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您的检索式：作者名="David L.Keefe"
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| 1 | Generation of developmentally competent oocytes and fertile mice from parthenogenetic embryonic stem cells显示文摘Parthenogenetic embryos,created by activation and diploidization of oocytes,arrest at mid-gestation for defective paternal imprints,which impair placental development.Also,viable offspring has not been obtained without genetic manipulation from parthenogenetic embryonic stem cells(pESCs)derived from parthenogenetic embryos,presumably attributable to their aberrant imprinting.We show that an unlimited number of oocytes can be derived from pESCs and produce healthy offspring.Moreover,normal expression of imprinted genes is found in the germ cells and the mice.pESCs exhibited imprinting consistent with exclusively maternal lineage,and higher X-chromosome activation compared to female ESCs derived from the same mouse genetic background.pESCs differentiated into primordial germ cell-like cells(PGCLCs)and formed oocytes following in vivo transplantation into kidney capsule that produced fertile pups and reconstituted ovarian endocrine function.The transcriptome and methylation of imprinted and X-linked genes in pESC-PGCLCs closely resembled those of in vivo produced PGCs,consistent with efficient reprogramming of methylation and genomic imprinting.These results demonstrate that amplification of germ cells through parthenogenesis faithfully maintains maternal imprinting,offering a promising route for deriving functional oocytes and having potential in rebuilding ovarian endocrine function. | Chenglei Tian Linlin Liu Ming Zeng Xiaoyan Sheng Dai Heng Lingling Wang Xiaoying Ye David L.Keefe Lin Liu | 2021 | Protein & Cell2021，12，12： | 1 |
| 2 | Tet酶调控端粒和基因组稳定性的作用和机制显示文摘Tets家族催化5-甲基胞嘧啶转变为5-羟甲基胞嘧啶。在小鼠胚胎干细胞中单独干扰Tet1或/和Tet2,伴随着端粒重组下降,导致端粒长度缩短和基因组稳定性异常。 | Jiao Yang Renpeng Guo Hua Wang Xiaoying Ye Zhongcheng Zhou Jiameng Dan Haiying Wang Peng Gong Wei Deng Yu Yin Shi Qing Mao Lingbo Wang Junjun Ding Jinsong Li David L.Keefe Meelad M.Dawlaty Jianlong Wang Guo Liang Xu 刘林 | 2017 | 科学新闻2017，19，4： | 0 |
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