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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment. | Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 23 |
| 3 | Occurrence, sources and fate of pharmaceuticals and personal care products in the groundwater: A review显示文摘The presence of pharmaceuticals and personal care products(PPCPs)in the aquatic environment may pose potential threat to the ecosystem and human health,hence PPCPs have aroused much concern over the world.The contamination of PPCPs in the groundwater,the main source of drinking water supply in many countries and regions,has been extensively studied in the last decade.This paper reviews the occurrence of frequently detected PPCPs,including antibiotics,anti-inflammatories,lipid-regulators,carbamazepine,caffeine,and N,N-diethyl-m-toluamide in groundwater,with special concern to the progress made over the past three years.Possible emission sources for PPCPs in groundwater,such as wastewater and contaminated surface water,landfills,septic systems,livestock breeding and sewer leakage,are summarized.Besides,adsorption,migration and degradation,the dominant mechanisms in the subsurface transport and fate of PPCPs,are discussed,and the insights into the future study of PPCPs in the groundwater are provided. | Qian Sui Xuqi Cao Shuguang Lu Wentao Zhao Zhaofu Qiu Gang Yu | 2015 | Emerging Contaminants2015，1，1： | 20 |
| 4 | Rapid determination of pharmaceuticals from multiple therapeutic classes in wastewater by solid-phase extraction and ultra-performance liquid chromatography tandem mass spectrometry显示文摘A new analytical method utilizing ultra-performance liquid chromatography (UPLC) tandem mass spectrometry (MS/MS) has been developed to determine 16 pharmaceuticals from 8 therapeutic classes in wastewater: bezafibrate, clofibric acid, carbamazepine, caffeine, chloramphenicol, diclofenac, gemfibrozil, indomethacin, ketoprofen, mefenamic acid, metoprolol, nalidixic acid, N,N-diethyl-metatoluamide, propranolol, sulpiride and trimethoprim. Key parameters of MS/MS, UPLC and solid phase extraction (SPE) were optimized. In general, recovery of target pharmaceuticals was over 70% for the wastewater effluent samples and 50% for the influent samples. The effects of matrix suppression, loss during the pretreatment as well as instrument variability were successfully corrected by two internal standards, and acceptable relative recovery was obtained. Target compounds were quantitatively analyzed using multiple reaction monitoring (MRM) mode, and the detection limits ranged from 0.3 to 20 ng/L. A detailed study, matrix effect in effluent wastewater was also present. The method was applied to detecting pharmaceuticals in the wastewater from three wastewater treatment plants (WWTPs) in Beijing, China and the results demonstrated that most target compounds were detectable in both the influent and effluent, with the mean concentrations ranging from 20.5 to 5775.6 ng/L and 4.6 to 418.6 ng/L, respectively. | SUI Qian HUANG Jun DENG ShuBo YU Gang | 2009 | Chinese Science Bulletin2009，54，24： | 16 |
