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| 1 | Induction of HLA-G expression in a melanoma cell line OCM-1A following the treatment with 5-aza-2'-deoxycytidine显示文摘The non-classical HLA class I antigen HLA-G is an immune modulator which inhibits the functions of T cells, NK cells, and the Dendritic cells (DC). As a result, HLA-G expression in malignant cells may provide them with a mechanism to escape the immune surveillance. In melanoma, HLA-G antigen expression has been found in 30% of surgically removed lesions but in less than 1% of established cell lines. One possible mechanism underlying the differential HLA-G expression in vivo and in vitro is that the HLA-G gene is epigenetically repressed in melanoma cells in vitro. To test this hypothesis, we treated the HLA-G negative melanoma cell line OCM-1A with the DNA methyltransferase inhibitor 5-aza-2’-deoXycytidine (5-AC) and analyzed whether HLA-G expression can be restored. Our data strongly suggest that HLA-G is silenced as a result of CpG hypermethylation within a 5’ regulatory region encompassing 220 bp upstream of the start codon. After treatment, HLA-G mRNA expression was dramatically increased. Western blot and flow cytometry showed that HLA-G protein was induced. Interestingly, HLA-G cell surface expression on the 5-AC treated OCM-1 A cells is much less than that on the HLA-G positive JEG-3 cells while a similar amount of total HLA-G was observed. Possible mechanisms for the difference were analyzed in the study such as cell cold-treatment, peptide loading and antigen processing machinery components (APM) as well as β2 microglobulin (β2-m) expression. Data revealed that the APM component calreticulin might be involved in the lower HLA-G surface expression on OCM-1 A cells. Taken together, our results indicated that DNA methylation is an important epigenetic mechanism by which HLA-G antigen expression is modulated in melanoma cells in vitro. Furthermore, to the first time, we hypothesized that the deficiency of calreticulin might be involved in the low HLA-G surface expression on the 5-AC treated OCM-1 A cells. | Wei Hua YAN Ai Fen LIN Chien Chung CHANG Soldano FERRONE | 2005 | Cell Research2005，15，7： | 5 |
| 2 | 运用无桥式整流电路实现高效率的PFC设计显示文摘 | Ron Brown Marco Soldano | 2005 | 电子设计应用2005，，6： | 2 |
| 3 | Optical Multi-Mode Interference Devices Based on Self-Imaging:Principles and Applications显示文摘 | Soldano L B Pennings E C M | 1995 | J Lightwave Technol1995，13，： | 1 |
| 4 | Production, properties and potential of graphene显示文摘 | Soldano C Mahmood A Dujardin E | 2010 | Carbon2010，48，8： | 1 |
| 5 | Optical multi- mode interfer- ence devices based on selfimaging: Principles and applica- tions显示文摘 | Soldano L Pennings E C M | 1995 | Journal of Lightwave Technology1995，13，： | 1 |
| 6 | Optical multi-mode inter- ference devices based on selfimaging:Prineiples and appli- cations 显示文摘 | SOLDANO L PENNINGS E C M | 1995 | Journal of Lightwave Technology1995，13，4： | 1 |
| 7 | Ultracompact,Low-Loss Directional Couplers on InP Based on Self-Imaging by Multimode Interference显示文摘 | Pennings E C M Deri R J Scherer A Bhat R Hayes T R Andreadakis N C Smit M K Soldano L B Hawkins R J | 1991 | Appl Phys Lett1991，59，： | 1 |
| 8 | Optical multi- mode interference devices based on self- imaging principles and application显示文摘 | Lucas B. Soldano Erik C. M. Pennings | | 0，，04： | 1 |
| 9 | Optical multi-mode interference devices based on self-imaging:Principles and applications显示文摘 | Soldano L B Pennings E C M | 1995 | J Lightwave Technol1995，13，4： | 1 |
| 10 | Eruptive xanthomas显示文摘 | Merola JF Mengden S J Soldano A | 2008 | Dermatol Online J2008，14，5： | 1 |
| 11 | Cutaneous solitary fibrous tumor:a report of 2 cases and review of the literature显示文摘 | Soldano AC Meehan SA | | 0，，01： | 1 |
| 12 | 靶向攻击肿瘤的免疫疗法显示文摘用单克隆抗体治疗癌症一直是肿瘤免疫学研究的热点之一。以往的抗体治疗均以癌细胞表面蛋白为靶标,因为全抗体分子太大而不能穿过细胞膜。最近新加坡科技研究局下属的分子与细胞生物学研究所(IMCB)曾琦博士及其团队的研究发现,抗体治疗不仅能以胞外癌蛋白为靶标,也能以胞内癌蛋白为靶标,进入胞内杀死癌细胞,从而抑制癌细胞的生长;这些发现对癌症的免疫治疗具有突破性的意义。现将其主要内容摘录如下,供我国学者参考。读者有意阅读原文请看:[Ferrone S.Hidden immunotherapy targets challenge dogma,Sci Transl Med,2011,3(99):99ps38] | Soldano Ferrone 江丽君 | 2012 | 微生物学免疫学进展2012，40，1： | 1 |
| 13 | Multifunctional composites using reinforced laminaewith carbon-nanotube forests 显示文摘 | VeeduVP CaoA LiX MAK Soldano C Kar S etal | 2006 | Nat Mater2006，5，： | 1 |
| 14 | Influence of passive leg elevation on the right ventricular function in anaesthetized coronary patients显示文摘 | Bertolissi M Broi UD Soldano F | 2003 | Crit Care2003，7，2： | 1 |
| 15 | Optical Multi-Mode Interference Device Based on Self-Imaging:Principles and Applications显示文摘 | Soldano Lacas B Pennings Erik C M | 1995 | Journal of Lightwave Techmolegy1995，13，4： | 1 |
| 16 | Optical Multi-mode interference devices based on self-imaging:Principles and Applications显示文摘 | Soldano L B Pennings E C M | 1995 | IEEE J Lightwave Technol1995，13，： | 1 |
| 17 | Beyond pathology: APP, brain develop- ment and Alzheimer's disease 显示文摘 | Soldano A Hassan BA | 2014 | Curt Opin Neurobiol2014，27，6： | 1 |
| 18 | Subcutaneous ossifying fibromyxoid tumor显示文摘 | Soldano A C Vazques-Martui E Romero J A | 2006 | J Cutan Pathol2006，33，： | 1 |
| 19 | Production, prop- erties and potential of grapheme 显示文摘 | Soldano C Mahmood A Dujardin E | 2010 | Carbon2010，，48： | 1 |
| 20 | Optical multi-mode interference devices based on self-imaging: principles and applications显示文摘 | SOLDANO L B PENNINGS E C M | 1995 | IEEEJ Lightwave Technol1995，13，61： | 1 |
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