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| 1 | The Auxin-Regulated Protein ZmAuxRPI Coordinates the Balance between Root Growth and Stalk Rot Disease Resistance in Maize显示文摘To optimize fitness, plants must efficiently allocate their resources between growth and defense. Although phytohormone crosstalk has emerged as a major player in balancing growth and defense, the genetic basis by which plants man age this balance remai ns elusive. We previously ide ntified a quantitative disease . resistance locus, qRfg2, in maize (Zea mays) that protects against the fungal disease Gibberella stalk rot. Here, through map-based cloning, we demonstrate that the causal gene at qRfg2 is ZmAuxRPI, which encodes a plastid stroma-localized auxin-regulated protein. ZmAuxRPI responded quickly to pathogen challenge with a rapid yet transient reduction in expression that led to arrested root growth but enhanced resista nee to Gibberella stalk rot and Fusarium ear rot. ZmAuxRPI was show n to promote the biosynthesis of indole-3-acetic acid (IAA), while suppressing the formation of benzoxazinoid defense compounds. ZmAuxRPI presumably acts as a resource regulator modulating indole-3-glycerol phosphate and/or indole flux at the branch point between the IAA and benzoxazinoid biosynthetic pathways. The concerted interplay between IAA and benzoxazinoids can regulate the growth-defense balance in a timely and efficient manner to optimize plant fitness. | Jianrong Ye Tao Zhong Dongfeng Zhang Chuanyu Ma Lina Wang Lishan Yao Qianqian Zhang Mang Zhu Mingliang Xu | 2019 | Molecular Plant2019，12，3： | 19 |
| 2 | Coinfection with influenza A virus enhances SARS-CoV-2 infectivity显示文摘The upcoming flu season in the Northern Hemisphere merging with the current COVID-19 pandemic raises a potentially severe threat to public health.Through experimental coinfection with influenza A virus(IAV)and either pseudotyped or live SARS-CoV-2 virus,we found that IAV preinfection significantly promoted the infectivity of SARS-CoV-2 in a broad range of cell types.Remarkably,in vivo,increased SARS-CoV-2 viral load and more severe lung damage were observed in mice coinfected with IAV.Moreover,such enhancement of SARS-CoV-2 infectivity was not observed with several other respiratory viruses,likely due to a unique feature of IAV to elevate ACE2 expression.This study illustrates that IAV has a unique ability to aggravate SARS-CoV-2 infection,and thus,prevention of IAV infection is of great significance during the COVID-19 pandemic. | Lei Bai Yongliang Zhao Jiazhen Dong Simeng Liang Ming Guo Xinjin Liu Xin Wang Zhixiang Huang Xiaoyi Sun Zhen Zhang Lianghui Dong Qianyun Liu Yucheng Zheng Danping Niu Min Xiang Kun Song Jiajie Ye Wenchao Zheng Zhidong Tang Mingliang Tang Yu Zhou Chao Shen Li Zhou Yu Chen Huan Yan Ke Lan Ke Xu | 2021 | Cell Research2021，31，4： | 16 |
| 3 | An Atypical Thioredoxin Imparts Early Resistance to Sugarcane Mosaic Virus in Maize显示文摘 | Qingqing Liu Huanhuan Liu Yangqing Gong Yongfu Tao Lu Jiang Weiliang Zuo Qin Yang Jianrong Ye Jinsheng Lai Jianyu Wu Thomas Lubberstedt Mingliang Xu | 2017 | Molecular Plant2017，10，3： | 14 |
| 4 | Elevating H3K27me3 level sensitizes colorectal cancer to oxaliplatin显示文摘Histone methylation is a context-dependent modification that regulates gene expression,and the trimethylation of histone H3 lysine 27(H3K27me3)usually induces gene silencing.Overcoming colorectal cancer(CRC)chemoresistance is currently a huge challenge,but the relationship between H3K27me3 modification and chemoresistance remains largely unclear.Here,we found that H3K27me3 levels positively correlated with the metastasis-free survival of CRC patients and a low H3K27me3 level predicted a poor outcome upon chemotherapeutic drug treatment.Oxaliplatin stimulation significantly induced the expression of H3K27 lysine demethylase 6A/6B(KDM6A/6B),thus decreasing the level of H3K27me3 in CRC cells.Elevation of H3K27me3 level through KDM6A/6B depletion or GSK-J4(a KDM6A/6B inhibitor)treatment significantly enhanced oxaliplatin-induced apoptosis.Conversely,when inhibiting the expression of H3K27me3 by EPZ-6438,an inhibitor of the histone methyltransferase EZH2,the proportion of apoptotic cells remarkably decreased.In addition,the combination of GSK-J4 and oxaliplatin significantly inhibited tumor growth in an oxaliplatin-resistant patient-derived xenograft model.Importantly,we revealed that oxaliplatin treatment dramatically induced NOTCH2 expression,which was caused by downregulation of H3K27me3 level on the NOTCH2 transcription initiation site.Thus,the activated NOTCH signaling promoted the expression of stemness-related genes,which resulted in oxaliplatin resistance.Furthermore,oxaliplatin-induced NOTCH signaling could be interrupted by GSK-J4 treatment.Collectively,our findings suggest that elevating H3K27me3 level can improve drug sensitivity in CRC patients. | Qi Wang Xi Chen Yuhang Jiang Sanhong Liu Hanshao Liu Xiaohua Sun Haohao Zhang Zhi Liu Yu Tao Cuifeng Li Yiming Hu Dandan Liu Deji Ye Yongzhong Liu Mingliang Wang Xiaoren Zhang | 2020 | Journal of Molecular Cell Biology2020，12，2： | 6 |
