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| 1 | The Genome of Dendrobium officinale Illuminates the Biology of the Important Traditional Chinese Orchid Herb显示文摘 | Liang Yan Xiao Wang Hui Liu Yang Tian Jinmin Lian Ruijuan Yang ShumeiHao Xuanjun Wang Shengchao Yang Qiye Li Shuai Qi Ling Kui Moses Okpekum Xiao Ma Jiajin Zhang Zhaoli Ding Guojie Zhang Wen Wang Yang Dong Jun Sheng | 2015 | Molecular Plant2015，8，6： | 64 |
| 2 | Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets显示文摘Antibiotic resistance of pathogens,which is caused by the abuse of in-feed antibiotics,threatens the sustainable development of livestock production.The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide(PIAP)as an alternative to in-feed antibiotics in terms of growth performance,intestinal morphology,digestive enzymes and immunity,and microbiota community of the post-weaning piglets.A total of 204 piglets(Duroc×Landrace×Yorkshire,weaned at 28 d age)with a similar body weight of 7.97±1.04 kg were randomly allocated to 4 groups(51 piglets per group):(1)control group:basal diet;(2)AB group:antibiotic,basal diet+chlortetracycline(1000 mg/kg from d 1 to24;500 mg/kg from d 25 to 37);(3)P1 group:basal diet+a relatively low dose of PIAP(400 mg/kg from d 1 to 24;300 mg/kg from d 25 to 37);(4)P2 group,basal diet+a relatively high dose of PIAP(600 mg/kg from d 1 to 24;500 mg/kg from d 25 to 37).The results showed that serum indicators of hepatocyte damage and relative organ weight were not affected by these treatments(P>0.05).Compared with the AB treatment,the P1 treatment remarkably decreased jejunal crypt depth and increased jejunal and ileal villus height:crypt depth ratio(P<0.05).The values of jejunal maltase,lactase,sucrase,intestinal alkaline phosphatase,and secretory immunoglobulin A(SIg A)in the P1 group were sharply increased compared with those in the control and P2 groups(P<0.05).Compared with the control group,the P1 group decreased serum concentrations of D-lactate,diamine oxidase,and endotoxin(P<0.05),and increased the abundance of Lactobacillus reuteri(P<0.05)in the colonic feces.Furthermore,there was a positive correlation between the abundance of L.reuteri and the concentrations of maltase,lactase,sucrase,and SIg A(P<0.05).Collectively,dietary supplementation with a relatively low dose of PIAP(400 mg/kg from d 1 to 24;300 mg/kg from d 25 to 37)demonstrates beneficial effects on intestinal morphology,digestive enzymes,immunity,and permeability by shaping the gut microbiota composition in weaned piglets.This study will provide a valuable reference for using PIAP as an in-feed antibiotic alternative in swine production. | Fengjie Ji Huansheng Yang Qiye Wang Jianzhong Li Hanlin Zhou Shengmin Liu | 2023 | Animal Nutrition2023，，3： | 3 |
| 3 | Changes in progenitors and differentiated epithelial cells of neonatal piglets显示文摘This study aimed to assess the changes of small intestinal morphology,progenitors,differentiated epithelial cells,and potential mechanisms in neonatal piglets.Hematoxylin and eosin staining of samples from 36 piglets suggested that dramatic changes were observed in the jejunum crypts depth and crypt fission index of neonatal piglets(P<0.001).The number of intestinal stem cells(ISC)tended to increase(P<0.10),and a decreased number of enteroendocrine cells appeared in the jejunal crypt on d 7(P<0.05).Furthermore,the mRNA expression of jejunal chromogranin A(ChgA)was down-regulated in d 7 piglets(P<0.05).There was an up-regulation of the adult ISC marker gene of SPARC related modular calcium binding 2(Smoc2),and Wnt/b-catenin target genes on d 7(P<0.05).These results were further verified in vitro enteroid culture experiments.A mass of hollow spheroids was cultured from the fetal intestine of 0-d-old piglets(P<0.001),whereas substantial organoids with budding and branching structures were cultured from the intestine of 7-d-old piglets(P<0.001).The difference was reflected by the organoid budding efficiency,crypt domains