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| 1 | 微囊藻毒素-LR对人肝癌HT17细胞周期及细胞凋亡的影响显示文摘目的研究微囊藻毒素-LR(MC-LR)对人肝癌HT17细胞周期、凋亡的影响及其可能的作用机制。方法调整HT17细胞密度为1×104/孔,分别设终浓度为0(溶剂对照,0.1%DMSO)、0.05、0.1、0.2、0.4、0.8、2.0、5.0μmol/L的MC-LR染毒组和去铁敏(DFO,1 mmol/L)染毒组以及MC-LR(0.8μmol/L)+DFO(1 mmol/L)染毒组培养24 h,采用四甲基偶氮噻唑蓝(MTT)法检测细胞生长情况。调整HT17细胞密度为1×105/孔,分别设终浓度为0(溶剂对照)、MC-LR(0.8μmol/L)染毒组和DFO(1 mmol/L)染毒组以及DFO(1 mmol/L)+MC-LR(0.8μmol/L)染毒组培养24、48 h,采用流式细胞术检测细胞周期和细胞凋亡情况。结果高剂量(≥0.8μmol/L)MC-LR染毒24 h后细胞存活率明显低于溶剂对照组(P<0.01),DFO+MC-LR染毒组细胞存活率高于0.8μmol/L MC-LR染毒组(P<0.05)。与溶剂对照组相比,MC-LR染毒24、48 h后G0/G1期细胞所占比例和细胞凋亡率均增高,S期细胞所占比例下降(P<0.05或P<0.01);DFO+MC-LR染毒组与MC-LR染毒组染毒24 h后各期细胞所占比例无差异(P>0.05)。DFO+MC-LR染毒组染毒24 h后细胞凋亡率低于MC-LR染毒组(P<0.01)。结论 MC-LR可导致HT17细胞在G0/G1期发生阻滞及细胞凋亡,可能与其诱发的氧化应激、蛋白质磷酸化状态紊乱有关。 | 农清清 Takeuchi T Komatsu M 张志勇 何敏 | 2011 | 环境与健康杂志2011，28，6： | 3 |
| 2 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 3 | Ellectrochemical deposition of SnS thin films显示文摘 | Ichimura M Takeuchi K Ono Y | 2000 | Thin Solid Films2000，98，： | 2 |
| 4 | 胆固醇酯转移蛋白基因的蛋白质截断型变异体与冠状动脉性心脏病风险的关系显示文摘随机对照试验结果表明,抑制胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)的疗法并不能降低冠状动脉性心脏病(coronary heart disease,CHD)的发生风险。研究失败的可能原因包括靶目标无效、靶目标外小分子的不良反应和随机对照设计因素影响等。在编码药物靶点的基因中具有天然存在的遗传变异,以此为基础,人类研究可以深入了解针对基因产物的治疗的潜在功效和安全性。 | Nomura A Won HH Khera AV Takeuchi F Ito K McCarthy S Emdin CA Klarin D Natarajan P Zekavat SM Gupta N Peloso GM Borecki IB Teslovich TM Asselta R Duga S Merlini PA Correa A Kessler T Wilson JG Bown MJ Hall AS Braund PS Carey DJ Murray MF Kirchner HL Leader JB Lavage DR Manus JN Hartze DN Samani NJ Schunkert H Marrugat J Elosua R McPherson R Farrall M Watkins H Juang JJ Hsiung CA Lin SY Wang JS Tada H Kawashiri MA Inazu A Yamagishi M Katsuya T Nakashima E Nakatochi M Yamamoto K Yokota M Momozawa Y Rotter JI Lander ES Rader DJ Danesh J Ardissino D Gabriel S Willer CJ Abecasis GR Saleheen D Kubo M Kato N Ida Chen YD Dewey FE Kathiresan S 刘莉 叶鹏 | 2017 | 中华高血压杂志2017，25，9： | 2 |
| 5 | Protective effect of Irsogladine on monochloramineinduced gastric mucosallesions in rats:a comparative study with rebamipide显示文摘AIM To examine the effect of irsogladine, a novel antiulcer drug, on the mucosal ulcerogenic response to monochloramine (NH 2Cl) in rat stomach, in comparison with rebamipide, another antiulcer drug with cytoprotective activity. METHODS AND RESULTS Oral administration of NH 2Cl (120*!mM) produced severe hemorrhagic lesions in unanesthetized rat stomachs. Both irsogladine ( 1*!mg/*!kg - 10*!mg/*!kg , po ) and rebamipide ( 30*!mg/*!kg - 100*!mg/*!kg , po ) dose dependently prevented the development of these lesions in response to NH 2Cl, the effect of irsogladine was significant at 3*!mg/*!kg or greater, and that of rebamipide only at 100*!mg/*!kg . The protective effect of irsogladine on NH 2Cl induced gastric lesions was significantly reduced by N G nitro L arginine methyl ester (L NAME) but not by indomethacin, while that of rebamipide was significantly mitigated by indomethacin but not by L NAME. Topical application of NH 2Cl (20*!mM) caused a marked reduction of potential difference (PD) in ex vivo stomachs. This PD reduction was not affected by mucosal application of irsogladine, but significantly prevented by rebamipide. The mucosal exposure to NH 4OH (120*!mM) also caused a marked PD reduction in the ischemic stomach (bleeding from the carotid artery), resulting in gastric lesions. These ulcerogenic and PD responses caused by NH 4OH plus ischemia were also significantly mitigated by rebamipide, in an indomethacin sensitive manner, while irsogladine