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| 1 | Matrix metalloproteinases contribute to kidney fibrosis in chronic kidney diseases显示文摘Matrix metalloproteinases(MMPs) are members of the neutral proteinase family. They were previously thought to be anti-fibrotic because of their ability to degrade and remodel of extracellular matrix. However, recent studies have shown that MMPs are implicated in initiation and progression of kidney fibrosis through tubular cell epithelial–mesenchymal transition(EMT) as well as activation of resident fibroblasts, endothelial-mesenchymal transition(Endo MT) and pericyte-myofibroblast transdifferentiation. Interstitial macrophage infiltration has also been shown to correlate with the severity of kidney fibrosis in various chronic kidney diseases. MMPs secreted by macrophages, especially MMP-9, hasbeen shown by us to be profibrotic by induction of tubular cells EMT. EMT is mainly induced by transforming growth factor-β(TGF-β). However, MMP-9 was found by us and others to be up-regulated by TGF-β1 in kidney tubular epithelial cells and secreted by activated macrophages, resulting in EMT and ultimately kidney fibrosis. Therefore, MMP-9 may serve as a potential therapeutic target to prevent kidney fibrosis in chronic kidney disease. This review, by a particular focus on EMT, seeks to provide a comprehensive understanding of MMPs, especially MMP-9, in kidney fibrosis. | Hong Zhao Yanting Dong Xinrui Tian Thian Kui Tan Zhuola Liu Ye Zhao Yun Zhang David CH Harris Guoping Zheng | 2013 | World Journal of Nephrology2013，2，3： | 21 |
| 2 | De novo assembly of a Chinese soybean genome显示文摘Soybean was domesticated in China and has become one of the most important oilseed crops. Due to bottlenecks in their introduction and dissemination, soybeans from different geographic areas exhibit extensive genetic diversity. Asia is the largest soybean market; therefore, a high-quality soybean reference genome from this area is critical for soybean research and breeding.Here, we report the de novo assembly and sequence analysis of a Chinese soybean genome for 'Zhonghuang 13' by a combination of SMRT, Hi-C and optical mapping data. The assembled genome size is 1.025 Gb with a contig N50 of 3.46 Mb and a scaffold N50 of 51.87 Mb. Comparisons between this genome and the previously reported reference genome(cv. Williams82) uncovered more than 250,000 structure variations. A total of 52,051 protein coding genes and 36,429 transposable elements were annotated for this genome, and a gene co-expression network including 39,967 genes was also established. This high quality Chinese soybean genome and its sequence analysis will provide valuable information for soybean improvement in the future. | Yanting Shen Jing Liu Haiying Geng Jixiang Zhang Yucheng Liu Haikuan Zhang Shilai Xing Jianchang Du Shisong Ma Zhixi Tian | 2018 | Science China(Life Sciences)2018，61，8： | 11 |
| 3 | Effectiveness of Jinying capsule on pelvic inflammatory disease in patients with symptom pattern of damp and heat accumulation: a double-blinded, multicenter, randomized, placebo-controlled clinical trial显示文摘OBJECTIVE: To evaluate the therapeutic effectiveness and safety of Jinying capsule on pelvic inflammatory disease(PID) in patients with symptoms identified as the pattern of damp and heat accumulation in terms of Traditional Chinese Medicine(TCM).METHODS: We conducted a double-blinded, multicenter, randomized, placebo-controlled clinical trial which included 155 patients diagnosed with PID and identified as symptom pattern of damp and heat accumulation. They were randomly divided into experimental group(n = 78) and control group(n = 77) according to a random number table. The treatment lasted for a period of 28 d. The experimental group was given Jinying capsules and oral levofloxacin plus oral metronidazole for first 7 d.They continued with Jinying capsules and levofloxacin placebo and metronidazole placebo for another 7 d. For the remaining 14 d, they continued with Jinying capsules only. Whereas, the control group was treated with oral levofloxacin and metronidazole and Jinying capsule placebo for the first 14 d in the same way as the experimental group and then continued with Jinying capsule placebo only