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| 1 | Establishment of a novel corneal endothelial cell line from domestic rabbit,Oryctolagus curiculus显示文摘To develop a rabbit corneal endothelial (RCE) cell line, in vitro culture of RCE cells was initiated from Oryctolagus curiculus corneas and a novel RCE cell line was established in this study. To initiate the primary culture of RCE cells, corneas from rabbit eyes were sliced and attached into glutin-coated wells with endothelial cell surface down. After being cultured at a time-gradient interval from 48 to 6 h, the corneal slices were detached and reattached into new wells, respectively. Cells in the wells containing only a pure population of RCE cells were collected and cultured in 20% FBS-DMEM/F12 medium con- taining chondroitin sulfate, ocular extract, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), carboxymethyl-chitosan, N-acetylglucosamine hydrochloride, glucosamine hydrochloride, culture medium of rabbit corneal stromal cells and oxidation-degradation products of chondroitin sul- fate at 37℃, 5% CO2. The cultured RCE cells, in quadrangle and polygonal shapes, proliferated to con- fluence 3 weeks later. During the subsequent subculture, the shape of RCE cells changed gradually from polygonal to more fibroblastic. A novel RCE cell line, growing at a steady rate, with a population doubling time of 53.8 h, has been established and subcultured to passage 67. Chromosome analysis showed that the RCE cells exhibited chromosomal aneuploidy with the modal chromosome number of 44. The results of immuno-cytochemical staining with neuron specific enolase (NSE) confirmed that the RCE cells were in neuroectodermal origin. Combined with the results of vascular endothelial growth factor (VEGF) treatment and endothelial cell morphology recovery, it can be concluded that the cell line established here is an RCE cell line. This RCE cell line may serve as a useful tool in theoretical re- searches of mammalian corneal endothelial cells, and may also have potential application in artificial corneal endothelium development. | FAN TingJun, ZHAO Jun, FU YongFeng, CONG RiShan, GUO RuiChao, LIU WanShun, HAN BaoQin, YU QiuTao & WANG JingCollege of Marine Life Sciences, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China | 2007 | Science China(Life Sciences)2007，50，2： | 15 |
| 2 | Inhibitor of apoptosis proteins and apoptosis显示文摘Apoptosis 是在多细胞的动物的开发,动态平衡,和有免疫力的防卫起一个关键作用的一个生理的细胞死亡过程。apoptosis 蛋白质(国际机场) 的禁止者组成在一个和三次 baculovirus 国际机场重复之间拥有的蛋白质的一个家庭。他们中的一些也有一个确实有趣的新基因手指领域,并且能由有约束力、禁止的活跃 caspases 阻止房间死亡,但是被国际机场对手调整。一些证据也显示那个国际机场能调制房间周期和信号 transduction。三个主要因素,国际机场,国际机场对手,和 caspases,涉及在许多种类调整 apoptosis 的进步。许多研究和假设集中于在这三个主要因素之间的弯曲相互作用探索他们的真正的功能的模型以便开发潜在的 anticancer 药。在这评论,我们描述国际机场的分类,分子的结构,和性质并且讨论 apoptosis 的机制。我们也在恶意的诊断和治疗讨论国际机场的临床的应用程序的有希望的意义。 | Yunbo Wei Tingjun Fan Miaomiao Yu | 2008 | Acta Biochimica et Biophysica Sinica2008，40，4： | 14 |
| 3 | Development of an Inactivated Iridovirus Vaccine Against Turbot Viral Reddish Body Syndrome显示文摘Turbot(Scophthalmus maximus L.) reddish body iridovirus(TRBIV) was propagated in turbot fin cells(TF cells) and inactivated as the TRBIV vaccine with its protection efficiency evaluated in this study.TF cells were cultured in 10% bovine calf serum(BCS)-containing MEM medium(pH7.0) at 22℃,in which TRBIV propagated to a titer as high as 105.6 TCID50 mL-1.The TRBIV was inactivated with 0.1% formalin and formulated with 0.5% aluminum hydroxide.The inactivated vaccine caused neither cytopathogenic effect(CPE) on TF cells nor pathogenic effect on turbots.After being administered with the vaccine twice via muscle injection,the turbot