|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 2 | Reduction of tumorigenicity of SMMC-7721 hepatoma cells by vascular endothelial growth factor antisense gene therapy显示文摘AIM To test the hypothesis to block VEGFexpression of SMMC-7721 hepatoma cells mayinhibit tumor growth using the rat hepatomamodel.METHODS Amplifiy the 200 VEGF cDNAfragment and insert it into human U6 genecassette in the reverse orientation transcribingsmall antisense RNA which could specificallyinteract with VEGF165, and VEGF121 mRNA.Construct the retroviral vector containing thisantisense VEGF U6 cassette and package thereplication-deficient recombinant retrovirus.SMMC-7721 cells were transduced with thesevirus and positive clones were selected withG418. PCR and Southern blot analysis wereperformed to determine if U6 cassette integratedinto the genomic DNA of positive clone.Transfected tumor cells were evaluated for RNAexpression by ribonuclease protection assays.The VEGF protein in the supernatant of parentaltumor cells and genetically modified tumor cellswas determined with ELISA. In vitro and in vivogrowth properties of antisense VEGF cell clonein nude mice were analyzed.RESULTS Restriction enzyme digestion andPCR sequencing verified that the antisense VEGFRNA retroviral vector was successfullyconstructed. After G418 selection, resistantSMMC-7721 cell clone was picked up. PCR andSouthern blot analysis suggested that U6cassette was integrated into the cell genomicDNA. Stable SMMC-7721 cell clone transducedwith U6 antisense RNA cassette could express200bp small antisense VEGF RNA and secretereduced levels of VEGF in culture condition.Production of VEGF by antisense transgeneexpressing cells was 65 ± 10 ng / L per 106 cells,420 ± 45 ng/L per 106 cells in sense group and 485± 30 ng/L per 106 cells in the negative control group, (P<0.05). The antisense-VEGF cell clone appeared phenotypically indistinguishable from SMMC-7721 cells and SMMC-7721 cells transfected sense VEGF. The growth rate of the antisense-VEGF cell clone was the same as the control cells. When S. C. was implanted into nude mice, growth of antisense-VEGF cell lines was greatly inhibited compared with control cells.CONCLUSION Expression of antisense VEGFRNA in SMMC-7721 cells could decrease thetumorigenicity, and antisense-VEGF genetherapy may be an adjuvant treatment forhepatoma. | Yu Cheng Tang Yu Li Guan Xiang Qian Department of Biochemistry, Shanghai Second Medical University, Shanghai 200025, China | 2001 | World Journal of Gastroenterology2001，7，1： | 33 |
| 3 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 4 | The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism显示文摘在动物农业和药的抗菌素的 overuse 引起了一系列潜在的威胁到公共健康。Macleaya cordata 是从 Papaveraceae 家庭的药用的植物种,提供为抗菌剂的制造的一个安全资源为家畜喂添加剂。从 M. 的活跃成分 ? cordata 被知道包括 benzylisoquinoline 碱(偏爱) 象 sanguinarine (圣) 和 chelerythrine (CHE ) 那样的 ?,而是他们的新陈代谢的小径还得在这非模型植物被学习。在 M. 的圣和 CHE 的活跃生合成 ? cordata 被喂 13标记 C 的酷氨酸。为了增加,推进卓见,我们 de novo 定序 M 的整个染色体。cordata,第一从 Papaveraceae 家庭被定序。M.? 盖住 378 Mb 的 cordata 染色体与 43.5%being 编码 22,328 预言的编码蛋白质的基因 transposable 元素。作为基础 eudicot 的一个成员, M.? cordata 染色体缺乏 ? 发生在几乎所有 eudicots 的 paleohexaploidy 事件。从 genomics 数据, 16 的一个完全的集合 ? 新陈代谢 ? 为圣和 CHE 生合成的基因被检索,并且 14 项他们的生物化学的活动被验证。这些 genomics 和新陈代谢的数据在 M. 显示出保存 BIA 新陈代谢的小径 ? cordata 并且由庄稼改进或微生物引起的小径重建为圣和 CHE 的未来生产提供知识基础。 | Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng | 2017 | Molecular Plant2017，10，7： | 19 |
| 5 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