for the remaining 14 d. The clinical efficacy was assessed using McCormack scale, TCM symptom pattern scores, physicochemical indexes including white blood cell and erythrocyte sedimentation rate, C-reaction protein, smear of vaginal discharge,and pelvic ultrasound.RESULTS: Comparing McCormack scale between both groups after treatment, the difference in curative effect between both groups was significant (P = 0.0269). The cure rate of the experimental group and control group is 76.32% and 59.46% respectively at week 4. Comparing TCM symptom pattern scores between both groups before and after treatment, the differences in total effective rate were both significant(P < 0.05). The curative effect rate of experimental group is 2.63% and 13.70% of the control group at week 1(P = 0.0131), and73.33% of the experimental group and 56.94% of the control group at week 4(P = 0.0369). No significant differences were found between the two groups on the Physicochemical indexes(all P >0.05). No adverse events or reactions occurred in the experimental groups.CONCLUSION: Jinying capsule can reduce the dosage of antibiotics needed for PID treatment, and improve the symptoms in PID patients. | Yang Yan Lu Yao Zhou Deping Pei Fenglan Li Qin Chen Shuqiong Wang Qiufeng Wang Yanting Jin Zhe Liu Yanxia | 2020 | Journal of Traditional Chinese Medicine2020，40，3： | 10 |
| 4 | Highly efficient and precise base editing in discarded human tripronuclear embryos显示文摘 | Guanglei Li Yajing Liu Yanting Zeng Jianan Li Lijie Wang Guang Yang Dunjin Chen Xiaoyun Shang Jia Chen Xingxu Huang Jianqiao Liu | 2017 | Protein & Cell2017，8，10： | 8 |
| 5 | CuFe2O4@GO nanocomposite as an effective and recoverable catalyst of peroxymonosulfate activation for degradation of aqueous dye pollutants显示文摘Recently,heterogeneous activation of peroxymonosulfate(PMS) to oxidatively degrade organic pollutants has been a hotspot.In the present work,copper ferrite-graphite oxide hybrid(CuFe2 O4@GO)was prepared and used as catalyst to activate PMS for degradation of methylene blue(MB) in aqueous solution.A high degradation efficiency(93.3%) was achieved at the experimental conditions of20 mg/L MB,200 mg/L CuFe2 O4@GO,0.8 mmol/L PMS,and 25℃temperature.Moreover,CuFe2 O4@GO showed an excellent reusability and stability.The effects of various operational parameters including pollutant type,solution pH,catalyst dosage,PMS dosage,pollutant concentration,temperature,natural organic matter(NOM),and inorganic anions on the catalytic degradation process were comprehensively investigated and elucidated.The further mechanistic study revealed the Cu(Ⅱ)/Cu(Ⅰ) redox couple on CuFe2 O4@GO played the dominant role in PMS activation,where both hydroxyl and sulfate radicals were generated and proceeded the degradation of pollutants.In general,CuFe2 O4@GO is a promising heterocatalyst for PMS-based advanced oxidation processes(AOPs) in wastewater treatment. | Xiaoman Lei Menghan You Fei Pan Min Liu Peng Yang Dongsheng Xia Qiang Li Yanting Wang Jie Fu | 2019 | Chinese Chemical Letters2019，30，12： | 8 |
| 6 | Efficacy and advantages of modified Traditional Chinese Medicine treatments based on'kidney reinforcing'for chronic aplastic ane-mia:a randomized controlled clinical trial显示文摘OBJECTIVE:To compare the efficacy of modified treatments based on 'kidney reinforcing' in the management of chronic aplastic anemia(CAA),and explore their advantages and specialties.METHODS:One hundred and eleven patients with CAA were randomly divided into three groups:kidney reinforcing alone(KA),'kidney reinforcing and Qi tonifying'(KQ),and 'kidney reinforcing and blood circulation invigorating'(KC).Normal and positive control groups were also formed.All patients were treated for 6 months(two courses).Hemograms,Traditional Chinese Medicine(TCM) syndrome scores,and therapeutic effects were assessed,and changes in T-lymphocyte subsets,regulatory T cells and cytokines were detected.RESULTS:The KQ and KC groups had lower TCM syndrome scores than the positive control group after 6 months(P < 0.05).The KQ group had a higher overall efficacy than the positive control group after 3 months(P < 0.05),while platelet counts increased in the KC group after 6 months(P < 0.05).CD3+ T-lymphocyte ratios