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| 1 | Role of AXL in invasion and drug resistance of colon and breast cancer cells and its association with p53 alterations显示文摘AIM To characterize AXL receptor tyrosine kinase(AXL)expression in relationship to tumor protein P53(TP53gene,p53 protein)and its role in tumor invasion and response to therapy.METHODS We used 14 cell lines,including 3 isogenic pairs carrying mutant/knockout p53,to gain insight into the relationship between AXL and TP53.These included HCT116,HCT116.p53 mutant,RKO,and RKO.p53-/-lines(all from colon cancers)as well as breast cancer cell lines MCF7 and 1001(MCF7-p53 mutant clone).He La cell line was used as a positive control for epithelial to mesenchymal transition(EMT).AXL expression was determined by Western blotting using rabbit monoclonal antibody clone C89E7.AXL si RNA silencing was performed and followed by collagen invasion assay.Cell viability analysis using the sulforhodamine B assay and the invasion assay were performed after exposure to chemotherapeutic agents(doxorubicin for breast cancer cells;5FU or irinotecan for colon cancer cells).RESULTS We showed that the introduction of p53 mutations or knockout increased expression levels of AXL in isogenic cells compared to the matching p53 wild-type parental cells.Overall,we found a trend for correlation between the potential EMT candidate AXL,p53 alterations,and EMT markers in colorectal and breast cancers.The expression of AXL in RKO cells,a rare colon cancer cell line with inactive Wnt signaling,suggests that the AXL oncogene might provide an alternative genetic pathway for colorectal carcinogenesis in the absence of Wnt signaling activation and TP53 mutation.AXL silencing in the TP53 mutant isogenic cell lines 1001,HCT116.p53 mutant and RKO.P53-/-was>95%efficient and the silenced cells were less invasive compared to the parental TP53 wild-type cells.AXL silencing showed a subtle trend to restore colon cancer cell sensitivity to5FU or irinotecan.Importantly,AXL expressing cells developed more invasive potential after exposure to chemotherapy compared to the AXL-silenced cells.CONCLUSION AXL is influenced by p53 status and could cause the emergence of aggressive clones after exposure to chemotherapy.These findings could have applications in cancer management. | Wael M Abdel-Rahman Noura A Al-khayyal Vidhya A Nair S R Aravind Maha Saber-Ayad | 2017 | World Journal of Gastroenterology2017，23，19： | 7 |
| 2 | Anti-inflammatory activity of Alpinia officinarum hance on rat colon inflammation and tissue damage in DSS induced acute and chronic colitis models显示文摘The purpose of this study was to investigate the possible prophylactic effects of Alpinia officinarum hance on experimentally induced acute and chronic colitis models,in-vivo and in-vitro.Acute and chronic colitis were induced in Male Wistar rats by administration of Dextran Sulfate Sodium(DSS)in drinking water.DSS induction exhibited colon shrinkage,increased the Disease Activity Index(DAI)score,increased the levels of inflammatory markers and caused severe anemia.DSS induced animals,co-treated with the hexane extract of Alpinia officinarum(HEAO)(200 mg/kg body wt),effectively suppressed colonic injury that was evidenced by the reduced DAI score,colon weight/length ratio,histological damage,proinflammatory markers and MPO activity.Further,it restored the colonic antioxidants near to normal levels by regulating the oxidative stress via attenuation of lipid peroxidation.Our results revealed that the degree of colitis caused by the administration of DSS was significantly attenuated by HEAO.In addition,the in-vitro study showed that HEAO treatment inhibited the proliferation of HT-29 cells and down regulated the mRNA expression of NF-B and COX-2.Taken together,these results suggest that HEAO is a promising anti-oxidant and anti-inflammatory agent that support its possible therapeutic role in the treatment of colitis. | Vijayabharathi Rajendiran Vidhya Natarajan Sivasithamparam Niranjali Devaraj | 2018 | Food Science and Human Wellness2018，7，4： | 5 |
| 3 | In vitro biofilm formation by uropathogenic Escherichia coli and their antimicrobial susceptibility pattern显示文摘Objective:To detect in vitro biofilm formation of uropathogenic Escherichia coli(E.coli)(UPEC) strains isolated from urine specimens and also to determine their antimicrobial susceptibility pattern using 13 commonly used antibiotics.Methods:The present study comprised of 166 urine specimens collected from tertiary care hospitals in and around Coimbatore.South India. All the specimens were subjected to gram staining,bacterial culture and the E.coli strains were screened for biofilm formation using Tube Method(TM),Congo Red Agar(CRA) and Tissue Culture Plate method(TCP) respectively.Subsequently,the antimicrobial susceptibility test was performed by Kirby Bauer-disk diffusion method for the biofilm and non-biofilm producing E. coli strains.Results:Of the 100(60.2%) E.coli strains,72 strains displayed a biofilm positive phenotype under the optimized conditions in the Tube Method