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| 1 | Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1显示文摘Background: Resveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects of dietary resveratrol supplementation during pregnancy and lactation on the antioxidant status of sows and piglets and on antioxidant gene expression and pathway in placenta.Methods: Forty sows were allotted to 2 dietary treatments 20 d after breeding. Sows were fed a control diet and a control diet with 300 mg/kg resveratrol. Oxidative stress biomarkers and antioxidant enzymes were measured in the placenta, milk, and plasma of sows and piglets. Antioxidant gene expression and protein expression of Kelch-like ECH-associated protein 1-Nuclear factor E2-related factor 2(Keap1-Nrf2), nuclear factor kappa B-p65(NFκB-p65) and sirtuin1(Sirt1) were quantified in the placenta.Results: Dietary resveratrol increased the litter and piglets weaning weights. Antioxidant status in the milk, placenta and plasma of sows and piglets was partially improved by dietary resveratrol. In placenta, Nrf2 protein expression was increased and Keap1 protein expression was decreased by dietary resveratrol. The m RNA expression of antioxidant genes including catalase(CAT), glutathione peroxidase 1(GPX1), GPX4, superoxide dismutase 1(SOD1)and heme oxygenase 1(HO1), and phase 2 detoxification genes, including glutamate-cysteine ligase modifier(GCLM), microsomal glutathione S-transferase 1(MGST1) and UDP glucuronosyltransferase family 1 member A1(UGT1 A1), was increased by dietary resveratrol. Dietary resveratrol also increased Sirt1 and phosphorylated NFκB-p65 protein expression in the placenta. We failed to observe any influences of dietary resveratrol on pro-inflammatory cytokine levels, including those of interleukin 1β(IL-1β), IL-6, IL-8 and tumor necrosis factor α(TNF-α). However, we observed that the m RNA expression of IL-8 in placenta was reduced by maternal resveratrol. In addition, dietary resveratrol showed interactive effects with day of lactation on activities of SOD and CAT and levels of malonaldehyde(MDA) and hydrogen peroxide(H2 O2) in milk.Conclusions: Dietary resveratrol supplementation during pregnancy and lactation improves the antioxidant status of sows and piglets, which is beneficial to the reproductive performance of sows. Dietary resveratrol regulates placental antioxidant gene expression by the Keap1-Nrf2 pathway and Sirt1 in placenta. | Qingwei Meng Tao Guo Gaoqiang Li Shishuai Sun Shiqi He Baojing Cheng Baoming Shi Anshan Shan | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 33 |
| 2 | A Novel Single-Nucleotide Mutation in a CLAVATA3 Gene Homolog Controls a Multilocular Silique Trait in Brassica rapa L.显示文摘 | Chuchuan Fan, Yudi Wu, Qingyong Yang, Yang Yang, Qingwei Meng, Keqiao Zhang, Jinguo Li, Jinfang Wang Yongming Zhou | 2014 | Molecular Plant2014，7，12： | 12 |
| 3 | A novel tomato SUMO E_3 ligase,SlSIZ_1,confers drought tolerance in transgenic tobacco显示文摘SUMOylation is an important post-translational modification process that regulates different cellular functions in eukaryotes.SIZ/PIAS-type SAP and Miz1(SIZ_1) proteins exhibit SUMO E_3 ligase activity,which modulates SUMOylation.However,SIZ_1 in tomato has been rarely investigated.In this study,a tomato SIZ_1 gene(Sl SIZ_1)was isolated and its molecular characteristics and role in tolerance to drought stress are described.Sl SIZ_1 was upregulated by cold,sodium chloride(Na Cl),polyethylene glycol(PEG),hydrogen peroxide(H_2O_2) and abscisic acid(ABA),and the corresponding proteins were localized