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| 1 | Temperature pattern dynamics in shocked porous materials显示文摘The physical fields in porous materials under strong shock wave reaction are very complicated. We simulate such systems using the grain contact material point method. The complex temperature fields in the material are treated with the morphological characterization. To compare the structures and evolution of characteristic regimes under various temperature thresholds, we introduce two concepts, structure similarity and process similarity. It is found that the temperature pattern dynamics may show high similarity under various conditions. Within the same material, the structures and evolution of high-temperature regimes may show high similarity if the shock strength and temperature threshold are chosen appropriately. For process similarity in materials with high porosity, the required temperature threshold increases parabolically with the impact velocity. When the porosity becomes lower, the increasing rate becomes higher. For process similarity in different materials, the required temperature threshold and the porosity follow a power-law relationship in some range. | XU AiGuo*, ZHANG GuangCai, LI Hua, YING YangJun, YU XiJun & ZHU JianShi National Key Laboratory of Computational Physics, Institute of Applied Physics and Computational Mathematics, Beijing 100088, China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，8： | 7 |
| 2 | A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Selection显示文摘Chinese cabbage is the most consumed leafy crop in East Asian countries.However,premature bolting induced by continuous low temperatures severely decreases the yield and quality of the Chinese cabbage, and therefore restricts its planting season and geographic distribution.In the past 40years,spring Chinese cabbage with strong winterness has been selected to meet the market demand.Here,we report a genome variation map of Chinese cabbage generated from the resequencing data of 194 geographically diverse accessions of three ecotypes.In-depth analyses of the selection sweeps and genome-wide patterns revealed that spring Chinese cabbage was selected from a specific population of autumn Chinese cabbage around the area of Shandong peninsula in northern China.We identified 23 genomic loci that underwent intensive selection,and further demonstrated by gene expression and haplotype analyses that the incorporation of elite alleles of VERNALISATION INSENTIVE 3.1(BrVIN3.1)and FLOWER LOCUS C 1(BrFLC1)is a determinant genetic source of variation during selection.Moreover,we showed that the quantitative response of BrVIN3.1 to cold due to the sequence variations in the cis elements of the BrVlN3.1 promoter significantly contributes to bolting-time variation in Chinese cabbage.Collectively, our study provides valuable insights into the genetic basis of spring Chinese cabbage selection and will facilitate the breeding of bolting-resistant Varieties by molecular-marker-assisted selection,transgenic or gene editingapproaches. | Tongbing Su Weihong Wang Peirong Li Bin Zhang Pan Li Xiaoyun Xin Honghe Sun Yangjun Yu Deshuang Zhang Xiuyun Zhao Changlong Wen Gang Zhou Yuntong Wang Hongkun Zheng Shuancang Yu Fenglan Zhang | 2018 | Molecular Plant2018，11，11： | 6 |
| 3 | Identification of long noncoding RNAs involved in resistance to downy mildew in Chinese cabbage显示文摘Brassica downy mildew,a severe disease caused by Hyaloperonospora brassicae,can cause enormous economic losses in Chinese cabbage(Brassica rapa L.ssp.pekinensis)production.Although some research has been reported recently concerning the underlying resistance to this disease,no studies have identified or characterized long noncoding RNAs involved in this defense response.In this study,using high-throughput RNA sequencing,we analyzed the disease-responding mRNAs and long noncoding RNAs in two resistant lines(T12–19 and 12–85)and one susceptible line(91–112).Clustering and Gene Ontology analysis of differentially expressed genes(DEGs)showed that more DEGs were involved in the defense response in the two resistant lines than in the susceptible line.Different expression patterns and proposed functions of differentially expressed long noncoding RNAs among T12–19,12–85,and 91–112 indicated that each has a distinct disease response mechanism.There were significantly more cis-and trans-functional long noncoding RNAs in the resistant lines than in the susceptible line,and the genes regulated by these RNAs mostly participated in the disease defense