and the strains were classified as highly positive(17,23.6%),moderate positive(19.26.3%) and weakly positive(36.50.0%). similarly under the optimized conditions on Congo Red agar medium,biofilm positive phenotype strains were classified as highly positive(23,23%).moderate positive(37.37%) and weakly positive(40,40%).While in TCP method,the biofilm positive phenotype strains were also classified as highly positive(6.6%),moderate positive(80.80%) and weakly positive(14,14%),it didn’t not correlate well with the tube method for detecting biofilm formation in E.coli.The rates of antibiotic resistance of biofilm producing E.coli were found to be 100%for chloramphenicol and amoxyclav(amoxicillin and clavulanic acid),86%for gentamicin and cefotaxime.84%for ceftazidime,83%for cotrimoxazole and piperacillin/tazobactam,75%for tetracycline and 70% for amikacin,Conclusions:This study reveals the prevalence and antimicrobial susceptibility pattern of biofilm and non-biofilm producing uropathogenic E.coli strains. | Poovendran Ponnusamy Vidhya Natarajan Murugan Sevanan | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，3： | 5 |
| 4 | Dog10K: an international sequencing effort to advance studies of canine domestication, phenotypes and health显示文摘Dogs are the most phenotypically diverse mammalian species, and they possess more known heritable disorders than any other non-human mammal. Efforts to catalog and characterize genetic variation across well-chosen populations of canines are necessary to advance our understanding of their evolutionary history and genetic architecture. To date, no organized effort has been undertaken to sequence the world’s canid populations. The Dog10 K Consortium(http://gffzz8e6b994fb05d4249hfxxkc0fxpcx66pkv.ffgz.tsg.suse.edu.cn kgenomes.org) is an international collaboration of researchers from across the globe who will generate 20× whole genomes from 10 000 canids in 5 years.This effort will capture the genetic diversity that underlies the phenotypic and geographical variability of modern canids worldwide. Breeds, village dogs, niche populations and extended pedigrees are currently being sequenced, and de novo assemblies of multiple canids are being constructed. This unprecedented dataset will address the genetic underpinnings of domestication, breed formation, aging, behavior and morphological variation. More generally, this effort will advance our understanding of human and canine health. | Elaine A.Ostrander Guo-Dong Wang Greger Larson Bridgett M.vonHoldt Brian W.Davis Vidhya Jagannathan Christophe Hitte Robert K.Wayne Ya-Ping Zhang the Dog10K Consortium | 2019 | National Science Review2019，6，4： | 3 |
| 5 | Emerging role of caldesmon in cancer:A potential biomarker for colorectal cancer and other cancers显示文摘Colorectal cancer(CRC) is a devastating disease, mainly because of metastasis. As a result, there is a need to better understand the molecular basis of invasion and metastasis and to identify new biomarkers and therapeutic targets to aid in managing these tumors. The actin cytoskeleton and actin-binding proteins are known to play an important role in the process of cancer metastasis because they control and execute essential steps in cell motility and contractility as well as cell division. Caldesmon(CaD) is an actin-binding protein encoded by the CALD1 gene as multiple transcripts that mainly encode two protein isoforms: High-molecular-weight CaD, expressed in smooth muscle, and low-molecular weight CaD(l-CaD), expressed in nonsmooth muscle cells. According to our comprehensive review of the literature, CaD, particularly l-CaD, plays a key role in the development, metastasis, and resistance to chemoradiotherapy in colorectal, breast, and urinary bladder cancers and gliomas, among other malignancies. CaD is involved in many aspects of the carcinogenic hallmarks, including epithelial mesenchymal transition via transforming growth factor-beta signaling, angiogenesis, resistance to hormonal therapy, and immune evasion. Recent data show that CaD is expressed in tumor cells as well as in stromal cells, such as cancerassociated fibroblasts, where it modulates the tumor microenvironment to favor the tumor. Interestingly, CaD undergoes selective tumor-specific splicing, and the resulting isoforms are generally not expressed in normal tissues, making these transcripts ideal targets for drug design. In this review, we will analyze these features of CaD with a focus on CRC and show how the currently available data qualify CaD as a potential candidate for targeted therapy in addition to its role in the diagnosis and prognosis of cancer. | Alya R Alnuaimi Vidhya A Nair Lara J Bou Malhab Eman Abu-Gharbieh Anu Vinod Ranade Gianfranco Pintus Mohamad Hamad Hauke Busch Jutta Kirfel Rifat Hamoudi Wael M Abdel-Rahman | 2022 | World Journal of Gastrointestinal Oncology2022，14，9： | 2 |