| 5 | Induction of biliary cholangiocarcinoma cell apoptosis by ^103pd cholangial radioactive stent γ-rays显示文摘最近的年里的背景, interventional 肿瘤治疗,通过经皮的trans肝的刺包含 intra-cholangial 金属 stents 的培植,为对待 cholangiocarcinoma 提供了一个新方法,放射性的 stents 能集中的 103Pd cholangial 高放射性的剂量进恶意的肿瘤和杀死肿瘤房间有效地,为了阻止腔的重新狭窄,由一个 relapsed 肿瘤引起了。学习是调查 y 光线的功效的礼品的目的由 103Pd 释放了胆汁的管在经由胆汁的 cholangiocarcinoma 房间 apoptosis.Methods 的正式就职对待 cholangiocarcinoma 的放射性的 stent 一组胆汁的管癌症房间一起与 y 光线的剂量被对待。房间然后被3-( 4,5-dimethyl thiazol-2-yl )检验为决定胆汁的管癌症房间的抑制率,以及与另外的方法包括电子显微镜学的 -2,5-diphenyl terazolium溴化物( MTT )技术, DNA agarose 胶化电气泳动,并且流动 cytometry 被申请他们的词法、生物化学的特征的评估。生长曲线和房间的生长抑制率被决定,并且在 cholangiocarcinoma 房间和 DNA 电气泳动乐队的超微结构的变化在y光线由 103Pd 释放了的 UV-lamp.Results 下面被检验禁止的 cholangiocarcinoma 房间生长,是当细胞的生长率被一条光线与比 197.321 MBq 大的剂量震惊时,示威了。cholangiocarcinoma 房间 apoptosis 的典型特征在 197.321 MBq 剂量组被观察,当当在 245.865 MBq 上面由剂量照耀时,房间坏死被观察时。DNA agarose 胶化电气泳动结果在 197.321 MBq 照耀剂量组, 245.865 MBq 照耀剂量组,和控制之间是不同的提供 197.321 MBq 的放射性的剂量的放射性的 stents 是的 group.Conclusions 103Pd 在 cholangiocarcinoma 的治疗有效;103Pd 放射性的 stents 应该为 cholangiocarcinoma 的临床的处理是有用的。 | HE Gui-jin SUN Dan-dan JI Da-wei SUI Dong-ming YU Fa-qiang GAO Qin-yi DAI Xian-wei GAO Hong JIANG Tao DAI Chao-liu | 2008 | Chinese Medical Journal2008，，11： | 12 |
| 6 | Atorvastatin enhances neurite outgrowth in cortical neurons in vitro via up-regulating the Akt/mTOR and Akt/GSK-313 signaling pathways显示文摘 | Ying JIN Hai-juan SUI Yan DONG Qi DING Wen-hui QU Sheng-xue YU Ying-xin JIN | 2012 | Acta Pharmacologica Sinica2012，33，7： | 10 |
| 7 | Origin of Baotoudong syenites in North China Craton:Petrological, mineralogical and geochemical evidence显示文摘Baotoudong 正长岩 pluton 被定位到 Baotou 城市的东方,内部蒙古,沿着北方中国 Craton (NCC ) 的北边缘的三叠纪的碱的 magmatic 带的最西的部分。锆石 U-Pb 年龄, petrological,矿物学并且 pluton 的 geochemical 数据在这份报纸被获得,抑制它的起源和披风来源特征。pluton 由 nepheline-clinopyroxene 正长岩和碱长石正长岩组成,与 214.7 ? 的锆石 U-Pb 年龄 ?????????? 潡潴摵湯 ????? 甠瑬慲潰慴獳捩 ???????????????????? | NIU Xiao Lu YANG Jing Sui LIU Fei ZHANG Hong Yu YANG Ming Chun | 2016 | Science China Earth Sciences2016，59，1： | 10 |
| 8 | The biological role of the CXCL12/CXCR4 axis in esophageal squamous cell carcinoma显示文摘Esophageal cancer is the eighth most common malignant tumor and the sixth leading cause of cancer-related death worldwide.Esophageal squamous cell carcinoma(ESCC)is the main histological type of esophageal cancer,and accounts for 90%of all cancer cases.Despite the progress made in surgery,chemotherapy,and radiotherapy,the mortality rate from esophageal cancer remains high,and the overall 5-year survival rate is less than 20%,even in developed countries.The C-X-C motif chemokine ligand 12(CXCL12)is a member of the CXC chemokine subgroup,which is widely expressed in a variety of tissues and cells.CXCL12 participates in the regulation of many physiological and pathological processes by binding to its specific receptor,C-X-C motif chemokine receptor type 4(CXCR4),where it causes embryonic development,immune response,and angiogenesis.In addition,increasing evidence indicates that the CXCL12/CXCR4 axis plays an important role in the biological processes of tumor cells.Studies have shown that CXCL12 and its receptor,CXCR4,are highly expressed in ESCC.This abnormal expression contributes to tumor proliferation,lymph node and distant metastases,and worsening prognosis.At present,antagonists and imaging agents against CXCL12 or CXCR4 have been developed to interfere with the malignant process and monitor metastasis of tumors.This article summarizes the structure,function,and regulatory mechanism of CXCL12/CXCR4 and its role in the malignancy of ESCC.Current results from preclinical research targeting CXCL12/CXCR4 are also summarized to provide a reference for the clinical diagnosis and treatment of ESCC. | Xianxian Wu Hongdian Zhang Zhilin Sui Yang Wang Zhentao Yu | 2021 | Cancer Biology & Medicine2021，18，2： | 9 |
