|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Isolation and characterization of H7N9 viruses from live poultry markets--Implication of the source of current H7N9 infection in humans显示文摘On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more 'human-like'. It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health. | SHI JianZhong DENG GuoHua LIU PeiHong ZHOU JinPing GUAN LiZheng LI WenHui LI XuYong GUO Jing WANG GuoJun FAN Jun WANG JinLiang LI YuanYuan JIANG YongPing LIU LiLing TIAN GuoBin LI ChengJun CHEN HuaLan | 2013 | Chinese Science Bulletin2013，58，16： | 85 |
| 2 | Prevalence of Nontraumatic Osteonecrosis of the Femoral Head and its Associated Risk Factors in the Chinese Population: Results from a Nationally Representative Survey显示文摘 | De-Wei Zhao Mang Yu Kai Hu Wei Wang Lei Yang Ben-Jie Wang Xiao-Hong Gao Yong-Ming Guo Yong-Qing Xu Yu-Shan Wei Si-Miao Tian Fan Yang Nan Wang Shi-Bo Huang Hui Xie Xiao-Wei Wei Hai-Shen Jiang Yu-Qiang Zang Jun Ai Yuan-Liang Chen Guang-Hua Lei Yu-Jin Li Geng Tia Zong-Sheng Li Yong Cao Li Ma | 2015 | Chinese Medical Journal2015，，21： | 151 |
| 3 | RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings显示文摘在有前列腺癌症的不同赛跑的病人之中有显著不同;然而,位于这差别下面的机制仍然保持不清楚。这里,我们在场 transcriptome 的一处全面风景用 RNA-seq,越过关于基因熔化的前列腺癌症 transcriptomes 的揭示巨大的差异,长 noncoding RNA (长 ncRNA ) ,其他的拼接和体的变化从中国人口 14 主要前列腺癌症和他们的配对的正常对应物介绍。14 个肿瘤(21.4%) 中的三个在中国病人怀有 TMPRSS2 尔格熔化,和这熔化的低流行进一步在一个另外的肿瘤集合(10/54=18.5%) 被证实。尤其是,二新奇基因熔化, CTAGE5-KHDRBS3 (20/54=37%) 和 USP9Y-TTTY15 (19/54=35.2%) ,在我们的耐心的队经常发生了。介绍的进一步系统的 transcriptional 识别了是在肿瘤表示的差别的众多的长 ncRNAs。在长 ncRNA 和基因的表示之间的关联的分析建议长 ncRNAs 可以在 transcriptional 规定以外有功能。这研究在中国人口产出新卓见进前列腺癌症的致病。 | Shancheng Ren Zhiyu Peng Jian-Hua Mao Yongwei Yu Changjun Yin Xin Gao Zilian Cui Jibin Zhang Kang Yi Weidong Xu Chao Chen Fubo Wang Xinwu Guo Ji Lu Jun Yang Min Wei Zhijian Tian Yinghui Guan Liang Tang Chuanliang Xu Linhui Wang Xu Gao Wei Tian Jian Wang Huanming Yang Jun Wang Yinghao Sun | 2012 | Cell Research2012，22，5： | 66 |
| 4 | Current pattern of Chinese dialysis units: a cohort study in a representative sample of units显示文摘 | ZHOU Qiu-gen JIANG Jian-ping WU Sheng-jie TIAN Jian-wei CHEN Jiang-hua YU Xue-qing CHEN Ping-yan MEI Chang-lin XIONG Fei SHI Wei ZHOU Wei LIU Xu-sheng SUN Shi-ren XIE Di LIU Jun XU Xin HOU Fan-fan | 2012 | Chinese Medical Journal2012，，19： | 69 |
| 5 | The Genome of Dendrobium officinale Illuminates the Biology of the Important Traditional Chinese Orchid Herb显示文摘 | Liang Yan Xiao Wang Hui Liu Yang Tian Jinmin Lian Ruijuan Yang ShumeiHao Xuanjun Wang Shengchao Yang Qiye Li Shuai Qi Ling Kui Moses Okpekum Xiao Ma Jiajin Zhang Zhaoli Ding Guojie Zhang Wen Wang Yang Dong Jun Sheng | 2015 | Molecular Plant2015，8，6： | 64 |
| 6 | Persistence and clearance of viral RNA in 2019 novel coronavirus disease rehabilitation patients显示文摘Background:A patient’s infectivity is determined by the presence of the virus in different body fluids,secretions,and excreta.The persistence and clearance of viral RNA from different specimens of patients with 2019 novel coronavirus disease(COVID-19)remain unclear.This study analyzed the clearance time and factors influencing 2019 novel coronavirus(2019-nCoV)RNA in different samples from patients with COVID-19,providing further evidence to improve the management of patients during convalescence.Methods:The clinical data and laboratory test results of convalescent patients with COVID-19 who were admitted to from January 20,2020 to February 10,2020 were collected retrospectively.The reverse transcription polymerase chain reaction(RT-PCR)results for patients’oropharyngeal swab,stool,urine,and serum samples were collected and analyzed.Convalescent patients refer to recovered non-febrile patients without respiratory symptoms who had two successive(minimum 