decreased only in the KQ group,while CD3 + CD4 + CD8-Tlymphocytes increased only in the KC group after 6 months(P <0.05).Levels of interferon-γ,tumor necrosis fac-tor-α,interleukin(IL)-2 and IL-6 decreased and levels of IL-4 and IL-10 increased in all treated groups after 6 months.Levels of IL-6 in the KQ and KC groups were lower than those in the positive control group(P < 0.05).CONCLUSION:Treatments based on kidney reinforcing have a rebalancing effect on cytotoxic and T helper cells,and regulate expression of interferon-γ,IL-2,IL-6 and IL-4.KQ may be more effective in treating CAA,and KC may have an advantage in platelet recovery. | Wu Dijiong Shen Yiping Ye Baodong Fang Bingmu Lin Shengyun Chen Zhilu Jiang Huifang Feng Changwei He Lüyuan Gao Yanting Liu Yonglin Liu Yonghua Zhu Jiajia Wu Liqiang Shao Keding Zhou Yuhong | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 6 |
| 7 | Update soybean Zhonghuang 13 genome to a golden reference显示文摘Dear Editor,Soybean is one of the most important crops worldwide.A high-quality reference genome will facilitate its functional analysis and molecular breeding (Wang and Tian,2015).Previously,we de novo assembled a high-quality Chinese soybean genome Gmax_ZH13 (Shen et al.,2018,Yang and Huang,2018).However,due to technical limitations at the time when we generated Gmax_ZH13,a large number of small contigs were not anchored onto chromosomes.Therefore,we here build a new golden reference genome for Zhonghuang 13 consisting of 20 nearly complete chromosomes by adding more single-molecule real time (SMRT) sequencing reads.Furthermore,we add large RNA-seq and smRNA-seq datasets for improving the annotation of its protein coding genes. | Yanting Shen Huilong Du Yucheng Liu Lingbin Ni Zheng Wang Chengzhi Liang Zhixi Tian | 2019 | Science China(Life Sciences)2019，62，9： | 6 |
| 8 | Ecological indicators showing the succession of macrofauna communities in Sonneratia apetala artificial mangrove wetlands on Qi'ao Island at Zhuhai, South China显示文摘The Sonneratia apetala artificial mangroves in the intertidal zone of Da Wei Bay at Qi'ao Island of Zhuhai, South China were chosen as the macrofauna succession plots while bare tidal flats of the same size were established as control plots in surrounding interference-free areas. Conventional change indicators of community structure, such as biomass and biodiversity, and indicators, such as exergy and specific exergy, which reflect the information change of overall communities, were used to analyze the succession of macrofauna communities in S. apetala artificial mangroves. The similarities and differences in variation tendency of the different ecological indicators and their reflected ecological principles were compared. The results showed that from D-1 to D-1275 after planting S. apetala, the biomass of the macrofauna communities first increased, which was then followed by an increase in the network relationship between the macrofauna communities(analysis of the Pielou evenness index and Shannon-Wiener diversity index). The system information(specific exergy) increased the slowest. Between D-1460 and D-2370 after planting S. apetala, there was a decrease in biomass, network structure, and system information in the succession plots. After the decrease in the system information(the specific exergy), there was a decline in the network relationships(Pielou evenness index and Shannon-Wiener diversity index). Biomass was the last indicator to decrease. The similarities and differences among the different ecological indicators varied during the succession process, which reflected the relativity and differences among the indicators. This study suggested that, although the species diversity index can be an effective indicator of two types of changes(network structure and system information), it was quite clear that species diversity measurement was not suitable for expressing the changes in biomass during the succession process. While exergy and specific exergy can provide useful information about the structural development of communities, they cannot identify the information state of the system. Therefore, when evaluating macrofaunal succession in S. apetala artificial mangrove wetlands, it would be better to apply a number of different ecological indicators, rather than just one single indicator. | TANG Yijie FANG Zhanqiang ZHANG Zaiwang ZHONG Yanting CHEN Kang LIU Benhan FAN Junjie XIONG Tiantian LU Xiaosheng | 2014 | Acta Oceanologica Sinica2014，33，8： | 6 |
