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| 1 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 2 | Wear behavior of triode-sputtered MoS2 coatings in dry sliding contact with steel and ceramics 显示文摘 | Singer I L Fayulle S | 1996 | Wear1996，195，3： | 1 |
| 3 | Wear behavior of triode-sputtered MoS2 coatings in dry sliding contact with steel and ceramics显示文摘 | Singer I L Fayulle S | 1996 | Wear1996，195，3： | 1 |
| 4 | An endothelial ligand for L - selection is a novel mucin - like molecule 显示文摘 | LASKY L A SINGER M S DOWBENKO D | 1992 | Cell1992，69，： | 1 |
| 5 | Effect of topiramate on body weight and body composition of oborne-mendel rats fed a high-fat diet:alterations in hormones,neuropeptide and uncoupling-protein mRNAs显示文摘 | York DA Singer L Thomas S | 2000 | Nutrition2000，16，： | 1 |
| 6 | Characterization of Pico Green reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation显示文摘 | SINGER V L JONES L J YUE S T | 1997 | Anal Biochem1997，249，2： | 1 |
| 7 | Studies on the respiratory chain-linked dihydrodiphosphopyridine nucleotide dehydrogenase 显示文摘 | MINAKAMI S RINGLER R L SINGER T P | 1962 | Biol Chem1962，237，2： | 1 |
| 8 | Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double--stranded DNA quantitation显示文摘 | Singer V L Jones L J Yue S T | 1997 | Analytical Biochemistry1997，249，2： | 1 |
| 9 | Conjunctival anaerobic and aerobic bacterial flora in paediatric versus adult subjects显示文摘 | Singer TR Isenberg S J Apt L | 1988 | Br J Ophthalmol1988，72，6： | 1 |
| 10 | The fluid mosaic model of the structure of cell membranes 显示文摘 | Singer S J Nicolson G L | 1972 | Science1972，175，23： | 1 |
| 11 | Second harmonic generation in poled polymer films显示文摘 | SINGER K D SOHN J E LALAMA S L | 1986 | Appl Phys Let1986，49，： | 1 |
| 12 | Effect of topiramate on body weight and body composition of osborne-mendel rats fed a high-fat diet: alterations in hormones, neuropep- tide, and uncoupling-protein mRNAs显示文摘 | York DA Singer L Thomas S | 2000 | Nutrition2000，16，10： | 1 |
| 13 | Triclosan :occurrence and fateof a widely used biocide in the aquatic environment: Field measurements in wastewater treatment plants, surface waters, and lake sediments 显示文摘 | Singer H S Muller C Tixier PilIonel L | 2002 | Environ Sci Technol2002，36，23： | 1 |
| 14 | Comparison of the 3ormethod and time-domain thermo reflectance for measurements of the cross- plane thermal conductivity of epitaxial semiconductors显示文摘 | Koh Y K Singer S L Kim W | 2009 | J Appl Phys2009，105，05： | 1 |
| 15 | Corpus callosum morphology in children with Tourette syndrome and attention deficit hyperactivity disorder显示文摘 | Singer H S Denckla M B | 1996 | Neurology1996，47，2： | 1 |
| 16 | Use of the Intemet and E-mail for Health Care Information: Results From a National Survey显示文摘 | Baker L Wagner TH Singer S et M | 2003 | J A M ( S0098 - 7484 )2003，289，18： | 1 |
| 17 | Optimization of analytical parameters for inferring relationships among Escherichia coli isolates from repetitive-element PCR by maximizing correspondence with multilocus sequence typing data显示文摘 | Goldberg T L Gillespie T R Singer R S | | 0，，09： | 1 |
| 18 | Triclosan:Occurrence and fate of a widely used biocide in the aquatic environment:Field measurements in wastewater treatment plants,surface waters,and lake sediments显示文摘 | Singer H Mül er S Tixier C | 2002 | Environmental Science&Technology2002，36，23： | 1 |
| 19 | Quality of life improvement after hospital-based rehabilitation or home-based physical training in intermittent claudieation显示文摘 | Imfeld S Singer L Degischer S | 2006 | VASA2006，35，3： | 1 |
| 20 | Comparison of the 3 Omega Method and Time-Domain Thermorefiectance for Measurements of the Cross-Plane Thermal Conduc- tivity of Epitaxial Semiconductors 显示文摘 | Koh Y K Singer S L Kim W | 2009 | J Appl Phys2009，105，05： | 1 |
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