act as both osmolytes and metabolite signal molecules.Our results will likely facilitate further studies in this field with promising genetic candidates during chilling. | Andan Zhu Wenyun Li Junli Ye Xiaohua Sun Yuduan Ding Yunjiang Cheng Xiuxin Deng | 2011 | Journal of Integrative Plant Biology2011，53，5： | 3 |
| 5 | Origin area and migration route: Chloroplast DNA diversity in the arctic-alpine plant Koenigia islandica显示文摘The Hengduan Mountains(henceforth H-D Mountains) on the Tibet Plateau are a distribution and diversity center for many alpine genera. We examine patterns of genetic variation in an arctic-alpine plant to evaluate the possibility that the H-D Mountains constitute the area of origin of the species as well as to uncover postglacial migration routes. 220 individuals of the arctic-alpine plant Koenigia islandica were sampled from 26 populations distributed in western China and northern Finland. DNA haplotypes were identified using restriction site analysis of two chloroplast DNA intergene spacer regions, atpB-rbcL and trnL-trnF. We examined the geographical distribution of haplotype diversity in relation to latitude, and also compared various indices of diversity in putatively glaciated and unglaciated regions. Patterns of migration were inferred using nested clade analysis. A total of 25 haplotypes were detected. High haplotype diversity was found in the H-D Mountains. H3 and its radiated haplotypes were distributed in the Himalayas. Two haplotypes were fixed concurrently in the H-D Mountains and northern Finland. High genetic diversity of K. islandica and high species diversity of K. islandica are expected in the origin area. Our observations suggest that the H-D Mountains are not only the place of origin of K. islandica, but also the refugia for K. islandica on the Tibet Plateau. What is more, the migration route for the arctic-alpine plant K. islandica must have originated in the region defined by the H-D Mountains in western China extending northward to the Arctic circumpolar, and moved westward along the Himalayas, then northward across the Altay Mountains and the Central Siberian Plateau at different time periods. | LONG Cong MIN YunJiang ZHAO XiuXia YANY ChunLei SUN Hang Lü HouYuan TANG LingYu ZHOU ZhongZe | 2014 | Science China Earth Sciences2014，57，8： | 2 |
| 6 | Fiber-Optic Microstructure Sensors:A Review显示文摘This paper reviews a wide variety of fiber-optic microstructure(FOM)sensors,such as fiber Bragg grating(FBG)sensors,long-period fiber grating(LPFG)sensors,Fabry-Perot interferometer(FPI)sensors,Mach-Zchnder interferometer(MZI)sensors,Michelson interferometer(MI)sensors,and Sagnac interferometer(SI)sensors.Each FOM sensor has been introduced in the terms of structure types,fabrication methods,and their sensing applications.In addition,the sensing characteristics of different structures under the same type of FOM sensor are compared,and the sensing characteristics of the all FOM sensors,including advantages,disadvantages,and main sensing parameters,are summarized.We also discuss the future development of FOM sensors. | Zengling RAN Xiu HE Yunjiang RAO Dong SUN Xiaojuan QIN Debiao ZENG Wangwei CHU Xiankun LI Yabin WEI | 2021 | Photonic Sensors2021，11，2： | 1 |
| 7 | Clinical evaluation of the first domestically produced generic isosulfan blue injection in sentinel lymph node biopsy for early breast cancer in China:a multicenter,single-arm,open validation trial(CSBrS-024)显示文摘In 2020,the first generic isosulfan blue product for injection(notification number:2020 LP00901,batch number:118210301,5 mL:50 mg,Guangdong Hebo Pharmaceutical Co.,Ltd.)was approved by the National Medical Products Administration of China.This multicenter,single-arm,phase III,open clinical validation trial was organized by the Chinese Society of Breast Surgery to evaluate the efficacy and safety of this domestically produced generic isosulfan blue injection for sentinel lymph node biopsy(SLNB)in patients with early breast cancer(BC)in China. | Fengjiang Qu Di Wu Yunjiang Liu Wei Gao Shu Wang Siyuan Wang Feng Jin Bo Chen Jian Huang Shanshan Sun Zhimin Fan Chinese Society of Breast Surgery | 2022 | Chinese Medical Journal2022，，14： | 0 |
| 8 | Predicting metal-organic frameworks as catalysts to fix carbon dioxide to cyclic carbonate by machine learning显示文摘The process of discovering and developing new materials currently requires considerable effort,time,and expense.Machine learning(ML)algorithms can potentially provide quick and accurate methods for screening new materials.In the present work,the features of the metal organic frameworks(MOFs)as a catalyst for fixing carbon dioxide into cyclic carbonate were extracted to build a data set,which were collected from the experimental results of approximately 100 published papers.Classifiers were trained with the data set with various ML algorithms,including support vector machine(SVM),K-nearest neighbor classification(KNN),decision trees(DT),stochastic gradient descent(SGD),and neural networks(NN),to