| 5 | A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum显示文摘Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained. | ZHU Jun, WANG FangJun, DONG XiaoLi, YE MingLiang & ZOU HanFa Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | 2010 | Science China Chemistry2010，53，4： | 3 |
| 6 | Super-resolution imaging and tracking of TGF-β receptor II on living cells显示文摘Single-particle tracking photoactivated localization microscopy(spt PALM) has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-b type II receptor(Tb RII) at high expression level using both traditional single-particle tracking(SPT) and spt PALM.The ligand-induced aggregation of Tb RII oligomers was further indicated by spt PALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, spt PALM outperforms traditional SPT by providing more accurate biophysical information. | Zi Ye Nan Li Libo Zhao Yahong Sun Hefei Ruan Mingliang Zhang Jinghe Yuan Xiaohong Fang | 2016 | Science Bulletin2016，61，8： | 3 |
| 7 | Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction显示文摘Cardiovascular diseases such as myocardial infarction(MI)are among the major causes of death worldwide.Although intramyocardial injection of hydrogels can effectively enhance the ventricular wall,this approach is limited because of its restriction to the poor vascularization in the infarcted myocardium.Here,we reported a new type of hydrogel composed of alginate(ALG)and hyaluronic acid(HA)with lyophilized platelet-rich fibrin(Ly-PRF)for releasing abundant growth factors to realize their respective functions.The results of in vitro studies demonstrated favorable mechanical property and release ability of ALG-HA with Ly-PRF.When injected into the infarcted myocardium,this composite hydrogel preserved heart function and the Ly-PRF within the hydrogel promoted angiogenesis and increased vascular density in both infarcted and border zone,which rescued the ischemic myocardium.These beneficial effects were also accompanied by macrophage polarization and regulation of myocardial fibrosis.Moreover,the autologous origin of Ly-PRF with ALG-HA hydrogel offers myriad advantages including safety profile,easiness to obtain and cost-effectiveness.Overall,this study demonstrated the versatile therapeutic effects of a novel composite hydrogel ALG-HA with Ly-PRF,which optimizes a promising vascularized substitution strategy for improving cardiac function after MI. | Bei Qian Qi Yang Mingliang Wang Shixing Huang Chenyu Jiang Hongpeng Shi Qiang Long Mi Zhou Qiang Zhao Xiaofeng Ye | 2022 | Bioactive Materials2022，7，1： | 2 |
| 8 | Inhibition of the spread of endophytic Sporisorium reilianum renders maize resistance to head smut显示文摘Head smut, caused by the fungal pathogen Sporisorium reilianum, poses a grave threat to maize(Zea mays) production worldwide. Here we report cytological and molecular evidence for maize resistance to head smut. During early stages of root infection, S. reilianum mycelium was capable of penetrating the root epidermis of both resistant(Ji1037) and susceptible(HZ4) inbred lines. S. reilianum hyphae were observed in the root–stem junction at 6 days after inoculation. In an attempt to monitor hyphal spread within the maize plant,a highly specific and sensitive real-time PCR method was established to estimate the hyphal content in infected maize tissues. During the upward growth of endophytic S.reilianum, the extent of hyphal spread was markedly different between Ji1037 and HZ4. Very little or no pathogen was detected in aerial parts of Ji1037, whereas large amounts of pathogen accumulated in aboveground tissues, particularly inflorescences, of HZ4. Thus,maize resistance to S. reilianum was achieved mainly by inhibition of endophytic hyphal growth rather than by prevention of early-root penetration by the pathogen. | Xianrong Zhao Jianrong Ye Lai Wei Nan Zhang Yuexian Xing Weiliang Zuo Qing Chao Guoqing Tan Mingliang Xu | 2015 | The Crop Journal2015，3，2： | 2 |