| 9 | Atorvastatin attenuates surgery-induced BBB disruption and cognitive impairment partly by suppressing NF-κB pathway and NLRP3 inflammasome activation in aged mice显示文摘In clinic,perioperative neurocognitive disorder is becoming a common complication of surgery in old patients.Neuroinflammation and blood-brain barrier(BBB)disruption are important contributors for cognitive impairment.Atorvastatin,as a strong HMG-CoA reductase inhibitor,has been widely used in clinic.However,it remains unclear whether atorvastatin could prevent anesthesia and surgery-induced BBB disruption and cognitive injury by its anti-inflammatory property.In this study,aged C57BL/6J mice were used to address this question.Initially,the mice were subject to atorvastatin treatment for 7 days(10 mg/kg).After a simple laparotomy under 1.5%isoflurane anesthesia,Morris water maze was performed to assess spatial learning and memory.Western blot analysis,immunohistochemistry,and enzyme-linked immunosorbent assay were used to examine the inflammatory response,BBB integrity,and cell apoptosis.Terminal-deoxynucleotidyl transferase mediated nick end labeling assay was used to assess cell apoptosis.The fluorescein sodium and transmission electron microscopy were used to detect the permeability and structure of BBB.The results showed that anesthesia and surgery significantly injured hippocampal-dependent learning and memory,which was ameliorated by atorvastatin.Atorvastatin could also reverse the surgery-induced increase of systemic and hippocampal cytokines,including IL-1β,TNF-α,and IL-6,accompanied by inhibiting the nuclear factor kappa-B(NF-κB)pathway and Nucleotide-Binding Oligomerization Domain,or Leucine Rich Repeat and Pyrin Domain Containing 3(NLRP3)inflammasome activation,as well as hippocampal neuronal apoptosis.In addition,surgery triggered an increase of BBB permeability,paralleled by a decrease of the ZO-1,occludin,and Claudin 5 proteins in the hippocampus.However,atorvastatin treatment could protect the BBB integrity from the impact of surgery,by up-regulating the expressions of ZO-1,occludin,and Claudin 5.These findings suggest that atorvastatin exhibits neuroprotective effects on cognition in aged mice undergoing surgery. | Pengfei Liu Quansheng Gao Lei Guan Yanting Hu Jingwen Jiang Teng Gao Weixuan Sheng Xinying Xue Hui Qiao Tianzuo Li | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 6 |
| 10 | New molecular method to detect denitrifying anaerobic methane oxidation bacteria from different environmental niches显示文摘The denitrifying anaerobic methane oxidation is an ecologically important process for reducing the potential methane emission into the atmosphere.The responsible bacterium for this process was Candidatus Methylomirabilis oxyfera belonging to the bacterial phylum of NC10.In this study,a new pair of primers targeting all the five groups of NC10 bacteria was designed to amplify NC10 bacteria from different environmental niches.The results showed that the group A was the dominant NC10 phylum bacteria from the sludges and food waste digestate while in paddy soil samples,group A and group B had nearly the same proportion.Our results also indicated that NC10 bacteria could exist in a high p H environment(pH 9.24)from the food waste treatment facility.The Pearson relationship analysis showed that the p H had a significant positive relationship with the NC10 bacterial diversity(p<0.05).The redundancy analysis further revealed that the p H,volatile solid and nitrite nitrogen were the most important factors in shaping the NC10 bacterial structure(p=0.01)based on the variation inflation factors selection and Monte Carlo test(999 times).Results of this study extended the existing molecular tools for studying the NC10 bacterial community structures and provided new information on the ecological distributions of NC10 bacteria. | Sai Xu Wenjing Lu Farooq Mustafa Muhammad Yanting Liu Hanwen Guo Ruihong Meng Hongtao Wang | 2018 | Journal of Environmental Sciences2018，30，3： | 4 |