| 9 | Atorvastatin prevents amyloid-13 peptide oligomer- induced synaptotoxicity and memory dysfunction in rats through a p38 MAPK-dependent pathway显示文摘 | Ling-ling ZHANG Hai-juan SUI Bing LIANG Han-ming WANG Wen-hui QU Sheng-xue YU Ying JIN | 2014 | Acta Pharmacologica Sinica2014，35，6： | 9 |
| 10 | Rapid removal of bisphenol A on highly ordered mesoporous carbon显示文摘Bisphenol A (BPA) is of global concern due to its disruption of endocrine systems and ubiquity in the aquatic environment.It is important,therefore,that efforts are made to remove it from the aqueous phase.A novel adsorbent,mesoporous carbon CMK-3,prepared from hexagonal SBA-15 mesoporous silica was studied for BPA removal from aqueous phase,and compared with conventional powdered activated carbon (PAC).Characterization of CMK-3 by transmission electron microscopy (TEM),X-ray diffraction,and nitrogen adsorption indicated that prepared CMK-3 had an ordered mesoporous structure with a high specific surface area of 920 m 2 /g and a pore-size of about 4.9 nm.The adsorption of BPA on CMK-3 followed a pseudo second-order kinetic model.The kinetic constant was 0.00049 g/(mg·min),much higher than the adsorption of BPA on PAC.The adsorption isotherm fitted slightly better with the Freundlich model than the Langmuir model,and adsorption capacity decreased as temperature increased from 10 to 40°C.No significant influence of pH on adsorption was observed at pH 3 to 9;however,adsorption capacity decreased dramatically from pH 9 to 13. | Qian Sui Jun Huang Yousong Liu Xiaofeng Chang Guangbin Ji Shubo Deng Tao Xie Gang Yu | 2011 | Journal of Environmental Sciences2011，23，2： | 8 |
| 11 | Recognition of Milankovitch cycles in the stratigraphic record: application of the CWT and the FFT to well-log data显示文摘The authors applied a the combination of Continuous Wavelet Transform (CWT) and Fast Fourier Transform (FFT) methods to gamma ray well-log data from the Q3, G1 and D2 wells. This high-resolution stratigraphic study was based on Milankovitch's orbital cycle theory. It was found that the CWT scale factors, ‘a,’ of 12, 24 and 60 match the ratios of the periodicities of precession, obliquity and eccentricity very well. Nine intervals of the Permo-carboniferous strata were recognized to have Milankovitch cycles in them. For example, section A of well Q3 has 29 precession cycles, 15 obliquity cycles and 7 short eccentricity cycles. The wavelengths are 2.7, 4.4 and 7.8 m for precession, obliquity and eccentricity, respectively. Important geological parameters such as the stratigraphic completeness and the accumulation rate were also estimated. These results provide basic information for further cyclostratigraphic correlation studies in the area. They are of great significance for the study of ancient and future climate change. | YU Ji-feng SUI Feng-gui LI Zeng-xue LIU Hua WANG Yu-lin | 2008 | Journal of China University of Mining and Technology2008，18，4： | 8 |