| 6 | A long-term Global LAnd Surface Satellite(GLASS)data-set for environmental studies显示文摘Recently,five Global LAnd Surface Satellite(GLASS)products have been released:leaf area index(LAI),shortwave broadband albedo,longwave broadband emissivity,incident short radiation,and photosynthetically active radiation(PAR).The first three products cover the years 19822012(LAI)and 19812010(albedo and emissivity)at 15 km and 8-day resolutions,and the last two radiation products span the period 20082010 at 5 km and 3-h resolutions.These products have been evaluated and validated,and the preliminary results indicate that they are of higher quality and accuracy than the existing products.In particular,the first three products have much longer time series,and are therefore highly suitable for various environmental studies.This paper outlines the algorithms,product characteristics,preliminary validation results,potential applications and some examples of initial analysis of these products. | Shunlin Liang Xiang Zhao Suhong Liu Wenping Yuan Xiao Cheng Zhiqiang Xiao Xiaotong Zhang Qiang Liu Jie Cheng Hairong Tang Yonghua Qu Yancheng Bo Ying Qu Huazhong Ren Kai Yu John Townshend | 2013 | International Journal of Digital Earth2013，6，S01： | 16 |
| 7 | Evolution of boundary layer ozone in Shijiazhuang,a suburban site on the North China Plain显示文摘The structure of the boundary layer affects the evolution of ozone(O3), and research into this structure will provide important insights for understanding photochemical pollution.In this study, we conducted a one-month observation(from June 15 to July 14, 2016) of the boundary layer meteorological factors as well as O3 and its precursors in Luancheng County,Shijiazhuang(37°53′N, 114°38′E). Our research showed that photochemical pollution in Shijiazhuang is serious, and the mean hourly maximum and mean 8-hr maximum O3 concentrations are 97.9 ± 26.1 and 84.4 ± 22.4 ppbV, respectively. Meteorological factors play a significant role in the formation of O3. High temperatures and southeasterly winds lead to elevated O3 values, and at moderate relative humidity(40%–50%) and medium boundary layer heights(1200–1500 m), O3 production sensitivity occurred in the transitional region between volatile organic compounds(VOC) and nitrogen oxides(NOx) limitations,and the O3 concentration was the highest. The vertical profiles of O3 were also measured by a tethered balloon. The results showed that a large amount of O3 was stored in the residual layer, and the concentration was positively correlated with the O3 concentration measured the previous day. During the daytime of the following day, the contribution of O3 stored in the residual layer to the boundary layer reached 27%± 7% on average. | Wei Zhao Guiqian Tang Huan Yu Yang Yang Yinghong Wang Lili Wang Junlin An Wenkang Gao Bo Hu Mengtian Cheng Xingqin An Xin Li Yuesi Wang | 2019 | Journal of Environmental Sciences2019，31，9： | 15 |
| 8 | New prospecting progress using information and big data of coal and oil exploration holes on sandstone-type uranium deposit in North China显示文摘1.Objective A series of Mesozoic-Cenozoic continental sedimentary basins exist in North China,coexisting with coal,oil/gas,and salt resources.Many previous drilling projects have been conducted within these basins to explore coal,petroleum,and mineral resources,however,these data have not been integrated due to different industries owners.In order to efficiently explore the large-sized,easily extracted,and environmentally friendly,sandstone-type uranium deposits,previous coal and oil exploration drilling-hole data are systematically collected, processed,and analyzed to improve the sandstone-type uranium prospecting exploitation.At the same time,we also discussed the uranium source,ore-forming process and model for the sandstone-type uranium deposits. | Ruo-shi Jin Pei-sen Miao Xian-zhang Sima Reng-An Yu Yin-hang Cheng Chao Tang Tian-fu Zhang Cong Ao Xue-ming Teng | 2018 | China Geology2018，1，1： | 12 |
