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| 1 | Anomalous enhancement of fluorescence of carbon dots through lanthanum doping and potential application in intracellular imaging of ferric ion显示文摘碳点(CD ) 的荧光的异常改进经由做的镧(La ) 被观察。做 La 的 CD (LaCD ) 在 4 min 以内通过微波热分解被准备。与做的 La 3+ ,尽管 La 3+ 是非荧光灯的,排放乐队从蓝色变了到绿色。量收益和荧光一生分别地在大约 20% 和 35% 改善了。所有实验结果显示做的 La 3+ 是调节荧光并且改进 CD 的表演的一个有效方法。LaCD 的另一个唯一的属性是到 Fe 3+ 的高敏感。La-CD-based 荧光探查被建立并且与 91 nmol/L 的察觉的限制为 Fe 3+ 的敏感、选择的察觉使用了。建议荧光探查成功地也被采用通过房间成像在实时 HeLa 房间设想细胞内部的 Fe 3+ 。钇作为 La 展出了一样的荧光改进效果,这也被显示出。结果可以为与特殊性质准备 CD 提供一条新线路。 | Shenghong Yang Xiaohan Sun Zhaoyan Wang Xiayan Wang Guangsheng Guo Qiaosheng Pu | 2018 | Nano Research2018，11，3： | 3 |
| 2 | Recent advances of carbon dots as new antimicrobial agents显示文摘Due to the COVID-19 pandemic,many rapid antimicrobial agents have developed intensively.Carbon dots(CDs),a new type of carbon-based nanomaterials,shows great potential against emerging infectious diseases and antimicrobial-resistant infections due to their unique optical properties,excellent biocompatibility,and easy surface modification.With the definition of the CDs structure and properties,synthesis,and characteristic technology improvement,the research on the CDs as antimicrobial agents has made significant progress.However,the lack of high repeatable and exact preparation methods,and the regular antimicrobial activity make it far from practical application.In this review,we summarize the most recent progress and challenges of CDs antimicrobial.First,an overview of the characteristics and properties is given,and the advantage of CDs applied to antimicrobial is further discussed.Then,it focuses on research progress on antimicrobial mechanisms under different conditions,the critical factors affecting their antimicrobial activity,and the practical antimicrobial applications.Finally,the main challenges and future research perspectives of antimicrobial CDs are proposed. | Pengfei Li Lu Sun Shanshan Xue Dan Qu Li An Xiayan Wang Zaicheng Sun | 2022 | SmartMat2022，3，2： | 3 |
| 3 | Synthesis and characterization of a novel binuclear iron phthalocyanine/reduced graphene oxide nanocomposite for non-precious electrocatalyst for oxygen reduction显示文摘Binuclear iron phthalocyanine/reduced graphene oxide(bi-Fe Pc/RGO) nanocomposite with good electrocatalytic activity for ORR in alkaline medium was prepared in one step. High angle annular dark field image scanning transmission electron microscopy(HAADF-STEM) and energy dispersive X-ray spectroscopy element mapping results show bi-Fe Pc was uniformly distributed on RGO. An obvious cathodic peak located at about-0.23 V(vs. SCE) in CV and an onset potential of-0.004 V(vs. SCE) in LSV indicate the as-prepared bi-Fe Pc/RGO nanocomposite possesses high activity which is closed to Pt/C for ORR. The ORR on bi-Fe Pc/RGO nanocomposite follows four-electron transfer pathway in alkaline medium. Compared with Pt/C, there is only a slight decrease(about 0.02 V vs. SCE) for bi-Fe Pc/RGO nanocomposite when the methanol exists. The excellent activity and methanol tolerance in alkaline solutions proves that bi-Fe Pc/RGO nanocomposite could be considered as a promising cathode catalyst for alkaline fuel cells. | Ruonan Li Dongtang Zhang Yingyan Zhou Xiayan Wang Guangsheng Guo | 2016 | Science China Chemistry2016，59，6： | 3 |
