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| 1 | Why choose 3D bioprinting? Part I: a brief introduction of 3D bioprinting for the beginners显示文摘Graphic abstract Concept of 3D bioprinting 3D printing,also called additive manufacturing,is a layerby-layer manufacturing method,and it has been implemented in a wide variety of areas.3D printing could be treated as the reverse process of potato cutting,automatically assembling the chips or slicing into a potato[1].When this simple idea is met with biomedical engineering,3D bioprinting is born. | Yong He Mingjun Xie Qing Gao Jianzhong Fu | 2019 | Bio-Design and Manufacturing2019，2，4： | 4 |
| 2 | Bioprinting of novel 3D tumor array chip for drug screening显示文摘Biomedical field has been seeking a feasible standard drug screening system consisting of 3D tumor model array for drug researching due to providing sufficient samples and simulating actual in vivo tumor growth situation,which is still a challenge to rapidly and uniformly establish though.Here,we propose a novel drug screening system,namely 3D tumor array chip with“layer cake”structure,for drug screening.Accurate gelatin methacryloyl hydrogel droplets(~0.1μL)containing tumor cells can be automatically deposited on demand with electrohydrodynamic 3D printing.Transparent conductive membrane is introduced as a chip basement for preventing charges accumulation during fabricating and convenient observing during screening.Culturing chambers formed by stainless steel and silicon interlayer is convenient to be assembled and recycled.As this chip is compatible with the existing 96-well culturing plate,the drug screening protocols could keep the same as convention.Important properties of this chip,namely printing stability,customizability,accuracy,microenvironment,tumor functionalization,are detailly examined.As a demonstration,it is applied for screening of epirubicin and paclitaxel with breast tumor cells to confirm the compatibility of the proposed screening system with the traditional screening methods.We believe this chip will potentially play a significant role in drug evaluation in the future. | Mingjun Xie Qing Gao Jianzhong Fu Zichen Chen Yong He | 2020 | Bio-Design and Manufacturing2020，3，3： | 3 |
| 3 | The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.显示文摘Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria sp.Numerous‘Huaiju 2#’plants were inoculated with Alternaria sp.for three or five days.Metabolic analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the control.Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants.RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads.Among these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp.infection,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling pathways.In particular,SA signaling was highly responsive to Alternaria sp.infection.The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data.One candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgenic plants to black spot.These findings indicate that the SA response pathway is likely involved in the defense of‘Huaiju 2#’against Alternaria sp.pathogens. | Xiting Zhao Lingyu Song Liwei Jiang Yuting Zhu Qinghui Gao Dandan Wang Jing Xie Meng Lv Ping Liu Mingjun Li | 2020 | Horticulture Research2020，7，1： | 3 |
| 4 | Short-channel effects on the static noise margin of 6T SRAM composed of 2D semiconductor MOSFETs显示文摘This paper investigates the influence of the short-channel effects(SCEs) on the static noise margin(SNM) of 6 T(6 transistors) SRAM composed of 2 D MOSFETs. An analytical all-region I-V model for short-channel complementary 2 D MOSFETs has been developed, and a simulation model has been built to calculate SNM with the consideration of SCEs and velocity saturation. The results show that there exists an optimal value of channel length(L_(opt)) where SNM reaches a maximum, and Lopt is approximately three times the scale length. In the region where L>L_(opt), SNM increases slightly as L decreases because of velocity saturation, while in the region where L | Qian XIE Chen CHEN Mingjun LIU Shuang XIA Zheng WANG | 2019 | Science China(Information Sciences)2019，62，6： | 3 |
| 5 | All-optical controlled-NOT logic gate achieving directional asymmetric transmission based on metasurface doublet显示文摘Optical logic gates play important roles in all-optical logic circuits,which lie at the heart of the next-generation optical computing technology.However,the intrinsic contradiction between compactness and robustness hinders the development in this field.Here,we propose a simple design principle that can possess multiple-input-output states according to the incident circular polarization and direction based on the metasurface doublet,which enables controlled-NOT logic gates in infrared region.Therefore,the directional asymmetric electromagnetic transmission can be achieved.As a proof of concept,a spin-dependent Janus metasurface is designed and experimentally verified that four distinct images corresponding to four input states can be captured in the far-field.In addition,since the design method is derived from geometric optics,it can be easily applied to other spectra.We believe that the proposed metasurface doublet may empower many potential applications in chiral imaging,chiroptical spectroscopy and optical computing. | Yijia Huang Tianxiao Xiao Shuai Chen Zhengwei Xie Jie Zheng Jianqi Zhu Yarong Su Weidong Chen Ke Liu Mingjun Tang Peter Müller-Buschbaum Ling Li | 2023 | Opto-Electronic Advances2023，6，7： | 2 |