| 6 | Calponin 3 promotes invasion and drug resistance of colon cancer cells显示文摘BACKGROUND Calponin 3(CNN3)is an actin-binding protein expressed in smooth muscle and non-smooth muscle cells.It is required for cytoskeletal rearrangement and wound healing.AIM To dissect the role of CNN3 in carcinogenesis with a focus on colon cancer.METHODS A total of 20 cancer cell lines(8 breast,11 colon,and HeLa cervical cancer cell as a positive control for mesenchymal phenotype)and 57 formalin-fixed,paraffinembedded sections from archived sporadic colorectal carcinomas were included in this study.CNN3 expression analysis by western blot or immunohistochemistry was followed by functional analyses.The CNN3 gene was silenced by specific small interfering RNA(commonly known as siRNA),followed by confirmation of the silencing efficiency by western blotting.Then,the silenced cells and control siRNA-transfected cells were analyzed for changes in epithelial and mesenchymal markers,invasion,and response to 5-fluoruracil treatment.We also performed proteomics analysis using a phospho-kinase array-based panel of 45 proteins.RESULTS CNN3 showed positive expression in 6/8 breast and 9/11 colon cancer lines and in HeLa cells.Interestingly,the colorectal adenocarcinoma line SW480 was negative,while the cell line developed from its matching lymph node metastasis(SW620)was positive for CNN3.CNN3 expression was fairly consistent with the metastatic phenotype in colon cancer because it was absent in one other colon cell line from a primary site and expressed in all others.We selected SW620 for subsequent functional analyses.CNN3-silenced SW620 cells showed a reduction in collagen invasion and loss of mesenchymal markers.CNN3 silencing caused an increase in the SW620 colon cancer cell sensitivity to 5-fluorouracil.Phosphokinase array-based proteomics analysis showed that CNN3 silencing in SW620 reduced extracellular signal-regulated kinase,β-Catenin,mutant p53,c-Jun,and heat shock protein 60 activities but increased that of checkpoint kinase 2.CNN3 was expressed in 20/57(35%)colon cancer cases as shown by immunohistochemistry.CNN3 was associated with a decrease in overall survival in colon cancer in silico.CONCLUSION These results show the involvement of CNN3 in lymph node metastasis and resistance to chemotherapy in colon cancer and suggest that significant oncogenic pathways are involved in these CNN3-related actions. | Vidhya A Nair Noura A Al-khayyal Sivaramakrishnan Sivaperumal Wael M Abdel-Rahman | 2019 | World Journal of Gastrointestinal Oncology2019，11，11： | 2 |
| 7 | Sol-gel derived cobalt doped nano-titania photocatalytic system for solar light induced degradation of crystal vi- olet显示文摘 | PREETHI T ABARNA B VIDHYA K N | 2014 | Ceramics International2014，40，13： | 1 |
| 8 | Hypoglycemic effect of Mucuna pruiens seed extract on normal and streptotocin-diabetic rats显示文摘 | Bhaskar A Vidhya V G Ramya M | 2008 | Fitoterapia2008，79，： | 1 |
| 9 | Syn- thesis and characterization of epoxy-silicone-polythio- phene interpenetrating polymer network for corrosion protection of steel显示文摘 | PALRAJ S SELVARAJ M VIDHYA M | 2012 | Prog Org Coat2012，75，4： | 1 |
| 10 | Influence of substrate temperature on the materials properties of reactive DC magnetron sputtered Ti/TiN multilayered thin films显示文摘 | B. Subramanian R. Ananthakumar V.S. Vidhya M. Jayachandran | 2010 | Materials Science & Engineering B2010，，1： | 1 |
| 11 | Modeling the influence of task on attention显示文摘 | Vidhya Navalpakkam Laurent Itti | 2004 | Vision Research2004，，2： | 1 |
| 12 | An efficient process for production and purification of hyaluronic acid from Streptococcus equi subsp. zooepidemicus显示文摘 | Vidhya Rangaswamy Dharmendra Jain | 2008 | Biotechnology Letters2008，，3： | 1 |
| 13 | An efficient process for production and purification of hyaluronic acid from Streptococcus equi subsp, zooepidemicus显示文摘 | Vidhya Rangaswamy Dharmendra Jain | 2008 | Biotechnol Lett2008，30，： | 1 |
| 14 | Extraction of Nanostructured Metal Oxides from the Furnace Dust for the Realization of Low Cost Solar Applications显示文摘 | T.C. Sabari Girisun C. Babeela V. Vidhya | 2013 | Physics Procedia2013，，： | 1 |
| 15 | Human papilloma virus and oral infections:an update显示文摘 | Kumaraswamy KL Vidhya M | 2011 | J Cancer Res Ther2011，7，2： | 1 |
| 16 | Tuning of PEM fuel cell model parameters for prediction of steady state and dynamic performance under various operating conditions显示文摘 | K. Latha S. Vidhya B. Umamaheswari N. Rajalakshmi K.S. Dhathathreyan | 2012 | International Journal of Hydrogen Energy2012，，： | 1 |
| 17 | Etiology and Treatment of Hypogonadism in Adolescents显示文摘 | Vidhya Viswanathan Erica A. Eugster | 2011 | The Pediatric Clinics of North America2011，，5： | 1 |
| 18 | Materials Science and Engineering B显示文摘 | Vidhya B | 2010 | 174(1-3): 2162010，174，13： | 1 |
| 19 | Prevalence of non-diabetic renal disease in type2diabetic patients in a diabetes centre in Southern India显示文摘 | Premalatha G Vidhya K Deepa R | 2002 | J Assoc Physicians India2002，50，： | 1 |
| 20 | Evaluation of patient age as a risk factor for intraocular pressure elevation after intravitreal triamcinolone显示文摘 | Shulda D Vidhya N Prasad NM Mahalakshmi R Kolluru C Krishnadas R | 2007 | Am J Ophthalmol2007，144，3： | 1 |
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