| 4 | Genetic Interactions Reveal that Speci c Defects of Chloroplast Translation are Associated with the Suppression of var2-Mediated Leaf Variegation显示文摘Arabidopsis thaliana L.yellow variegated(var2)mutant is defective in a chloroplast FtsH family metalloprotease,AtFtsH2/VAR2,and displays an intriguing green and white leaf variegation.This unique var2 mediated leaf variegation offers a simple yet powerful tool for dissecting the genetic regulation of chloroplast development.Here,we report the isolation and characterization of a new var2suppressor gene,SUPPRESSOR OF VARIEGATION8(SVR8),which encodes a putative chloroplast ribosomal large subunit protein,L24.Mutations in SVR8suppress var2leaf variegation at ambient temperature and partially suppress the cold induced chlorosis phenotype of var2.Loss of SVR8 causes unique chloroplast rRNA processing defects,particularly the 23S–4.5S dicistronic precursor.The recovery of the major abnormal processing site in svr823S–4.5S precursor indicate that it does not lie in the same position where SVR8/L24 binds on the ribosome.Surprisingly,we found that the loss of a chloroplast ribosomal small subunit protein,S21,results in aberrant chloroplast rRNA processing but not suppression of var2variegation.These ndings suggest that the disruption of speci c aspects of chloroplast translation,rather than a general impairment in chloroplast translation,suppress var2variegation and the existence of complex genetic interactions in chloroplast development. | Xiayan Liu Mengdi Zheng Rui Wang Ruijuan Wang Lijun An Steve R.Rodermel Fei Yu | 2013 | Journal of Integrative Plant Biology2013，55，10： | 3 |
| 5 | Effect on electrochemical reduction of nitrogen to ammonia under ambient conditions: Challenges and opportunities for chemical fuels显示文摘The nitrogen cycle plays an important role in nature,but N-containing products cannot meet human needs.The electrochemical synthesis of ammonia under ambient conditions has attracted the interest of many researchers because it provides a clean and pollution-free synthesis method;however,it has certain difficulties,including a high activation energy,multiple electron transfer,and hydrogenation.Thermodynamic factors limit the selectivity and activity of ammonia synthesis techniques.This review summarizes progress in the electrochemical synthesis of ammonia from theory and experiment.Theoretically,the reduction of nitrogen molecules is analyzed using orbit theory and the thermodynamic reaction pathways.Experimentally,we first discuss the effect of the experimental setup on the nitrogen reduction reaction,and then the four critical of catalysts,including size,electronic,coordination,and orientation effects.These issues must be considered to produce highly-efficient catalysts for electrochemical nitrogen reduction(eNRR).This review provides an overview of the eNRR to enable future researchers to design rational catalysts. | Lijuan Niu Li An Xiayan Wang Zaicheng Sun | 2021 | Journal of Energy Chemistry2021，30，10： | 2 |
| 6 | A pico-HPLC-LIF system for the amplification-free determination of multiple miRNAs in cells显示文摘MicroRNAs are a class of important biomarkers,and the simultaneous detection of multiple miRNAs can provide valuable information about many diseases and biological processes.Amplification-free determination has been developed for the analysis of multiple miRNAs because of its characteristic low cost and high fidelity.Herein,a method for the amplification-free analysis and simultaneous detection of multiple miRNAs based on a so-called pico-HPLC-LIF system is described.In this process,a bare open capilla ry with an inner diameter of 680 nm is used as a sepa ration column for a sample volume of several hundreds of femtoliters(300 fL),followed by separation and detection.The technique has a zeptomolar limit of detection.The method was applied to detect cellular miRNA from adenocarcinomic human alveolar basal epithelial(A549)cell extracts,and the simultaneous detection of the mir-182,miR-155,and let-7 a was achieved.The results showed that the expression of mir-182 and miR-155 was up-regulated and that of let-7 a was down-regulated in A549 cells.This method for multiple miRNAs detection is expected to have broad applications in miRNA-based disease diagnosis,prognosis,treatment,and monitoring. | Wenmei Zhang Zunsheng Han Yingqi Liang Qi Zhang Xiangnan Dou Guangsheng Guo Xiayan Wang | 2021 | Chinese Chemical Letters2021，32，7： | 2 |
