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| 1 | Induction of HLA-G expression in a melanoma cell line OCM-1A following the treatment with 5-aza-2'-deoxycytidine显示文摘The non-classical HLA class I antigen HLA-G is an immune modulator which inhibits the functions of T cells, NK cells, and the Dendritic cells (DC). As a result, HLA-G expression in malignant cells may provide them with a mechanism to escape the immune surveillance. In melanoma, HLA-G antigen expression has been found in 30% of surgically removed lesions but in less than 1% of established cell lines. One possible mechanism underlying the differential HLA-G expression in vivo and in vitro is that the HLA-G gene is epigenetically repressed in melanoma cells in vitro. To test this hypothesis, we treated the HLA-G negative melanoma cell line OCM-1A with the DNA methyltransferase inhibitor 5-aza-2’-deoXycytidine (5-AC) and analyzed whether HLA-G expression can be restored. Our data strongly suggest that HLA-G is silenced as a result of CpG hypermethylation within a 5’ regulatory region encompassing 220 bp upstream of the start codon. After treatment, HLA-G mRNA expression was dramatically increased. Western blot and flow cytometry showed that HLA-G protein was induced. Interestingly, HLA-G cell surface expression on the 5-AC treated OCM-1 A cells is much less than that on the HLA-G positive JEG-3 cells while a similar amount of total HLA-G was observed. Possible mechanisms for the difference were analyzed in the study such as cell cold-treatment, peptide loading and antigen processing machinery components (APM) as well as β2 microglobulin (β2-m) expression. Data revealed that the APM component calreticulin might be involved in the lower HLA-G surface expression on OCM-1 A cells. Taken together, our results indicated that DNA methylation is an important epigenetic mechanism by which HLA-G antigen expression is modulated in melanoma cells in vitro. Furthermore, to the first time, we hypothesized that the deficiency of calreticulin might be involved in the low HLA-G surface expression on the 5-AC treated OCM-1 A cells. | Wei Hua YAN Ai Fen LIN Chien Chung CHANG Soldano FERRONE | 2005 | Cell Research2005，15，7： | 5 |
| 2 | 靶向攻击肿瘤的免疫疗法显示文摘用单克隆抗体治疗癌症一直是肿瘤免疫学研究的热点之一。以往的抗体治疗均以癌细胞表面蛋白为靶标,因为全抗体分子太大而不能穿过细胞膜。最近新加坡科技研究局下属的分子与细胞生物学研究所(IMCB)曾琦博士及其团队的研究发现,抗体治疗不仅能以胞外癌蛋白为靶标,也能以胞内癌蛋白为靶标,进入胞内杀死癌细胞,从而抑制癌细胞的生长;这些发现对癌症的免疫治疗具有突破性的意义。现将其主要内容摘录如下,供我国学者参考。读者有意阅读原文请看:[Ferrone S.Hidden immunotherapy targets challenge dogma,Sci Transl Med,2011,3(99):99ps38] | Soldano Ferrone 江丽君 | 2012 | 微生物学免疫学进展2012，40，1： | 1 |
| 3 | Chemotherapy‐induced immunogenic modulation of tumor cells enhances killing by cytotoxic T lymphocytes and is distinct from immunogenic cell death显示文摘 | James W. Hodge Charlie T. Garnett Benedetto Farsaci Claudia Palena Kwong‐Yok Tsang Soldano Ferrone Sofia R. Gameiro | 2013 | Int J Cancer2013，，3： | 1 |
| 4 | NK cell activating ligands on human malignant cells: Molecular and functional defects and potential clinical relevance显示文摘 | Chien-Chung Chang Soldano Ferrone | 2006 | Seminars in Cancer Biology2006，，5： | 1 |
| 5 | Tumor Microenvironment and Immune Escape显示文摘 | Soldano Ferrone Theresa L. Whiteside | 2007 | Surgical Oncology Clinics of North America2007，，： | 1 |
| 6 | The complex role of B7 molecules in tumor immunology显示文摘 | Barbara Seliger Francesco M. Marincola Soldano Ferrone Hinrich Abken | 2008 | Trends in Molecular Medicine2008，，： | 1 |
| 7 | Association of antigen processing machinery and HLA class I defects with clinicopathological outcome in cervical carcinoma显示文摘 | Akash M. Mehta Ekaterina S. Jordanova Gemma G. Kenter Soldano Ferrone Gert- Jan Fleuren | 2008 | Cancer Immunology Immunotherapy2008，，2： | 1 |
| 8 | Effect of Honey and Eugenol on Ehrlich Ascites and Solid Carcinoma显示文摘 | Saravana Kumar Jaganathan Dilip Mondhe Z. A. Wani Harish C. Pal Mahitosh Mandal Soldano Ferrone | 2010 | Journal of Biomedicine and Biotechnology2010，，： | 1 |
| 9 | HLA-A2单抗轻链可变区基因的克隆及其序列分析显示文摘以HLA—A2单抗mRNA为模板,设计了3对特异性引物,采用PCR技术,扩增和克隆出单抗轻链可变区基因并应用双脱氧链式终止法及计算机基因文库测定和分析了其氨基酸和核苷酸序列。结果证明,本组单抗轻链由不同的基因家族编码。 | 苏娜 Elena A Armandola Soldano Ferrone | 1995 | 中华微生物学和免疫学杂志1995，15，5： | 0 |
| 10 | 癌症的单克隆抗体免疫治疗显示文摘单克隆抗体是许多恶性疾病的有效治疗手段。然而,与抗体的其他作用机制相比,抗体诱发肿瘤抗原特异性免疫应答的能力很少受到关注。本文收集相关证据,对研制可诱发宿主产生肿瘤抗原特异性免疫应答的抗体的合理性进行探讨。通过诱导抗体依赖性细胞毒性效应、刺激抗体靶向的肿瘤抗原交叉提呈或触发独特型网络,均可诱导该类应答。而改进治疗方式或实施联合治疗,将有可能延长、放大或调节这类免疫应答,并提高癌症抗体治疗的临床效果。 | Louis M Weiner Madhav V Dhodapkar Soldano Ferrone 王顺涛(译) | 2009 | 世界临床医学2009，，11： | 0 |
| 11 | 口腔鳞癌细胞系HLAI类分子异常表达及其机制探讨显示文摘为探讨两个口腔鳞癌细胞系 (KB和Tca81 1 3)中HLAI类抗原的表达水平及其异常的分子机制。利用流式细胞术、Westernblot检测细胞系中HLAI类抗原在蛋白水平的表达 ;RT PCR检测细胞系在转录水平的表达。结果两个口腔鳞癌细胞系中HLAI类抗原蛋白水平的表达下调 ;RT PCR结果显示Tca81 1 3细胞系中A、B、C、LMP2、LMP7、LMP1 0基因的mRNA表达下调 ;在KB细胞系中除了A、B、C、LMP7、LMP1 0基因的mRNA表达下调 ,LMP2、PA2 8α基因的mRNA表达显著减少或缺失 ;而TAP1、TAP2、Tapasin基因在mRNA水平的表达无改变。IFN γ诱导后纠正了多个基因在mRNA水平的异常表达。两个口腔鳞癌细胞系中HLAI类抗原表达下调 。 | 唐秋莎 张建琼 祁兵 沈传来 鲁晓萱 鲍海逊 Soldano Ferrone Xinhui Wang 谢维 | 2003 | 上海免疫学杂志2003，23，4： | 0 |
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