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| 1 | A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum显示文摘Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained. | ZHU Jun, WANG FangJun, DONG XiaoLi, YE MingLiang & ZOU HanFa Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | 2010 | Science China Chemistry2010，53，4： | 3 |
| 2 | Monolithic stationary phases for liquid chromatography and capillary electrochromatography显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr: A2002，954，12： | 1 |
| 3 | Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping显示文摘 | Honghai Jiang Hanfa Zou Hailin Wang Qiang Zhang Jianyi Ni Qingchun Zhang Zhong Guo Xiaoming Chen | 2000 | Science in China Series B: Chemistry2000，，6： | 1 |
| 4 | A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry显示文摘 | Guo Zhong Zhang Qingchun Zou Hanfa | 2002 | Anal Chem2002，74，7： | 1 |
| 5 | Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification显示文摘 | Lianghai Hu Karl-Siegfried Boos Mingliang Ye Hanfa Zou | 2014 | Talanta2014，，： | 1 |
| 6 | Monolithic stationary for liquid chromatography and capillary electrochromatography显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr : A2002，954，12： | 1 |
| 7 | Short columns with molecularly imprinted monolithic stationary phases for rapid separation of diastereomers and enantiomers显示文摘 | Xiaodong Huang Feng Qin Xiaoming Chen Yueqi Liu Hanfa Zou | 2004 | Journal of Chromatography B2004，，1： | 1 |
| 8 | Determination of arylamine compounds in waste water using solid-phase extraction arid reversed-phase high performance liquid chromatography 显示文摘 | Zhao Shuli Wei Fusheng Zou Hanfa | 1998 | Chemosphere1998，36，： | 1 |
| 9 | Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy显示文摘在 apoptosis 的内在的小径, mitochondrial 强调反应的氧种类(mitoROS ) 可能在 cytosol 比那些作为更有效的信号被察觉到,因为线粒体在细胞 apoptosis 期间是反应的氧种类(ROS ) 和枢轴的部件的主要来源。Mitochondrial superoxide dismutase (SOD2 ) 带头在消除 mitoROS 的角色,和 SOD2 的抑制可能导致严重骚乱压到优势 mitochondrial 氧化平衡,它将提高细胞内部的氧化压力和开车房间到死亡。此处,我们报导一般策略用 2-methoxyestradiol 由 SOD2 的指向的抑制杀死癌症房间(2-ME,为 SOD 家庭的一个禁止者) 经由柔韧的指向线粒体的 mesoporous 硅石 nanocarrier (mtMSN ) ,与 mitoROS 的期望的举起和在 HeLa 房间的 apoptosis 的激活。Fe < 潜水艇 class= “ a-plus-plus ” > 3 O < 潜水艇 class= “ a-plus-plus ” > 4 @MSN 在指向线粒体的药交货和 mitochondrial 酶的选择抑制被采用,并且被显示与好 biocompatibility 和高装载能力稳定。由于由 2-ME/mtMSN 的 SOD2 的选择抑制,提高了 mitoROS 的举起(那中的 132% 个与免费 2-ME ) 被获得,在开始房间 apoptosis 结合了更高的效率(那中的 395% 个与在 4 h 的免费 2-ME ) 。最后, 2-ME/mtMSN 在由利用生物识别并且磁性的指导 HeLa 房间杀死指向展出了强大的功效,引起 97.0% 房间有 2 仅仅 g/mL 2-ME/mtMSN 的死亡,在它在通过精细的 mitochondrial 的操作的癌症治疗的大潜力提示氧化平衡。 | Yi Zhang Zhengyan Hu Guiju Xu Chuanzhou Gao Ren'an Wu Hanfa Zou | 2014 | Nano Research2014，7，8： | 1 |
| 10 | Monoli- thic stationary ph-ases for liquid chromatography and capi- llaryelectrochromatography显示文摘 | Zou Hanfa Huang Xiaodong Ye Mingliang | 2002 | J Chromatogr A2002，954，1： | 1 |
| 11 | Synthesis of a silica-bonded bovine serum albumin albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers显示文摘 | Zhang Qiang Zou Hanfa Wang Hailin | 2000 | J Chromatogr A2000，866，2： | 1 |
| 12 | Monolithic stationary phases for liquid chromatography and capillary electrochromatograghy显示文摘 | ZOU Hanfa HUANG Xiaodong YE Mingliang | 2002 | J Chromatogr: A2002，954，： | 1 |
| 13 | Immobilized metal affinity composite membrane medium for biomoleculars separation显示文摘 | JIA Lingyun ZOU Hanfa | 1998 | Sci in China: B1998，28，6： | 1 |
| 14 | Separation of peptides by strong cation-exchange capillary electro chromatography显示文摘 | Mingliang Ye Hanfa Zou Zhen Liu | 2000 | Chromatography A2000，869，： | 1 |
| 15 | 显示文摘 | TIAN Ruijun SUN Junming ZHANG He YE Mingliang XIE Chuanhui DONG Jin HU Jiwei MA Ding BAO Xinhe ZOU Hanfa | 2006 | Electrophoresis2006，27，4： | 1 |
| 16 | Monolithic stationary phases for liquid chromatography and capillary electrochromatography显示文摘 | Zou Hanfa | 2002 | Journal of Chromatography A2002，45，： | 1 |
| 17 | Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly (glycidyl methacrylate-co-ethylene dimethacrylate) 显示文摘 | Luo Quanzhou Zou Hanfa Xiao Xiangzhu | 2001 | Journal of Chromatography A2001，926，2： | 1 |
| 18 | Preparation and application of organic-silica hybrid monolithic capillary columns显示文摘 | Minghuo Wu Ren' an Wu Zhenbin Zhang Hanfa Zou | 2011 | Electrophoresis2011，32，1： | 1 |
| 19 | Membrane affinity chromatography for analysis and purification of biopolymers显示文摘 | Dongmei Zhou Hanfa Zou Jianyi Ni Hailin Wang Li Yang Yukui Zhang | 1999 | Chromatographia (-)1999，，1： | 1 |
| 20 | In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer显示文摘 | Kai Li Zhen Sun Jianyong Zheng Yuanyuan Lu Yangyang Bian Mingliang Ye Xin Wang Yongzhan Nie Hanfa Zou Daiming Fan | 2013 | Journal of Proteomics2013，，： | 1 |
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