| 7 | Surface hydrophobic modification enhanced catalytic performance of electrochemical nitrogen reduction reaction显示文摘Electrocatalytic nitrogen reduction reaction(NRR)is a sustainable approach for NH_(3)production with low energy consumption.However,competing hydrogen reduction reaction(HER)in aqueous solution results in low NH_(3)production and Faraday efficiency(FE).Here,MoS_(2)nanostructures with a hydrophobic surface are synthesized by alkyl thiols modification.Aerophilic and hydrophobic surface facilitates an efficient three-phase contact of N_(2),H_(2)O,and catalyst.Thus,localized concentrated N_(2)molecules can overcome the mass transfer limitation of N2 and depress the HER due to lowering the proton contacts.Although the active-sites decrease with the increase of the alkyl chain since the thiol may cover the active site,the optimized electrocatalyst achieves NH_(3)yield of 12.86×10^(-11)mol·cm^(-2)·s^(-1)at-0.25 V and 22.23%FE,which are 4.3 and 24 times higher than those of MoS2-CP electrocatalyst,respectively.The increased catalytic performance is attributed to the high N_(2)adsorption and depressed HER. | Lijuan Niu Ziwen Liu Guohua Liu Mengxuan Li Xupeng Zong Dandan Wang Li An Dan Qu Xiaoming Sun Xiayan Wang Zaicheng Sun | 2022 | Nano Research2022，15，5： | 2 |
| 8 | Three-dimensional characteristics of mesoscale eddies simulated by a regional model in the northwestern Pacific Ocean during 2000–2008显示文摘Mesoscale eddies play vital roles in ocean processes.Although previous studies focused on eddy surface features and individual three-dimensional(3D)eddy cases in the northwestern Pacific Ocean,the analysis of unique eddy3D regional characteristics is still lacking.A 3D eddy detection scheme is applied to 9 years(2000-2008)of eddyresolving Regional Ocean Modeling System(ROMS)output to obtain a 3D eddy dataset from the surface to a depth of 1000 m in the northwestern Pacific Ocean(15°-35°N,120°-145°E).The 3D characteristics of mesoscale eddies are analyzed in two regions,namely,Box1(Subtropical Countercurrent,15°-25°N,120°-145°E)and Box2(Southern Kuroshio Extension,25°-35°N,120°-145°E).In Box1,the current is characterized by strong vertical shear and weak horizontal shear.In Box2,the current is characterized by the strong Kuroshio,topographic effect,and the westward propagation of Rossby waves.The results indicate the importance of baroclinic instability in Box1,whereas in Box2,both the barotropic and baroclinic instability are important.Moreover,the mesoscale eddies’properties in Box1 and Box2 are distinct.The eddies in Box1 have larger number and radius but a shorter lifetime.By contrast,Box2 has fewer eddies,which have smaller radius but longer lifetime.Vertically,more eddies are detected at the subsurface than at the surface in both regions;the depth of 650 m is the turning point in Box1.Above this depth,the number of cyclonic eddies(CEs)is larger than that of anticyclonic eddies(AEs).In Box2,the number of CEs is dominant vertically.Eddy kinetic energy(EKE)and mean normalized relative vorticity in Box2are significantly higher than those in Box1.With increasing depth,the attenuation trend of EKE and relative vorticity of Box1 become greater than those of Box2.Furthermore,the upper ocean(about 300 m in depth)contains 68.6%of the eddies(instantaneous eddy).Only 16.6%of the eddies extend to 1000 m.In addition,about87%of the eddies are bowl-shaped eddies in the two regions.Only about 3%are cone-shaped eddies.With increasing depth of the eddies,the proportion of bowl-shaped eddies gradually decreases.Conversely,the coneand lens-shaped eddies are equal in number at 700-1000 m,accounting for about 30%each.Studying the 3D characteristics of eddies in two different regions of the northwestern Pacific Ocean is an important stepping stone for discussing the different eddy generation mechanisms. | Guijing Yang Xiayan Lin Guoqing Han Yu Liu Gengxin Chen Jianhui Wang | 2022 | Acta Oceanologica Sinica2022，41，10： | 1 |