24 h sampling interval)negative RT-PCR results for viral RNA from oropharyngeal swabs.The effects of cluster of differentiation 4(CD4)+T lymphocytes,inflammatory indicators,and glucocorticoid treatment on viral nucleic acid clearance were analyzed.Results:In the 292 confirmed cases,66 patients recovered after treatment and were included in our study.In total,28(42.4%)women and 38 men(57.6%)with a median age of 44.0(34.0-62.0)years were analyzed.After in-hospital treatment,patients’inflammatory indicators decreased with improved clinical condition.The median time from the onset of symptoms to first negative RT-PCR results for oropharyngeal swabs in convalescent patients was 9.5(6.0-11.0)days.By February 10,2020,11 convalescent patients(16.7%)still tested positive for viral RNA from stool specimens and the other 55 patients’stool specimens were negative for 2019-nCoV following a median duration of 11.0(9.0-16.0)days after symptom onset.Among these 55 patients,43 had a longer duration until stool specimens were negative for viral RNA than for throat swabs,with a median delay of 2.0(1.0-4.0)days.Results for only four(6.9%)urine samples were positive for viral nucleic acid out of 58 cases;viral RNA was still present in three patients’urine specimens after throat swabs were negative.Using a multiple linear regression model(F=2.669,P=0.044,and adjusted R2=0.122),the analysis showed that the CD4+T lymphocyte count may help predict the duration of viral RNA detection in patients’stools(t=-2.699,P=0.010).The duration of viral RNA detection from oropharyngeal swabs and fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(15 days vs.8.0 days,respectively;t=2.550,P=0.013)and the duration of viral RNA detection in fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(20 days vs.11 days,respectively;t=4.631,P<0.001).There was no statistically significant difference in inflammatory indicators between patients with positive fecal viral RNA test results and those with negative results(P>0.05).Conclusions:In brief,as the clearance of viral RNA in patients’stools was delayed compared to that in oropharyngeal swabs,it is important to identify viral RNA in feces during convalescence.Because of the delayed clearance of viral RNA in the glucocorticoid treatment group,glucocorticoids are not recommended in the treatment of COVID-19,especially for mild disease.The duration of RNA detection may relate to host cell immunity. | Yun Ling Shui-Bao Xu Yi-Xiao Lin Di Tian Zhao-Qin Zhu Fa-Hui Dai Fan Wu Zhi-Gang Song Wei Huang Jun Chen Bi-Jie Hu Sheng Wang En-Qiang Mao Lei Zhu Wen-Hong Zhang Hong-Zhou Lu | 2020 | Chinese Medical Journal2020，，9： | 58 |
| 7 | Design and engineering implementation of non-supplementary fired compressed air energy storage system: TICC-500显示文摘The integration and accommodation of the wind and solar energy pose great challenges on today’s power system operation due to the intermittent nature and volatility of the wind and solar resources.High efficient large-scale electrical energy storage is one of the most effective and economical solutions to those problems.After the comprehensive review of the existing storage technologies,this paper proposes an overall design scheme for the Non-supplementary Fired Compressed Air Energy Storage(NFCAES)system,including system design,modeling and efficiency assessment,as well as protection and control.Especially,the design principles of the multistage regenerative,i.e.heat recovery system which is used to fully recycle and utilize the waste heat from compression are provided,so as the overall system efficiency evaluation method.This paper theoretically ascertains the storage decoupling rules in the potential and internal energy of molecular compressed air and reveals the conversion