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| 1 | Response to Selection for Fast Growth in the Second Generation of Pacific Oyster (Crassostrea gigas)显示文摘Mass selection for fast growth was conducted in three Pacific oyster (Crassostrea gigas) stocks from China, Japan and Korea using previously established lines (CS1, JS1, and KS1). To determine whether continuous progress can be achieved by selection for growth, the progeny of three second-generation Pacific oyster lines was selected for shell height and evaluated via a 400-day farming experiment. When harvested at the end of the experiment, the selected crosses of CS2, JS2, and KS2 lines grew by 9.2%, 10.2% and 9.6% larger than the control crosses, respectively. During grow-out stage, the genetic gain of three selected lines was (10.2 ± 1.4)%, (10.4 ± 0.3)%, and (8.4 ± 1.6)%, respectively; and the corresponding realized heritability was 0.457 ± 0.143, 0.312 ± 0.071 and 0.332 ± 0.009, respectively. These results indicated that the selection for fast growth achieved steady progress in the second generation of oyster. Our work provides supportive evidence for the continuity of the Pacific oyster selective breeding program. | WANG Qingzhi LI Qi KONG Lingfeng YU Ruihai | 2012 | Journal of Ocean University of China2012，11，3： | 15 |
| 2 | Kinematics and Dynamics Analysis of a Quadruped Walking Robot with Parallel Leg Mechanism显示文摘It is desired to require a walking robot for the elderly and the disabled to have large capacity,high stiffness,stability,etc.However,the existing walking robots cannot achieve these requirements because of the weight-payload ratio and simple function.Therefore,Improvement of enhancing capacity and functions of the walking robot is an important research issue.According to walking requirements and combining modularization and reconfigurable ideas,a quadruped/biped reconfigurable walking robot with parallel leg mechanism is proposed.The proposed robot can be used for both a biped and a quadruped walking robot.The kinematics and performance analysis of a 3-UPU parallel mechanism which is the basic leg mechanism of a quadruped walking robot are conducted and the structural parameters are optimized.The results show that performance of the walking robot is optimal when the circumradius R,r of the upper and lower platform of leg mechanism are 161.7 mm,57.7 mm,respectively.Based on the optimal results,the kinematics and dynamics of the quadruped walking robot in the static walking mode are derived with the application of parallel mechanism and influence coefficient theory,and the optimal coordination distribution of the dynamic load for the quadruped walking robot with over-determinate inputs is analyzed,which solves dynamic load coupling caused by the branches' constraint of the robot in the walk process.Besides laying a theoretical foundation for development of the prototype,the kinematics and dynamics studies on the quadruped walking robot also boost the theoretical research of the quadruped walking and the practical applications of parallel mechanism. | WANG Hongbo SANG Lingfeng HU Xing ZHANG Dianfan YU Hongnian | 2013 | Chinese Journal of Mechanical Engineering2013，26，5： | 12 |
| 3 | Applications of two-way satellite time and frequency transfer in the BeiDou navigation satellite system显示文摘A two-way satellite time and frequency transfer(TWSTFT) device equipped in the BeiDou navigation satellite system(BDS)can calculate clock error between satellite and ground master clock. TWSTFT is a real-time method with high accuracy because most system errors such as orbital error, station position error, and tropospheric and ionospheric delay error can be eliminated by calculating the two-way pseudorange difference. Another method, the multi-satellite precision orbit determination(MPOD)method, can be applied to estimate satellite clock errors. By comparison with MPOD clock estimations, this paper discusses the applications of the BDS TWSTFT clock observations in satellite clock measurement, satellite clock prediction, navigation system time monitor, and satellite clock performance assessment in orbit. The results show that with TWSTFT clock observations, the accuracy of satellite clock prediction is higher than MPOD. Five continuous weeks of comparisons with three international GNSS Service(IGS) analysis centers(ACs) show that the reference time difference between BeiDou time(BDT) and golbal positoning system(GPS) time(GPST) realized IGS ACs is in the tens of nanoseconds. Applying the TWSTFT clock error observations may obtain more accurate satellite clock performance evaluation in the 104 s interval because the accuracy of the MPOD clock estimation is not sufficiently high. By comparing the BDS and GPS satellite clock performance, we found that the BDS clock stability at the 103 s interval is approximately 10.12, which is similar to the GPS IIR. | ShanShi Zhou XiaoGong Hu Li Liu Rui Guo LingFeng Zhu ZhiQiao Chang ChengPan Tang XiuQiang Gong Ran Li Yang Yu | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，10： | 6 |