predict the catalytic performance.The ML models were trained on 80% of the data set and then tested on the remaining 20%to predict the carbon dioxide fixation ability.The trained ML model was extended to explore 1311 hypothetical MOFs,and some structures displayed a strong catalytic ability.Finally,the six best metal ions(Mn,V,Cu,Ni,Zr and Y)and four best ligands(tactmb,tdcbpp,TCPP,H_(3)L)were determined.These six metals and four ligands could be combined into 24 MOFs,which are strongly potential catalysts for carbon dioxide fixation.Using machine learning methods can speed up the screening of materials,and this methodology is promising for application not only to MOFs as catalysts but also in many other materials science projects. | Shuyuan Li Yunjiang Zhang Yuxuan Hu Bijin Wang Shaorui Sun Xinwu Yang Hong He | 2021 | Journal of Materiomics2021，7，5： | 0 |
| 9 | Transcription factor CsTT8 promotes fruit coloration by positively regulating the methylerythritol 4-phosphate pathway and carotenoid biosynthesis pathway in citrus (Citrus spp.)显示文摘Carotenoids directly influence citrus fruit color and nutritional value,which is critical to consumer acceptance.Elucidating the potential molecular mechanism underlying carotenoid metabolism is of great importance for improving fruit quality.Despite the well-established carotenoid biosynthetic pathways,the molecular regulatory mechanism underlying carotenoid metabolism remains poorly understood.Our previous studies have reported that the Myc-type basic helix-loop-helix(bHLH)transcription factor(TF)regulates citrus proanthocyanidin biosynthesis.Transgenic analyses further showed that overexpression of CsTT8 could significantly promote carotenoid accumulation in transgenic citrus calli,but its regulatory mechanism is still unclear.In the present study,we found that overexpression of CsTT8 enhances carotenoid content in citrus fruit and calli by increasing the expression of CsDXR,CsHDS,CsHDR,CsPDS,CsLCYE,CsZEP,and CsNCED2,which was accompanied by changes in the contents of abscisic acid and gibberellin.The in vitro and in vivo assays indicated that CsTT8 directly bound to the promoters of CsDXR,CsHDS,and CsHDR,the keymetabolic enzymes of the methylerythritol 4-phosphate(MEP)pathway,thus providing precursors for carotenoid biosynthesis and transcriptionally activating the expression of these three genes.In addition,CsTT8 activated the promoters of four key carotenoid biosynthesis pathway genes,CsPDS,CsLCYE,CsZEP,and CsNCED2,directly promoting carotenoid biosynthesis.This study reveals a novel network of carotenoid metabolism regulated by CsTT8.Our findings will contribute to manipulating carotenoid metabolic engineering to improve the quality of citrus fruit and other crops. | Quan Sun Zhengchen He Ranran Wei Yingzi Yin Junli Ye Lijun Chai Zongzhou Xie Wenwu Guo Juan Xu Yunjiang Cheng Qiang Xu Xiuxin Deng | 2023 | Horticulture Research2023，10，11： | 0 |
| 10 | NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP, NUCLEAR SSR AND WHOLE-GENOME SEQUENCING显示文摘Kumquat(Fortunella spp.)is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its fruit.Although Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus,few studies have been focused on its evolutionary scenario.In this study,analysis of five chloroplast loci and 47 nuclear microsatellites(nSSR)loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa,and that Fortunella mainly comprises two populations:CUL,cultivated Fortunella spp.(F.margarita,F.crassifolia and F.japonica);and HK,wild Hong Kong kumquat(Fortunella hindsii).Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model,suggesting directional selection was a force driving their evolutions.CUL exhibited lower genomic diversity and higher linkage strength than HK,suggesting artificial selection involved in its origin.A high level of genetic differentiation(Fst=0.364)was detected and obviously asynchronous demographic changes were observed between CUL and HK.Based on these results,a new hypothesis for the speciation of Fortunella is proposed. | Chenqiao ZHU Peng CHEN Junli YE Hang LI Yue HUANG Xiaoming YANG Chuanwu CHEN Chenglei ZHANG Yuantao XU Xiaoli WANG Xiang YAN Guangzhou DENG Xiaolin JIANG Nan WANG Hongxing WANG Quan SUN Yun LIU Di FENG Min YU Xietian SONG Zongzhou XIE Yunliu ZENG Lijun CHAI Qiang XU Chongling DENG Yunjiang CHENG Xiuxin DENG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，4： | 0 |
