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| 1 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 2 | Wx^lv、the Ancestral Allele of Rice Waxy Gene显示文摘In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked rice have been elucidated.However,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain unclear.Here we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose molecules.We demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional sites.These data indicate that the Wx^lv allele played an important role in artificial selection and domestication.The findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice. | Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu | 2019 | Molecular Plant2019，12，8： | 22 |
| 3 | OsAM1 is required for leptotene-zygotene transition in rice显示文摘发生在成熟分裂的发作的事件充分没被阐明。在现在的学习, OsAM1 被基于地图的克隆在米饭(Oryza sativa L.) 识别。OsAM1, Arabidopsis SWI1 的一个相当或相同的事物和玉米 AM1,与一个卷卷领域把蛋白质编码为它的中央区域。在 Osam1 异种,花粉母亲房间在 leptotene 被逮捕,证明 OsAM1 为 leptotene-zygotene 转变被要求。Immunocytological 分析表明 OsAM1 在早前期作为 foci 存在我 meiocytes。很微弱的 OsREC8 foci 在 Osam1 异种坚持了,显示 OsAM1 没为 OsREC8 的起始的 meiotic 招募被要求。当 OsAM1 不在时,包括 PAIR2, ZEP1 和 OsMER3,许多另外的批评 meiotic 部件不能正确地被安装到染色体上。在 pair2, Osmer3 和 zep1 异种,相反, OsAM1 能通常被装载,建议 OsAM1 起造在成熟分裂的开始的合适的染色体结构里的一个基本作用。 | Lixiao Che Ding Tang Kejian Wang Mo Wang Keming Zhu Hengxiu Yu Minghong Gu Zhukuan Cheng | 2011 | Cell Research2011，21，4： | 4 |
| 4 | Centromere inactivation in a dicentric rice chromosome during sexual reproduction显示文摘T0135 is a variant selected from the progeny of a rice line telotrisomic for the short arm of chromosome 11(2n+11S·).Fluorescent in situ hybridization(FISH)results indicated that T0135 contained two telocentric chromosomes,which have two centromere-specific molecular markers(5S rDNA)for chromosome 11;thus T0135 is a newly-described rice chromosome variant with two dicentric chromosomes,named 22+11L·+11L·+11S·11S·+11S·11S·(22 represents the 22 chromosomes excluding chromosome 11 in the rice genome,'·'represents the centromere).To investigate the genetic stability of the rice dicentric chromosomes during sexual reproduction,we observed the chromosome types in the progeny.Ninety-four percent of the progeny had the same chromosome type as the parental line.This result indicates that the dicentric chromosomes are mostly stable during mitosis and meiosis.Immunofluorescence analysis for centromere specific histone H3(CENH3)revealed that only one centromere is active and the other centromere is inactivated in the rice dicentric chromosomes. | GONG ZhiYun XUE Chao LIU XiuXiu ZHANG MingLiang ZHOU Yong YU HengXiu GU MingHong | 2013 | Chinese Science Bulletin2013，58，36： | 3 |
| 5 | Generation of selectable marker-free transgenic rice resistant to chewing insects using two co-transformation systems显示文摘生产可选没有标记(SMF ) 对咀嚼昆虫抵抗的转基因的米饭,杆菌 thuringiensis cryIA (c) 基因(Bt ) 被使用二个调停 Agrobacterium 的合作转变系统介绍进二个精英装饰用的梨树米饭变化。一个系统与在一 Agrobacterium 紧张的单个微型双胞胎 T-DNA 二进制向量,它由二个分开的 T-DNA 区域组成,当其它包含可选的标记基因时,带 Bt 的, hygromycin 抵抗基因(HPT ) 。另外的系统在二种分开的 Agrobacterium 文化使用二分开的二进制向量,包含单个原生质标志上的 Bt 或 HPT 基因。怀有 Bt 和可选的标记基因的很多独立转基因的米饭线被获得。结果证明 Bt 基因和 HPT 基因的合作转变频率由由二个分开的二进制向量系统(4.52%) 比那使用微型双胞胎 T-DNA 向量系统(29.87%) 是高得多的。然而,从 co 转基因的植物(21.74%) 的后代获得的 SMF 转基因的米饭植物的频率为后者比那为微型双胞胎 T-DNA 向量系统是更低的(50 – 60%) 。ELISA 的数据暗示表示 Bt 蛋白质作为 0.025 – 被测定; 0.103% 总数长叶在转基因的植物的可溶的蛋白质。因此,没有可选的标记基因,转基因的米饭衬里的几精英被选择。在 vitro 并且在 vivo 昆虫生物鉴定的从两个的结果显示 SMF 转基因的米饭被显示对有斑纹的茎 borer 和米饭叶文件夹高度抵抗。而且处于没有使用的任何杀虫药剂的一个自然的地条件,所有转基因的大米植物被发现到别被大米叶文件夹伤害,而野类型严重被损害。 | Hengxiu Yu Quanhong Yao Ling Wang Zhipeng Zhao Zhiyun Gong Shuzhu Tang Qiaoquan Liu Minghong Gu | 2009 | Progress in Natural Science:Materials International2009，19，11： | 2 |