in the nucleus.The expression of Sl SIZ_1 in Arabidopsis thaliana(Arabidopsis) siz1-2 mutants partially complemented the phenotypes of dwarf,cold sensitivity and ABA hypersensitivity.Sl SIZ_1 also exhibited the activity of SUMO E_3 ligase to promote the accumulation of SUMO conjugates.Under drought stress,the ectopic expression of Sl SIZ_1 in transgenic tobacco lines enhanced seed germination and reduced the accumulation of reactive oxygen species.SlSIZ_1 overexpression conferred the plants with improved growth,high free proline content,minimal malondialdehyde accumulation and increased accumulation of SUMO conjugates.Sl SIZ_1 is a functional homolog of Arabidopsis SIZ_1 with SUMO E_3 ligase activity.Therefore,overexpression of Sl SIZ_1 enhanced the tolerance of transgenic tobacco to drought stress. | Song Zhang Kunyang Zhuang Shiju Wang Jinlian Lv Na'na Ma Qingwei Meng | 2017 | Journal of Integrative Plant Biology2017，59，2： | 7 |
| 4 | Knockdown of S-phase Kinase-associated Protein-2 Expression in MCF-7 Inhibits Cell Growth and Enhances the Cytotoxic Effects of Epirubicin显示文摘S 阶段联系 kinase 的 protein-2 (Skp2 ) 在调停 ubiquitin 的解朊作用起一个关键作用,导致房间从的前进一对一个 proliferative 状态静止。Skp2 完了在许多肿瘤表示了并且相反地与 p27 表示相关,包括乳癌。在这研究,我们使用了小介入的 RNA (siRNAs ) 在人的乳癌 MCF-7 细胞禁止 Skp2 表示并且如果击倒,从而由 RNA 干扰(RNAi ) Skp2 调查将禁止乳癌细胞生长并且在 MCF-7 细胞上影响 epirubicin 的效果。三 Skp2 siRNA 构造被重新结合并且短暂地 transfected 人乳癌 MCF-7 房间。Skp2 的下面规定被反向的抄写聚合酶链反应和蛋白质印迹分析证实。我们证明 Skp2 siRNA 转染减少了 Skp2 蛋白质并且在 MCF-7 房间导致了 p27 蛋白质的累积。Skp2 siRNA 在 vitro 并且在 vivo 禁止了房间增长。由 RNAi 的 Skp2 击倒在 vitro 的增加的细胞的 apoptosis。有治疗进一步与 epirubicin 跟随的 Skp2 siRNA 的治疗禁止了癌症房间线的增长,与 epirubicin 跟随的控制 siRNA 相比。因此, Skp2 击倒由 RNAi 的表示禁止乳癌细胞生长并且提高 epirubicin 的效果。Skp2 的调停 siRNA 的基因 silencing 能是为在规定下面的 p27 的抑制的新奇癌症基因治疗。 | Lichun SUN Li CAI Yan YU Qingwei MENG Xuesong CHENG Yanbin ZHAO Guangjie SUI Fengmin ZHANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，12： | 6 |
| 5 | Late Cenozoic Stratigraphy and Paleomagnetic Chronology of the Zanda Basin,Tibet, and Records of the Uplift of the Qinghai-Tibet Plateau显示文摘摘要:特征近来新生代构造 Qinghai 西藏高原的南部的边缘高举可以在 Zanda 盆从 fluvio 湖的阶层被推断, Ngari,西藏。在盆的厚fluvio湖的阶层是老的 5.890.78 妈并且他们的免职为 5.11 妈坚持了的 Magnetostratigraphic 学习表演,即在结束开始在早更新世的结束中新世、结束,在比 1.58 妈没有迟了的区域以第四级的冰川的阶段开始。沉积环境的分析显示高原开始了的南部的 Qinghai 西藏上的 Zanda 盆在 5.89 妈,高举比北 Qinghai 西藏高原迟了。在 Guge 和 Qangz | MENG Xiangang ZHU Dagang SHAO Zhaogang YANG Chaobin HAN Jianen YU Jia MENG Qingwei LU Rongping | 2008 | Acta Geologica Sinica(English Edition)2008，82，1： | 6 |
| 6 | LeCDJ1,a chloroplast DnaJ protein,facilitates heat tolerance in transgenic tomatoes显示文摘The roles of a tomato(Lycopersicon esculentum) chloroplast-targeted DnaJ protein(LeCDJ1) were investigated using wild-type(WT) and sense transgenic tomatoes. The LeCDJ1 expression was upregulated by 38 °C, 42 °C, 45 °C, NaCl, PEG, methyl viologen(MV) and hydrogen peroxide(H2 O2), but not by 30 °C and 35 °C. Meanwhile, LeCDJ1 was involved in the response of plants to abscisic acid(ABA). Under heat stress, the sense plants showed better growth, higher chlorophyll content, lower malondialdehyde(MDA) accumulation and relative electrical conductivity(REC), and also less PSII photoinhibition than WT. Interestingly, the sense plants treated with streptomycin(SM), an inhibitor of organellar translation, still showed higher maximum photochemistry efficiency of PSII(Fv/Fm) and D1 protein levels than the SM-untreated WT, suggesting that the protective effect of LeCDJ1 on PSII was, at least partially, independent of D1protein synthesis. Furthermore, the relatively lower superoxide radical(O2·-) and H2 O2 levels in the sense plants were considered to be due to the higher ascorbate peroxidase(APX) and superoxide dismutase(SOD) activity, which seemed unlikely dependent on their transcription level. These results indicated that LeCDJ1 overexpression facilitated heat tolerance in transgenic tomatoes. | Fanying Kong Yongsheng Deng Guodong Wang Jieru Wang Xiaoqing Liang Qingwei Meng | 2014 | Journal of Integrative Plant Biology2014，56，1： | 5 |