| 12 | Optical properties of NaY(MoO4)2:Eu3+ nanophosphors prepared by molten salt method显示文摘We synthesized NaY(MoO4)2:Eu3+phosphors of different doping concentrations by a molten salt method.This facile way possesses advantages such as simple process,lower calcination temperature(350℃)and small particle size(70 nm).The crystal system is tetragonal phase and crystal lattice is body centered.The photo luminescence measurements including emission spectra,excitation spectra and fluorescence decay curves were carried out,elucidating that NaY(MoO4)2:Eu3+can be effectively excited by near UV and blue light.Moreover,it can be concluded that Eu3+energy transfer type is exchange interaction.Huang-Rhys factor and the critical energy transfer distance(Rc)were calculated to be 0.043 and 0.995 nm,respectively.Auzel’s model was used to obtain the intrinsic radiative transition lifetime of5 D0 level(τ0=0.923 ms).Furthermore,a calculation method was used to calculate refractive index n of nontransparent NaY(MoO4)2:1 mol%Eu3+phosphor,and n was obtained to be 1.86. | Lin Bai Qingyu Meng Mingxue Huo Yu Sui | 2019 | Journal of Rare Earths2019，37，12： | 8 |
| 13 | Maximum entropy based probabilistic load flow calculation for power system integrated with wind power generation显示文摘Distributed generation including wind turbine(WT) and photovoltaic panel increases very fast in recent years around the world, challenging the conventional way of probabilistic load flow(PLF) calculation. Reliable and efficient PLF method is required to take this chage into account.This paper studies the maximum entropy probabilistic density function reconstruction method based on cumulant arithmetic of linearized load flow formulation,and then develops a maximum entropy based PLF(MEPLF) calculation algorithm for power system integrated with wind power generation(WPG). Compared with traditional Gram–Charlier expansion based PLF(GC-PLF)calculation method, the proposed ME-PLF calculation algorithm can obtain more reliable and accurate probabilistic density functions(PDFs) of bus voltages and branch flows in various WT parameter scenarios. It can solve thelimitation of GC-PLF calculation method that mistakenly gains negative values in tail regions of PDFs. Linear dependence between active and reactive power injections of WPG can also be effectively considered by the modified cumulant calculation framework. Accuracy and efficiency of the proposed approach are validated with some test systems. Uncertainties yielded by the wind speed variations, WT locations, power factor fluctuations are considered. | Bingyan SUI Kai HOU Hongjie JIA Yunfei MU Xiaodan YU | 2018 | Journal of Modern Power Systems and Clean Energy2018，6，5： | 8 |
| 14 | Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis显示文摘Curcumenol,an effective ingredient of Wenyujin,has been reported that exerted its antitumor potential in a few cancer types.However,the effect and molecular mechanism of curcumenol in lung cancer are largely unknown.Here,we found that curcumenol induced cell death and suppressed cell proliferation in lung cancer cells.Next,we demonstrated that ferroptosis was the predominant method that contributed to curcumenol-induced cell death of lung cancer in vitro and vivo for the first time.Subsequently,using RNA sequencing,we found that the long non-coding RNA H19(lncRNA H19)was significantly downregulated in lung cancer cells treated with curcumenol,when compared to untreated controls.Overexpression of lncRNA H19 eliminated the anticancer effect of curcumenol,while lncRNA H19 knockdown promoted ferroptosis induced by curcumenol treatment.Mechanistically,we showed that lncRNA H19 functioned as a competing endogenous RNA to bind to miR-19b-3p,thereby enhanced the transcription activity of its endogenous target,ferritin heavy chain 1(FTH1),a marker of ferroptosis.In conclusion,our data show that the natural product curcumenol exerted its antitumor effects on lung cancer by