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| 1 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 2 | 伦理学与SARS:多伦多的教训显示文摘SARS的流行说明传染病在世界范围内流行是件多么容易的事,为了更好地应对下一次流行病,我们不仅要解决医学方面的问题,也同样需要解决伦理学方面的问题。 | Peter A Singer Solomon R Benatar Mark Bernstein Abdallah S Daar Bernard M Dickens Susan K MacRae Ross E G Upshur Linda Wright Randi Zlotnik Shaul 肖月 | 2004 | 英国医学杂志中文版2004，7，4： | 3 |
| 3 | In vitro antimicrobial activity against 10 North American and European Lawsonia intracellularis isolates显示文摘 | WATTANAPHANSAK S SINGER R S GEBHART C J | 2009 | Vet Microbiol2009，134，34： | 1 |
| 4 | Psyehosocial aftercare of patients with endometrial or cervical cancer显示文摘 | Singer S Schwarz R | 2002 | Zentralbl Gynakol2002，124，1： | 1 |
| 5 | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent 显示文摘 | Zolfaghari PS Packer S Singer M | 2009 | BMC Microbiol2009，9，： | 1 |
| 6 | Carbon fibres from mesophase pitch显示文摘 | LEONARD S SINGER | 1981 | Fuel1981，60，9： | 1 |
| 7 | Wear behavior of triode-sputtered MoS2 coatings in dry sliding contact with steel and ceramics 显示文摘 | Singer I L Fayulle S | 1996 | Wear1996，195，3： | 1 |
| 8 | Assessment of the clinical effectiveness of pulmonary artery catheters in management of patients in intensive care ( PAC-Man ) : a randomised controlled trial 显示文摘 | Harvey S Harrison DA Singer M | 2005 | Lancet2005，366，9484： | 1 |
| 9 | Understanding consumption-related sucralose emissions - A conceptual approach combining substance-flow analysis with sampling analysis 显示文摘 | Neset T S Singer H Longree P | 2010 | Sci Total Environ2010，408，16： | 1 |
| 10 | Occurrence and fate ofcarbamazepine, clofibric acid, diclofenac, ibuprofen, ketoprofen, and naproxen in surface waters 显示文摘 | Tixier C Singer H P Oellers S | 2003 | Environmental Science & Technology2003，37，6： | 1 |
| 11 | Suharachnoid space disease : diagnosis with fluid - attenuated inversion - recovery MR imaging and comparison with gadolinium - enhanced spin - echo MR imaging: blinded reader study 显示文摘 | Singer M Atlas S Drayer B | 1998 | Radiology1998，208，2： | 1 |
| 12 | The ability of clove oil and MS-222 to minimize handling stress in rainbow trout ( Oncorhynchus mykiss Walbaum ) 显示文摘 | WAGNER G N SINGER T D MCKINLEY R S | 2003 | Aquaculture Research2003，34，： | 1 |
| 13 | Signal processing for underwater acoustic communications 显示文摘 | Singer A C Nelson J K Kozat S S | 2009 | 1EEE Com- munication Magazine2009，47，1： | 1 |
| 14 | On the equivalence of weak learn ability and linear reparability: New relaxations and efficient boosting algorithms 显示文摘 | Shalev Shwartz S Singer Y | 2010 | Machine learning2010，80，23： | 1 |
| 15 | Magnetic resonance observation of cartilage repair tissue(MOCART)for the evaluation of autologous chondrocyte transplantation:determination of interobserver variability and correlation to clinical outcome after 2 years显示文摘 | Marlovits S Singer P Zeller P | 2006 | Eur J Radiol2006，57，1： | 1 |
| 16 | Multiple effects of trehalose on protein folding in vitro and in vivo显示文摘 | Singer M A Lindquist S | 1998 | Mol Cell1998，1，5： | 1 |
| 17 | Esculin hydrolysis by Enterobacteriaceae 显示文摘 | Edberg SC Pittman S Singer JM | 1977 | J Clin Micrbiol1977，6，2： | 1 |
| 18 | Maternal psychological dis tress and parenting stress after the birth of a very low birth-weight infant显示文摘 | Singer LT Salvator A Guo S | 1999 | JAMA1999，281，9： | 1 |
| 19 | l-Iistologic sub- type and margin of resection predict pattern of recurrence and survival for retroperitoneal liposareoma 显示文摘 | SINGER S ANTONCSCU CR R1EDEL E | 2003 | Ann surg2003，238，3： | 1 |
| 20 | Continuous elevation of lung sound amplitudes, recorded at fixed flow rate, may indicate an increase in lung water content 显示文摘 | S Lev P Singer K Robinson | 2011 | Critical Care2011，15，1： | 1 |
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