| 11 | The underappreciated role of fugitive VOCs in ozone formation and health risk assessment emitted from seven typical industries in China显示文摘Fugitive emission from industrial sources may result in ozone formation and health risk,while the exact contribution of this source remains incompletely understood.In this study,emission characteristics,ozone formation potential(OFP)and health risk of fugitive VOCs in7 representative industries were investigated.Chemical material industry was the dominant contributor to VOCs of fugitive emission in comparison with other industries.The OFP of VOCs from fugitive emission was in the range of 1.45×10^(3)-3.98×10^(5)μg/m^(3),with a higher value than that of organized emission in seven industries except for the coking industry and the chemical material industry,suggesting that fugitive VOCs should be taken into account while developing control strategies.Acetaldehyde,m,p-xylene,n-nonane,ethylene,vinyl chloridethe and other high OFP-contributing species were the major reactive species that should be targeted.Health risk assessment investigated non-cancer and cancer risks of fugitive VOCs in 7 industries were all above safe level(HR>1 and LCR>1×10^(-4)),posing remarkable health threats to human health.OVOCs were the main contributor to non-cancer risk,while halohydrocarbons and aromatics contributed most to cancer risks,posing remarkable health threat on human health.Our findings highlighted the contribution of fugitive VOCs on ozone formation and health risk was underestimated,indicating which should be considered in emission control strategies of industrial sources. | Zhiling Liang Yunjiang Yu Bingbing Sun Qian Yao Xihua Lin Yongsheng Wang Jianping Zhang Yingzi Li Xuefeng Wang Zhengzheng Tang Shexia Ma | 2024 | Journal of Environmental Sciences2024，，2： | 0 |
| 12 | CACA Guidelines for Holistic Integrative Management of Breast Cancer显示文摘Purpose:Breast cancer is now the most common malignant tumor worldwide.About one-fourth of female cancer patients all over the world sufer from breast cancer.And about one in six female cancer deaths worldwide is caused by breast cancer.In terms of absolute numbers of cases and deaths,China ranks frst in the world.The CACA Guidelines for Holistic Integrative Management of Breast Cancer were edited to help improve the diagnosis and comprehensive treatment in China.Methods:The Grading of Recommendations Assessment,Development and Evaluation(GRADE)was used to classify evidence and consensus.Results:The CACA Guidelines for Holistic Integrative Management of Breast Cancer include the epidemiology of breast cancer,breast cancer screening,breast cancer diagnosis,early breast cancer treatment,advanced breast cancer treatment,follow-up,rehabilitation,and traditional Chinese medicine treatment of breast cancer patients.Conclusion:We to standardize the diagnosis and treatment of breast cancer in China through the formulation of the CACA Guidelines. | Jiong Wu Daiming Fan Zhimin Shao Binghe Xu Guosheng Ren Zefei Jiang Yongsheng Wang Feng Jin Jin Zhang Qingyuan Zhang Fei Ma Jinli Ma Zhonghua Wang Shusen Wang Xiaojia Wang Shu Wang Haibo Wang Tao Wang Xiang Wang Jing Wang Jia Wang Biyun Wang Li Fu Hongyuan Li Yehui Shi Lu Gan Yunjiang Liu Jian Liu Zhenzhen Liu Qiang Liu Qiang Sun Wenwu Cheng Keda Yu Zhongsheng Tong Xinhong Wu Chuangui Song Jianguo Zhang Jian Zhang Junjie Li Bin Li Man Li Huiping Li Wentao Yang Hongjian Yang Benlong Yang Hong Bu Juping Shen Zhenzhou Shen Yiding Chen Ceshi Chen Da Pang Zhimin Fan Ying Zheng Xiaoli Yu Guangyu Liu Xichun Hu Yiqun Ling Jinhai Tang Yongmei Yin Cuizhi Geng Peng Yuan Yajia Gu Cai Chang Xuchen Cao Yuan Sheng Yuanxi Huang Jian Huang Weijun Peng Xiaohua Zeng Yuntao Xie Ning Liao Committee of Breast Cancer Society,Chinese Anti-Cancer Association | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
| 13 | Modeling methane dynamics in three wetlands in Northeastern China by using the CLM-Microbe model显示文摘Wetlands account for up to 70%of the natural source of methane(CH_(4))in terrestrial ecosystems on a global scale.Soil microbes are the ultimate producers and biological consumers of CH_(4)in wetlands.Therefore,simulating microbial mechanisms of CH_(4)production and consumptionwould improve the predictability of CH_(4)flux in wetland ecosystems.In this study,we applied a microbial-explicit model,the CLM-Microbe,to simulate CH_(4)flux in three major natural wetlands in northeastern China.The CLM-Microbe model was able to capture the seasonal variation of gross primary productivity(GPP),dissolved organic carbon(DOC),and CH_(4)flux.The CLM-Microbe model explained more than 40%of the variation in GPP and CH_(4)flux across sites.Marsh wetlands had higher CH_(4)flux than mountain peatlands.Ebullition dominated the CH_(4)transport pathway in all three wetlands.The methanogenesis dominates while methanotroph makes a minor contribution to the CH_(4)flux,making all wetlands a CH_(4)source.Sensitivity analysis indicated that microbial growth and death rates are the key factors governing CH_(4)emission and vegetation physiological properties(flnr)and maintenance respiration predominate GPP variation.Explicitly simulating microbial processes allows genomic information to be incorporated,laying a foundation for better predicting CH_(4)dynamics under the changing environment. | Yunjiang Zuo Yihui Wang Liyuan He Nannan Wang Jianzhao Liu Fenghui Yuan Kexin Li Ziyu Guo Ying Sun Xinhao Zhu Lihua Zhang Changchun Song Li Sun Xiaofeng Xu | 2022 | Ecosystem Health and Sustainability2022，8，1： | 0 |
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