per organoid,and the surface area of the organoid.Furthermore,spheroids on d 0 had more Ki67-positive cells and enteroendocrine cells(P<0.05)and showed a decreasing trend in the ISC and goblet cells(P<0.10).Moreover,the mRNA expression of spheroids differed markedly from that of organoids,with low expression of intestinal differentiation gene(Lysozyme;P<0.05),epithelial-specific markers(Villin,E-cadherin;P<0.05),and adult ISC markers(leucine-rich repeat-containing G protein-coupled receptor 5[Lgr5],Smoc2;P<0.001),and upregulation of fetal marker(connexin 43[Cnx43];P<0.05).The mRNA expression of relevant genes was up-regulated,and involved in Wnt/b-catenin,epidermal growth factor(EGF),Notch,and bone morphogenetic protein(BMP)signaling on d 7 organoids(P<0.05).Spheroids displayed low differentiated phenotype and high proliferation,while organoids exhibited strong differentiation potential.These results indicated that the conversion from the fetal progenitors(spheroids)to adult ISC(normal organoids)might largely be responsible for the fast development of intestinal epithelial cells in neonatal piglets. | Lanmei Yin Jun Li Yitong Zhang Qing Yang Cuiyan Yang Zhenfeng Yi Yuebang Yin Qiye Wang Jianzhong Li Nengshui Ding Zhigang Zhang Huansheng Yang Yulong Yin | 2022 | Animal Nutrition2022，，1： | 3 |
| 4 | Dietary high protein-induced diarrhea and intestinal inflammation by activation of NF-kB signaling in piglets显示文摘The present study aimed to investigate whether inflammation-associated responses in piglets are induced by high protein(HP)through activating nuclear factor kappa B(NF-κB)signaling.Sixteen piglets(35 d of age,Duroc×[Landrace×Yorkshire],weaned at d 21,initial BW=9.70±0.11 kg)were allocated to 18%and 26%CP(HP group)at random,comprising 8 replicate pens per treatment.The piglets were slaughtered to collect intestinal tissues when apparent,persistent,and stable diarrhea syndromes happened(on d 12).No significant differences were observed in their growth performance(P>0.05),but reduction by 19.11%,25.31%,23.64%of ADFI,ADG,and G:F,respectively was detected in the HP group.The HP group had greater(P=0.002)diarrhea rates.Furthermore,dietary HP had lower ileal villus height(VH;P=0.048),ratio of villus height to crypt depth(VH/CD ratio;P=0.016),and colonic CD(P=0.034),as well as had the trend(P=0.075)to reduce the ileal villus absorptive area.Moreover,HP diets significantly elevated the goblet cell numbers in the ileal villi(P=0.016)and colonic crypts(P<0.001)and up-regulated(P=0.012)the mRNA expression of mucin2(Muc2)in the ileum.In addition,HP diets increased the myeloperoxidase concentration in the ileum(P=0.002)and colon(P=0.007)of piglets.Dietary HP significantly down-regulated the mRNA expression of tumor necrosis factor-α(TNF-α;P<0.001)in the ileum,induced nitric oxide synthase(iNOS;P=0.040)and interleukin-22(IL-22;P=0.008)in the colon,and inclined to down-regulate interleukin-1β(IL-1β;P=0.076)expression in the colon.The relative protein abundance of Galectin-3(P=0.046)in the colon and the ratio of phosphorylation NF-κB to NF-κB(p-NF=κB/NF-κB ratio)in the ileum of HP piglets were also greater(P=0.038).These results suggest that dietary HP may cause diarrhea in piglets by activating NF-icB signaling induced intestinal inflammation. | Lanmei Yin Jun Li Meiwei Wang Qiye Wang Jianzhong Li Nengshui Ding Huansheng Yang Yulong Yin | 2021 | Animal Nutrition2021，，4： | 3 |