| 4 | Flexible, transparent nanocellulose paper-based perovskite solar cells显示文摘Recently perovskite solar cells(PSCs),as photoelectric conversion devices,exhibit excellent power conversion efficiency(PCE)and low-processing cost,and have become one of the most promising devices to replace conventional silicon-based solar cells and address current pressing energy issues.Among them,the flexible PSCs are especially more widely applicable and may propel the rapid advancements of wearable electronics,causing a significant paradigm shift in consumer electronics.Current flexible PSCs use non-biodegradable petroleum-based polymer substrates,discarding of which will aggravate“white pollution”.Therefore,development of green,biodegradable and low-cost flexible substrates will provide a great alternative to flexible PSCs.Here we have developed transparent nanocellulose paper(NCP)with coating of acrylic resin as substrates to fabricate flexible PSCs,which are biodegradable and easily disposable.The PCE of these NCP-based PSCs reached 4.25%,while the power per weight(the ratio of power to device weight)was as high as 0.56 W g^(–1).The flexible PSCs also showed good stability,retaining>80%of original efficiency after 50 times of bending.The NCP-based substrates can also be applied to other electronic systems,which may prosper next-generation green flexible electronics. | Lei Gao Lingfeng Chao Meihui Hou Jin Liang Yonghua Chen Hai-Dong Yu Wei Huang | 2019 | npj Flexible Electronics2019，3，1： | 5 |
| 5 | A unified level set framework utilizing parameter priors for medical image segmentation显示文摘Image segmentation plays an important role in many medical imaging systems,yet in complex circumstances it remains an open problem.One of the main difculties is the intensity inhomogeneity in an image.In order to tackle this problem,we frst introduce a region-based level set segmentation framework to unify the traditional global and local methods.We then propose two novel parameter priors,i.e.,the local order regularization and interactive regularization,and then utilize them as the constraints of the objective energy function.The objective energy function is fnally minimized via a level set evolution process to achieve image segmentation.Extensive experiments show that the proposed approach has gained signifcant improvements in both accuracy and efciency over the state-of-the-art methods. | WANG LingFeng YU ZeYun PAN ChunHong | 2013 | Science China(Information Sciences)2013，56，11： | 4 |
| 6 | Biochemical Composition and Nutritional Value of Different Shell Color Strains of Pacific Oyster Crassostrea gigas显示文摘The shell color of Pacific oyster(Crassostrea gigas) is a desirable trait; but the nutritional studies on C. gigas with different shell colors have not been conducted. Through successive selective breeding, five shell color strains of black(B), purple(P), orange(O), golden(G) and white(W) C. gigas have been developed. The aim of this study was to evaluate the chemical composition and nutritional value of five shell color strains and one commercial population with a common color. The biochemical composition including moisture, total protein, glycogen, ash, total fat, fatty acids(FA), amino acids and minerals was detected. The results indicated that the protein(50.76%–56.57%) was the major component. The content of glycogen showed a significant difference between orange shell and golden shell strains, as well as between commercial population and golden shell strain. In addition, all shell color strains contained a large amount of essential amino acids(12.20–14.15 g(100 g)^(-1)), of them leucine(2.81–3.29 g(100 g)^(-1)) and lysine(2.79–3.28 g(100 g)^(-1)) were predominant. The oysters were rich in polyunsaturated fatty acids(42.26%–45.24% of total fatty acid) with high levels of DHA(18.53%–21.16% of total fatty acid) and EPA(17.23%–18.68% of total fatty acid). Significant differences of mineral contents(Mg, Zn, Fe and Cu) were identified among the six populations. These results indicated that C. gigas with different shell colors presented rich nutritional value with high protein, glycogen, essential amino acids and polyunsaturated fatty acids. The biochemical composition obtained in this study is useful for selective breeding of C. gigas with different shell colors. | ZHU Yijing LI Qi YU Hong KONG Lingfeng | 2018 | Journal of Ocean University of China2018，17，4： | 4 |
