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| 1 | Methods for the extraction and RNA profiling of exosomes显示文摘AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques. | Emily Zeringer Mu Li Tim Barta Jeoffrey Schageman Ketil Winther Pedersen Axl Neurauter Susan Magdaleno Robert Setterquist Alexander V Vlassov | 2013 | World Journal of Methodology2013，3，1： | 5 |
| 2 | 堆积物古DNA揭示西藏廓雄遗址的食物构成显示文摘动物考古和植物考古是重建古代食谱的重要手段.对出土动植物遗存的种属鉴定是动植物考古研究的基础,但其鉴定效率严重依赖于动植物遗存的保存状态.沉积物古DNA(sedimentary ancient DNA)是一种新兴的技术手段,可以从数十万年以来的沉积物中提取出古代动植物和微生物的遗传信息,进而恢复当时生态环境中的物种组成.但目前将沉积物古DNA技术应用于旷野遗址的研究还鲜有报道.本文尝试将沉积物古DNA技术应用于西藏廓雄遗址(3160~2954cal a BP)的调查剖面,并与出土的动植物遗存进行对比.结果发现,在大麦(Hordeum vulgare)和绵羊(Ovis aries)等农作物与家养动物的鉴定上,两种方法可以互相印证,但遗址堆积物古DNA中还检测到大植物遗存中没有发现的粟(Setaria italica),以及多种与农作物伴生的微生物.本研究通过动植物常规鉴定方法与沉积物古DNA分析,揭示了廓雄遗址先民的部分食物组成,其中包括:粟类、麦类等谷物,绵羊、黄牛等家养动物,以及渔猎的野生动物等.本项研究表明,沉积物古DNA技术具有通过旷野遗址文化堆积物复原其中动植物种属信息的潜力,为其进一步应用于植物考古和动物考古研究奠定了基础. | 顾政权 高玉 王一如 杨继帅 冉景坤 杨晓燕 夏格旺堆 Mikkel Winther PEDERSEN 盛桂莲 王昱程 陈发虎 | 2023 | 中国科学:地球科学2023，53，4： | 4 |
| 3 | Tumor suppressor ASXL1 is essential for the activation of INK4B expression in response to oncogene activity and anti-proliferative signals显示文摘ASXL1 变化经常在 hematological 肿瘤被发现,并且 Asxl1 的损失在老鼠支持 myeloid 转变。这里我们在 vivo 在在 Histone H2A (H2AK119ub1 ) 上的 mono-ubiquitylated 离氨酸 119 的 deubiquitylation 为 ASXL1-BAP1 建筑群支持一个角色的现在的数据。Polycomb 组蛋白质在正常开发期间控制 INK4B-ARF-INK4A 地点的表达式,部分地通过催化 H2AK119 的 mono-ubiquitylation。自从地点的激活, INK4B-ARF-INK4A 玩一设有自动保险装置机制免于 tumorigenesis,我们调查了 ASXL1 依赖的 H2A deubiquitylation 是否在它的激活起一个作用。有趣地,我们发现 ASXL1 明确地为 p15 响应 oncogenic 发信号和外来的 anti-proliferative 的 INK4B 发信号。因为我们发现 ASXL1 和 BAP1 两个都在 INK4B 地点被充实,我们的结果建议 INK4B 地点的激活要求 H2AK119ub1 的 ASXL1/BAP1-mediated deubiquitylation。一致地,我们 ASXL1 变化与被联系的结果表演降低 p15 INK4B 和在主要骨头髓房间的造血的祖先的一个 proliferative 优点,和在多重房间线的 ASXL1 的那弄空导致抵抗到生长禁止的信号。一起拿,这研究连接 INK4B 表示的调停 ASXL1 的 H2A deubiquitylation 和 transcriptional 激活到它的肿瘤 suppressor 函数。 | Xudong Wu Ida Holst Bekker-Jensen Jesper Christensen Kasper Dindler Rasmussen Simone Sidoli Yan Qi Yu Kong Xi Wang Yajuan Cui Zhijian Xiao Guogang Xu Kristine Williams Juri Rappsilber Casper Kaae Sonderby Ole Winther Ole N Jensens Kristian Helin | 2015 | Cell Research2015，25，11： | 3 |
| 4 | 16 S rRNA survey revealed complex bacterial communities and evidence of bacterial interference on human adenoids显示文摘 | Tiantian Ren Dominique Ulrike Glatt Tam Nhu Nguyen Emma Kaitlynn Allen Stephen V. Early Michele Sale Birgit Winther Martin Wu | 2012 | Environ Microbiol2012，，2： | 2 |
| 5 | Endothelial Cell-Specific NF-κB Inhibition Protects Mice from Atherosclerosis显示文摘 | Ralph Gareus Elena Kotsaki Sofia Xanthoulea Ingeborg van der Made Marion J.J. Gijbels Rozina Kardakaris Apostolos Polykratis George Kollias Menno P.J. de Winther Manolis Pasparakis | 2008 | Cell Metabolism2008，，5： | 2 |