| 11 | Mechanism underlying the retarded nuclear translocation of androgen receptor splice variants显示文摘As shown in our previous study, two alternatively spliced androgen receptor(AR) variants, which are exclusively expressed in the granulosa cells of patients with polycystic ovary syndrome, exhibit retarded nuclear translocation compared with wild-type AR. However, researchers have not yet determined whether these abnormalities correlate with heat shock protein 90(HSP90)and importin α(the former is a generally accepted co-chaperone of AR, and the latter is a component of classical nuclear import complexes). Here, these two variants were mainly retained in cytoplasm with HSP90 and importin α in the presence of dihydrotestosterone(DHT), and their levels in nucleus were significantly reduced, according to the immunofluorescence staining. The binding affinity of two AR variants for importin α was consistently decreased, while it was increased in WT-AR following DHT stimulation, leading to reduced nuclear import, particularly for the insertion-AR(Ins-AR). However, the binding affinities of two AR variants for HSP90 were increased in the absence of DHT compared with WT-AR, which functioned to maintain spatial structural stability, particularly for the deletion-AR(Del-AR). Therefore, the retarded nuclear translocation of two AR variants is associated with HSP90 and importin α, and the abnormal binding affinities for them play critical roles in this process. | Ye Liu Yinyu Wang Fangfang Wang Jiexue Pan Jingjing Xu Jingyi Li Chengliang Zhou Guolian Ding Yanting Wu Xinmei Liu Jianzhong Sheng Hefeng Huang | 2019 | Science China(Life Sciences)2019，62，2： | 4 |
| 12 | Synthesis, characteristics of hierarchical EU-1 zeolite for xylene isomerization probe reaction显示文摘Introduced a method of synthesizing hierarchical EU-1 zeolite with organosilanes as additive, and studied the influences of following different kinds of organosilanes on the synthesis of hierarchical EU-1 zeolite: γ-glycidoxy propyl trimethoxy silane(GPTMS), N-β-(aminoethyl)-γ-aminopropyl methyl dimethoxyl silane(APAEDMS),and N-(β-aminoethyl)-γ-aminopropyl dimethoxyl(ethyoxyl) silane(TMPED). The hierarchical EU-1 samples were characterized by XRD, SEM, N_2 adsorption, FT-IR and NH_3-TPD to analyze the crystallinity, morphology, surface area, pore size distribution and acidity. The results showed that hierarchical EU-1 zeolites were successfully synthesized; organosilanes have great influence on crystal morphology of EU-1 zeolites; the exterior surface area of hierarchical EU-1 zeolite, which synthesized with organosilanes(APAEDMS) adding into synthesis system, increased by 62.1% and mesopore volume increased by 129.1% compared with conventional EU-1 zeolites, thus can reduce the diffusional restriction markedly in catalytic reaction. The catalytic performance of hierarchical EU-1zeolites were evaluated in m-xylene isomerization on fixed bed reactor. The catalytic data showed that the isomerization activity PX/X of the hierarchical EU-1 zeolites reached around 24.09% in theoretical thermodynamic equilibrium from 23.83%, and the selectivity of C_8 aromatic hydrocarbon increased from 75.16% to 84.87%. The conversion of p-xylene increased from 16.30% to 18.41%. | Xiaofeng Li Pengchao Ren Yanting Zhang Xiaozhen Liu Xiaotao Sun Meng Gao Miaojuan Jia Zhiping Lü Tao Dou | 2016 | Chinese Journal of Chemical Engineering2016，24，11： | 4 |
| 13 | Fabrication of gelatin methacryloyl hydrogel microneedles for transdermal delivery of metformin in diabetic rats显示文摘Injection therapy for diabetes has poor patient compliance,and the pain occurring at the site of subcutaneous injections causes significant inconvenience to diabetic patients.In this work,to demonstrate the benefits of an alternative drug delivery technique that overcomes these issues,methacrylated gelatin hydrogel-forming microneedles integrated with metformin were developed to adjust blood glucose levels in diabetic rats.Gelatin methacryloyl microneedles(GelMA-MNs)with different degrees of substitution were successfully prepared by a micro-molding method.The resultant GelMA-MNs exhibited excellent mechanical properties and moisture resistance.Metformin,an anti-diabetic drug,was further