| 9 | Mapping the permafrost stability on the Tibetan Plateau for 2005–2015显示文摘Data scarcity is a major obstacle for high-resolution mapping of permafrost on the Tibetan Plateau(TP).This study produces a new permafrost stability distribution map for the 2010 s(2005–2015)derived from the predicted mean annual ground temperature(MAGT)at a depth of zero annual amplitude(10–25 m)by integrating remotely sensed freezing degree-days and thawing degree-days,snow cover days,leaf area index,soil bulk density,high-accuracy soil moisture data,and in situ MAGT measurements from 237 boreholes on the TP by using an ensemble learning method that employs a support vector regression model based on distance-blocked resampled training data with 200 repetitions.Validation of the new permafrost map indicates that it is probably the most accurate of all currently available maps.This map shows that the total area of permafrost on the TP,excluding glaciers and lakes,is approximately 115.02(105.47–129.59)×10^4 km^2.The areas corresponding to the very stable,stable,semi-stable,transitional,and unstable types are 0.86×10^4,9.62×10^4,38.45×10^4,42.29×10^4,and 23.80×10^4 km^2,respectively.This new map is of fundamental importance for engineering planning and design,ecosystem management,and evaluation of the permafrost change in the future on the TP as a baseline. | Youhua RAN Xin LI Guodong CHENG Zhuotong NAN Jinxing CHE Yu SHENG Qingbai WU Huijun JIN Dongliang LUO Zhiguang TANG Xiaobo WU | 2021 | Science China Earth Sciences2021，64，1： | 11 |
| 10 | Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery显示文摘寒心的损害的症状是在射击的水赤字的开发,源于水运输和蒸发的不平衡。在这个工作,二个米饭变化(Oryza sativa L。var。Wasetoitsu 和 Somewake ) 幼苗是在 7 度 C 的 chilled,在 28 度 C 由恢复列在后面。基于生长显型和电解质漏测试, Somewake 被显示是一个寒心容忍的变化,和 Wasetoitsu 寒心敏感的。寒心的压力显著地减少了叶子,积聚的蒸发和渗透的根的相对的水内容(RWC ) 在两个变化的水力的电导率(Lp ) 。但是当回到了 28 度 C 时, Somewake 的水关系平衡更好恢复了。所有 11 血浆膜的 mRNA 表示侧面内在的蛋白质(果仁) , aquaporins 的亚群,被有 TaqMan 次要的林子文件夹(MGB ) 的 -PCR 探查的即时反向的抄写(RT ) 随后决定源于米饭 var。Nipponbare 在寒心的处理和恢复期间。大多数 PIP 基因在低温度是下面调整的,并且在温暖的温度恢复了。在 Somewake 和 Wasetoitsu 的一些果仁的相对表示在平行减少了在期间寒心。然而在恢复期间, OsPIP1 的相对表示; 1, OsPIP2; 1, OsPIP2; 7 在射击和 OsPIP1; 1, OsPIP2; 1 比 Wasetoitsu 在 Somewake 在根是显著地更高的。这在在寒心的条件以后重建水平衡支持果仁的角色。我们讨论 aquaporin 果仁亚科的成员在植物起的多样化的作用寒心的忍耐取决于 aquaporin isoforms,植物织物和寒心的持续时间的阶段。 | Xin Yu Yan Hui Peng Min Hua Zhang Yan Jun Shao Wei Ai Su Zhang Cheng Tang | 2006 | Cell Research2006，16，6： | 11 |
| 11 | Rice OsGL 1-1 Is Involved in Leaf Cuticular Wax and Cuticle Membrane显示文摘表皮的蜡在天线植物机关上形成一个恐水病的障碍;它在保护植物免受环境应力的许多形式引起的损坏的伤害起一个重要作用。在现在的学习,我们描绘了蜡缺乏的变异的 osgl1-1 从一个自发的变化导出的一片米饭叶,它展出了蜡缺乏、高度吸水的叶显型。我们由基于地图的克隆方法克隆 OsGL1-1 基因并且执行了互补测试证实候选人基因的功能。分子的研究表明 OsGL1-1 是 OsGL1 家庭的一个成员,并且包含了对在甾醇 desaturases 和短链的 dehydrogenases/reductases 的一些区域相应的区域。比作野类型, osgl1-1 异种显示出减少的表皮的蜡免职,更薄的表皮的膜,沥滤的减少的叶绿素,水损失的增加的率,和提高的敏感到干旱。OsGL1-1 在米饭无所不在地被表示。OsGL1-1green 的短暂表示荧光灯蛋白质熔化蛋白质显示 OsGL1-1 在细胞质,血浆膜,和原子核是局部性的。 | Bao-Xiang Qin Ding Tang Jian Huang Ming Li Xin-Ru Wu Li-Li LU Ke-Jian Wang Heng-Xiu Yu Jian-Min Chen Ming-Hong Gu Zhu-Kuan Cheng | 2011 | Molecular Plant2011，4，6： | 10 |
| 12 | Incidence and risk factors of tuberculosis among the elderly population in China:a prospective cohort study显示文摘Background:China is facing challenges of the shifting presentation of tuberculosis(TB)from younger to elderly due to an ageing population,longer life expectancy and reactivation disease.However,the burden of elderly TB and influence factors are not yet clear.To fill the gap,we generated a cohort study to measure the magnitude of TB incidence and associated factors among the elderly population aged 65 years and above in China.Methods:In this cohort established in 2013 