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| 1 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 2 | LncRNA-HOTAIR inhibition aggravates oxidative stress-induced H9c2 cells injury through suppression of MMP2 by miR-125显示文摘 | Linlin Li Mengna Zhang Weizhen Chen Ruirui Wang Zi Ye Yanyan Wang Xiao Li Cheguo Cai | 2018 | Acta Biochimica et Biophysica Sinica2018，50，10： | 20 |
| 3 | Spatial structure optimization of mountainous abandoned mine land reuse based on system dynamics model and CLUE-S model显示文摘The mountainous abandoned mine land is often distributed in the fomi of fragmented patches. Therefore, it can greatly promote the reuse value of abandoned mine land and relieve the pressure of land demand to realize the rational reuse of abandoned mine land based on the future land use structure and spatial layout of mountainous area. In this paper, optimization of the spatial structure of mountainous abandoned mine land reuse is realized through the system dynamics model and CLUE-S model. Mentougou district, Beijing, China is selected as the research area. System dynamics model with feedback functions is constructed to simulate land use structure from 2011 to 2025, which is taken as the quanfiiative constraint on spatial structure optimization. CLUE-S model with neighborhood analysis function is applied to simulate future land use spatial structure. The simulation result layer is superimposed with the abandoned mine land distribution layer and the optimized spatial structure of abandoned mine land reuse then is determined, checked by reuse suitability evaluation. The result shows that abandoned mine land can be fully optimized as other land use types according to demand, and the reuse directions are water conservancy facilities land, urban land, rural residential land, tourism land, garden land, woodland and grassland. The trend of abandoned mine land reuse tend to be consistent with land use types of neighboring patches. This study can provide theoretical reference for the practices of mountainous abandoned mine land reuse. | Linlin Cheng Haiyuan Sun Ye Zhang Shaofeng Zhen | 2019 | International Journal of Coal Science & Technology2019，6，1： | 5 |
| 4 | Quantification of microcystin-producing and non-microcystin producing Microcystis populations during the 2009 and 2010 blooms in Lake Taihu using quantitative real-time PCR显示文摘Lake Taihu,a large,shallow hypertrophic freshwater lake in eastern China,has experienced lake-wide toxic cyanobacterial blooms annually during summer season in the past decades.Spatial changes in the abundance of hepatotoxin microcystin-producing and nonmicrocystin producing Microcystis populations were investigated in the lake in August of 2009 and 2010.To monitor the densities of the total Microcystis population and the potential microcystin-producing subpopulation,we used a quantitative real-time PCR assay targeting the phycocyanin intergenic spacer(PC-IGS) and the microcystin synthetase gene(mcyD),respectively.On the basis of quantification by real-time PCR analysis,the abundance of potential toxic Microcystis genotypes and the ratio of the mcyD subpopulation to the total Microcystis varied significantly,from 4.08×104 to 5.22×107 copies/mL,from 5.7% to 65.8%,respectively.Correlation analysis showed a strong positive relationship between chlorophyll-a,toxic Microcystis and total Microcystis;the abundance of toxic Microcystis correlated positively with total phosphorus and ortho-phosphate concentrations,but negatively with TN:TP ratio and nitrate concentrations.Meanwhile the proportion of potential toxic genotypes within Microcystis population showed positive correlation with total phosphorus and ortho-phosphate concentrations.Our data suggest that increased phosphorus loading may be a significant factor promoting the occurrence of toxic Microcystis bloom in Lake Taihu. | Daming Li Fanxiang Kong Xiaoli Shi Linlin Ye Yang Yu Zhen Yang | 2012 | Journal of Environmental Sciences2012，24，2： | 5 |
| 5 | Development and characterization of a clinical strain of Coxsackievirus A16 and an eGFP infectious clone显示文摘Coxsackievirus A16(CA16) is one of the major causes of hand, foot, and mouth disease(HFMD) worldwide, which is a common illness that affects children. The frequent occurrence of HFMD outbreaks has become a serious public health problem in Asia. Therefore, it is important to understand the pathogenesis and replication of CA16. In this study, a stable infectious c DNA clone of an epidemic strain of Coxsackievirus A16(CA16) was assembled, and subsequently a reporter virus(e GFP-CA16) was constructed by inserting the e GFP gene between the 5'-UTR and the N-terminus of VP4, with the addition of a 2A protease cleavage site(ITTLG) at its C-terminus. This was transfected into Vero cells to generate infectious recombinant viruses. The growth characteristics and plaque morphology, in vitro, in mammalian cells were found to be indistinguishable between the parental and recombinant viruses. Although the e GFP-CA16 showed smaller plaque size as compared to recombinant CA16, both were found to exhibit similar growth trends and EC50 of NITD008. In summary, this stable infectious c DNA clone should provide a valuable experimental system to study CA16 infection and host response. The e GFP-CA16 is expected to provide a powerful tool to monitor e GFP expression in infected cells and to evaluate the antiviral activity of potential antiviral agents in the treatment of CA16 infections. | Chenglin Deng Xiaodan Li Siqing Liu Linlin Xu Hanqing Ye Cheng-Feng Qin Bo Zhang | 2015 | Virologica Sinica2015，30，4： | 5 |