encapsulated into the GelMA-MNs,and its release rate could be controlled by the three-dimensional cross-linked network of microneedles,thereby exhibiting sustained drug release behaviors in vitro and implying a better therapeutic effect compared with that of subcutaneous injection in diabetic rats.The drug release period could be significantly prolonged by improving the cross-link density of GelMA-MNs.The results of hypoglycemic effect evaluation show that the application of GelMA-MNs for transdermal delivery in diabetic rats has promising benefits for diabetes treatment. | Zhiyong Zeng Guohua Jiang Tianqi Liu Gao Song Yanfang Sun Xueya Zhang Yanting Jing Mingjia Feng Yufei Shi | 2021 | Bio-Design and Manufacturing2021，4，4： | 4 |
| 14 | Platelet-mediated adhesion facilitates leukocyte sequestration in hypoxia-reoxygenated microvessels显示文摘Leukocyte transendothelial migration and sequestration are two distinct outcomes following leukocyte adhesion to endothelium during ischemia-reperfusion injury, in which platelets may play a pivotal role. In the present study, we established an in vitro hypoxia-reoxygenation model to mimic ischemia-reperfusion injury and found platelet pre-incubation significantly increased leukocyte adhesion to endothelial cells after hyoxia-reoxygenation(over 67%). Blockade of endothelial-cell-expressed adhesion molecules inhibited leukocyte direct adhesion to endothelial cells, while platelet-mediated leukocyte adhesion was suppressed by blockade of platelet-expressed adhesion molecules. Further experiments revealed platelets acted as a bridge to mediate leukocyte adhesion, and platelet-mediated adhesion was the predominant pattern in the presence of platelets. However, platelet pre-incubation significantly suppressed leukocyte transendothelial migration after hypoxia-reoxygenation(over 31%), which could be aggravated by blockade of endothelial-cell-expressed adhesion molecules, but alleviated by blockade of platelet-expressed adhesion molecules. This would indicate that platelet-mediated adhesion disrupted leukocyte transendothelial migration. An in vivo mesenteric ischemia-reperfusion model demonstrated leukocyte transfusion alone caused mild leukocyte adhesion to reperfused vessels and subsequent leukocyte infiltration, while simultaneous leukocyte and platelet transfusion led to massive leukocyte adhesion and sequestration within reperfused microvessels. Our studies revealed platelets enhanced leukocyte adhesion to endothelial cells, but suppressed leukocyte transendothelial migration. Overall, this leads to leukocyte sequestration in hypoxia-reoxygenated microvessels. | Senfeng Zheng Yanting Cao Wenjian Zhang Honglin Liu Jia You Yiqing Yin Jinning Lou Chenghui Li | 2016 | Science China(Life Sciences)2016，59，3： | 3 |
| 15 | Characterization of Electroless Ni-P Deposits on Surface of AZ91D Magnesium Alloy显示文摘Characterization of electroless nickel deposits on magnesium alloys was investigated by X-ray diffraction(XRD),scanning electron microscope(SEM),energy dispersive spectrometer(EDS)and electrochemical potentiodynamic polarization analysis.The result of XRD showed that the electroless nickel deposits on magnesium alloys were composed mostly of amorphous Ni-P and partially of microcrystalline nickel.The phosphorus content in the Ni-P deposits was 10.8%by the calculation of EDS.SEM micrographs showed that morphology of electroless nickel deposits on magnesium alloys was a typical cellular structure,and the growth of cellular structure was influenced by surface condition of substrate.Zinc layer between substrate and electroless nickel deposits was not found by the results of SEM and EDS,which indicated that the zinc layer on the surface of magnesium alloy was very thin,and the zinc would dissolved while being immersed in electroless nickel plating solution.The thin zinc layer was almost dissolved completely before Ni-P plating.The result of electrochemical potentiodynamic polarization analysis showed that the corrosion potential Ecorr of electroless nickel deposits in 3.5%NaCl solution was about-0.53 V,which was 1 V higher than that of magnesium alloys substrate.And corrosion current density of Ni-P deposits in 3.5%NaCl solution was much smaller than that of magnesium alloys substrate at the same potential value,which indicated that corrosion resistance of electroless Ni-P deposits was excellent.Discoloration time of concentrated nitric acid test for electroless nickel deposits on magnesium alloys was 445 s,which also indicated excellent corrosion resistance. | Li Jiansan Guo Yanting Liu Junquan | 2012 | 稀有金属材料与工程2012，41，S1： | 3 |