response.Furthermore,we identified a candidate resistance-related long noncoding RNA,MSTRG.19915,which is a long noncoding natural antisense transcript of a MAPK gene,BrMAPK15.Via an agroinfiltration-mediated transient overexpression system and virus-induced gene silencing technology,BrMAPK15 was indicated to have a greater ability to defend against pathogens.MSTRG.19915-silenced seedlings showed enhanced resistance to downy mildew,probably because of the upregulated expression of BrMAPK15.This research identified and characterized long noncoding RNAs involved in resistance to downy mildew,laying a foundation for future in-depth studies of disease resistance mechanisms in Chinese cabbage. | Bin Zhang Tongbing Su Peirong Li Xiaoyun Xin Yunyun Cao Weihong Wang Xiuyun Zhao Deshuang Zhang Yangjun Yu Dayong Li Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 4 | Genome-wide analysis of changes in miRNA and target gene expression reveals key roles in heterosis for chinese cabbage biomass显示文摘Heterosis is a complex phenomenon in which hybrids show better phenotypic characteristics than their parents do.Chinese cabbage(Brassica rapa L.spp.pekinensis)is a popular leafy crop species,hybrids of which are widely used in commercial production;however,the molecular basis of heterosis for biomass of Chinese cabbage is poorly understood.We characterized heterosis in a Chinese cabbage hybrid cultivar and its parental lines from the seedling stage to the heading stage;marked heterosis of leaf weight and biomass yield were observed.Small RNA sequencing revealed 63 and 50 differentially expressed microRNAs(DEMs)at the seedling and early-heading stages,respectively.The expression levels ofthe majority of miRNA clusters in the hybrid were lower than the mid-parent values(MPVs).Using degradome sequencing,we identi fied 1,819 miRNA target genes.Gene ontology(GO)analyses demonstrated that the target genes ofthe MPV-DEMs and low parental expression level dominance(ELD)miRNAs were signi ficantly enriched in leaf morphogenesis,leaf development,and leaf shaping.Transcriptome analysis revealed that the expression levels of photosynthesis and chlorophyll synthesis-related MPV-DEGs(differentially expressed genes)were signi ficantly different in the F_(1) hybrid compared to the parental lines,resulting in increased photosynthesis capacity and chlorophyll content in the former.Furthermore,expression of genes known to regulate leaf development was also observed at the seedling stage.Arabidopsis plants overexpressing BrGRF4.2 and bra-miR396 presented increased and decreased leaf sizes,respectively.These results provide new insight into the regulation of target genes and miRNA expression patterns in leaf size and heterosis for biomass of B.rapa. | Peirong Li Tongbing Su Deshuang Zhang Weihong Wang Xiaoyun Xin Yangjun Yu Xiuyun Zhao Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 1 |
| 5 | Junction plakoglobin regulates and destabilizes HIF2α to inhibit tumorigenesis of renal cell carcinoma显示文摘Background:Increased hypoxia-inducible factor 2α(HIF2α)activation is a common event in clear cell renal cell carcinoma(ccRCC)progression.However,the function and underlying mechanism of HIF2α in ccRCC remains uninvestigated.We conducted this study to access the potential link between junction plakoglobin(JUP)and HIF2αin ccRCC.Methods:Affinity purification and mass spectrometry(AP-MS)screening,glutathione-s-transferase(GST)pull-down and co-immunoprecipitation(Co-IP)assays were performed to detect the interacting proteins of HIF2α.Quantitative PCR(qPCR)and Western blotting were used to detect the expression of JUP in human ccRCC samples.Luciferase reporter assays,chromatin immunoprecipitation(ChIP),cycloheximide chase assays,and ubiquitination assays were conducted to explore the regulation of JUP on the activity of HIF2α.Cell Counting Kit-8(CCK-8)assays,colony formation assays,transwell assays,and xenograft tumor assays were performed to investigate the effect of JUP knockdown or overexpression on the tumorigenicity of renal cancer cells.Results:We identified JUP as a novel HIF2α-binding partner and revealed an important role of JUP in recruiting von Hippel-Lindau(VHL)and histone deacetylases 1/2(HDAC1/2)to HIF2α to regulate its stability and transactivation.JUP knockdown promoted and overexpression suppressed the tumorigenicity of renal cell carcinoma in vitro and in vivo.Importantly,the low expression of JUP was found in clinical ccRCC samples and correlated with enhanced hypoxia scores and poor treatment outcomes.Conclusion:Taken together,these data support a role of JUP in modulating HIF2α signaling during ccRCC progression and identify JUP as a potential therapeutic target. | Ke Chen Jin Zeng Yi Sun Wei Ouyang Gan Yu Hui Zhou Yangjun Zhang Weimin Yao Wei Xiao Junhui Hu Jinchun Xing Kefeng Xiao Lily Wu Zhiqiang Chen Zhangqun Ye Hua Xu | 2021 | Cancer Communications2021，41，4： | 1 |