| 7 | Transcriptome analysis of the effect of pyrroloquinoline quinone disodium(PQQ·Na2) on reproductive performance in sows during gestation and lactation显示文摘Background: Pyrroloquinoline quinone(PQQ), which is a water soluble, thermo-stable triglyceride-quinone, is widely distributed in nature and characterized as a mammalian vitamin-like redox cofactor. The objective of this study was to investigate the effects of pyrroloquinoline quinone disodium(PQQ·Na2) on reproductive performance in sows.Results: Dietary supplementation with PQQ·Na2 significantly increased the total number of piglets born, the number of piglets born alive and the born alive litter weight. It also increased the antioxidant status in the placenta, plasma and milk. The concentration of NO was significantly increased in the plasma and placenta. RNA-seq analysis showed that462 unigenes were differentially expressed between the control(Con) treatment and PQQ treatment groups.Among these unigenes, 199 were upregulated, while 263 unigenes were downregulated. The assigned functions of the unigenes covered a broad range of GO categories. Reproduction(27, 7.03%) and the reproduction process(27, 7.03%) were assigned to the biological process category. By matching DEGs to the KEGG database, we identified 29 pathways.Conclusions: In conclusion, dietary supplementation with PQQ·Na2 in gestating and lactating sows had positive effects on their reproductive performance. | Boru Zhang Chenxi Wang Wei Yang Hongyun Zhang Qingwei Meng Baoming Shi Anshan Shan | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 5 |
| 8 | Broadband terahertz wave generation from an epsilon-near-zero material显示文摘Broadband light sources emitting in the terahertz spectral range are highly desired for applications such as noninvasive imaging and spectroscopy.Conventionally,THz pulses are generated by optical rectification in bulk nonlinear crystals with millimetre thickness,with the bandwidth limited by the phase-matching condition.Here we demonstrate broadband THz emission via surface optical rectification from a simple,commercially available 19nmthick indium tin oxide(ITO)thin film.We show an enhancement of the generated THz signal when the pump laser is tuned around the epsilon-near-zero(ENZ)region of ITO due to the pump laser field enhancement associated with the ENZ effect.The bandwidth of the THz signal generated from the ITO film can be over 3 THz,unrestricted by the phasematching condition.This work offers a new possibility for broadband THz generation in a subwavelength thin film made of an ENZ material,with emerging physics not found in existing nonlinear crystals. | Wenhe Jia Meng Liu Yongchang Lu Xi Feng Qingwei Wang Xueqian Zhang Yibo Ni Futai Hu Mali Gong Xinlong Xu Yuanyuan Huang Weili Zhang Yuanmu Yang Jiaguang Han | 2021 | Light(Science & Applications)2021，10，1： | 4 |