| 16 | SPDEF suppresses head and neck squamous cell carcinoma progression by transcriptionally activating NR4A1显示文摘SAM pointed domain containing E26 transformation-specific transcription factor(SPDEF)plays dual roles in the initiation and development of human malignancies.However,the biological role of SPDEF in head and neck squamous cell carcinoma(HNSCC)remains unclear.In this study,the expression level of SPDEF and its correlation with the clinical parameters of patients with HNSCC were determined using TCGA-HNSC,GSE65858,and our own clinical cohorts.CCK8,colony formation,cell cycle analysis,and a xenograft tumor growth model were used to determine the molecular functions of SPDEF in HNSCC.ChIP-qPCR,dual luciferase reporter assay,and rescue experiments were conducted to explore the potential molecular mechanism of SPDEF in HNSCC.Compared with normal epithelial tissues,SPDEF was significantly downregulated in HNSCC tissues.Patients with HNSCC with low SPDEF mRNA levels exhibited poor clinical outcomes.Restoring SPDEF inhibited HNSCC cell viability and colony formation and induced G0/G1 cell cycle arrest,while silencing SPDEF promoted cell proliferation in vitro.The xenograft tumor growth model showed that tumors with SPDEF overexpression had slower growth rates,smaller volumes,and lower weights.SPDEF could directly bind to the promoter region of NR4A1 and promoted its transcription,inducing the suppression of AKT,MAPK,and NF-κB signaling pathways.Moreover,silencing NR4A1 blocked the suppressive effect of SPDEF in HNSCC cells.Here,we demonstrate that SPDEF acts as a tumor suppressor by transcriptionally activating NR4A1 in HNSCC.Our findings provide novel insights into the molecular mechanism of SPDEF in tumorigenesis and a novel potential therapeutic target for HNSCC. | Yanting Wang Xianyue Ren Weiyu Li Ruoyan Cao Suyang Liu Laibo Jiang Bin Cheng Juan Xia | 2021 | International Journal of Oral Science2021，13，4： | 3 |
| 17 | PINK1 kinase dysfunction triggers neurodegeneration in the primate brain without impacting mitochondrial homeostasis显示文摘In vitro studies have established the prevalent theory that the mitochondrial kinase PINK1 protects neurodegeneration by removing damaged mitochondria in Parkinson's disease(PD).However,difficulty in detecting endogenous PINK1 protein in rodent brains and cell lines has prevented the rigorous investigation of the in vivo role of PINK1.Here we report that PINK1 kinase form is selectively expressed in the human and monkey brains.CRISPR/Cas9-mediated deficiency of PINK1 causes similar neurodegeneration in the brains of fetal and adult monkeys as well as cultured monkey neurons without affecting mitochondrial protein expression and morphology.Importantly,PINK1 mutations in the primate brain and human cells reduce protein phosphorylation that is important for neuronal function and survival.Our findings suggest that PINK1 kinase activity rather than its mitochondrial function is essential for the neuronal survival in the primate brains and that its kinase dysfunction could be involved in the pathogenesis of PD. | Weili Yang Xiangyu Guo Zhuchi Tu Xiusheng Chen Rui Han Yanting Liu Sen Yan Qi Wang Zhifu Wang Xianxian Zhao Yunpeng Zhang Xin Xiong Huiming Yang Peng Yin Huida Wan Xingxing Chen Jifeng Guo Xiao-Xin Yan Lujian Liao Shihua Li Xiao-Jiang Li | 2022 | Protein & Cell2022，13，1： | 3 |