| 9 | Monolithic stationary phases for liquid chromatography and capillary electrochromatography显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr: A2002，954，12： | 1 |
| 10 | Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by β_2-adrenergic receptor显示文摘Stimulation of G protein-coupled receptors(GPCRs) can lead to the transactivation of the epidermal growth factor receptors(EGFR). The cross-communication between the two signaling pathways regulates several important physiological or pathological processes. However, the molecule mechanism underlying EGFR transactivation remains poorly understood. Here, we aim to study the GPCR-mediated EGFR transactivation process using the single-molecule fluorescence imaging and tracking approach.We found that although EGFR existed as monomers at the plasma membrane of resting cells, they became dimers and thus diffused slower following the activation of β2-adrenergic receptor(β2-AR) by isoproterenol(ISO). We further proved thatβ2-AR-mediated changes of EGFR in stoichiometry and dynamics were mediated by Src kinase. Thus, the observations obtained via the single-molecule imaging and tracking methods shed new insights into the molecular mechanism of EGFR transactivation at single molecule level. | Mingliang Zhang Kangmin He Jimin Wu Nan Li Jinghe Yuan Wei Zhou Zi Ye Zijian Li Han Xiao Zhizhen Lv Youyi Zhang Xiaohong Fang | 2017 | Science China Chemistry2017，60，10： | 1 |
| 11 | Low-bandgap conjugated polymers based on benzodipyrrolidone with reliable unipolar electron mobility exceeding 1 cm^(2) V^(-1)s^(-1)显示文摘The employment of an intrinsic quinoidal building block,benzodipyrrolidone(BDP),on constructing conjugated polymers(PBDP-2F and PBDP-2CN)with high electron mobility and unipolar transport characteristic in polyethylenimine ethoxylated(PEIE)modified organic field-effect transistors(OFETs)is reported.The intrinsic quinoidal characteristic and excellent coplanarity of BDP can lower the lowest unoccupied molecular orbital(LUMO)levels and improve ordered interchain packing of the resulting polymers in solid states,which are favorable for electron-injection and transport.By using PEIE as the interlayer to block the hole injection,unipolar n-type transport characteristics with high electron mobility of 0.58 and 1.01 cm^(2) V^(-1)s^(-1)were achieved by the OFETs based on PBDP-2F and PBDP-2CN,respectively.More importantly,the extracted mobilities are highly reliable with the reliability factor of above 80%.To the best of our knowledge,PBDP-2CN is the very first quinoid-based conjugated polymer with reliable electron mobility exceeding 1 cm^(2) V^(-1)s^(-1).This work represents a significant step in exploring intrinsic quinoidal CPs for application in n-channel OFETs and logic complementary circuits. | Mingqun Yang Tian Du Xuxia Zhao Xuelong Huang Langheng Pan Shuting Pang Haoran Tang Zhongxiang Peng Long Ye Yunfeng Deng Mingliang Sun Chunhui Duan Fei Huang Yong Cao | 2021 | Science China Chemistry2021，64，7： | 1 |
| 12 | Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification显示文摘 | Lianghai Hu Karl-Siegfried Boos Mingliang Ye Hanfa Zou | 2014 | Talanta2014，，： | 1 |
| 13 | Monolithic stationary for liquid chromatography and capillary electrochromatography显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr : A2002，954，12： | 1 |
| 14 | Monoli- thic stationary ph-ases for liquid chromatography and capi- llaryelectrochromatography显示文摘 | Zou Hanfa Huang Xiaodong Ye Mingliang | 2002 | J Chromatogr A2002，954，1： | 1 |
| 15 | Recent development of monolithic stationary phases with emphasis on microscale chromatographic separation显示文摘 | Wu Ren'an Ye Mingliang Zou Hanfan | 2008 | J Chromatography A2008，1184，： | 1 |
| 16 | Monolithic stationary phases for liquid chromatography and capillary electrochromatograghy显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr: A2002，954，： | 1 |
| 17 | Separation of peptides by strong cation-exchange capillary electro chromatography显示文摘 | Mingliang Ye Hanfa Zou Zhen Liu | 2000 | Chromatography A2000，869，： | 1 |
| 18 | 显示文摘 | TIAN Ruijun SUN Junming ZHANG He YE Mingliang XIE Chuanhui DONG Jin HU Jiwei MA Ding BAO Xinhe ZOU Hanfa | 2006 | Electrophoresis2006，27，4： | 1 |
| 19 | In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer显示文摘 | Kai Li Zhen Sun Jianyong Zheng Yuanyuan Lu Yangyang Bian Mingliang Ye Xin Wang Yongzhan Nie Hanfa Zou Daiming Fan | 2013 | Journal of Proteomics2013，，： | 1 |
| 20 | Monolithic stationary phases for liquid chromatography and capillary electrochromatography显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr: A2002，954，12： | 1 |
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