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| 1 | 罗非鱼肾脏细胞系的建立及其生物学特性显示文摘采用组织块移植法,对尼罗罗非鱼(Oreochromis niloticus)的肾脏组织细胞进行原代培养,建立了罗非鱼肾脏细胞系,已稳定传代培养50代以上,命名为TiK。罗非鱼肾脏细胞系为纤维样细胞,其最佳培养基为DMEM,最适培养温度为28℃,最适血清浓度为15%。在最适培养条件下,罗非鱼肾脏细胞系的群体倍增时间为45.8 h。细胞经液氮冷冻保存6个月后进行复苏,经台盼蓝染色,约(89.84±3.48)%的细胞具有活性,复苏后细胞生长旺盛。染色体分析显示,第32代罗非鱼肾脏细胞系染色体数目分布在20~66之间,众数为48。使用本实验室分离鉴定的罗非鱼病毒感染罗非鱼肾脏细胞,可产生典型的细胞病变效应,表明罗非鱼肾脏细胞对该病毒敏感。该细胞系的建立为罗非鱼病毒病防控技术研究提供了重要的实验材料。 | 赵建青 贾鹏 刘文枝 周勇 江南 范玉顶 易梅生 曾令兵 | 2019 | 中国水产科学2019，0，2： | 9 |
| 2 | Initiation of two ovarian cell lines from Fugu rubripes(Temminck et.Schlegel)显示文摘The ovary is an excellent system for studying stem cell renewal and differentiation,which is under the control of ovarian somatic cells.In order to understand oogenesis in Fugu rubripes(Temminck et.Schlegel) as a marine fish model of aquaculture importance,we established cell lines called TSOC1 and TSOC2 from a juvenile ovary of this organism.TSOC1 is composed of spindle epithelial-like cells,while the other is cobblestone-like cells.Therefore,TSOC1 and TSOC2 appear to consist of ovarian somatic cells.Growth requirement condition was investigated including temperature,concentration of FBS and p H.Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with p EGFP-N3 vector,indicating its potential utility for genetic manipulation such as gene function studies.It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells,and most convincingly,the observation of viral particles by electron microscopy,demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells.It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis.Therefore,establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas. | ZHENG Debin ZHANG Bo SONG Wenping PAN Luqing MA Chao XIAO Guangxia | 2015 | Acta Oceanologica Sinica2015，34，10： | 0 |
| 3 | 宽口裂腹鱼尾鳍细胞系的建立及其应用显示文摘宽口裂腹鱼为塔里木河水系的特有物种。为建立其尾鳍细胞系,笔者采用组织块法和胰酶消化法相结合,分别用含胎牛血清的DME/F12培养基体外培养尾鳍组织。初步建立宽口裂腹鱼尾鳍细胞系,细胞传代培养至45代。细胞呈悬浮生长,最适培养液为DME/F12,最适胎牛血清质量分数为20%,最适温度为25℃。第10代细胞的群体倍增时间为24.94 h,细胞呈“S”型生长。第6代细胞液氮冷冻保存180 d后复苏,经台盼蓝染色计数,(88.72±0.99)%的宽口裂腹鱼尾鳍细胞系具有活性,复苏后增殖速度快,可正常传代。细菌、真菌、支原体的鉴定未发现污染。第10代细胞线粒体16S rRNA基因测序结果与GenBank中基因序列做一致性对比,宽口裂腹鱼尾鳍细胞系与NC036933.1的一致率达99.91%,从分子水平证明,宽口裂腹鱼尾鳍细胞系来自宽口裂腹鱼。NaCl质量分数为8‰时,宽口裂腹鱼尾鳍细胞系的相对增殖率最高;NaHCO3质量浓度为7 g/L时,宽口裂腹鱼尾鳍细胞系的相对增殖率最高;宽口裂腹鱼尾鳍细胞系的相对增殖率随盐碱度增加呈先升后降趋势。 | 代金彩 李丽 李学涛 魏杰 聂竹兰 | 2023 | 水产科学2023，42，1： | 0 |
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