mechanism of gas,heat,power,electricity and other forms of energy.On this basis,a 500-k W physical simulation system of CAES system(TICC-500,Tsinghua-IPCCAS-CEPRI-CAES)is built,which passed a system-wide 420-k W load power generation test with less pollution and zero carbon emissions.Besides,the multi-form energy conversion of multi-stage regenerative CAES and storage efficiency is verified,especially its incomparable superiority in solving the uncertainty problem in wind and solar power generation.Finally,the propaganda and application scenario of the CAES system in China is introduced. | MEI Sheng Wei WANG Jun Jie TIAN Fang CHEN Lai Jun XUE Xiao Dai LU Qiang ZHOU Yuan ZHOU Xiao Xin | 2015 | Science China(Technological Sciences)2015，58，4： | 37 |
| 8 | How many sutures in the southern Central Asian Orogenic Belt:Insights from East Xinjiang-West Gansu(NW China)?显示文摘How ophiolitic mèlanges can be defined as sutures is controversial with regard to accretionary orogenesis and continental growth.The Chinese Altay,East junggar,Tianshan,and Beishan belts of the southern Central Asian Orogenic Belt(CAOB) in Northwest China,offer a special natural laboratory to resolve this puzzle.In the Chinese Altay,the Erqis unit consists of ophiolitic melanges and coherent assemblages,forming a Paleozoic accretionary complex.At least two ophiolitic melanges(Armantai,and Kelameili) in East Junggar,characterized by imbricated ophiolitic melanges,Nb-enriched basalts,adakitic rocks and volcanic rocks,belong to a Devonian-Carboniferous intra-oceanic island arc with some Paleozoic ophiolites,superimposed by Permian arc volcanism.In the Tianshan,ophiolitic melanges like Kanggurtag,North Tianshan,and South Tianshan occur as part of some Paleozoic accretionary complexes related to amalgamation of arc terranes.In the Beishan there are also several ophiolitic melanges,including the Hongshishan,Xingxingxia-Shibangjing,Hongliuhe-Xichangjing,and Liuyuan ophiolitic units.Most ophiolitic melanges in the study area are characterized by ultramafic,mafic and other components,which are juxtaposed,or even emplaced as lenses and knockers in a matrix of some coherent units.The tectonic settings of various components are different,and some adjacent units in the same melange show contrasting different tectonic settings.The formation ages of these various components are in a wide spectrum,varying from Neoproterozoic to Permian.Therefore we cannot assume that these ophiolitic melanges always form in linear sutures as a result of the closure of specific oceans.Often the ophiolitic components formed either as the substrate of intra-oceanic arcs,or were accreted as lenses or knockers in subduction-accretion complexes.Using published age and paleogeographic constraints,we propose the presence of (1) a major early Paleozoic tectonic boundary that separates the Chinese Altay-East Junggar multiple subduction systems of the southern Siberian active margin from those of the northern Tarim;and (2) a major Permian suture zone that separates the Tianshan-Beishan from the northern active margin of the Tarim Craton.These new observations and interpretations have broad implications for the architecture and crustal growth of central Asia and other ancient orogens as well. | Wenjiao Xiao Chunming Han Wei Liu Bo Wan Ji'en Zhang Songjian Ao Zhiyong Zhang Dongfang Song Zhonghua Tian Jun Luo | 2014 | Geoscience Frontiers2014，5，4： | 31 |