potently prevented such lesions without affecting the PD response, in a L NAME sensitive manner. CONCLUSION These results suggest that ① NH 2Cl generated either exogenously or endogenously damages the gastric mucosa, ② both irsogladine and rebamipide protect the stomach against injury caused by NH 2Cl, and ③ the mechanism underlying the protective action of irsogladine is partly mediated by endogenous nitric oxide, while that of rebamipide is in part mediated by endogenous prostaglandins. | H Yamamoto, M Umeda, H Mizoguchi, S Kato and K Takeuchi | 1999 | World Journal of Gastroenterology1999，5，6： | 2 |
| 6 | An adult case of skeletal open bite with severely narrowed maxillary dental arch显示文摘 | Takeuchi M Tanaka E Nonoyama D | 2002 | Angle Orthod2002，72，4： | 1 |
| 7 | Telmisartan is a promising cardiometabolicsartan due to its unique PPAR-gamma-inducing property显示文摘 | Yamagishi S Takeuchi M | 2005 | Med Hypotheses2005，64，3： | 1 |
| 8 | Potential therapeutic implication of nifedipine,a dihydropyridine-based calcium antagonist,in advanced glycation end product (AGE) -related disorders 显示文摘 | Yamagishi S Nakamura K Takeuchi M | 2005 | Med Hypotheses2005，65，2： | 1 |
| 9 | Regional adjuvant chemotherapy after partial hepatectomy for metastatic colorectal carcinoma 显示文摘 | Takeuchi Y Yasui M | 1997 | Semin Oncol1997，24，26： | 1 |
| 10 | Pigment epithelium-derived factor inhibits oxidative stress-induced apoptosis and dysfunction of cultured retinal pericytes显示文摘 | Amano S Yamagishi S Inagaki Y Nakamura K Takeuchi M Inoue H | 2005 | Microvasc Res2005，69，12： | 1 |
| 11 | Suppression of experimental autoimmune uveoretinitis by inducing differentiation of regulatory T cells via activation of aryl hydrocarbon receptor显示文摘 | Zhang L Ma J Takeuchi M | 2010 | Invest Ophthalmol Vis Sci2010，51，4： | 1 |
| 12 | Karyotype at diagnosis is the major prognostic factor predicting relapse-free survival for patients with Philadelphia chromosome-positive acute lymphoblastic leukemia treated with imatinib-combined chemotherapy显示文摘 | Yanada M Takeuchi J Sugiura I | 2008 | Haematologica2008，93，2： | 1 |
| 13 | Postprandial hypotension due to a lack of sympathetic compensation in patients with diabetes mellitus显示文摘 | Tanakaya M Takahashi N Takeuchi K | 2007 | Acta Med Okayama2007，61，4： | 1 |
| 14 | Genome and virulence deter- minants of high virulence community-acquired MRSA 显示文摘 | Baba T Takeuchi F Kuroda M | 2002 | The Lancet2002，359，5： | 1 |
| 15 | Pyrolytic carbon nanotubes from vapor-grown carbon fibers 显示文摘 | ENDO M TAKEUCHI K KROTO H | 1995 | Carbon1995，33，7： | 1 |
| 16 | Phenolic compounds from Coix lachryrrtal-jobi var Ma-yuen 显示文摘 | Otsuka H Takeuchi M Inoshiri S | 1989 | Phytochemistry1989，28，： | 1 |
| 17 | The Design and Devel- opment of Highly Reactive Titanium Oxide Photocat- alysts Operating under Visible Light Irradiation显示文摘 | ANPO M TAKEUCHI M | 2003 | Catal2003，216，12： | 1 |
| 18 | Synthesis, characterization and photocatalytic reac- tivity of Ti-containing micro-and mesoporous materials 显示文摘 | Hu Y Rakhmawaty D Matsuoka M Takeuchi M Anpo M | 2006 | Journal of Porous Materials2006，13，3： | 1 |
| 19 | Hydrogen production from waste aluminum at different temperatures with LCA显示文摘 | Hiraki T Takeuchi M Hisa M | 2005 | Materials Transactions2005，46，5： | 1 |
| 20 | Simultaneous determination ofgingerols and shogaol using capillary liquid chromatography and itsapplication in discrimination of three ginger varieties from Indonesia显示文摘 | Rafi M Lim LW Takeuchi T | 2013 | Talanta2013，103，： | 1 |
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