| 6 | Why choose 3D bioprinting?Part II:methods and bioprinters显示文摘Classification of 3D bioprinting As we mentioned in the last editorial,3D printing,also known as additive manufacturing,could be considered as the reverse process of potato cutting,automatically assembling sliced potato,shredded potato,diced potato to integrity[1].Generally speaking,cell-laden 3D bioprinting can be classified into three types:extrusion-based,droplet-based and photocuring-based bioprinting according to different printing principles.Extrusion-based bioprinting squeezes out continuous hydrogel fibers to set up structures;dropletbased bioprinting generates droplets as the basic unit for biofabrication;and photocuring-based bioprinting utilizes the characteristics of light-sensitive materials,to stack 3D models layer-by-layer.Different bioprinting approaches own diverse characteristics facing various scenarios and have specific requirements for bioinks. | Yong He Zeming Gu Mingjun Xie Jianzhong Fu Hui Lin | 2020 | Bio-Design and Manufacturing2020，3，1： | 2 |
| 7 | Sandwich Immunoassay for Hepatitis C Virus Non-Structural 5A Protein Using a Glassy Carbon Electrode Modified with an Au-MoO3/Chitosan Nanocomposite显示文摘 | Mo Liang Li Wang Cuixia Ma Mingjun Zhang Guoming Xie | 2013 | Analytical Letters2013，，8： | 1 |
| 8 | Effects of RNAi-mediated gene silencing of LRIG3 expression on cell cycle and survival of glioma cells显示文摘 | Mingjun Cai Ruifan Xie Lin Han Rudong Chen Baofeng Wang Fei Ye Dongsheng Guo Ting Lei | 2009 | Journal of Huazhong University of Science and Technology2009，，1： | 1 |
| 9 | Realization Path to Pro-Poor-Tourism Development of Dabie Mountains显示文摘Relying on rich tourism resources of the Dabie Mountains to accelerate industrialized poverty alleviation and development is the inevitable choice of promoting regional economic and social leapfrog development. The realistic basis of pro-poor tourism of the Dabie Mountains is already ready and continuously strengthened. This paper, based on domestic and foreign pro-poor tourism practices, put forward the development path to pro-poor-tourism of Dabie Mountains of 'establishing tourism development platform with the government as the leading factor; establishing the benefit sharing mechanism of pro-poor tourism with community participation as the main line; improving the community residents' professionalism in engaging in tourism with local residents as the main body; enhancing the overall effectiveness of pro-poor tourism with tourism as the principal industry'. | XIE Mingjun JIANG Weijun MA Yingshuang LUO Fang WANG Qing | 2017 | Journal of Landscape Research2017，9，4： | 1 |
| 10 | Rapid and mass manufacturing of soft hydrogel microstructures for cell patterns assisted by 3D printing显示文摘Micro-/nano-patterns on hydrogels are widely used in cell patterning.However,manufacturing molds with traditional lithography is time-consuming and expensive.In addition,the excessive demolding force can easily damage patterns since biocompatible hydrogels are ultra-soft or brittle.Here,we presented a novel method for rapid and mass fabrication of cell patterns.High-precision three-dimensional(3D)printing was used to manufacture a mold with a resolution of 2µm,and the gelatin-based hydrogel was cured by thermal–photo-crosslinking so that the low-concentration and low-substitutionrate hydrogel could be demolded successfully.We found that pre-cooling before illumination made gelatin-based hydrogels resilient due to the partial regain of triple-helix structures.With this method,arbitrarily customized hydrogel patterns with a feature size of 6–80µm can be fabricated stably and at low cost.When cardiomyocytes were seeded on ultra-soft hydrogels with parallel groove structures,a consistent and spontaneous beating with 216 beats per minute(BPM)could be observed,approaching the natural beating rate of rat hearts(300 BPM).Overall,this work provides a general scheme for manufacturing cell patterns which has great potential for cell ethology and tissue repair. | Chaofan He Xuechun Chen Yuan Sun Mingjun Xie Kang Yu Jing He Jinwei Lu Qing Gao Jing Nie Yi Wang Yong He | 2022 | Bio-Design and Manufacturing2022，5，4： | 0 |