| 9 | Upwelling Process of the Western Himalaya Mountains:Height and Velocity Estimation Evidenced by Formation and Evolution of the Zanda Basin,Tibet,China显示文摘把地质的调查, stratigraphic 节测量和室内的全面调查基于地, Zanda 盆在 Himalaya 板中的构造地点被查明,并且 Zanda 盆的形成和进化作为六个阶段在到早更新世的上新世期间被分类:(a) 主要指责裂缝的舞台,(b) 快指责裂缝的舞台,(c) 集中的指责裂缝的舞台,(d) 壅滞舞台,(e) 第二等的指责裂缝的舞台,和(f) 第二等的快指责裂缝的舞台。基于 Zanda 盆的这个六阶段的形成进化理论,当下列五上演,到早更新世的从上新世的西方的 Himalaya 山的 upwelling 过程被分类:(a) 慢 upwelling 舞台(5.44.4 妈) ,(b) 中间速度的 upwelling 舞台(4.43.5 妈) ,(c) 快 upwelling 舞台(3.53.2 妈) ,(d)停止 upwelling 阶段(3.22.7 妈) ,和(e) 快 upwelling 舞台(2.7 妈) 。研究从早上新世(4.7 妈) 在持续时间显示出那到上新世(2.67 妈) 的目的,它持续了 203 万年, Himalaya 山高举了在 0.74 mm/a 的速度的 1500 m;这属于中间速度的 upwelling。在在早更新世的早阶段的 131 万年期间, Himalaya 山在 1.15 mm/a 的速度在另一 1500 m 上面升起了;这是相当快的 upwelling。所有这些数据证明了西方的 Himalaya 山的 upwelling 沿着有 multi-stages,多速度,和非天律不变论的特征的一个复杂过程。 | ZHU Dagang MENG Xian' gang SHAO Zhaogang YANG Chaobin HAN Jian'en YU Jia MENG Qingwei Lü Rongping | 2010 | Acta Geologica Sinica(English Edition)2010，84，2： | 3 |
| 10 | Modified halloysite nanotubes reduce the toxic effects of zearalenone in gestating sows on growth and muscle development of their offsprings显示文摘Background: Zearalenone(ZEN) is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees. The present experiment was designed to study the maternal persistent effects of zearalenone toxicity in gestating sows on growth and muscle development of their offsprings, and the alleviation of zearalenone toxicity by modified halloysite nanotubes(MHNTs).Methods: Eighteen sows were fed with one of three dietary treatments that included the following:(1) a control diet,(2) a contaminated grain diet(with 50 % moldy corn, 2.77 mg/kg ZEN), and(3) a contaminated grain diet(with 50 %moldy corn, 2.76 mg/kg ZEN) + 1 % MHNTs. Each sow was exclusively fed its experimental diets from 35 to 70 d of gestation at a total of 2 kg daily. Muscle samples were collected from six piglets per treatment at birth, weaning and finishing.Results: The results showed that feeding the sows with the ZEN-contaminated diets from 35 to 70 d of gestation decreased the ADG, ADFI and G:F of their offsprings(P < 0.05). The muscle fiber numbers in the newborn, weaning and growing-finishing pigs and the muscle fiber diameters at birth and weaning were also decreased by maternal ZEN exposure(P < 0.05). The expressions of IGF-I, IGF-II, Myf-5 and Mstn at birth and IGF-II, Pax7, Myf-5 and Myo D1 at weaning were altered by feeding gestating sows with ZEN-contaminated diets(P < 0.05). The MHNTs reduced most of the ZEN-induced toxic effects: the ADG and ADFI on growth performance, the muscle fiber numbers at weaning and finishing and the muscle fiber diameters at weaning(P < 0.05). The expression levels of IGF-II and Mstn in newborn piglets and IGF-II and Myf-5 in weaning piglets were also prevented by adding 1 % MHNTs(P < 0.05).Conclusions: The present study demonstrated that the offsprings of sows fed with ZEN-contaminated diets from 35 to 70 day of gestation exhibited weakening on growth performance, physiological changes in their muscle fibers and alterations of m RNA expression in their muscle tissues, and also indicated that MHNTs prevented most of the ZENinduced weakening in the muscle tissues. | Rui Gao Qingwei Meng Jianan Li Min Liu Yuanyuan Zhang Chongpeng Bi Anshan Shan | 2016 | Journal of Animal Science and Biotechnology2016，7，4： | 3 |
| 11 | A novel Lyn-like protein tyrosine kinase identified in lamprey and its role in immune response显示文摘The avian sarcoma virus transforming gene(Src)kinase family,a type of nonmreceptor protein tyrosine kinases(nrTPKs),has been reported to play roles in cell growth,division,migration,adhesion,the immune response,and signal transduction berween cells[1,2].The Src family kinases can be divided into three subfamilies based on their conserved domains:SrcA subfamily(Sre,Yes,Fyn,and Fgr,SrcB subfamily(Lck,Hck,Blk,and Lyn),and Frk subfamily[3]. | Tingting Zhao Kedi Luo Xin Liu Shanshan Dong Yinglun Han Meng Gou Peng Su Qingwei Li Ting Zhu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 2 |