| 7 | Relationship Between Single Nucleotide Polymorphism of Glycogen Synthase Gene of Pacific Oyster Crassostrea gigas and Its Glycogen Content显示文摘Glycogen is important not only for the energy supplementary of oysters, but also for human consumption. High glycogen content can improve the stress survival of oyster. A key enzyme in glycogenesis is glycogen synthase that is encoded by glycogen synthase gene GYS. In this study, the relationship between single nucleotide polymorphisms(SNPs) in coding regions of Crassostrea gigas GYS(Cg-GYS) and individual glycogen content was investigated with 321 individuals from five full-sib families. Single-strand conformation polymorphism(SSCP) procedure was combined with sequencing to confirm individual SNP genotypes of Cg-GYS. Least-square analysis of variance was performed to assess the relationship of variation in glycogen content of C. gigas with single SNP genotype and SNP haplotype. As a consequence, six SNPs were found in coding regions to be significantly associated with glycogen content(P < 0.01), from which we constructed four main haplotypes due to linkage disequilibrium. Furthermore, the most effective haplotype H2(GAGGAT) had extremely significant relationship with high glycogen content(P < 0.0001). These findings revealed the potential influence of Cg-GYS polymorphism on the glycogen content and provided molecular biological information for the selective breeding of good quality traits of C. gigas. | LIU Siwei LI Qi YU Hong KONG Lingfeng | 2017 | Journal of Ocean University of China2017，16，1： | 3 |
| 8 | The electromechanical features of LiNbO_(3) crystal for potential high temperature piezoelectric applications显示文摘Lithium niobate(LiNbO_(3),LN)crystal is a multi-functional material with favorable piezoelectric,nonlinear optical and electro-optic properties.In this study,the electromechanical properties of the radial extensional(RE)and the thickness extensional(TE)modes of the congruent LN are studied and the temperature dependent behaviors are revealed.The RE mode electromechanical coupling factors(kp)for the Y-and Z-oriented discs are calculated and found to be 3.8%and 24.7%,respectively,which are nearly the same as the experimental results of 3.8%and 25.2%,respectively.The maximum RE and thickness shear(TS)modes electromechanical coupling factors are obtained to be 47.6%and 68.5%for the Yx/25and Yx/167crystal cuts,respectively.The LN crystal possesses good temperature stability of the electromechanical coupling factors(RE and TE modes)from 20℃ to 500℃,where the variations of kp and kt for the Y-oriented discs are<8.0%and<1.8%,respectively. | Feifei Chen Lingfeng Kong Wei Song Chao Jiang Shiwei Tian Fapeng Yu Lifeng Qin Xian Zhao | 2019 | Journal of Materiomics2019，5，1： | 3 |
| 9 | Genetic Variation Assessed with Microsatellites in Mass Selection Lines of the Pacific Oyster (Crassostrea gigas) in China显示文摘Four successive mass selection lines of the Pacific oyster, Crassostrea gigas, selected for faster growth in breeding programs in China were examined at ten polymorphic microsatellite loci to assess the level of allelic diversity and estimate the effective population size. These data were compared with those of their base population. The results showed that the genetic variation of the four generations were maintained at high levels with an average allelic richness of 18.8–20.6, and a mean expected heterozygosity of 0.902–0.921. They were not reduced compared with those of their base population. Estimated effective population sizes based on temporal variances in microsatellite frequencies were smaller to that of sex ratio-corrected broodstock count estimates. Using a relatively large number of broodstock and keeping an equal sex ratio in the broodstock each generation may have contributed to retaining the original genetic diversity and maintaining relatively large effective population size. The results obtained in this study showed that the genetic variation was not affected greatly by mass selection progress and high genetic variation still existed in the mass selection lines, suggesting that there is still potential for increasing the gains in future generations of C. gigas. The present study provided important information for future genetic improvement by selective breeding, and for the design of suitable management guidelines for genetic breeding of C. gigas. | WANG Xubo LI Qi YU Hong KONG Lingfeng | 2016 | Journal of Ocean University of China2016，15，6： | 2 |