| 5 | Effects ofβ-alanine on intestinal development and immune performance of weaned piglets显示文摘Beta-alanine is an important amino acid involved in several metabolic reactions in the body.The study aimed to investigate the effect ofβ-alanine supplementation on intestinal development and the immune performance of weaned piglets.Thirty-two 21-day-old healthy weaned piglets(half female and half male;Duroc×Landrace×Yorkshire)with an initial body weight of 8.11±0.21 kg were randomly divided into 4 groups with 8 replicates of 1 pig each.The control group was fed a basal diet and the three experimental treatment groups were fed diets supplemented with 300,600 and 1,200 mg/kgβ-alanine,respectively.The trial lasted 28 days and the diets fed were divided into 2 phases:the late lactation period(day 1 to 14)and the nursery period(day 15 to 28),during which the weaned piglets had free access to food and water.The regulatory effects ofβ-alanine were further investigated in vitro using organoids obtained from the jejunum of piglets.In vivo,the addition ofβ-alanine to the diet had no significant effect on the growth performance of weaned piglets(P>0.05),but significantly reduced serum levels of immunoglobulin G(IgG)(P<0.01),immunoglobulin M(IgM)(P=0.005),and complement 3(C3)(P=0.017).The serum interleukin-6(IL-6)levels(P<0.01)were significantly reduced in the 1,200 mg/kg treatment group.The addition ofβ-alanine increased ileal villus height,with the most significant effect at a concentration of 300 mg/kg(P=0.041).The addition of 600 mg/kgβ-alanine significantly up-regulated the expression of superoxide dismutase(SOD)activity(P=0.020)and the zonula occludens-1(ZO-1)gene(P=0.049)in the jejunum.Diets supplemented with 300 mg/kgβ-alanine significantly increased the number of Ki67 positive cells in the jejunal crypts(P<0.01).In vitro,β-alanine increased the organoid budding rates(P=0.001)and the budding height of the crypt significantly(P=0.004).In conclusion,β-alanine can improve intestinal morphology and barrier function,reduce inflammatory responses and alleviate the adverse effects of weaning stress on piglet intestinal health. | Linlin Chen Yan Zhong Xiangqin Ouyang Chunfeng Wang Lanmei Yin Jing Huang Yali Li Qiye Wang Junyan Xie Pengfei Huang Huansheng Yang Yulong Yin | 2023 | Animal Nutrition2023，，1： | 2 |
| 6 | Two kinds of ketoprofen enteric gel beads(CA and CS-SA) using biopolymer alginate显示文摘To obtain expected rapid-release and sustained-release of ketoprofen gel beads, this paper adopted biopolymer alginate to prepare alginate beads and chitosan-alginate gel beads. Formulation factors were investigated and optimized by the single factor test. The release of ketoprofen from calcium alginate gel beads in pH 1.0 hydrochloric acid solution was less than 10% during 2 h, then in pH6.8 was about 95% during 45 min, which met the requirements of rapid-release preparations. However, the drug release of chitosan-alginate gel beads in pH1.0 was less than 5% during 2 h, then in pH6.8 was about 50% during 6 h and reached more than 95% during 12 h, which had a good sustained-release behavior. In addition, the release kinetics of keteprofen from the calcium alginate gel beads fitted well with the Korsmeyer–Peppas model and followed a case-II transport mechanism. However, the release of keteprofen from the chitosan-alginate gel beads exhibited a non-Fickian mechanism and based on the mixed mechanisms of diffusion and polymer relaxation from chitosanalginate beads. In a word, alginate gel beads of ketoprofen were instant analgesic, while chitosan-alginate gel beads could control the release of ketoprofen during gastrointestinal tract and prolong the drug's action time. | Bingchao Cheng Dongyang Li Qiye Huo Qianqian Zhao Qi Lan Mengsuo Cui Weisan Pan Xinggang Yang | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，2： | 2 |
| 7 | In-situ growth of MAX phase coatings on carbonised wood and their terahertz shielding properties显示文摘Electromagnetic interference(EMI)shielding materials have received considerable attention in recent years.The EMI shielding effectiveness(SE)of materials depends on not only their composition but also their microstructures.Among various microstructure prototypes,porous structures provide the advantages of low density and high terahertz wave absorption.In this study,by using carbonised wood(CW)as a template,1-mm-thick MAX@CW composites(Ti2AlC@CW,V2A1C@CW,and Cr2AlC@CW)with a porous structure were fabricated through the molten salt method.The MAX@CW composites led to the formation of a conductive network and multilayer interface,which resulted in improved EMI SE.The average EMI SE values of the three MAX@CW composites were>45 dB in the frequency of 0.6-1.6 THz.Among the composites,V2A1C@CW exhibited the highest average EMI SE of 55 dB. | Jiaxuan HUANG Hujie WAN Mian LI Yiming ZHANG Jianfeng ZHU Xuelin LI Wenchao SHUI Yao LI Xiaomeng FAN Qiye WEN Xu XIAO Qing HUANG | 2021 | Journal of Advanced Ceramics2021，10，6： | 2 |