developed high-tittered TRBIV neutralizing antibodies in a dose-dependent manner.The vaccine protected the turbot from dying with an immunoprotection rate of 83.3% as was determined via subcutaneous vaccination in the laboratory and 90.5% via bath vaccination in turbot farms,respectively.The inactivated vaccine was very immunogenic,efficiently preventing tur-bot from death.It holds the potential of being applied in aquaculture. | FAN Tingjun HU Xiuzhong WANG Liyan GENG Xiaofen JIANG Guojian YANG Xiuxia YU Miaomiao | 2012 | Journal of Ocean University of China2012，11，1： | 5 |
| 4 | Establishment and Characterization of a New Cell Line from the Kidney of Spotted Halibut Verasper variegates显示文摘A cell line,SHK,was derived from the kidney of spotted halibut Verasper variegates.The cell line was subculturedmore than 40 passages in minimum essential medium(MEM)supplemented with fetal bovine serum(FBS)and 10 ng ml-1basic fi-broblast growth factor(bFGF).Cell morphology from primary culture and subculture was observed continuously by microscopy.TheSHK cell line consisted predominantly of fibroblast-like cells.The cell line was able to grow between 20℃and 30℃with the optimum growth at 24℃and with a reduced growth between 12℃and 20℃.The growth rate of the cells increased as the proportion ofFBS increased from 10%to 20%at 28℃with optimum growth at the concentration of 20%.The doubling time of the cells was de-termined to be 44.8 h.Chromosome analysis revealed that 52%of the SHK cells maintained a normal diploid chromosome number(2n=46).The cells were successfully transfected with green fluorescent protein(GFP)reporter plasmids and the expression of GFPgene in the cells indicated the possible utility of the cells in gene expression studies.The cells were infected by lymphosystis diseasevirus(LCDV)and found to be susceptible to the virus in cytopathic effect(CPE)observation.The infection was confirmed by PCRand electron microscopy experiments,which proved the existence of the viral particles in the cytoplasm of the virus-infected cells. | WANG Xianli CHEN Songlin SHA Zhenxia FAN Tingjun WANG Na | 2010 | Journal of Ocean University of China2010，9，2： | 4 |
| 5 | Purification and characterization of phenoloxidase from Octopus ocellatus显示文摘从章鱼 ocellatus 的墨水囊的 Phenoloxidase (PO ) 被胶化过滤和离子交换层析净化,并且由把 L-dihydroxyphenylalanine (L-DOPA ) 用作特定的底层以它的生物化学、酶的性质描绘了。它从 O 被发现那 prophenoloxidase。当 153.8 kDa 的 heterodimeric 蛋白质,和 75.6 和 73.0 kDa 的二个子单元经常在 SDS 激活以后在准备被检测, ocellatus 被孤立。象 PO 一样活动显示出 7.0 的最佳的 pH, 40 的最佳的温度 ?????? | Tingjun Fan Mingyu Li Jing Wang Lingling Yang Rishan Cong | 2009 | Acta Biochimica et Biophysica Sinica2009，41，10： | 3 |
| 6 | Establishment of a Spleen Cell Line from Large Yellow Croaker Pseudosciaena crocea and its Primitive Application in Foreign Gene Transfection显示文摘A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco's modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies. | SUN Ai FAN Tingjun YANG Xiuxia XU Bin XU Xiaohui GUO Xueyang | 2011 | Journal of Ocean University of China2011，10，4： | 3 |
| 7 | Observed permafrost thawing and disappearance near the altitudinal limit of permafrost in the Qilian Mountains显示文摘Permafrost degradation has been widely reported on the Tibetan Plateau(TP).However,directly observed evidence of permafrost thawing processes and degradation rates are very limited,although it is expected to be prevalent near the periphery of a permafrost area.Here,we report permafrost changes and disappearance in the Qilian Mountains(northeastern TP)based on three boreholes instrumented along a 100 m transect during 2014–2021.Our results show that permafrost has significantly degraded in