| 9 | The abnormal expression of retinoic acid receptor-β,p53 and Ki67 protein,n normal,premalignant and malignant esophageal tissues显示文摘AIM: Esophageal cancer remains a significant healthiproblem worldwide. It is important to investigate alterationsin expression of retinoic acid receptor-β, p53 and Ki67proteins in esophageal carcinogenesis.METHODS: To find biomarkers for early identification ofesophageal cancer, we analyzed the retinoic acid receptor-j3,p53 protein and the proliferation marker Ki67 in surgicalspecimens of normal, mildly, and severely dysplastic andmalignant esophageal tissues by in situ hybridization ofRNA and immunohistochemistry.RESULTS: RAR-β was expressed in 94.3 %(33/35) of normalmucosae, 67.8 %(19/28) of the mild, 58.1% (18/31) of thesevere ieaions and 53.2 % (116/218) of tumor samples. RAR-β mRNA was expressed in 62.7 % (42/67), 55.1% (43/78) and29.2 % (7/24) of well, moderated and poorly differentiatedSSCs. The p53 and Ki67 proteins were 5.9 % (2/34) of thenormal mucosa. P53 and Ki67 stained positively in 10.7 % (3/28) and 21.4 % (6/28) of mild dysplasia, and 51.6 %(16/31)and 58. 1% (18/31) of severely dysplasia respectively.Samples from esophageal cancer showed no higher levers ofp53 and Ki67 expression than seen in severely dysplasticlesions. There was significant difference of RAR-β、 p53 andKi67 expression between normal mucosa and dysplatictissue or esophageal cancer.CONCLUSION: Loss of RAR-β expression and acctnulation ofp53 and Ki67 proteins may serve as biomarkers for earlyidentification of esophageal cancer in the high-riskpopulations. | Min Xu Sheng-Zu Chen,Department of Nuclear Medcine,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 10002,1,China Yu-Lan Jin Jun Fu Hong Huang Ping Qu Hai-Mei Tian ZhaoYang Liu Wei Zhang,Central Laboratory for Tumor Biology,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，2： | 34 |
| 10 | Protein induced by vitamin K absence or antagonist-Ⅱ versus alpha-fetoprotein in the diagnosis of hepatocellular carcinoma: A systematic review with meta-analysis显示文摘Background: As a promising biomarker of hepatocellular carcinoma(HCC), protein induced by vitamin K absence or antagonist-Ⅱ(PIVKA-Ⅱ) has been studied extensively. However, its diagnostic capability varies across HCC studies. This study aimed to compare the performance of PIVKA-Ⅱ with alpha-fetoprotein(AFP) in the diagnosis of HCC. Data sources: A systematic literature search was conducted to identify the studies from MEDLINE, Embase and Cochrane Library Databases, which were published up to December 20, 2017 to compare the diagnostic capability of PIVKA-Ⅱ and AFP for HCC. The data were pooled using random effects model. Pooled sensitivity and specificity were calculated. Summary receiver operating characteristic curve(ROC) was employed to evaluate the diagnostic accuracy of each marker. Results: Thirty-one studies were included. The pooled sensitivity(95% CI) of PIVKA-Ⅱ and AFP was 0.66(0.65–0.68) and 0.66(0.65–0.67), respectively in diagnosis of HCC; and the corresponding pooled specificity(95% CI) was 0.89(0.88–0.90) and 0.84(0.83–0.85), respectively. The area under the ROC curve(AUC) of PIVKA-Ⅱ and AFP was 0.856(0.817–0.895) and 0.770(0.728–0.811), respectively. Subgroup analysis showed that PIVKA-Ⅱ was superior to AFP in terms of the AUC for both small HCC( < 3 cm) [0.863(0.825–0.901) vs 0.717(0.658–0.776)] and large HCC( ≥ 3 cm) [0.854(0.811–0.897) vs 0.729(0.682–0.776)]; for American [0.926(0.897–0.955) vs 0.698(0.594–0.662)], European [0.772(0.743–0.801) vs 0.628(0.594–0.662)], Asian [0.838(0.812–0.864) vs 0.785(0.764–0.806)] and African [0.812(0.794–0.840) vs 0.721(0.675–0.767)] HCC patients; and for HBV-related [0.909(0.866–0.951) vs 0.714(0.673–0.755)] and mixed-etiology [0.847(0.821–0.873) vs 0.794(0.772–0.816)] HCC. Conclusion: This meta-analysis indicates that PIVKA-Ⅱ is better than AFP in terms of the accuracy for diagnosing HCC, regardless of tumor size, patient ethnic group, or HCC etiology. | Hao Xing Yi-Jie Zheng Jun Han Han Zhang Zhen-Li Li Wan-Yee Lau Feng Shen Tian Yang | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，6： | 34 |