| 10 | Linkage Disequilibrium in Wild and Cultured Populations of Pacific Oyster(Crassostrea gigas)显示文摘Linkage disequilibrium(LD) can be applied for mapping the actual genes responsible for variation of economically important traits through association mapping.The feasibility and efficacy of association studies are strongly dependent on the extent of LD which determines the number and density of markers in the studied population,as well as the experimental design for an association analysis.In this study,we first characterized the extent of LD in a wild population and a cultured mass-selected line of Pacific oyster(Crassostrea gigas).A total of 88 wild and 96 cultured individuals were selected to assess the level of genome-wide LD with 53 microsatellites,respectively.For syntenic marker pairs,no significant association was observed in the wild population;however,three significant associations occurred in the cultured population,and the significant LD extended up to 12.7 c M,indicating that strong artificial selection is a key force for substantial increase of genome-wide LD in cultured population.The difference of LD between wild and cultured populations showed that association studies in Pacific oyster can be achieved with reasonable marker densities at a relatively low cost by choosing an association mapping population.Furthermore,the frequent occurrence of LD between non-syntenic loci and rare alleles encourages the joint application of linkage analysis and LD mapping when mapping genes in oyster.The information on the linkage disequilibrium in the cultured population is useful for future association mapping in oyster. | GUO Xiang LI Qi KONG Lingfeng YU Hong | 2016 | Journal of Ocean University of China2016，15，2： | 2 |
| 11 | Seasonal Variation of the Glycogen Enzyme Activity in Diploid and Triploid Pacific Oyster Gonad During Sexual Maturation显示文摘The glycogen content and the activities of two key enzymes in glycogen metabolism, glycogen phosphorylase and gly- cogen synthetase, in the gonad of diploid and triploid Pacific oysters (Crassostrea gigas) were compared during maturation. The glycogen content in the gonad of diploids decreased with gametogenesis (by 85.7%), but the glycogen content in the gonad of trip- loids did not vary significantly. Activity of glycogen phosphorylase (GP) in the gonad of diploids decreased with gametogenesis (by 55.5%), while GP activity of triploids did not vary significantly during maturation. Activity of glycogen synthetase (GS) in the gonad of diploids increased slightly with gametogenesis, reaching a peak in June. Activity of GS declined sharply from June to July, which might be due to gonad spawning. GS activity of triploid oysters in spawning time (July and August) was significantly higher than that in other months, which might be explained with a ‘compensating’ mechanism for the higher glycogen content in triploids. | KONG Lingfeng WANG Zhaoping YU Ruihai LI Qi WANG Rucai | 2007 | Journal of Ocean University of China2007，6，4： | 1 |
| 12 | Synthesis and Characterization of Substitutional and Interstitial Nitrogen - doped Titanium Dioxides with Visible Light Photocata- lytic Activity 显示文摘 | Peng Feng Cai LingFeng Yu Hao | 2008 | Journal of Solid State Chemistry2008，181，1： | 1 |
| 13 | Microsatellite-Centromere Mapping in Japanese Scallop(Patinopecten yessoensis) Through Half-Tetrad Analysis in Gynogenetic Diploid Families显示文摘Gene-centromere mapping is an essential prerequisite for understanding the composition and structure of genomes. Half-tetrad analysis is a powerful tool for mapping genes and understanding chromosomal behavior during meiosis. The Japanese scallop(Patinopecten yessoensis), a cold-tolerant species inhabiting the northwestern Pacific coast, is a commercially important marine bivalve in Asian countries. In this study, inheritance of 32 informative microsatellite loci was examined in 70-h D-shaped larvae of three induced meiogynogenetic diploid families of P. yessoensis for centromere mapping using half-tetrad analysis. The ratio of gynogenetic diploids was proven to be 100%, 100% and 96% in the three families, respectively. Inheritance analysis in the control crosses showed that 51 of the 53 genotypic ratios observed were in accordance with Mendelian expectations at the 5% level after Bonferroni correction. Seven of the 32 microsatellite loci showed the existence of null alleles in control crosses. The second division segregation frequency(y) of the microsatellite loci ranged from 0.07 to 0.85 with a mean of 0.38, suggesting the existence of positive interference after a single chiasma formation in some chromosomes in the scallop. Microsatellite-centromere distances ranged from 4 c M to 42 c M under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards the assembly of genetic maps in the commercially important scallop species. | LI Qi QI Mingjun NIE Hongtao KONG Lingfeng YU Hong | 2016 | Journal of Ocean University of China2016，15，3： | 1 |