through a prevalence survey conducted in selected sites,a total of 34076 elderlies without TB were enrolled into two-year follow-up.We used both active and passive case findings to find out all TB patients among them.The person-year(PY)incidence rates for both bacteriologically positive TB and active TB were calculated.Cox proportional regression model was performed to test effect of risk factors,and the population attributable fraction(PAF)of each risk factor contributing to incident TB among elderlies was calculated.Results:Over the two-year follow-up period,a total of 215 incident active TB were identified,62 of which were bacteriologically positive.The incidence rates for active TB and bacteriologically positive TB were 481.8 per 100000 PY(95%CI:417.4–546.2 per 100000 PY)and 138.9 per 100000 PY(95%CI:104.4–173.5 per 100000 PY),respectively.Incident cases detected by active case finding were significantly higher(P<0.001).Male,non-Han nationality,previously treated TB,ex/current smoker and body mass index(BMI)<18.5 presented as independent predictors for developing TB disease.For developing bacteriologically positive TB,the biggest contribution was from self-reported ex or current smoker(18.06%).And,for developing active TB,the biggest contribution was from non-Han nationality(35.40%),followed by male(26.80%)and age at 75 years and above(10.85%).Conclusions:Ageing population in China had a high TB incidence rate and risk to develop TB disease,implying that National TB Program(NTP)needs to prioritize for elderly.Active case finding should be applied capture more active TB cases among this particular population,especially for male,non-Han nationality,and those with identified risk factors. | Jun Cheng Yan-Ni Sun Can-You Zhang Yan-Ling Yu Li-Hong Tang Hong Peng Ying Peng Yu-Xia Yao Shuang-Yi Hou Jian-Wei Li Jin-Ming Zhao Lan Xia Lin Xu Yin-Yin Xia Fei Zhao Li-Xia Wang Hui Zhang | 2020 | Infectious Diseases of Poverty2020，9，1： | 10 |
| 13 | Cognitive impairment and the associated risk factors among the elderly in the Shanghai urban area:a pilot study from China显示文摘Objectives:Our study aimed to investigate the prevalence of cognitive impairment(CI)and the associated risk factors among elderly people in Shanghai urban area,China.Methods:A population-based survey was conducted among people aged 55 years or older in urban areas of Shanghai.Face-to-face interviews were carried out to collect information including demographic characteristics,medical history,and medication use,etc.The validated Chinese version of the Mini-Mental State Examination(MMSE)was used to screen subjects with CI,and the criteria of CI were adjusted for education levels.Results:A total of 3,176 home-living residents(≥55 years old)were included in the study.Among them,266 people(102 men and 164 women)were identified as cognition impaired,with a prevalence of 8.38%(266/3,176,95%CI:(8.26,8.49))for both genders,9.21%(102/1,107,95%CI:(9.18,9.33))for men and 7.93%(164/2,069,95%CI:(7.80,8.09))for women,respectively.Furthermore,we found that several significant risk factors,including social factors(education,number of children,marriage status,and family structure),physiological factors(age,blood glucose level,and obesity),factors on living styles(physical exercise,diet&chronic diseases),and genetic factor(ApoE),associated with CI onset.Conclusions:This study confirms the high prevalence of CI among the elderly population in the Shanghai urban in China,similar to previous epidemiologic studies in Western countries.The putative risk factors associated with CI merit further investigated. | Jun-Peng Zhuang Gang Wang Qi Cheng Li-Ling Wang Rong Fang Li-Hua Liu Ran Xiong Yu Zhang Ya-Xing Gui Wen-Yan Kang Hui-Dong Tang Sheng-Di Chen | 2012 | Translational Neurodegeneration2012，1，1： | 10 |