triggering ferroptosis,and the lncRNA H19/miR-19b-3p/FTH1 axis plays an essential role in curcumenol-induced ferroptotic cell death.Therefore,our findings will hopefully provide a valuable drug for treating lung cancer patients. | Ruonan Zhang Ting Pan Yu Xiang Mingming Zhang Han Xie Zimao Liang Bi Chen Cong Xu Jing Wang Xingxing Huang Qianru Zhu Ziming Zhao Quan Gao Chengyong Wen Wencheng Liu Weirui Ma Jiao Feng Xueni Sun Ting Duan Elaine Lai-Han Leung Tian Xie Qibiao Wu Xinbing Sui | 2022 | Bioactive Materials2022，7，7： | 8 |
| 15 | Surface enhanced Raman spectroscopy signals of mixed pesticides and their identification显示文摘A rapid and sensitive method to identify and analyze mixed pesticides of tricyclazole,paraquat and flusilazole by surface-enhanced Raman scattering has been reported.Aqueous samples can be detected by SERS in low concentrations of 0.01 mg/L, 0.1 mg/L and 2.85 mg/L for individual tricyclazole,paraquat and flusilazole respectively.When mixing the three pesticides in the low concentrations,their characteristic peaks can still be identified from the SERS spectrum of the mixture. | Hui Rong Tang Qing Qing Li Yu Lan Ren Jin Pei Geng Peng Cao Tao Sui Xuan Wang Yi Ping Du | 2011 | Chinese Chemical Letters2011，22，12： | 7 |
| 16 | SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target显示文摘Cytokine storm and multi-organ failure are the main causes of SARS-CoV-2-related death.However,the origin of excessive damages caused by SARS-CoV-2 remains largely unknown.Here we show that the SARS-CoV-2 envelope(2-E)protein alone is able to cause acute respiratory distress syndrome(ARDS)-like damages in vitro and in vivo. | Bingqing Xia Xurui Shen Yang He Xiaoyan Pan Feng-Liang Liu Yi Wang Feipu Yang Sui Fang Yan Wu Zilei Duan Xiaoli Zuo Zhuqing Xie Xiangrui Jiang Ling Xu Hao Chi Shuangqu Li Qian Meng Hu Zhou Yubo Zhou Xi Cheng Xiaoming Xin Lin Jin Hai-Lin Zhang Dan-Dan Yu Ming-Hua Li Xiao-Li Feng Jiekai Chen Hualiang Jiang Gengfu Xiao Yong-Tang Zheng Lei-Ke Zhang Jingshan Shen Jia Li Zhaobing Gao | 2021 | Cell Research2021，31，8： | 7 |
| 17 | Metal halide perovskite nanocrystals and their applications in optoelectronic devices显示文摘In recent years, metal halide perovskite nanocrystals (NCs) have been favored by manyresearchers due to their unique properties including long carrier diffusion length, highcarrier mobility, tunable emission wavelength, and narrow full width at half maximum,making them great application potentials in optoelectronic devices. The photoluminescencequantum yields of perovskite NCs are nearly 100%, and the device efficiencyof perovskite NC-based light-emitting diodes (LEDs) has been improvedsignificantly from below 0.1% to over 20%. In addition, perovskite NC-based solarcells and photodetectors have also developed rapidly in recent years. Here, we summarizethe synthesis and the basic optoelectronic properties of metal halide perovskiteNCs and introduce their applications in LEDs, solar cells, and photodetectors. | Po Lu Min Lu Hua Wang Ning Sui Zhifeng Shi William W.Yu Yu Zhang | 2019 | InfoMat2019，1，4： | 7 |