| 18 | Effect of Different Sowing Dates in South Henan 's Rice-growing Areas on the Growth and Yield of Ratoon Rice显示文摘In order to determine the optimal sowing date of ratoon rice in South Henan's rice-growing areas,this paper performs a comparative analysis of rice growth process,seedling quality and yield of first season rice and ratoon rice under different sowing date treatments. The results show that under climatic conditions( 2014),by using dry seedling cultivation in a small plastic shed,the growth of seedling sown on February21 was affected,while the sowing treatments from March 1 to April 11 can breed normal seedlings,and in this period,the maturity period of first season rice was delayed with prolonged sowing date,and ratoon rice yield declined with prolonged sowing date( total production of rice sown on 11 March reaching a peak). Thus,it is considered that the optimal sowing date of ratoon rice in South Henan's rice-growing areas is mid-March. | Xiangchen LIU Daqing FENG Guilong YU Haiying ZHAO Li QIAO Yanting LI Xuejun FAN Mingcheng LIU Qiuju ZHANG | 2016 | Asian Agricultural Research2016，8，2： | 3 |
| 19 | MXene based mechanically and electrically enhanced film for triboelectric nanogenerator显示文摘The development of triboelectric nanogenerator(TENG)technology which can directly convert ambient mechanical energy into electric energy may affect areas from green energy harvesting to emerging wearing electronics.And,the material of triboelectric layer is critical to the mechanical robustness and electrical output characteristics of the TENGs.Herein,a MXene enhanced electret polytetrafluoroethylene(PTFE)film with a high mechanical property and surface charge density is developed.The MXene/PTFE composite film was synthesized by spraying and annealing treatment.With the doping of MXene,the crystallinity of composite film could be tuned,leading to an enhancement in the tensile property of 450%and reducing the wear volume about 80%in the friction test.Furthermore,the as-fabricated TENG with this composite film outputs 397 V of open-circuit voltage,21µA of short-circuit current,and 232 nC of transfer charge quantity,which are 4,6,and 6 times higher than that of the TENG made by pure PTFE film,respectively.Therefore,this work provides a creative strategy to simultaneously improve the mechanical property and electrical performance of the TENGs,which have great potential in improving device stability under a complex mechanical environment. | Yuyu Gao Guoxu Liu Tianzhao Bu Yaoyao Liu Youchao Qi Yanting Xie Shaohang Xu Weili Deng Weiqing Yang Chi Zhang | 2021 | Nano Research2021，14，12： | 2 |
| 20 | Global analysis of lysine succinylation in patchouli plant leaves显示文摘Lysine succinylation is a novel,naturally occurring posttranslational modification(PTM)in living organisms.Global lysine succinylation identification has been performed at the proteomic level in various species;however,the study of lysine succinylation in plant species is relatively limited.Patchouli plant(P.cablin(Blanco)Benth.,Lamiaceae)is a globally important industrial plant and medicinal herb.In the present study,lysine succinylome analysis was carried out in patchouli plants to determine the potential regulatory role of lysine succinylation in patchouli growth,development,and physiology.The global succinylation sites and proteins in patchouli plants were screened with an immunoprecipitation affinity enrichment technique and advanced mass spectrometry-based proteomics.Several bioinformatic analyses,such as function classification and enrichment,subcellular location predication,metabolic pathway enrichment and protein−protein interaction networking,were conducted to characterize the functions of the identified sites and proteins.In total,1097 succinylation sites in 493 proteins were detected in patchouli plants,among which 466 succinylation sites in 241 proteins were repeatedly identified within three independent experiments.The functional characterization of these proteins indicated that the tricarboxylic acid(TCA)cycle,oxidative phosphorylation,photosynthesis processes,and amino acid biosynthesis may be regulated by lysine succinylation.In addition,these succinylated proteins showed a wide subcellular location distribution,although the chloroplast and cytoplasm were the top two preferred cellular components.Our study suggested the important role of lysine succinylation in patchouli plant physiology and biology and could serve as a useful reference for succinylation studies in other medicinal plants. | Xiaobing Wang Xiuzhen Chen Junren Li Xuanxuan Zhou Yanting Liu Liting Zhong Yun Tang Hai Zheng Jiyun Liu Ruoting Zhan Likai Chen | 2019 | Horticulture Research2019，6，1： | 2 |
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