| 14 | Study on the mechanism of copper-ammonia complex decomposition in struvite formation process and enhanced ammonia and copper removal显示文摘Heavy metals and ammonia are difficult to remove from wastewater,as they easily combine into refractory complexes.The struvite formation method(SFM) was applied for the complex decomposition and simultaneous removal of heavy metal and ammonia.The results indicated that ammonia deprivation by SFM was the key factor leading to the decomposition of the copper-ammonia complex ion.Ammonia was separated from solution as crystalline struvite,and the copper mainly co-precipitated as copper hydroxide together with struvite.Hydrogen bonding and electrostatic attraction were considered to be the main surface interactions between struvite and copper hydroxide.Hydrogen bonding was concluded to be the key factor leading to the co-precipitation.In addition,incorporation of copper ions into the struvite crystal also occurred during the treatment process. | Cong Peng Liyuan Chai Chongjian Tang Xiaobo Min Yuxia Song Chengshan Duan Cheng Yu | 2017 | Journal of Environmental Sciences2017，29，1： | 9 |
| 15 | Association of a SLC30A8 Genetic Variant with Monotherapy of Repaglinide and Rosiglitazone Effect in Newly Diagnosed Type 2 Diabetes Patients in China显示文摘Objective To investigate a potential relationship between Solute carrier family 30 (zinc transporter) member 8 (SLC30A8) rs13266634 variant and efficacy of rosiglitazone or repaglinide in treating newly diagnosed Chinese type 2 diabetes patients.Methods A total of 209 diabetic patients without any antihyperglycemic history were recruited and treated with repaglinide or rosiglitazone randomly for 48 weeks (104 and 105 patients,respectively).Anthropometric measurements and clinical laboratory tests were carried out before and after the treatment.An non-synonymous variant rs13266634 was genotyped by matrix-assisted laser desorption ionization-time of flight mass spectroscopy.Results Ninety-one patients in repaglinide group and ninety-three patients in rosiglitazone group completed the study.Δ value of homeostasis model assessment of beta cell function (HOMA-B)and Δ value of fasting proinsulin levels were statistically significant between three genotype groups (P=0.0149 and 0.0246,respectively) after rosiglitazone treatment.However,no genotype association was observed in the repaglinide or rosiglitazone group with other parameters.Conclusion The SLC30A8 variant was associated with the efficacy of insulin sensitizer monotherapy on insulin secretion in patients with newly diagnosed type 2 diabetes mellitus in Shanghai,China. | JIANG Feng LI Qing HU Cheng ZHANG Rong WANG Cong Rong YU Wei Hui LU Jing Yi TANG Shan Shan BAO Yu Qian XIANG Kun San JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 8 |
| 16 | An in vitro and finite element study of load redistribution in the midfoot显示文摘A good knowledge of midfoot biomechanics is important in understanding the biomechanics of the entire foot,but it has never been investigated thoroughly in the literature.This study carried out in vitro experiments and finite element analysis to investigate the midfoot biomechanics.A foot-ankle finite element model simulating the mid-stance phase of the normal gait was developed and the model validated in in vitro experimental tests.Experiments used seven in vitro samples of fresh human cadavers.The simulation found that the first principal stress peaks of all midfoot bones occurred at the navicular bone and that the tensile force of the spring ligament was greater than that of any other ligament.The experiments showed that