| 12 | A quasi-3D Fano resonance cavity on optical fiber end-facet for high signal-to-noise ratio dip-and-read surface plasmon sensing显示文摘Surface plasmon devices mounted at the end-facets of optical fibers are appealing candidates for rapid and point-of-care sensing applications,by offering a special dip-and-read operation mode.At present,these devices’noise-equivalent limits-of-detection lag far behind the free-space counterparts,leaving them incapable of most biosensing applications.Here we report a quasi-3D Fano resonance cavity and its fabrication method to fundamentally improve the quality factor and coupling efficiency for fiber-coupled surface plasmon resonance.In this device,the Fano resonance combines the high coupling efficiency of a Fabry-Pérot etalon and the high quality factor resonance of a plasmonic crystal cavity.The quasi-3D device was fabricated on a planar substrate and transferred to a single-mode fiber end-facet,which requires a low-adhesion yet surface-plasmon-tunneling interface between the device and the planar substrate.Such an interface was realized with a nanocap-slit unit structure,of which the plasmonic crystal was consisted.A noise-equivalent limit of detection of~10-7 RIU was experimentally obtained,allowing bovine serum albumin physical adsorption to be distinguished at ng mL-1 level concentrations.Therefore,breaking through the long-standing signal-to-noise ratio bottleneck,this work makes fiber end-facet surface plasmon devices into one of high sensitivity label-free sensing technologies.At the same time,it provides an enabling top-down fabrication technology for making 3D plasmonic structures on fiber end-facets at the nanometer scale. | Xiaqing Sun Zeyu Lei Hao Zhong Chenjia He Sihang Liu Qingfeng Meng Qingwei Liu Shengfu Chen Xiangyang Kong Tian Yang | 2022 | Light(Advanced Manufacturing)2022，3，4： | 2 |
| 13 | Glycine betaine‐mediated potentiation of HSP gene expression involves calcium signaling pathways in tobacco exposed to NaCl stress显示文摘 | Meifang Li Shangjing Guo Ying Xu Qingwei Meng Gang Li Xinghong Yang | 2014 | Physiol Plantarum2014，，1： | 1 |
| 14 | The Impact of Atmospheric Deposition of Cadmium on Dominant Algal Species in the East China Sea显示文摘Cadmium(Cd) mainly derived from anthropogenic emissions can be transported through atmospheric pathway to marine ecosystem,affecting the phytoplankton community and primary productivity.In this study,we identified the toxicity threshold of Cd for phytoplankton under seawater conditions of the coastal East China Sea(ECS) through both laboratory and in situ mesocosm incubation experiments.The mesocosm experiment showed that Cd in low concentration(0.003 μg per μg chl a) was conducive to the growth of natural community and increased chl a productivity.In high concentration(0.03 μg per μg chl a) Cd acted as an inhibiting factor which decreased the total chl a productivity.The diatom community was found to be more sensitive to Cd toxicity than dinoflagellate,as the low concentration Cd showed toxicity to diatom but enhanced dinoflagellate growth.We noticed that the soluble Cd estimated from atmosphere deposition to the coastal ECS was below the toxicity threshold and the Cd deposition might promote phytoplankton growth in this region.In our laboratory experiments,adding Cd,similar to aerosol deposition,stimulated the growth of both dominant algal species Prorocentrum donghaiense Lu(dinoflagellate) and Skeletonema costatum(diatom).Adding Cd on a higher level inhibited the growth of both the species,but Skeletonema costatum seemed obviously more sensitive to toxicity.This indicates the potential impact of atmospheric deposition Cd on phytoplankton community succession in the ECS. | QUAN Qiwei CHEN Ying MA Qingwei WANG Fujiang MENG Xi WANG Bo | 2016 | Journal of Ocean University of China2016，15，2： | 1 |