| 6 | Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis)显示文摘 | Shuancang Yu Fenglan Zhang Renbo Yu Yanmin Zou Jiani Qi Xiuyun Zhao Yangjun Yu Deshuang Zhang Li Li | 2009 | Molecular Breeding2009，，4： | 1 |
| 7 | Biomimetic hybrid hydrogel for hemostasis,adhesion prevention and promoting regeneration after partial liver resection显示文摘Partial liver resection is an established treatment for hepatic disorders.However,surgical bleeding,intra-abdominal adhesion and rapid liver regeneration are still major challenges after partial liver resection,associated with morbidity and mortality.Herein,a biomimetic hybrid hydrogel,composed of oxidized hyaluronic acid,glycol chitosan and MenSCs-derived conditioned medium(CM),is presented to address these issues.The hybrid hydrogel is formed through reversible Schiff base,and possesses injectability and self-healing capability.Moreover,hybrid hydrogel exhibits the capabilities of hemostasis,anti-infection,tissue adhesion and controllable release of cargoes.Based on in vivo studies of the multifunctional hybrid hydrogel,it is demonstrated that acute bleeding in partial liver resection can be ceased immediately by virtue of the hemostasis features of hybrid hydrogel.Also,a significant reduction of intra-abdominal adhesion is confirmed in hybrid hydrogel-treated resection surface.Furthermore,upon the treatment of hybrid hydrogel,hepatic cell proliferation and tissue regeneration can be significantly improved due to the controllably released cytokines from MenSCs-derived CM,exerting the effects of mitogenesis and anti-inflammation in vivo.Thus,the biomimetic hybrid hydrogel can be a promising candidate with great potential for application in partial liver resection. | Zuhong Li Yalei Zhao Xiaoxi Ouyang Ya Yang Yangjun Chen Qixia Luo Yanhong Zhang Danhua Zhu Xiaopeng Yu Lanjuan Li | 2022 | Bioactive Materials2022，7，5： | 0 |
| 8 | 离子在等离子体中的电荷态分布与尾场调制效应研究显示文摘Plasma,called the fourth form of matter,not only plays an important role in astrophysics,but also becomes the new interest in atomic physics,material science and other aspects.Ion beam–plasma interaction can be a new solution to understand the plasma properties.For instance the collision of ion and free electrons dominate in plasma,which can induce the higher energy loss and the change of charge state,as well as the wake-field can modify the ion beam from a continues mode to a pulsed mode with a focused/unfocused spot. | Cheng Rui Wang Yuyu Zhou Xianming Lei Yu Chen Yanhong Zhao Yongtao He Bin Wu Dong Ying Yangjun Ren Jieru Gao Fei Hu Zhanghu Ma Xinwen Xiao Guoqing | 2018 | IMP & HIRFL Annual Report2018，，1： | 0 |
| 9 | A new photolabeling probe for efficient enrichment and deep profiling of cell surface membrane proteome by mass spectrometry显示文摘The cell surface membrane proteome is a class of proteins encoded by ~25% of all protein-coding genes in living organisms and plays a key role in mediating communication between the cells and their surrounding environment. However, most cell surface membrane proteins(CSMPs) are naturally expressed at very low levels compared with intracellular proteins. The difficulties in their purification with high specificity further hinder the understanding of their structure and function. In this study, we developed a new photolabeling probe to achieve efficient tagging and facile enrichment of the CSMPs. The probe is composed of a lipid tail for cell surface localization, a polyethylene glycol(PEG) spacer for increased water solubility, two 4-(N-maleimido)benzophenone(MBP) groups for UV-active tagging of the CSMPs, and a biotin tag for subsequent isolation. Application of this photolabeling probe resulted in the successful enrichment and identification of 3098 annotated CSMPs in HT22 cells with close to 70% selectivity. The proposed photolabeling probe and enrichment strategy were demonstrated to be a powerful method for deep cell surface proteome profiling, representing one of the largest groups of current drug targets. | Yuanyuan Li Chaoshuang Xia Hongxian Zhao Yuping Xie Yangjun Zhang Wanjun Zhang Yongliang Yu Jianhua Wang Weijie Qin | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
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