the longitudinal strain acting on the medial cuneiform bone was-26.2±10.8μ-strain,and the navicular strain was-240.0±169.1μ-strain along the longitudinal direction and 65.1±25.8μ-strain along the transverse direction.The anatomical position and the spring ligament both result in higher shear stress in the navicular bone.The load from the ankle joint to five branches of the forefoot is redistributed among the cuneiforms and cuboid bones.Further studies on the mechanism of loading redistribution will be helpful in understanding the biomechanics of the entire foot. | NIU Wen Xin TANG Ting Ting ZHANG Ming JIANG Cheng Hua FAN Yu Bo | 2014 | Science China(Life Sciences)2014，57，12： | 8 |
| 17 | Efficacy of thymosin alpha-1 and interferon alpha in treatment of chronic viral hepatitis B:A randomized controlled study显示文摘AIM: To observe the efficiency and safety of thymosin-α1 treatment in patients with hepatitis B e antigen (HBeAg) and HBV DNA positive chronic hepatitis. METHODS: Sixty-two patients were randomly divided into groups A and B. The patients in group A received subcutaneous injection of 1.6 mg thymosin-α1, twice a week (T-α1 group) for six months, and the patients in group B received 5 MU interferon alpha (IFN-α) each day for fifteen days, then three times weekly (IFN-α group) for six months. The results between two groups treated with and the group untreated with IFN-α which was followed up for 12 mo (historical control group consisting of 30 patients) were compared, and three groups were comparable between each other (P > 0.05) at baseline (age, sex, clinical history, biochemical, and serological parameters). RESULTS: At the end of treatment, complete response, which was defined as alanine aminotransferase (ALT) normalization and HBV DNA and HBeAg loss, occurred in 9 of 29 (31.0%) patients in the T-α1 group and in 15 of 33 (45.5%) patients in the IFN-α group (c2 = 1.36, P >0.05). After a follow-up period of six months, a complete response was observed in 14 of 29 (48.3%) patients in the T-α1 group and in 9 of 33 (27.3%) patients in the IFN-α group (c2 = 2.93, P > 0.05). Compared with the results observed in the historical control (HC) group untreated with IFN-α which was followed up for 12 mo, the rate of complete response was significantly higher in IFN-α group at the end of therapy (1 of 30 vs 15 of 33, c2 = 14.72, P < 0.001) and in the T-α1 group at the end of follow-up (1 of 30 vs 14 of 29, c2 = 15.71, P < 0.001). In T-α1 and IFN-α treatment groups, the area under (the plasma concentration time) curve (AUC) of negative HBV DNA and HBeAg was 34%, 17%, 31% and 19% smaller than that in the HC group. By the end of the follow- up period, the proportions of ALT normalization and negative HBV DNA in the T-α1 group were significantly higher than those in the IFN-α and HC groups. The odds of ALT normalization and negative HBV DNA at the end of the follow-up was three-fold higher in the T-α1 group than in the IFN-α group. Unlike IFN-α, T-α1 was well tolerated by all patients, and no side effects appeared in T-α1 group.CONCLUSION: The results suggest that a 6-mo course of T-α1 therapy is effective and safe in patients with chronic hepatitis B. T-α1 is able to reduce HBV replication in patients with chronic hepatitis B. Furthermore, T-α1 is better tolerated than IFN-α and can gradually induce more sustained ALT normalization and HBV DNA and HBeAg loss. However, a response rate of 48.3% is still less ideal. A more effective therapeutic approach warrants further study. | Jing You Lin Zhuang Hong-Ying Cheng Shou-Ming Yan Lan Yu Jun-Hua Huang Bao-Zhang Tang Meng-Ling Huang Yong-Liang Ma Virasakdi Chongsuvivatwong Hutcha Sriplung Alan Geater Yan-Wei Qiao Rong-Xue Wu | 2006 | World Journal of Gastroenterology2006，12，41： | 8 |