| 6 | Isolation and identification of the three rice monotelosomics显示文摘In order to obtain rice monotelosomic, the progeny of 24 telotrisomics, derived from an indica rice vari-ety, Zhongxian 3037, were screened. The variants that dif-fered morphologically from the diploids and the original primary trisomics as well as the telotrisomics were collected for cytological identification. The variants with 24 chromo-somes were selected according to the prometaphase chromo-somes. From these variants, three monotelosomics with one chromosome arm deletion in each were verified by fluores-cence in situ hybridization (FISH) using a rice centromeric BAC clone of 17p22 as a marker probe. The three monote-losomics were derived from telotrisomic 1S, 4L and 11L, re-spectively. Further identification was conducted on the pro-metaphase or pachytene chromosomes of the three variants, which were probed with the same centromeric BAC clone together with the corresponding chromosome arm specific makers, a0059H02 (on the short arm of chromosome 1), a0034E24 (on the long arm of chromosome 4), and a0071H11 (on the long arm of chromosome 11). The results indicated that the telocentric chromosomes in the three monotelosom-ics were derived from their respective corresponding telotrisomics. According to the telocentric chromosomes of the variants, they were monotelosomic 1S (one long arm of chromosome 1 was lost), monotelosomic 4L (one short arm of chromosome 4 was lost) and monotelosomic 11L (one short arm of chromosome 11 was lost), respectively. | YU Hengxiu GONG Zhiyun SU Yan GU Minghong | 2005 | Chinese Science Bulletin2005，50，19： | 2 |
| 7 | Generation of selectable marker-free soft transgenic rice with transparent kernels by downregulation of SSSⅡ-2显示文摘The amylose content(AC)of rice endosperm starch varies from 0 to 35%,and is associated with rice cooking and eating quality.Soft rice has low AC,generally between 6%and 15%,and its eating quality is high whether it is consumed hot or cold.However,the appearance quality of current soft rice cultivars needs to be improved,especially opaque endosperm.Conventional genetic engineering has improved some agronomic traits of soft rice varieties,but not endosperm appearance.In the present study,a RNAi construct of the soluble starch synthase II-2(SSSII-2)and the hygromycin phosphotransferase(HPT)gene were introduced into an elite japonica rice variety,Kangtiaowuyunjing(KWY8)by co-transformation.Several selectable marker-free(SMF)transgenic lines were obtained,and SSSII-2 expression was significantly downregulated in selected transgenic lines,resulting in lower AC of the endosperm.The physicochemical properties of the transgenic rice kernels,including gel consistency(GC)and rapid visco analyzer(RVA)profile,differed significantly from those of wild-type rice and were similar to those of a soft rice variety,Nanjing 46(NJ46).These findings indicate that the cooking,eating,and processing qualities of transgenic rice are comparable to those of NJ46.However,the transgenic rice endosperm retained a transparent appearance under low-moisture conditions.Thus,SMF SSSII-2 RNAi rice provides a resource for breeding soft rice with transparent endosperm. | Zhihao Xu Meimei Yu Youwei Yin Cenwen Zhu Wen Ji Changquan Zhang Qianfeng Li Honggen Zhang Shuzu Tang Hengxiu Yu Qiaoquan Liu | 2020 | The Crop Journal2020，8，1： | 2 |