| 15 | Biological function of resveratrol and its application in animal production:a review显示文摘With the prohibition of antibiotics in feed,plant functional substances have been widely studied as feed additives.Resveratrol,a natural stilbene,and a non-flavonoid polyphenol found in plants,possesses antioxidant,anti-inflammatory,and metabolic regulatory features.Resveratrol generated intense scientific and public interest,primarily due to its widely reported ability to prevent cancer,delay aging and alleviate related metabolic diseases.Recently,resveratrol has been studied and applied as a feed additive in animal production.This review focuses on the outline of the absorption and metabolism and biological functions of resveratrol and summarizes the application of dietary resveratrol in animal production up to the present,including pigs,poultry,and ruminants.In pigs,dietary resveratrol improved intestinal health,mitochondrial function,meat quality,and more.In poultry,studies have shown that dietary resveratrol improves growth performance and meat and egg quality and alleviates heat stress induced adverse effects.There are few studies on dietary resveratrol in ruminants;however previous studies have indicated that dietary resveratrol increases nutrient digestibility and reduces methane emissions in sheep.It is hoped that this review could provide a specific theoretical basis and research ideas for the research and application of resveratrol. | Qingwei Meng Jiawei Li Chunsheng Wang Anshan Shan | 2023 | Journal of Animal Science and Biotechnology2023，14，3： | 1 |
| 16 | Effects of cold-Hardening on chilling-induced photoinh/bition of photosynthesis and on xanthophyll cycle pigments in sweetpepper显示文摘 | Liu Peng Meng Qingwei Zou Qi | 2001 | Photosynthetica2001，39，3： | 1 |
| 17 | Pharmacokinetics, tissue distribution and excretion study of resveratrol and its prodrug 3,5,4′-tri- O -acetylresveratrol in rats显示文摘 | Li Liang Xueying Liu Qingwei Wang Sikun Cheng Shengyong Zhang Meng Zhang | 2013 | Phytomedicine2013，，6： | 1 |
| 18 | Study on Dielectric Resonator Antenna with Annular Patch for High Gain and Large Bandwidth显示文摘A new high-gain cylindrical Dielectric resonator antenna(DRA) with a large bandwidth is proposed.A cylindrical Dielectric resonator(DR), a double-annular patch and a metallic cylinder are used to obtain a large bandwidth and a high gain. The mode TM12 excited in the patch is used to enhance the gain of the DRA, and the cavity formed by the metallic cylinder provides a further higher gain and a larger bandwidth. The measured results demonstrate that the proposed DRA achieves a large bandwidth of 23% from 5.3 to 6.8GHz with VSWR less than two and a high gain around 11 dBi. | FENG Kuisheng LI Na MENG Qingwei WANG Yongfeng ZHANG Jingwei | 2015 | Chinese Journal of Electronics2015，24，4： | 1 |
| 19 | Elevated Plasma IL-37, IL-18, and IL-18BP Concentrations in Patients with Acute Coronary Syndrome显示文摘 | Qingwei Ji Qiutang Zeng Ying Huang Ying Shi Yingzhong Lin Zhengde Lu Kai Meng Bangwei Wu Kunwu Yu Meng Chai Yuyang Liu Yujie Zhou Alex Kleinjan | 2014 | Mediators of Inflammation2014，，： | 1 |