the study area:the mean downward thawing rate from the permafrost table was about 0.16 m per year while the mean upward thawing rate from the permafrost base was about 0.23 m per year.We estimate the mean lateral degradation rate of permafrost in this area was∼4.14 m per year.More dramatically,the 1.5 m thick permafrost layer at one of the boreholes thawed completely between April of 2018 and December of 2019.Our results indicate that changes in climatic condition may have played only a limited role in controlling the active layer thickness in the vicinity of the altitudinal limit of permafrost;moisture content and soil conditions play key roles in site-specific permafrost thawing.This study provides new quantitative insights for understanding changes near the altitudinal limit of permafrost,and we suggest that land surface models or Earth system model studies of the lateral heat exchanges should be implemented in order to better represent permafrost thawing processes. | Wen SUN Tingjun ZHANG Gary D.CLOW Yan-Hua SUN Wen-Yu ZHAO Ben-Ben LIANG Cheng-Yan FAN Xiao-Qing PENG Bin CAO | 2022 | Advances in Climate Change Research2022，13，5： | 2 |
| 8 | Purification and characterization of hatching enzyme from brine shrimp Artemia salina显示文摘由把 Artemia 卵壳用作特定的底层,从 Artemia 盐咸滩(AHE ) 的孵化的酶被胶化过滤和离子交换层析净化,并且在这研究生物化学地并且酶地描绘了。AHE 由钠 dodecyl sulfatepolyacrylamide 胶化电气泳动有 82.2 kDa 的分子的重量,这被发现,并且经常在准备包含了 73.3 个 kDa 分子。AHE 举办了明显的 choriolytic 活动,哪个在 pH 是最佳的 7.0 并且 40 的温度 ?? 琠慲獮畤瑣潩 ???????????????????? ??????? ?? 僚吗?? | Tingjun Fan Jing Wang Wenpeng Yuan Qiwang Zhong Ying Shi Rishan Cong | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 2 |
| 9 | Purification and characterization of phenoloxidase from brine shrimp Artemia sinica显示文摘从 Artemia sinica (AsPO ) 的 Phenoloxidase 被 Superdex 200 胶化过滤和 Q Sepharose 快流动离子交换层析净化,并且它的性质被把 L-dihydroxyphenylalanine (L-DOPA ) 用作特定的底层生物化学地并且酶地描绘。结果证明 AsPO 在分子的质量中作为 125.5 kDa 的 monomeric 蛋白质被孤立。最佳的 pH 价值和温度是 7.0 并且 50uronidase ) GhHyPRP4 倡导者驾驶的基因明确地在转基因的 Arabidopsis thaliana 的叶子和子叶被表示。而且,在叶子的 GUS 表示被冷应力显著地导致。在酵母(Schizosaccharomyces pombe ) 的 GhHyPRP4 的 Overexpression 显著地没有乙醇在食物上在 20d 消费下面在处理之上提高了房间幸存率;当食物包含了 0.01, 0.3,或 0.5% 时,他们没改变消费乙醇;但是显著地?? | Tingjun Fan Zhao Jing Xianyuan Fan Miaomiao Yu Guojian Jiang | 2011 | Acta Biochimica et Biophysica Sinica2011，43，9： | 2 |
| 10 | Establishment and Characterization of a New Marine Fish Cell Line from Ovary of Barfin Flounder(Verasper moseri)显示文摘A novel continuous ovary cell line from barfin flounder(Verasper moseri)(BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22℃ in Dulbecco's modified Eagle medium/F12 medium(DMEM/F12, 1:1; p H 7.2) supplemented with 20% fetal bovine serum(FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor(b FGF) and insulin-like growth factor-I(IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22℃. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number(46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein(GFP) positively expressed in these cells after being transformed with pc DNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish. | XU Xiaohui FAN Tingjun JIANG Guojian YANG Xiuxia | 2015 | Journal of Ocean University of China2015，14，6： | 2 |
| 11 | Cloning and Expression of lipP, A Gene Encoding a Cold-Adapted Lipase from Moritella sp.2-5-10-1显示文摘 | Xiuxia Yang Xuezheng Lin Tingjun Fan Ji Bian Xiaohang Huang | 2008 | Current Microbiology2008，，2： | 1 |
| 12 | Automatic Chinese Seal identification 显示文摘 | Fan Tingjun Tsai Wenhsiang | 1984 | Computer Vision Graphics and Images Processing1984，25，3： | 1 |