| 8 | Quality character- istics and field performance of selectable marker-free trans- genic rice with antisense Wx gene and improved quality de- rived from the elite parents of hybrid indica rice显示文摘 | Yu Hengxiu Liu Qiaoquan Xu Li | 2009 | Journal of Cereal Science2009，50，3： | 1 |
| 9 | Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality显示文摘 | Qiaoquan Liu Zongyang Wang Xiuhua Chen Xiuling Cai Shuzhu Tang Hengxiu Yu Jinliu Zhang Menming Hong Minghong Gu | 2003 | Transgenic Research2003，，1： | 1 |
| 10 | ERECT PANICLE2 Encodes a Novel Protein That Regulates Panicle Erectness in Indica Rice显示文摘 | Zhu Keming Tang Ding Yan Changjie Chi Zhengchang Yu Hengxiu Chen Jianmin Liang Jiansheng Gu Minghong Cheng Zhukuan | 2010 | Genetics2010，，2： | 1 |
| 11 | Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality显示文摘 | Qiaoquan Liu Zongyang Wang Xiuhua Chen Xiuling Cai Shuzhu Tang Hengxiu Yu Jinliu Zhang Menming Hong Minghong Gu | 2003 | Transgenic Research2003，，1： | 1 |
| 12 | DsMTOPVIB Promotes Meiotic DNA Double-Strand Break Formation in Rice显示文摘 | Zhihui Xue Yafei Li LeiZhang Wenqing Shi Chao Zhang Mengshi Feng Fanfan Zhang Ding Tang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2016 | Molecular Plant2016，9，11： | 0 |
| 13 | Chromosome location of a gene for aroma in rice显示文摘Scented or aromatic rice(Oryza sativa L.)ishighly valued in many areas of the world,Thedevelopment of high yielding scented cultivarshas been limited because of lacking informationon the inheritance of scent such as linkage ofgenes for aroma with other genes.We reporthere our studies on the determination of | LI Xin GU Minghong CHENG Zhukuan and YU Hengxiu,Dept of Agro,Jiangsu Agri Coll,Yangzhou 225009,China | 1996 | Chinese Rice Research Newsletter1996，4，2： | 0 |
| 14 | Generating of rice OsCENH3-GFP transgenic plants and their genetic applications显示文摘In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exogenous genes in the transgenic plants was confirmed by PCR and Southern blotting. The transgenic plants grow normally during their whole life time, just like Zhongxian 3037. No significant defects were detected in either mitosis or meiosis of the transgenic plants. The overlapping of GFP signals and anti-CENH3 foci in both mitotic and meiotic cells from T0 and T1 generation plants indicated that GFP had been successfully fused with CENH3, so the GFP signals can well represent the CENH3 locations on each chromosome. To evaluate the applicability of the transgenic plants to other genetic studies, fluorescence in situ hybridization (FISH) using rice centromeric tandem repetitive sequence CentO as the probe was conducted on the zygotene chromosomes of pollen mother cells (PMCs). It has been revealed that the GFP signals are overlapping with CentO FISH signals, showing that CentO is one of the key elements constituting rice functional centromeres. Immunofluorescent staining using anti-α-tublin antibody and anti-PAIR2 antibody on the chromosomes during mitosis and meiosis stages of the transgenic plants further reveals that OsCENH3-GFP transgenic plants can be widely used for studying rice molecular biology, especially for tagging functional centromeres in both living cells and tissues. | YU HengXiu WANG Xin GONG ZhiYun TANG Ding GU MingHong CHENG ZhuKuan | 2008 | Chinese Science Bulletin2008，53，19： | 0 |
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