| 9 | Synthesis and electrospinning of a novel fluorescent polymer PMMA-PM for quenching-based optical sensing显示文摘 | WANG Xiayan LEE S H KU B C | 2002 | Journal of Macromolecular Science-Pure and Applied Chemistry2002，39，10： | 1 |
| 10 | High-performance self-organized Si nanocomposite anode for lithium-ion batteries显示文摘Silicon is being investigated extensively as an anodic material for next-generation lithium ion batteries for portable energy storage and electric vehicles.However,the large changes in volume during cycling lead to the breakdown of the conductive network in Si anodes and the formation of an unstable solid-electrolyte interface,resulting in capacity fading.Here,we demonstrate nanoparticles with a Si@Mn_(22.6)Si_(5.4)C_4@C double-shell structure and the formation of self-organized Si-Mn-C nanocomposite anodes during the lithiation/delithiation process.The anode consists of amorphous Si particles less than 10 nm in diameter and separated by an interconnected conductive/buffer network,which exhibits excellent charge transfer kinetics and charge/discharge performances.A stable specific capacity of 1100 mAh·g^(-1) at 100 mA·g^(-1) and a coulombic efficiency of 99.2%after 30 cycles are achieved.Additionally,a rate capacity of 343 mAh·g^(-1) and a coulombic efficiency of 99.4%at 12000 mA·g^(-1) are also attainable.Owing to its simplicity and applicability,this strategy for improving electrode performance paves a way for the development of high-performance Si-based anodic materials for lithium ion batteries. | Xiuyun Zhao Dingguo Xia Lin Gu Juncheng Yue Biao Li Hang Wei Huijun Yan Ruqiang Zou Yingxia Wang Xiayan Wang Ze Zhang Jixue Li | 2014 | Journal of Energy Chemistry2014，23，3： | 1 |
| 11 | Electroosmotic pumps for microflow analysis显示文摘 | WANG Xiayan WANG Shili GENDHAR B | 2009 | Trends in Anylytical Chemistry2009，28，1： | 1 |
| 12 | Influence of elution conditions on DNA transport behavior in free solution by hydrodynamic chromatography显示文摘We have previously developed bare narrow-bore capillary chromatography. In this work, high-performance DNA separation was realized for a size range of 10–800 base pairs(bp) utilizing bare narrow-bore capillary chromatography with 750 nm- radius capillaries. Separation behavior of double-stranded DNA(ds DNA) fragments was investigated over a range of eluent concentrations and elution pressures. DNA molecules were hydrodynamically separated in a size-dependent manner in free solution without any sieving matrices, with the longer fragments being eluted out from the capillary earlier. It was found that the eluent concentration variously influenced the transport behavior for different-sized DNA fragments depending upon the configuration of DNA molecules and the association of counterions. Ionic strength of the solutions strongly impacted DNA persistence length. Enhanced elution pressure could shorten analysis time with a slight loss in resolution. Excellent efficiency of two million theoretical plates per meter was achieved, which indicates the enormous potential of bare narrow-bore capillary chromatography for the analysis of DNA fragments. These findings would be useful in understanding the transport behavior of DNA fragments in confined dimensions for chromatography in free solution. | Lei Liu Vijaykumar Veerappan Yuzhi Bian Guangsheng Guo Xiayan Wang | 2015 | Science China Chemistry2015，58，10： | 1 |