| 11 | The General Design and Technology Innovations of CAP1400显示文摘行波堆为一次通过式燃料循环反应堆,其利用堆芯自增殖大大降低了对浓缩和后处理的需求。自增殖将次临界换料燃料转化为新的临界燃料,从而使增殖燃烧波得以扩散。该理念建立在增殖燃烧波和燃料的相对移动的基础上。因此,燃料或增殖燃烧波相对于固定的观察器而言是移动的。行波堆最实用的体现就是能够在将核反应保持在同一位置的同时移动燃料——有时行波堆也被称为'驻波堆'。行波堆能够使用换料铀燃料运行,换料铀燃料包括完全贫化铀、天然铀和低浓缩铀燃料(即235U含量为5.5%或更低的燃料),这些燃料通常在快谱中达不到临界状态。轻水反应堆卸出的乏燃料也可以作为行波堆的换料燃料。上述情况均无需后处理即可实现极高的燃料利用率和燃料废物量的显著降低。当换料燃料为贫化铀时,行波堆的最大优势得以实现,即在启动后,无需浓缩设施,就可维持最先启动的反应堆和一连串后续的反应堆的运行。自2006年起,泰拉能源公司(Terra Power)与50多个机构高度协作,开展了概念设计、工程设计和相关技术开发活动,力争到2026年实现将第一个机组投入使用。本文总结了行波堆技术,包括它的发展计划及其进展,分析了行波堆的社会和经济效益。 | Mingguang Zheng Jinquan Yan Jun Shenttu Lin Tian Xujia Wang Zhongming Qiu | 2016 | Engineering2016，2，1： | 27 |
| 12 | The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis显示文摘Eucommia ulmoides,也叫的强壮的橡胶树,是一棵经济地重要的树;然而,它的染色体顺序的缺乏 ?限制基本生物研究和这植物种的应用研究。这里,我们在场它的 1.2-Gb 染色体的一个高质量的集会(支架 N50 ?=? 1.88 Mb ) 与至少 26 ? 为 E 的 723 预言的基因。ulmoides,顺序 Garryales 的首先定序的染色体,它用联合定序的 Illumina 的综合策略被获得,定序的 PacBio,和印射的 BioNano。作为到 lamiids 和 campanulids 的姐妹 taxon, E。ulmoides 经历了三倍由核心 eudicots 而是没有进一步整个染色体的复制分享了的一个古老的染色体 ? 在最后 1.25 亿年里。E。ulmoides 为涉及压力回答和第二等的代谢物的生合成的多重基因展出高表达式层次或基因数字扩大,它可以说明它的可观的环境适应性。与橡胶树(Hevea brasiliensis ) 相对照,它生产 cis 聚异式戊二我烯, E。ulmoides 演变综合经由 farnesyl diphosphate synthases (FPS ) 的长链的 trans 聚异式戊二我烯。而且, FPS 和橡胶延伸 factor/small 橡胶粒子蛋白质基因家庭从 H 独立地被扩展。brasiliensis 系。这些结果提供新卓见进 E. 的生物学 ? ulmoides 和聚异式戊二我烯生合成的起源。 | Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du | 2018 | Molecular Plant2018，11，3： | 24 |
| 13 | Deep carbon cycles constrained by a large-scale mantle Mg isotope anomaly in eastern China显示文摘Although deep carbon recycling plays an important role in the atmospheric CO_2 budget and climate changes through geological time,the precise mechanisms remain poorly understood.Since recycled sedimentary carbonate through plate subduction is the main light-δ^(26)Mg reservoir within deep-Earth,Mg isotope variation in mantle-derived melts provides a novel perspective when investigating deep carbon cycling.Here,we show that the Late Cretaceous and Cenozoic continental basalts from 13 regions covering the whole of eastern China have low δ^(26)Mg isotopic compositions,while the Early Cretaceous basalts from the same area and the island arc basalts from circum-Paciic subduction zones have mantle-like or heavy Mg isotopic characteristics.hus,a large-scale mantle low δ^(26)Mg anomaly in eastern China has been delineated,suggesting the contribution of sedimentary carbonates recycled into the upper mantle,but limited into the lower mantle.his large-scale spatial and temporal variation of Mg isotopes in the mantle places severe constraints on deep carbon recycling via oceanic subduction. | Shu-Guang Li Wei Yang Shah Ke Xunan Meng Hengci Tian Lijuan Xu Yongsheng He Jian Huang Xuan-Ce Wang Qunke Xia Weidong Sun Xiaoyong Yang Zhong-Yuan Ren Haiquan Wei Yongsheng Liu Fancong Meng Jun Yan | 2017 | National Science Review2017，4，1： | 23 |
| 14 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 15 | Long-term cycles in the carbon reservoir of the Quaternary ocean: a perspective from the South China Sea显示文摘In recent years,long-term,high-resolution records from the deep sea and ice-cores have ofered new research opportunities for Quaternary science.Paleoclimate studies are no longer restricted to individual glacial cycles,but extend to long-term(≥105 yr)processes across those cycles.Ocean Drilling Program Leg 184 of the South China Sea in 1999 uncovered well-preserved sediment sections,in which three long-term cycles in Pleistocene carbon isotope(δ13C)sequence have been found and demonstrated to be common in the global ocean.Subsequent discoveries conirm the existence of long-term processes of 105yr in both the hydrologic(ice-sheet changes)and carbon(biogeochemical changes)cycles,posing