| 13 | Cloning and Expression of lipP, A Gene Encoding a Cold-Adapted Lipase from Moritella sp.2-5-10-1显示文摘 | Xiuxia Yang Xuezheng Lin Tingjun Fan Ji Bian Xiaohang Huang | 2008 | Current Microbiology2008，，2： | 1 |
| 14 | Recognizing 3-D objects using surface description显示文摘 | FAN Tingjun MEDIONI G NEVATIA R | 1989 | IEEE Transactions on Pattern Analysis Machine Intelligence1989，11，11： | 1 |
| 15 | Identification and characterization of a hemocyanin-derived phenoloxidase from the crab Charybdis japonica显示文摘 | Tingjun Fan Yanan Zhang Lingling Yang Xiuxia Yang Guojian Jiang Miaomiao Yu Rishan Cong | 2008 | Comparative Biochemistry and Physiology Part B2008，，2： | 1 |
| 16 | Preparation and characterization of polar polymeric adsorbents with high surface area for the removal of phenol from water显示文摘 | Xiaowei Zeng Tingjun Yu Peng Wang Ronghua Yuan Qing Wen Yunge Fan Chunhong Wang Rongfu Shi | 2009 | Journal of Hazardous Materials2009，，1： | 1 |
| 17 | Effects of Several Immunostimulants on Phenoloxidase and Hemocytes of the Crab Charybdis japonica显示文摘To investigate the stimulating effects of immunostimulants on the autogenous immunocompetence of crabs and the possible mechanisms involved, the immunostimulating effects of β-1,3-glucan, lipopolysaccharide (LPS), inactivated Vibrio harveyi and Vibrio anguillarum on phenoloxidase (PO) and hemocytes of Charybdis japonica were investigated in this study. It was found that the yields and the enzymatic activities of purified PO in C. japonica increased significantly after the crabs were treated with immunostimulants, while the unit enzymatic activities remained almost the same. After treatment with β-1,3-glucan and LPS, the amount of rough endoplasmic reticulum (RER) and the number of mitochondria in both semigranular cells and granular cells increased greatly, and the number of cytoplasmic granules decreased but with enlarged volume. However, the corresponding characteristics of hyaline cells remained almost the same. On the other hand, the number of granules in semigranular cells decreased greatly, and the number of mitochondria of hyaline cells increased greatly, after treatment with inactivated vibrios. It may be concluded that the effect of polysaccharide immunostimulants on the innate immune system of C. japonica is different from that of inactivated vibrio immu-nostimulants. The immunity-enhancing mechanism of polysaccharides in crab autogenous immunocompetence is probably accomplished by the increased yields of PO and total PO activities, while that of inactivated vibrios is probably accomplished by the partially increased yields of PO and total PO activities as well as the significantly improved phagocytotic abilities of semigranular cells and hyaline cells. | FAN Tingjun YU Miaomiao YANG Lingling SHI Zhenping SUN Wenjie CONG Rishan YANG Xiuxia JIANG Guojian | 2009 | Journal of Ocean University of China2009，8，3： | 0 |
| 18 | Degradation properties of fulvic acid and its microbially driven mechanism from a partial nitritation bioreactor through multi-spectral and bioinformatic analysis显示文摘This study employed multispectral techniques to evaluate fulvic acid(FA)compositional characteristic and elucidate its biodegradation mechanisms during partial nitritation(PN)process.Results showed that FA removal efficiency(FRE)decreased from 90.22 to 23.11%when FA concentrations in the reactor were increased from 0 to 162.30 mg/L,and that molecular size,degree of aromatization and humification of the effluent FA macromolecules all increased after treatment.Microbial population analysis indicated that the proliferation of the Comamonas,OLB12 and Thauera exhibit high FA utilization capacity in lower concentrations(<50.59 mg/L),promoting the degradation and removal of macromolecular