| 13 | Fe_(2)Mo_(3)O_(8)/XC-72 electrocatalyst for enhanced electrocatalytic nitrogen reduction reaction under ambient conditions显示文摘To perform the electrochemical nitrogen reduction reaction(NRR)under milder conditions for sustainable ammonia production,electrocatalysts should exhibit high selectivity,activity,and durability.However,the key restrictions are the highly stable N≡N triple bond and the competitive hydrogen evolution reaction(HER),which make it difficult to adsorb and activate N2 on the surface of electrocatalysts,leading to a low ammonia yield and Faraday efficiency.Inspired by the enzymatic nitrogenase process and using the Fe-Mo as the active center,here we report supported Fe_(2)Mo_(3)O_(8)/XC-72 as an effective and durable electrocatalyst for the NRR.Fe_(2)Mo_(3)O_(8)/XC-72 exhibited NRR activity with an NH3 yield of 30.4μg·h^(−1)·mg^(−1)(−0.3 V)and a Faraday efficiency of 8.2%(−0.3 V).Theoretical calculations demonstrated that the electrocatalytic nitrogen fixation mechanism involved the Fe atom in the Fe_(2)Mo_(3)O_(8)/XC-72 electrocatalyst acting as the main active site in the enzymatic pathway(*NH2→*NH3),which activated nitrogen molecules and promoted the NRR performance. | Guohua Liu Lijuan Niu Zhixue Ma Li An Dan Qu Dandan Wang Xiayan Wang Zaicheng Sun | 2022 | Nano Research2022，15，7： | 0 |
| 14 | Development of Ultranarrow-Bore Open Tubular High Efficiency Liquid Chromatography显示文摘Chromatography theory shows that when the inner diameter of the chromatography column is very narrow(e.g.,1—2μm),the maximum efficiency will be produced in liquid chromatography.In addition,the increasing demand for ultrasmall volume sample analysis has produced the trend of narrowing the diameter of the liquid chromatography column.Experiments have verified that ultranarrow-bore open tubular liquid chromatography(i.d.≤2μm)has the advantages of small sample requirement and high separation efficiency.However,there also exist huge challenges along with such obvious advantages.This review summarizes the efforts made by our research group and other research groups to develop this field.We hope that in the near future,ultranarrow bore open-tube liquid chromatography can be successfully and maturely applied to the application of analytes with ultrasmall size and volume such as single-cell and even subcellular organelles omics research. | Yingyan Zhou Guangsheng Guo Xiayan Wang | 2022 | Chinese Journal of Chemistry2022，40，1： | 0 |
| 15 | Transparent Coating with TiO2 Nanorods for High-performance Photocatalytic Self-cleaning and Environmental Remediation显示文摘High-performance self-cleaning coatings are highly desired by the industry and market.Herein,we synthesized two kinds of ultrafine TiO2 nanocrystals,one is anatase dots with a diameter of 5 nm,and the other is rutile rods with 1.5 nm in width and 7 nm in length.The prepared TiO:nanocrystal is highly dispersed and stable in water over a month.The coating can be fabricated via a simple spraving method,displaying excellent optical properties and photocatalytic performance on self-cleaning and surrounding environment remediation.The transmittance of glass remains 80%-90%for visible and near-infrared light after 30 cycles of spray.RhB solution(50 mg/L)was applied to the coating surface and form a solid RhB layer was formed,which can be completely removed in 30 min's light irradiation.RhB aqueous solution(100 mL,5 mg/L)was purified after 180 min by a 10 cm×10 cmglass,on which the coating was sprayed 30 times.The concentrations of formaldehyde and PM2.5 in surrounding air displayed a significant decrease along 50 min's monitoring.This high-performance coating shows great potential for constructing functional coating on various substrates for industrial applications. | JIANG Wenshuai ZONG Xupeng WANG Xiayan SUN Zaicheng | 2020 | Chemical Research in Chinese Universities2020，36，6： | 0 |