the question whether the two processes are related.he present review shows that the long-eccentricity cycles prevail throughout theδ13C and other biogeochemical records in geologic history,and 400-kyr cycles in the oceanicδ13C sequence before the Quaternary can be hypothetically explained by changes in ratio between particulate and dissolved organic carbon(POC/DOC)in the ocean,depending on the monsoon-controlled nutrient supply.his is a‘DOC hypothesis’.However,ocean restructuring at 1.6 Ma marked by the isolation of a sluggish abyss under the Southern Ocean has obscured the long-eccentricity 400-kyr signal in oceanicδ13C.he last million-year period has experienced two major changes in the climate regime,namely the mid-Pleistocene transition(MPT)centered at 0.9 Ma and the mid-Brunhes event(MBE)around 0.4 Ma.he MPT and MBE were preluded byδ13C maxima-III(δ13Cmax-III)~1.0 Ma andδ13Cmax-II~0.5 Ma,respectively.Together with similar hydroclimatic phenomena over corresponding glacial cycles,the two groups of hydrologic and biogeochemical events appear to have been driven largely by oceanographic changes in the Southern Ocean.herefore,we interpret that the long-term biogeochemical processes originating from the Southern Ocean must have played a crucial role in Quaternary ice-sheet waxing and waning. | PinXian Wang QianYu Li Jun Tian ZhiMin Jian ChuanLian Liu Li Li WenTao Ma | 2014 | National Science Review2014，1，1： | 23 |
| 16 | Casticin-induced apoptosis involves death receptor 5 upregulation in hepatocellular carcinoma cells显示文摘AIM:To investigate the apoptotic activities of casticin in hepatocellular carcinoma(HCC) cells and its molecular mechanisms.METHODS:PLC/PRF/5 and Hep G2 cell lines were cultured in vitro and the inhibitory effect of casticin on the growth of cells was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolim bromide(MTT) assay.The apoptotic cell death was examined using the cell apoptosis enzyme linked immunosorbent assay(ELISA) detection kit,flow cytometry(FCM) after propidium iodide(PI) staining and DNA agarose gel electrophoresis.The caspase activities were measured using ELISA.Reactive oxygen species(ROS) production was evaluated by FCM after dichlorodihydrofluorescein diacetate(DCFH-DA) probe labeling.Intracellular glutathione(GSH) content was measured using a glutathione assay kit.The expression of death receptor(DR)4 and DR5 proteins was analyzed by Western blotting and FCM.RESULTS:Casticin significantly inhibited the growth of human HCC(PLC/PRF/5 and Hep G2) cells in a dosedependent manner(P < 0.05).Casticin increased the percentage of the sub-G1 population in HCC cells in a concentration-dependent manner.The potency of casticin to PLC/PRF/5 cells was higher than that of 5-flurouracil(26.8% ± 4.8% vs 17.4% ± 5.1%) at 10 μmol/L for 24 h.Casticin increased the levels of Histone/DNA fragmentation and the levels of active caspase-3,-8 and-9 in a concentration-dependent manner(P < 0.05).Treatment with 30 μmol/L casticin for 24 h resulted in the formation of a DNA ladder.Casticin reduced the GSH content(P < 0.05),but did not affect the level of intracellular ROS in PLC/PRF/5 and Hep G2 cells.The thiol antioxidants,acetylcysteine(NAC) and GSH restored GSH content and attenuated casticin-induced apoptosis.In contrast,the nonthiol antioxidants,butylated hydroxyanisole and mannitol failed to do so.In the HCC cells treated with casticin for 24 h,DR5 protein level was increased.The expression of DR5 protein induced by casticin was inhibited by NAC.Pretreatment with DR5/Fc chimera protein,a blocking antibody,effectively attenuated the induction of apoptosis by casticin.CONCLUSION:Casticin-induced apoptosis of HCC cells is involved in GSH depletion and DR5 upregulation. | Jun Yang Yun Yang Li Tian Xi-Feng Sheng Fei Liu Jian-Guo Cao | 2011 | World Journal of Gastroenterology2011，17，38： | 21 |