| 11 | Combining methylated SEPTIN9 and RNF180 plasma markers for diagnosis and early detection of gastric cancer显示文摘Dear Editor,Early diagnosis is critical for successful treatment of gastric adenocarcinoma(GA).However,the sensitivities of tumor markers carcinoembryonic antigen(CEA),cancer antigen 19-9(CA19-9)and CA72-4 for GA detection are approximately 20%[1],and the sensitivities of all markers combined for early gastric cancer detection is still very low[2].DNA methylation plays a major role in tumorigenesis and therefore has obvious potential as a non-invasive biomarker for cancer detection[3].Through genome-wide methylation analysis and histological verification,we previously identified ring finger protein 180(RNF180)as a novel preferentially methylated gene in GA[4,5]. | Yongzhan Nie Xianchun Gao Xiqiang Cai Zhen Wu Qiaoyi Liang Guobing Xu Na Liu Peng Gao Jingyu Deng Hongzhi Xu Zhanlong Shen Changqi Cao Fenrong Chen Nannan Zhang Yongxi Song Mingjun Sun Chengyin Liu Guangpeng Zhou Weili Han Jianhua Dou Huahong Xie Liping Yao Zhiguo Liu Gang Ji Xin Wang Qingchuan Zhao Lei Shang Daiming Fan Xiaoliang Han Jianlin Ren Han Liang Zhenning Wang Jinhai Wang Qi Wu Jun Yu Kaichun Wu the MAGIS Study Group | 2023 | Cancer Communications2023，43，11： | 0 |
| 12 | Facile 3D cell culture protocol based on photocurable hydrogels显示文摘Introduction Why 3D cell culture urgently needed?Tissue engineering has extremely influenced the development process of basic biological studies and biomedical technology.For quite a long time,the vast majority of these researches have been relying on the experimental results of conventional two-dimensional(2D)cell culture in flask,petri dish or well plate.However,these 2D culture results could be very different with or even totally opposite to the actual situations in vivo where cells grow inside a 3D extracellular matrix(ECM).Therefore,3D cell culture has been playing a more and more significant role because of its higher accuracy and authenticity[1–4]. | Mingjun Xie Yating Zheng Qing Gao Yong He | 2021 | Bio-Design and Manufacturing2021，4，1： | 0 |
| 13 | A bionic controllable strain membrane for cell stretching at air–liquid interface inspired by papercutting显示文摘Lung diseases associated with alveoli,such as acute respiratory distress syndrome,have posed a long-term threat to human health.However,an in vitro model capable of simulating different deformations of the alveoli and a suitable material for mimicking basement membrane are currently lacking.Here,we present an innovative biomimetic controllable strain membrane(BCSM)at an air–liquid interface(ALI)to reconstruct alveolar respiration.The BCSM consists of a high-precision three-dimensional printing melt-electrowritten polycaprolactone(PCL)mesh,coated with a hydrogel substrate—to simulate the important functions(such as stiffness,porosity,wettability,and ALI)of alveolar microenvironments,and seeded pulmonary epithelial cells and vascular endothelial cells on either side,respectively.Inspired by papercutting,the BCSM was fabricated in the plane while it operated in three dimensions.A series of the topological structure of the BCSM was designed to control various local-area strain,mimicking alveolar varied deformation.Lopinavir/ritonavir could reduce Lamin A expression under over-stretch condition,which might be effective in preventing ventilator-induced lung injury.The biomimetic lung-unit model with BCSM has broader application prospects in alveoli-related research in the future,such as in drug toxicology and metabolism. | Yuanrong Li Mingjun Xie Shang Lv Yuan Sun Zhuang Li Zeming Gu Yong He | 2023 | International Journal of Extreme Manufacturing2023，5，4： | 0 |
| 14 | Why choose 3D bioprinting?Part Ⅲ:printing in vitro 3D models for drug screening显示文摘Dilemmas of current drug screening Recently,multiple technological advancements have been involved in the process of drug research and discovery.In addition,the spending on the relevant projects has doubled in the last 20 years,cumulatively increasing 28%from 2014 to 2016.However,these improvements have not led directly to an obvious increase in feasible drug candidates that successfully passed the clinical trials.According to the increased spending and stagnant clinical success rate of 10–20%,the efficiency of this project drastically decreased from around 40 drugs per billion in 1950 to 0.6 drugs in 2012. | Yong He Jing Nie Mingjun Xie Qing Gao | 2020 | Bio-Design and Manufacturing2020，3，3： | 0 |
| 15 | Rheology and microstructure effects of waste spent coffee grounds in modifying asphalt binder显示文摘本文将咖啡萃取后得到的固体废弃物咖啡渣(Spent coffee grounds,SCGs)应用于沥青材料改性中,旨在实现生物基固废材料处理和沥青路面性能提升的双重目的。通过傅里叶红外光谱(FTIR)、差示扫描量热仪(DSC)、动态剪切流变仪(DSR)和布氏黏度计等技术,证实了咖啡渣在路面材料可持续化发展中具有潜在的应用与推广前景。试验结果表明,咖啡渣改性过程主要基于物理增强作用;与基质沥青相比,合理掺量的咖啡渣改性沥青具有理想的高温稳定性和良好的抗永久变形能力;同时1%和3%掺量的咖啡渣引入有利于增强基质沥青的低温性能。然而,过多咖啡渣的加入可能会对改性沥青的流变性能产生负面影响,这表明改性时应选择合理掺量的咖啡渣改性剂以满足实际性能提升需求。 | Mingjun Xie Linglin Xu Kai Wu Yutong Wen Hongmi Jiang Zhengwu Jiang | 2023 | Low-carbon Materials and Green Construction2023，1，1： | 0 |
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