| 6 | Seismic behavior of breakwaters on complex ground by numerical tests:Liquefaction and post liquefaction ground settlements显示文摘A large number of breakwaters have been constructed along coasts to protect humans and infrastructures from tsunamis.There is a risk that foundation soils of these structures may liquefy,or partially liquefy during the earthquake preceding a tsunami,which would greatly reduce the structures’capacity to resist the tsunami.It is necessary to consider not only the soil’s liquefaction behavior due to earthquake motions but also its post-liquefaction behavior because this behavior will affect the breakwater’s capacity to resist an incoming tsunami.In this study,numerical tests based on a sophisticated constitutive model and a soil-water coupled finite element method are used to predict the mechanical behavior of breakwaters and the surrounding soils.Two real breakwaters subjected to two different seismic excitations are examined through numerical simulation.The simulation results show that,earthquakes affect not only the immediate behavior of breakwaters and the surrounding soils but also their long-term settlements due to post-earthquake consolidation.A soil profile with thick clayey layers beneath liquefied soil is more vulnerable to tsunami than a soil profile with only sandy layers.Therefore,quantitatively evaluating the seismic behavior of breakwaters and surrounding soils is important for the design of breakwater structures to resist tsunamis. | Gu Linlin Zhang Feng Bao Xiaohua Shi Zhenming Ye Guanlin Ling Xianzhang | 2018 | Earthquake Engineering and Engineering Vibration2018，17，2： | 3 |
| 7 | NLRP3 Deficiency Attenuates Secondary Degeneration of Visual Cortical Neurons Following Optic Nerve Injury显示文摘In the visual pathway, optic nerve(ON) injury may cause secondary degeneration of neurons in distal regions, such as the visual cortex. However, the role of the neuroinflammatory response in regulating secondary impairment in the visual cortex after ON injury remains unclear. The NOD-like receptor family pyrin domain containing 3(NLRP3) is an important regulator of neuroinflammation. In this study, we established a mouse model of unilateral ON crush(ONC) and showed that the expression of NLRP3 was significantly increased in the primary visual cortex(V1) as a response to ONC and that the NLRP3 inflammasome was activated in the contralateral V1 1 days–14 days after ONC. Ablation of the NLRP3 gene significantly decreased the trans-neuronal degeneration within 14 days. Visual electrophysiological function was improved in NLRP3-/- mice. Taken together, these findings suggest that NLRP3 is a potential therapeutic target for protecting visual cortical neurons against degeneration after ON injury. | Zhou Zhang Wenyi Liu Yubin Huang Linlin Luo Xiaofeng Cai Yunjia Liu Liqianyu Ai Jun Yan Sen Lin Jian Ye | 2020 | Neuroscience Bulletin2020，36，3： | 3 |
| 8 | Changes in dendritic cells and dendritic cell subpopulations in peripheral blood of recipients during acute rejection after kidney transplantation显示文摘 | Ma Linlin Liu Yong Wu Junjie Xu Xiuhong Liu Fen Feng Lang Xie Zelin Tang Yawang Sun Wen Guo Hongbo Zhang Lei Lin Jun Tian Ye | 2014 | Chinese Medical Journal2014，，8： | 3 |
| 9 | Postzygotic single-nucleotide mosaicisms in whole-genome sequences of clinically unremarkable individuals显示文摘 | August Y Huang Xiaojing Xu Adam Y Ye Qixi Wu Linlin Yan Boxun Zhao Xiaoxu Yang Yao He Sheng Wang Zheng Zhang Bowen Gu Han-Qing Zhao Meng Wang Hua Gao Ge Gao Zhichao Zhang Xiaoling Yang Xiru Wu Yuehua Zhang Liping Wei | 2014 | Cell Research2014，24，11： | 2 |
| 10 | Characterization of oogonia stem cells in mice by Fragilis显示文摘Dear Editor,Identification of oogonia stem cells would have great potentials in infertility treatment and fertility preservation.Here we tested whether Fragilis/lfitm3 can identify oogonia stem cells in fetal mouse ovaries and their molecular features,if it can,and whether the oogonia stem cells marked by Fragilis can be found in the postnatal ovaries. | Xiaoyan Sheng Chenglei Tian Linlin Liu Lingling Wang Xiaoying Ye Jie Li Ming Zeng Lin Liu | 2019 | Protein & Cell2019，10，11： | 2 |