FA.In addition,the sustained increase in external FA may decrease the abundance of above functional microorganisms,resulting in a rapid drop in FRE.Furthermore,from the genetic perspective,the elevated FA levels restricted carbohydrate(ko00620,ko00010 and ko00020)and nitrogen(HAO,AMO,NIR and NOR)metabolism-related pathways,thereby impeding FA removal and total nitrogen loss associated with N_(2)O emissions. | Quanhao Dou Li Zhang Tingjun Dong Zixuan Song Xuepeng Fan Yongzhen Peng Xiayan Wang Jiachun Yang | 2024 | Journal of Environmental Sciences2024，，1： | 0 |
| 19 | Development of 57 Novel Polymorphic Microsatellite Markers in Half-Smooth Tongue Sole(Cynoglossus semilaevis)显示文摘Half-smooth tongue sole (Cynoglossus semilaevis) is a promising species for aquaculture in China.The wild population of C. semilaevis is under threat from environmental factors. Microsatellite markers are very suitable for assessing genetic diversity. Four microsatellite-enriched libraries of half smooth tongue sole (Cynoglossus semilaevis) were constructed,from which 57 polymorphic microsatellites were isolated and characterized.The polymorphism of these microsatellites was assessed by genotyping in 30 individual fish.The number of alleles ranged from 2 to 11, with an average of 4.614 alleles per locus.The values of observed and expected heterozygosities ranged from 0.1000 to 1.0000 and from 0.0966 to 0.8847 respectively. Polymorphism information content (PIC) ranged from 0.0905 to 0.862.These markers would be useful for population structure assessment,genetic linkage map construction and parentage analysis for this species. | MIAO Guidong XU Ying WANG Di CHEN Songlin FAN Tingjun TIAN Yongsheng | 2011 | Journal of Ocean University of China2011，10，3： | 0 |
| 20 | Patterns and Cellular Mechanisms of Arm Regeneration in Adult Starfish Asterias rollestoni Bell显示文摘To understand the mechanisms of starfish regeneration, the arms of adult starfish Asterias rollestoni Bell were amputated and their regeneration patterns and cellular mechanisms were studied.It was found that cells in the outer epidermis and inner parietal peritoneum near the end of the stump began to dedifferentiate 4 d after amputation. The dedifferentiated cells in the outer epidermis proliferated,migrated to the wound site and formed a thickened pre-epidermis which would then re-differentiate gradually into ma-ture epidermis.The new parietal peritoneum formed on the coelomic side of wound might be from the curvely elongated parietal peritoneum, resulting from the dedifferentiated and proliferated cells by extension. Afterwards, the proliferated cells made the outer epidermis and inner parietal peritoneum invaginate into the interior dermis and formed blastema-like structures together with induced dedifferentiated dermal cells. Most interestingly, the arm regeneration in A. rollestoni was achieved synchronously by de novo arm-bud formation and growth, and arm-stump elongation. The crucial aspects of arm-bud formation included cell dedifferentiation, proliferation and migration, while those of arm-stump elongation included cell dedifferentiation, proliferation, invagination, and arm-wall-across blastema-like structure formation. The unique pattern and cellular mechanisms of amputated arm regeneration make it easier to understand the rapid regeneration process of adult starfish. This study may lay solid foundations for the research into mo-lecular mechanisms of echinoderm regeneration. | FAN Tingjun FAN Xianyuan DU Yutang SUN Wenjie ZHANG Shaofeng LI Jiaxin | 2011 | Journal of Ocean University of China2011，10，3： | 0 |
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