| 18 | Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes显示文摘Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization.Characterization of the liposomes was conducted,and drug content,entrapment efficiency(EE),in vitro release and stability were studied by ultra-fast liquid chromatography(UFLC)and a liquid surface method.Blood was drawn from rats to establish the pharmacokinetic parameters.The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model.Histological analyses were performed to evaluate safety.Results:The PEG-Lipo-β-E showed good stability and was characterized as 83.31±0.181 nm in size,0.279±0.004 in polydispersity index(PDI),-21.4±1.06 mV in zeta potential,6.65±0.02 in pH,5.024±0.107 mg/mL inβ-elemene(β-E)content,and 95.53±1.712%in average EE.The Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)indicated the formation of PEG-Lipo-β-E.Compared to elemene injection,PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance,a 1.62-fold increase in half-life,and a 1.76-fold increase in area under the concentration-time curves(AUCs)from 0 hour to 1.5 hours(P<0.05).PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo.Histological analyses showed that there was no evidence of toxicity to the heart,kidney,liver,lung or spleen.Conclusions:The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation,high EE,good stability,improved bioavailability and antitumor effects. | Bingtao Zhai Qibiao Wu Wengang Wang Mingming Zhang Xuemeng Han Qiujie Li Peng Chen Xiaying Chen Xingxing Huang Guohua Li Qin Zhang Ruonan Zhang Yu Xiang Shuiping Liu Ting Duan Jianshu Lou Tian Xie Xinbing Sui | 2020 | Cancer Biology & Medicine2020，17，1： | 6 |
| 19 | Knockdown of S-phase Kinase-associated Protein-2 Expression in MCF-7 Inhibits Cell Growth and Enhances the Cytotoxic Effects of Epirubicin显示文摘S 阶段联系 kinase 的 protein-2 (Skp2 ) 在调停 ubiquitin 的解朊作用起一个关键作用,导致房间从的前进一对一个 proliferative 状态静止。Skp2 完了在许多肿瘤表示了并且相反地与 p27 表示相关,包括乳癌。在这研究,我们使用了小介入的 RNA (siRNAs ) 在人的乳癌 MCF-7 细胞禁止 Skp2 表示并且如果击倒,从而由 RNA 干扰(RNAi ) Skp2 调查将禁止乳癌细胞生长并且在 MCF-7 细胞上影响 epirubicin 的效果。三 Skp2 siRNA 构造被重新结合并且短暂地 transfected 人乳癌 MCF-7 房间。Skp2 的下面规定被反向的抄写聚合酶链反应和蛋白质印迹分析证实。我们证明 Skp2 siRNA 转染减少了 Skp2 蛋白质并且在 MCF-7 房间导致了 p27 蛋白质的累积。Skp2 siRNA 在 vitro 并且在 vivo 禁止了房间增长。由 RNAi 的 Skp2 击倒在 vitro 的增加的细胞的 apoptosis。有治疗进一步与 epirubicin 跟随的 Skp2 siRNA 的治疗禁止了癌症房间线的增长,与 epirubicin 跟随的控制 siRNA 相比。因此, Skp2 击倒由 RNAi 的表示禁止乳癌细胞生长并且提高 epirubicin 的效果。Skp2 的调停 siRNA 的基因 silencing 能是为在规定下面的 p27 的抑制的新奇癌症基因治疗。 | Lichun SUN Li CAI Yan YU Qingwei MENG Xuesong CHENG Yanbin ZHAO Guangjie SUI Fengmin ZHANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，12： | 6 |
| 20 | Experimental study on anaphylaxis of Qingkailing injection and its components on Beagle dogs显示文摘OBJECTIVE:To study the anaphylaxis of Qingkailing injection(QI) and its components.METHODS:Experimental anaphylactoid and allergic reactions were used.Changes in the behaviors of Beagles and serum levels of histamine,immunoglobulin(Ig)E,IgG,IgM,eosinophil cationic protein(ECP),and interleukin(IL)-4,as well as blood pressure,after injecting QI and its components on the forelimb veins of Beagles were observed.RESULTS:According to comprehensive determination of abnormal behavior scores and changes in serum levels of histamine,IgE,IgG,IgM,ECP,and IL-4,as well as in blood pressure,radix isatidis and hyodeoxycholic acid caused anaphylactoid reactions,and honeysuckle,radix isatidis,hydrolysate,cholic acid and Gardenia jasminoides caused allergic reactions.The anaphylaxis of QI involved anaphylactoid and allergic reactions.CONCLUSION:QI and its components need to be refined further to improve the safety,efficacy,and quality of its use in clinical settings. | Zhiguo Wang Danqiao Wang Yu Sui Haifeng Cui Youhua Yu | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 6 |
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