| 11 | PD1^+CCR2^+CD8^+T Cells Infiltrate the Central Nervous System during Acute Japanese Encephalitis Virus Infection显示文摘Japanese encephalitis(JE)is a viral encephalitis disease caused by Japanese encephalitis virus(JEV)infection.Uncontrolled inflammatory responses in the central nervous system(CNS)are a hallmark of severe JE.Although the CCR2-CCL2 axis is important for monocytes trafficking during JEV infection,little is known about its role in CNS trafficking of CD8^+T cells.Here,we characterized a mouse model of JEV infection,induced via intravenous injection(i.v.)and delineated the chemokines and infiltrating peripheral immune cells in the brains of infected mice.The CNS expression of chemokines,Ccl2,Ccl3,and Ccl5,and their receptors,Ccr2 or Ccr5.was significantly up-regulated after JEV infection and was associated with the degree of JE pathogenesis.Moreover,JEV infection resulted in the migration of a large number of CD8^+T cells into the CNS.In the brains of JEV-infected mice,infiltrating CD8^+T cells expressed CCR2 and CCR5 and were found to comprise mainly effector T cells(CD44^+CD62L_).JEV infection dramatically enhanced the expression of programmed death 1(PD-1)on infiltrating CD8^+T cells in the brain,as compared to that on peripheral CD8^+T cells in the spleen.This effect was more pronounced on infiltrating CCR2^+CD8^+T cells than on CCR2-CD8^+T cells.In conclusion,we identified a new subset of CD8^+T cells(PD1^+CCR2^+CD8^+T cells)present in the CNS of mice during acute JEV infection.These CD8^+T cells might play a role in JE pathogenesis. | Fang Zhang Linlin Qi Tong Li Xiaojing Li Dan Yang Shengbo Cao Jing Ye Bin Wei | 2019 | Virologica Sinica2019，34，5： | 2 |
| 12 | Dynamics of dissolved organic carbon after a cyanobacterial bloom in hypereutrophie Lake Taihu(China) 显示文摘 | YE Linlin SHI Xiao WU Xiaodong | 2011 | Limnologica2011，41，4： | 1 |
| 13 | Human epididymis protein 4 is a biomarker for transitional cell carcinoma in the urinary system显示文摘 | Xi Z LinLin M Ye T | 2009 | J Clin Lab Anal2009，23，6： | 1 |
| 14 | Generation of developmentally competent oocytes and fertile mice from parthenogenetic embryonic stem cells显示文摘Parthenogenetic embryos,created by activation and diploidization of oocytes,arrest at mid-gestation for defective paternal imprints,which impair placental development.Also,viable offspring has not been obtained without genetic manipulation from parthenogenetic embryonic stem cells(pESCs)derived from parthenogenetic embryos,presumably attributable to their aberrant imprinting.We show that an unlimited number of oocytes can be derived from pESCs and produce healthy offspring.Moreover,normal expression of imprinted genes is found in the germ cells and the mice.pESCs exhibited imprinting consistent with exclusively maternal lineage,and higher X-chromosome activation compared to female ESCs derived from the same mouse genetic background.pESCs differentiated into primordial germ cell-like cells(PGCLCs)and formed oocytes following in vivo transplantation into kidney capsule that produced fertile pups and reconstituted ovarian endocrine function.The transcriptome and methylation of imprinted and X-linked genes in pESC-PGCLCs closely resembled those of in vivo produced PGCs,consistent with efficient reprogramming of methylation and genomic imprinting.These results demonstrate that amplification of germ cells through parthenogenesis faithfully maintains maternal imprinting,offering a promising route for deriving functional oocytes and having potential in rebuilding ovarian endocrine function. | Chenglei Tian Linlin Liu Ming Zeng Xiaoyan Sheng Dai Heng Lingling Wang Xiaoying Ye David L.Keefe Lin Liu | 2021 | Protein & Cell2021，12，12： | 1 |
| 15 | Human epididymis protein 4 isa biomarker for transitional cell carcinoma in the urinarysystem显示文摘 | Xi Z LinLin M Ye T | 2009 | J Clin Lab Anal2009，23，6： | 1 |
| 16 | Human epididymis protein 4 is a biomarker for transitional cell carcinoma in the urinary system 显示文摘 | Xi Z LinLin M Ye T | 2009 | J Clin Lab Anal2009，23，： | 1 |
| 17 | Research of 3D-CFD Simulation on a Large Sized Pit Type Carburizing Frunace显示文摘 | Weimin Zhang Ye Ma and Linlin Li | 2006 | Solid State Phenomena2006，，118： | 1 |
| 18 | Research of 3D - CFD simulation on a large sized pit type carburizing furnace 显示文摘 | Zhang Wei - ming Ma Ye Li Linlin | 2006 | Solid State Phenomena2006，118，： | 1 |
| 19 | Dynamics of dissolved organic carbon after a cyanobacterial bloom in hypereutrophic Lake Taihu (China)显示文摘 | Linlin Ye Xiao Shi Xiaodong Wu Min Zhang Yang Yu Daming Li Fanxiang Kong | 2011 | Limnologica2011，，4： | 1 |
| 20 | Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase显示文摘We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV. | Chao Shan Xiaodan Li Chenglin Deng Baodi Shang Linlin Xu Hanqing Ye Zhiming Yuan Bo Zhang | 2013 | Virologica Sinica2013，28，3： | 1 |
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