| 14 | Development of Three Multiplex PCR Primer Sets for Ark Shell(Scapharca broughtonii)and Their Validation in Parentage Assignment显示文摘Scapharca broughtonii is a commercially important and over-exploited species.In order to investigate its genetic diversity and population structure,43 novel polymorphic microsatellites were isolated and characterized.The number of alleles per locus ranged from 3 to 22 with an average of 6.93,and the observed and expected heterozygosities varied between 0.233 and 1.000,and 0.250 and 0.953,with an average of 0.614 and 0.707,respectively.Three highly informative multiplex PCRs were developed from nine of those microsatellites for S.broughtonii.We evaluated and validated these multiplex PCRs in 8 full-sib families.The average polymorphism information content(PIC) was 0.539.The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns.Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets.Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S.broughtonii.The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction,population genetic analysis and brood stock management. | LINing LI Qi KONG Lingfeng YU Hong | 2016 | Journal of Ocean University of China2016，15，2： | 1 |
| 15 | Phylogenomic resolution of Imparidentia(Mollusca:Bivalvia)diversifcation through mitochondrial genomes显示文摘Despite signifcant advances in the phylogenomics of bivalves over the past decade,the higher-level phylogeny of Imparidentia(a superorder of Heterodonta)remains elusive.Here,a total of fve new mitochondrial sequences(Chama asperella,Chama limbula,Chama dunkeri,Barnea manilensis and Ctena divergens)was added to provide resolution in nodes that required additional study.Although the monophyly of Lucinida remains less clear,the results revealed the overall backbone of the Imparidentia tree and the monophyly of Imparidentia.Likewise,most relationships among the fve major Imparidentia lineages—Lucinida,Cardiida,Adapedonta,Myida and Venerida—were addressed with a well-supported topology.Basal relationships of Imparidentia recovered Lucinidae as the sister group to all remaining imparidentian taxa.Thyasiridae is a sister group to other imparidentian bivalves(except Lucinidae species)which is split into Cardiida,Adapedonta and the divergent clade of Neoheterodontei.Neoheterodontei was comprised of Venerida and Myida,the former of which now also contains Chamidae as the sister group to all the remaining venerid taxa.Moreover,molecular divergence times were inferred by calibrating nine nodes in the Imparidentia tree of life by extinct taxa.The origin of these major clades ranged from Ordovician to Permian with the diversifcation through the Palaeozoic to Mesozoic.Overall,the results obtained in this study demonstrate a better-resolved Imparidentia phylogeny based on mitochondrial genomes. | Yu Wang Yi Yang Lingfeng Kong Takenori Sasaki Qi Li | 2023 | Marine Life Science & Technology2023，5，3： | 1 |
| 16 | Multidimentional data encryption with digital holography显示文摘 | Lingfeng Yu Lilong Cai | 2003 | Optics Communications2003，215，： | 1 |
| 17 | High-resolution Quantitative Phase-contrast Microscopy by Digital Holography 显示文摘 | MANN Christopher J YU Lingfeng LO Chun-Min | 2005 | Optics Express2005，13，22： | 1 |
| 18 | Cloud-Data Envelopment Analysis Method Used for Assessment of Restoration Building Block Schemes显示文摘The selection of an optimized restoration building block(RBB)scheme among all available schemes is one of the most important factors impacting the power system restoration process after a complete or partial blackout.This paper presents a data envelopment analysis(DEA)model used as an empirical method to assess the RBB schemes.An N-level evaluation scale cloud system is built based on cloud theory to transform qualitative I/O indices of DEA model into quantitative values.Through joint utilization of the CCR(Charnes,Cooper and Rhodes)model and the LJK(Li,Jahanshahloo and Khodabakhshi)model,the established Joint-DEA model makes the newly proposed Cloud-DEA method a more feasible and robust method in assessment of RBB schemes. | Yun Zhou Zheng Yan Naihu Li Lingfeng Yu Lingfeng Zhou Lixia Chen | 2015 | CSEE Journal of Power and Energy Systems2015，1，2： | 1 |
| 19 | Crystallization behavior and hydrophobic properties of biodegradable ethyl cellulose-g-poly (3-hydroxybutyrate-co-3-hydroxyvalerate):The influence of the side-chain length and grafting density Biotransformation in organic chemistry显示文摘 | YU Houyong QIN Zongqi WANG Lingfeng ZHOU Zhe | | 0，，04： | 1 |
| 20 | Wavelength-scanning digital interference holography for tomographic three-dimensional imaging by use of the angular spectrum method 显示文摘 | Lingfeng Yu Myung K Kim | 2005 | Opt Lett2005，30，16： | 1 |
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