| 16 | The critical factors of photocatalytic H2 production from seawater by using TiO_(2)as photocatalyst显示文摘The solar H_(2)generation directly from natural seawater is a sustainable way of green energy.However,it is limited by a low H_(2)generation rate even compared to fresh water.In this report,TiO_(2)is chosen as a model photocatalyst to disclose the critical factor to deteriorate the H_(2)generation rate from seawater.The simulated seawater(SSW),which is composed of eight ions(Na^(+),K^(+),Ca^(2+),Mg^(2+),Cl^(−),Br^(−),SO_(4)^(2−),and CO_(3)^(2−)),is investigated the effect of each ion on the H_(2)production.The results indicate that all ions have a negative effect at the same concentration as in the seawater except Br−.The CO_(3)^(2−)has the most serious deterioration,and the H_(2)production rate lowers near 40%even at[CO_(3)^(2−)]of 1.5 mmol·L^(−1).The H_(2)production rate can be recovered to 85%if the CO_(3)^(2−)is excluded from the SSW.To understand the reason,the zeta potential of the TiO_(2)treated with different ions aqueous solution reveals that the zeta potential decreases when it is treated with CO_(3)^(2−)and SO_(4)^(2−)due to they can adsorb on the surface of TiO_(2)nanoparticles.Fourier transform infrared(FTIR)and thermogravimetric analysis-mass spectroscopy(TGA-MS)further confirm that the adsorbed ion is mainly from CO_(3)^(2−).Since the pH of seawater is about 8.9 between pKa1(6.37)and pKa2(10.3)of H_(2)CO3,the CO_(3)^(2−)should exist in the form of HCO3−in the seawater.We proposed a simple method to remove the adsorbed HCO3−from the TiO_(2)surface by adjusting the pH below the pKa1.The results indicate that if a trace amount of HCl(adjusting pH~6.0)is added to the SSW,the H_(2)production rate can be recovered to 85%of that in pure water. | Yaqian He Pengfei Li Wenning Liu Li An Dan Qu Xiayan Wang Zaicheng Sun | 2023 | Nano Research2023，16，4： | 0 |
| 17 | Single-cell mass spectrometry studies of drug metabolism heterogeneity and primary resistance to gefitinib in non-small cell lung cancer cells显示文摘Patients with epidermal growth factor receptor(EGFR)wild-type non-small cell lung cancer(NSCLC)often show primary resistance to gefitinib therapy.It is thus necessary to study the metabolism of gefitinib in NSCLC cells to comprehensively reveal the reasons for the primary resistance of tumors.Herein,we develop a platform for studying drug metabolism heterogeneity based on single-cell mass spectrometry(sDMH-scMS)by integrating living-cell electrolaunching ionization MS(ILCEI-MS)and high-performance liquid chromatography-MS(HPLC-MS)analysis,and the primary resistance of NSCLC cells to gefitinib was studied using this platform.The ILCEI-MS analysis showed that approximately 11.9%of NSCLC single cells contained the gefitinib metabolite M11;HPLC-MS detection diluted the intensity of M11 in subpopulations and concealed the heterogeneity of drug metabolism in tumor single cells.The intensity of gefitinib in EGFR wild-type A549 cells was markedly lower than in mutant PC9 cells,and the intensity of gefitinib metabolites was significantly higher than in PC9 cells,suggesting that the primary resistance of NSCLC cells is related to gefitinib metabolism.Moreover,the combination of gefitinib and the drug-metabolizing enzyme inhibitorα-naphthoflavone was shown to overcome the primary resistance of the NSCLC cells.Overall,the results of this study are expected to be applicable for clinical drug resistance diagnosis and treatment at the single-cell level. | Guizhen Zhu Yaoyao Zhao Wenmei Zhang Yuanyuan Wu Yuanxing Liu Guangsheng Guo Xiayan Wang Zhihong Liu | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
| 18 | Degradation properties of fulvic acid and its microbially driven mechanism from a partial nitritation bioreactor through multi-spectral and bioinformatic analysis显示文摘This study employed multispectral techniques to evaluate fulvic acid(FA)compositional characteristic and elucidate its biodegradation mechanisms during partial nitritation(PN)process.Results showed that FA removal efficiency(FRE)decreased from 90.22 to 23.11%when FA concentrations in the reactor were increased from 0 to 162.30 mg/L,and that molecular size,degree of aromatization and humification of the effluent FA macromolecules all increased after treatment.Microbial population analysis indicated that the proliferation of the Comamonas,OLB12 and Thauera exhibit high FA utilization capacity in lower concentrations(<50.59 mg/L),promoting the degradation and