| 6 | Posterior capsule opacification and Nd: YAG Capsulotomy with heparin -surface -modified intraoc- ular lenses显示文摘 | Winther - Nielsen A Johansen J Pedersen G K | 1998 | J Cataract Surg1998，24，： | 1 |
| 7 | Nuclear factor kappaB signaling in atherogenesis显示文摘 | Kanters E Kraal G | 2005 | Arterioscler Thromb Vasc Biol2005，25，5： | 1 |
| 8 | Effect of human scavenger receptor class A overexpression in bone marrow-derived cells on cholesterol levels and atherosclerosis in ApoE- deficient mice 显示文摘 | van Eck M de Winther MP Herijgers N | 2000 | Arterioscler Thromb Vasc Biol2000，20，12： | 1 |
| 9 | A bibliometric analy- sis of the research utilization literature in nursing 显示文摘 | Estabrooks CA Winther C Derksen L | 2004 | Nurs- ing Research2004，53，5： | 1 |
| 10 | Review: biosynthesis and function of yeast vacuolar proteases 显示文摘 | Van Den Hazel HB Kiellan-Brandt MC Winther JR | 1996 | Yeast1996，12，1： | 1 |
| 11 | Long-term risk of cancer in ulcerative colitis: A population-based cohort study from Copenhagen County显示文摘 | Karen V. Winther Tine Jess Ebbe Langholz Pia Munkholm Vibeke Binder | 2004 | Clinical Gastroenterology and Hepatology2004，，12： | 1 |
| 12 | Hydration and Mobility of Trehalose in Aqueous Solution显示文摘 | Winther L R Qvist J Halle B | 2012 | The Journal of Physical Chemistry B2012，116，： | 1 |
| 13 | Long-term risk of cancer in ulcerative colitis: A population-based cohort study from Copenhagen County显示文摘 | Karen V. Winther Tine Jess Ebbe Langholz Pia Munkholm Vibeke Binder | 2004 | Clinical Gastroenterology and Hepatology2004，，12： | 1 |
| 14 | New national emission inventory for navigation in Denmark显示文摘 | Winther M | 2008 | Atmospheric Environment2008，42，： | 1 |
| 15 | Myeloperoxidase: molecular mechanisms of action and their relevance to human health and disease显示文摘 | van der Veen BS de Winther MP Heeringa P | 2009 | Antioxid Redox Signal2009，11，11： | 1 |
| 16 | 综合海洋管理 和谐人海关系显示文摘一、引言海洋关乎人类生存与可持续发展,既是支撑全球未来发展的战略空间,也是全球经济增长的重要新引擎。据经济合作与发展组织2016年统计,2010年全球海洋产业(包括海洋清洁能源、渔业、海运海工、海洋旅游等)总产值约为1.5万亿美元,预计到2030年,海洋经济将超过3万亿美元。由此,海洋、气候变化和生物多样性已共同成为当下联合国和各国政府密切关注的三大热点问题。 | 戴民汉(文/图) 王贵华(文/图) Jan-Gunnar Winther(文/图) Eliza Northrop(文/图) Ines Aguiar Branco(文/图) | 2020 | 世界环境2020，，4： | 1 |
| 17 | Robust multi-scale Clustering of Large DNA Microarray Datasets with the Consensus Algorithm显示文摘 | Grotkjaer T Winther O Regenberg B Nielsen J Hansen LK | 2006 | BIOINFORMAT ICS2006，22，1： | 1 |
| 18 | The structural basis of calcium transport by the calcium pump 显示文摘 | Olesen C Picard M Winther AM | 2007 | Nature2007，450，7172： | 1 |
| 19 | Molecular pathways reg-ulating macrophage polarization : implications for atherosclerosis 显示文摘 | Hoeksema MA Stoger JL de Winther MP | 2012 | Curr Atheroscler Rep2012，14，3： | 1 |
| 20 | Surprisingly high stability of barley lipid transfer protein, LTP1, towards denaturant, heat and proteases 显示文摘 | LINDORFF L K WINTHER J R | 2001 | Fehs Lett2001，488，3： | 1 |
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