| 8 | The rheological behavior of EPDM and POE engage produced by CGC and Insitetm technology显示文摘 | Wu Qiye Li Peng Mu Jingxia | 2006 | J Appl Polym Sci2006，101，5： | 1 |
| 9 | Continuity in quantitative domains显示文摘 | Zhang Qiye Fan Li | 2005 | Fuzzy Sets and Systems2005，154，： | 1 |
| 10 | All-dielectric terahertz metasurface for linearly-polarized multichannel transmission and superposition states of spherical and vortex waves显示文摘Compared to pure vortex waves,the superposition state of spherical waves and vortex waves has enough degrees of freedom to upgrade applications in particle manipulation,information encryption,and large-capacity communications.Here,we propose a new scheme to achieve superposition states and multichannel transmission of vortex and spherical waves.Two transmissive all-silicon metasurfaces that enable mutual interference between linearly polarized(LP)waves in the terahertz region are demonstrated.Type A can achieve interference between x and y polarized waves,while type B can achieve interference between x(or y)and x(or y)polarized waves.The multichannel transmission and superposition states of topological charges of+3,+2,and+4 are designed and demonstrated from theoretical,simulative,and experimental perspectives at 1.1 THz.In addition,the objective fact that the focused superposition state must be observed close to the focal plane is also revealed.The measured results are in good agreement with the theoretical and simulative results.This work provides an idea for the design of ultrathin terahertz devices and could be applied in the fields of information encryption and highfrequency communications. | FUYU LI YUANXUN LI TINGTING TANG YONGCHENG LU XINYAN LIU YULONG LIAO QIYE WEN | 2023 | Photonics Research2023，11，3： | 0 |
| 11 | Mechanism of iron on the intestinal epithelium development in suckling piglets显示文摘This study aimed to investigate the mechanism of iron on intestinal epithelium development of suckling piglets. Compared with newborn piglets, 7-day-old and 21-day-old piglets showed changes in the morphology of the jejunum, increased proliferation,differentiated epithelial cells, and expanded enteroids. Intestinal epithelium maturation markers and iron metabolism genes were significantly changed. These results suggest that lactation is a critical stage in intestinal epithelial development, accompanied by changes in iron metabolism. In addition, deferoxamine(DFO) treatment inhibited the activity of intestinal organoids at passage 4(P4) of 0-day-old piglets, but no significant difference was observed in epithelial maturation markers at passage 1(P1) and P4,and only argininosuccinate synthetase 1(Ass1) and β-galactosidase(Gleb) were up-regulated at passage 7(P7). These results in vitro show that iron deficiency may not directly affect intestinal epithelium development through intestinal stem cells(ISCs). The iron supplementation significantly down-regulated the m RNA expression of interleukin-22 receptor subunit alpha-2(IL-22RA2)in the jejunum of piglets. Furthermore, the m RNA expression of IL-22 in 7-day-old piglets was significantly higher than that in0-day-old piglets. Adult epithelial markers were significantly up-regulated in organoids treated with recombinant murine cytokine IL-22. Thus, IL-22 may play a key role in iron-affecting intestinal epithelium development. | Lanmei Yin Yitong Zhang Jun Li Jing Zhou Qiye Wang Jing Huang Jianzhong Li Huansheng Yang | 2023 | Science China(Life Sciences)2023，66，9： | 0 |
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