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1篇 您的检索式:作者名="Steve R.Rodermel"
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1Genetic Interactions Reveal that Speci c Defects of Chloroplast Translation are Associated with the Suppression of var2-Mediated Leaf Variegation显示文摘Arabidopsis thaliana L.yellow variegated(var2)mutant is defective in a chloroplast FtsH family metalloprotease,AtFtsH2/VAR2,and displays an intriguing green and white leaf variegation.This unique var2 mediated leaf variegation offers a simple yet powerful tool for dissecting the genetic regulation of chloroplast development.Here,we report the isolation and characterization of a new var2suppressor gene,SUPPRESSOR OF VARIEGATION8(SVR8),which encodes a putative chloroplast ribosomal large subunit protein,L24.Mutations in SVR8suppress var2leaf variegation at ambient temperature and partially suppress the cold induced chlorosis phenotype of var2.Loss of SVR8 causes unique chloroplast rRNA processing defects,particularly the 23S–4.5S dicistronic precursor.The recovery of the major abnormal processing site in svr823S–4.5S precursor indicate that it does not lie in the same position where SVR8/L24 binds on the ribosome.Surprisingly,we found that the loss of a chloroplast ribosomal small subunit protein,S21,results in aberrant chloroplast rRNA processing but not suppression of var2variegation.These ndings suggest that the disruption of speci c aspects of chloroplast translation,rather than a general impairment in chloroplast translation,suppress var2variegation and the existence of complex genetic interactions in chloroplast development.Xiayan Liu Mengdi Zheng Rui Wang Ruijuan Wang Lijun An Steve R.Rodermel Fei Yu 2013Journal of Integrative Plant Biology2013,55,10:3
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