| 17 | Diffuse axonal injury after traumatic cerebral microbleeds: an evaluation of imaging techniques显示文摘Previous neuropathological studies regarding traumatic brain injury have primarily focused on changes in large structures, for example, the clinical prognosis after cerebral contusion, intracerebral hematoma, and epidural and subdural hematoma. In fact, many smaller injuries can also lead to severe neurological disorders. For example, cerebral microbleeds result in the dysfunction of adjacent neurons and the disassociation between cortex and subcortical structures. These tiny changes cannot be adequately visualized on CT or conventional MRI. In contrast, gradient echo sequence-based susceptibility-weighted imaging is very sensitive to blood metabolites and microbleeds, and can be used to evaluate traumatic cerebral microbleeds with high sensitivity and accuracy. Cerebral microbleed can be considered as an important imaging marker for diffuse axonal injury with potential relevance for prognosis. For this reason, based on experimental and clinical studies, this study reviews the role of imaging data showing traumatic cerebral microbleeds in the evaluation of cerebral neuronal injury and neurofunctional loss. | Jun Liu Zhifeng Kou Yongquan Tian | 2014 | Neural Regeneration Research2014，9，12： | 21 |
| 18 | Curative effect of heat-sensitive moxibustion on chronic persistent asthma: a multicenter randomized controlled trial显示文摘OBJECTIVE:To compare the curative effects of heat-sensitive moxibustion with conventional drugs on chronic persistent asthma and seek a valuable therapy to replaceWestern Medicine.METHODS:The participants in this multi-center,randomized,and controlled study were randomly divided into two groups:group A(n=144),treated with heat-sensitive moxibustion(50 sessions)and group B(n=144),treated with Seretide(salmeterol50μg/fluticasone 250μg,twice a day).The scores of asthma control test(ACT),forced expiratory volume in 1 second(FEV1),peak expiratory flow(PEF),and attack frequency were measured after 15,30,60,and 90 days of treatment.Patients followed up3 and 6 months after treatment.RESULTS:There was a significant difference(P=0.0002)in the ACT score and lung function between the two groups after 3 months of treatment and(P=0.000 03)during the follow-up visits.In addition,heat-sensitive moxibustion reduced attack frequency in the period from inclusion to the6-month follow-up visit.CONCLUSION:This study shows that heat-sensitive moxibustion may have a comparable curative effect toSeretide(salmeterol/fluticasone)on asthma. | Rixin Chen Mingren Chen Jun Xiong Zhenhai Chi B0 Zhang Ning Tian Zhenhua Xu Tangfa Zhang Wanyao Li Wei Zhang Xiaofeng Rong Zhen Wang Gang Sun Baohe Ge Guoxiong Yu Nanchang Song | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 20 |
| 19 | High-efficiency breeding of early-maturing rice cultivars via CRISPR/Cas9-mediated genome editing显示文摘Rice is a staple food for more than half of the human population.It has been estimated that by 2030,40%more rice needs to be produced in order to meet the growing demand(Khush,2005).One of the strategies to improve rice productivity is to enlarge rice | Xiufeng Li Wenjia Zhou Yuekun Ren Xiaojie Tian Tianxiao Lv Zhenyu Wang Jun Fang Chengcai Chu Jie Yang Qingyun Bu | 2017 | Journal of Genetics and Genomics2017，44，3： | 20 |
| 20 | Combination therapy reduces the percutaneous coronary intervention acute myocardial infarction incidence of no-reflow after primary in patients with ST-segment elevation显示文摘 | Shan-Shan ZHOU Feng TIAN Yun-Dai CHEN Jing WANG Zhi-Jun SUN Jun GUO Qin-Hua JIN | 2015 | Journal of Geriatric Cardiology2015，12，2： | 18 |
您所在的机构暂未开通此部分的功能，请联系管理员