removal of macromolecular FA.In addition,the sustained increase in external FA may decrease the abundance of above functional microorganisms,resulting in a rapid drop in FRE.Furthermore,from the genetic perspective,the elevated FA levels restricted carbohydrate(ko00620,ko00010 and ko00020)and nitrogen(HAO,AMO,NIR and NOR)metabolism-related pathways,thereby impeding FA removal and total nitrogen loss associated with N_(2)O emissions. | Quanhao Dou Li Zhang Tingjun Dong Zixuan Song Xuepeng Fan Yongzhen Peng Xiayan Wang Jiachun Yang | 2024 | Journal of Environmental Sciences2024，，1： | 0 |
| 19 | Formation and fluorescent mechanism of red emissive carbon dots from o-phenylenediamine and catechol system显示文摘Carbon dots(CDs)as the advancing fluorescent carbon nanomaterial have superior potential and prospective.However,the ambiguous photoluminescence(PL)mechanism and intricate structure-function relationship become the greatest hindrances in the development and applications of CDs.Herein,red emissive CDs were synthesized in high yield from O-phenylenediamine(oPD)and catechol(CAT).The PL mechanism of the CDs is considered as the molecular state fluorophores because 5,14-dihydroquinoxalino[2,3-b]phenazine(DHQP)is separated and exhibits the same PL properties and behavior as the CDs.These include the peak position and shape of the PL emission and PL excitation and the emission dependence on pH and solvent polarity.Both of them display close PL lifetime decays.Based on these,we deduce that DHQP is the fluorophore of the red emissive CDs and the PL mechanism of CDs is similar to DHQP.During the PL emission of CDs,the electron of the molecule state can transfer to CDs.The formation process of DHQP is further confirmed by the reaction intermediates(phthalazine,dimers)and oPD.These findings provide insights into the PL mechanism of this type of CDs and may guide the further development of tunable CDs for tailored properties. | Pengfei Li Shanshan Xue Lu Sun Xupeng Zong Li An Dan Quo Xiayan Wang Zaicheng Sun | 2022 | Light(Science & Applications)2022，11，11： | 0 |
| 20 | Ultra-thin temperature controllable microwell array chip for continuous real-time high-resolution imaging of living single cells显示文摘Single-cell imaging,a powerful analytical method to study single-cell behavior,such as gene expression and protein profiling,provides an essential basis for modern medical diagnosis.The coding and localization function of microfluidic chips has been developed and applied in living single-cell imaging in recent years.Simultaneously,chip-based living single-cell imaging is also limited by complicated trapping steps,low cell utilization,and difficult high-resolution imaging.To solve these problems,an ultra-thin temperature-controllable microwell array chip(UTCMA chip)was designed to develop a living single-cell workstation in this study for continuous on-chip culture and real-time high-resolution imaging of living single cells.The chip-based on ultra-thin ITO glass is highly matched with an inverted microscope(or confocal microscope)with a high magnification objective(100×oil lens),and the temperature of the chip can be controlled by combining it with a home-made temperature control device.High-throughput single-cell patterning is realized in one step when the microwell array on the chip uses hydrophilic glass as the substrate and hydrophobic SU-8 photoresist as the wall.The cell utilization rate,single-cell capture rate,and microwell occupancy rate are all close to 100%in the microwell array.This method will be useful in rare single-cell research,extending its application in the biological and medical-related fields,such as early diagnosis of disease,personalized therapy,and research-based on single-cell analysis. | Yuanyuan Wu Lei Zhao Yaran Chang Liang Zhao Guangsheng Guo Xiayan Wang | 2021 | Chinese Chemical Letters2021，32，11： | 0 |
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