| 18 | Controllable optical activity with non-chiral plasmonic metasurfaces显示文摘Optical activity is the rotation of the plane of linearly polarized light along the propagation direction as the light travels through optically active materials.In existing methods,the strength of the optical activity is determined by the chirality of the materials,which is difficult to control quantitatively.Here we numerically and experimentally investigated an alternative approach to realize and control the optical activity with non-chiral plasmonic metasurfaces.Through judicious design of the structural units of the metasurfaces,the right and left circular polarization components of the linearly polarized light have different phase retardations after transmitting through the metasurfaces,leading to large optical activity.Moreover,the strength of the optical activity can be easily and accurately tuned by directly adjusting the phase difference.The proposed approach based on non-chiral plasmonic metasurfaces exhibits large optical activity with a high controllable degree of freedom,which may provide more possibilities for applications in photonics. | Ping Yu Jianxiong Li Chengchun Tang Hua Cheng Zhaocheng Liu Zhancheng Li Zhe Liu Changzhi Gu Junjie Li Shuqi Chen Jianguo Tian | 2016 | Light(Science & Applications)2016，5，1： | 8 |
| 19 | Interferon-armed RBD dimer enhances the immunogenicity of RBD for sterilizing immunity against SARS-CoV-2显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused a global crisis,urgently necessitating the development of safe,efficacious,convenient-to-store,and low-cost vaccine options.A major challenge is that the receptor-binding domain(RBD)-only vaccine fails to trigger long-lasting protective immunity if used alone for vaccination. | Shiyu Sun Yueqi Cai Tian-Zhang Song Yang Pu Lin Cheng Hairong Xu Jing Sun Chaoyang Meng Yifan Lin Haibin Huang Fang Zhao Silin Zhang Yu Gao Jian-Bao Han Xiao-Li Feng Dan-Dan Yu Yalan Zhu Pu Gao Haidong Tang Jincun Zhao Zheng Zhang Jiaming Yang Zhenxiang Hu Yang-Xin Fu Yong-Tang Zheng Hua Peng | 2021 | Cell Research2021，31，9： | 7 |
| 20 | Tetramethylpyrazine protects lymphocytes from radiationinduced apoptosis through nuclear factor-κB显示文摘AIM: Radiation induces an important apoptosis response in irradiated organs. The objective of this study was to investigate the radioprotective effect of tetramethylpyrazine(TMP) on irradiated lymphocytes and discover the possible mechanism of protection. METHOD: Lymphocytes were pretreated for 12 h with TMP(25-200 μmol·L-1) and then exposed to 4 Gy radiation. Cell apoptosis and the signaling pathway were analyzed. RESULTS: Irradiation increased cell death, DNA fragmentation, activated caspase activation and cytochrome c translocation, downregulated B-cell lymphoma 2(Bcl-2) and up-regulated Bcl-2-associated X protein(Bax). Pretreated with TMP significantly reversed this tendency. Several anti-apoptotic characteristics of TMP, including the ability to increase cell viability, inhibit caspase-9 activation, and upregulate Bcl-2 and down-regulate Bax in 4Gy-irradiated lymphocytes were determined. Signal pathway analysis showed TMP could translate nuclear factor-κB(NF-κB) from cytosol into the nucleus. CONCLUSION: The results suggest that TMP had a radioprotective effect through the NF-κB pathway to inhibit apoptosis, and it may be an effective candidate for treating radiation diseases associated with cell apoptosis. | WANG Xiao-Yan MA Zeng-Chun WANG Yu-Guang TAN Hong-Ling XIAO Cheng-Rong LIANG Qian-De TANG Xiang-Lin CHENG Yu GAO Yue | 2014 | Chinese Journal of Natural Medicines2014，12，10： | 7 |
您所在的机构暂未开通此部分的功能，请联系管理员