| 20 | Resveratrol alleviates oxidative stress induced by oxidized soybean oil and improves gut function via changing gut microbiota in weaned piglets显示文摘Background Oxidized soybean oil(OSO)has been shown to impair growth and exacerbate inflammation,leading to intestinal barrier injury in animals.Recent evidence suggests important roles for resveratrol(RES)in the promoting growth performance,antioxidant capacity,anti-inflammatory,and regulate intestinal barriers in animals.Therefore,The objectives of this study are to investigate the effects of dietary RES(purity 98%)supplementation on the growth performance,antioxidant capacity,inflammatory state,and intestinal function of weaned piglets challenged with OSO.Methods A total of 28 castrated weaned male piglets with a similar body weight of 10.197 replications per treatment and±0.10 kg were randomly assigned to 4 dietary treatments for 28-d feeding trial with 1 piglet per replicate.Treatments were arranged as a 2×2 factorial with oil type[3%fresh soybean oil(FSO)vs.3%OSO]and dietary RES(0vs.300 mg/kg).Results The results showed that relative to the FSO group,OSO stress tended to decrease the average daily feed intake(ADFI),and decreased the activity levels of lipase,villus/crypt ratio(VCR),the mRNA expression of FABP1,SOD2,IL-10 and ZO-1 in the jejunum,and SOD2,GPX1,occludin and ZO-1 in the colon,the levels of acetic acid in the colonic digesta,whereas up-regulated the mRNA expression of IL-1βand TNF-αin the jejunum(P<0.05).Moreover,dietary supplementation with RES increased ether extract(EE),the activity levels of sucrase,lipase,α-amylase,villus height(VH)and VCR,the mRNA expression of FABP1,SOD2,IL-10 and occludin in the jejunum,and FABP1,PPAR-γ,GPX1,occludin and ZO-1 in the colon,and the abundance of Firmicutes,acetic and propionic acid,but decreased the levels of D-lactic acid in the plasma,the abundance of Bacteroidetes in the colonic digesta of weaned piglets compared to the non-RES group(P<0.05).Meanwhile,in the interaction effect analysis,relative to the OSO group,dietary RES supplementation in the diets supplemented with OSO increased the activity levels of trypsin,VH in the jejunum,the abundance of Actinobacteria,the levels of butyric acid of weaned piglets,but failed to influence the activity levels of trypsin and VH,Actinobacteria abundance,the levels of butyric acid when diets were supplemented with FSO(interaction,P<0.05).Relative to the OSO group,dietary RES supplementation in the diets supplemented with OSO decreased the activity levels of DAO in the plasma of weaned piglets but failed to influence the activity levels of DAO when diets were supplemented with FSO(interaction,P<0.05).Relative to the FSO group,dietary RES supplementation in the diets supplemented with FSO decreased the level of propionic acid,whereas RES supplementation failed to influence the level of propionic acid when the diet was supplemented with OSO(interaction,P<0.01).Conclusions Inclusion of OSO intensified inflammatory states and impaired the intestinal health characteristics of weaned piglets.Dietary RES supplementation improved the antioxidant capacity,anti-inflammatory activity,and intestinal morphology.Further studies showed that the protective effects of RES on gut health could be linked to the decreased abundance of Prevotella_1,Clostridium_sensu_stricto_6,and Prevotellaceae_UCG003 and increased levels of acetic and propionic acid. | Yanan Gao Qingwei Meng Jianwei Qin Qianqian Zhao Baoming Shi | 2023 | Journal of Animal Science and Biotechnology2023，14，4： | 0 |
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