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| 1 | Prospective evaluation of small bowel preparation with bisacodyl and sodium phosphate for capsule endoscopy显示文摘AIM: To determine the effect of Prepacol?, a com- bination of sodium phosphate and bisacodyl, on transit and quality of capsule endoscopy (CE). METHODS: Fivety two consecutive patients were included in this prospective study. CE was performed following a 12 h fasting period. Twenty six patients were randomized for additional preparation with Prepacol?. The quality of CE was assessed separately for the proximal and the distal small bowel by 3 experienced endoscopists on the basis of a graduation which was initially developed with 20 previous CE. RESULTS: Preparation with Prepacol? accelerated small bowel transit time (262 ± 55 min vs 287 ± 97 min), but had no effect on the quality of CE. Visibility was significantly reduced in the distal compared to the proximal small bowel. CONCLUSION: The significantly reduced visibility of CE in the distal small bowel allocates the need for a good preparation. Since Prepacol? has no beneficial effect on CE the modality of preparation and the ideal time of application remains unclear. Further standardized examinations are necessary to identify sufficient preparation procedures and to determine the impact of the volume of the preparation solution. | Andreas Franke Frank Hummel Phillip Knebel Christoph Antoni Ulrich Bcker Manfred V Singer Matthias Lhr | 2008 | World Journal of Gastroenterology2008，14，13： | 5 |
| 2 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 3 | Characterization of Pico Green reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation显示文摘 | SINGER V L JONES L J YUE S T | 1997 | Anal Biochem1997，249，2： | 1 |
| 4 | Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double--stranded DNA quantitation显示文摘 | Singer V L Jones L J Yue S T | 1997 | Analytical Biochemistry1997，249，2： | 1 |
| 5 | Comparison of SYBRGreen I nucleic acid gel stain mutagenicity and ethidi-um bromide mutagenicity in the Salmonella/mamma-lian microsome reverse mutation assay显示文摘 | Singer V L Lawlor T E | 1999 | MutationResearch/Genetic Toxicology and Environmental Mu-tagenesis1999，439，1： | 1 |
| 6 | Adrenal function in sepsis: The retrospective corticus cohort study显示文摘 | Lipiner-Friedman D Sprung C L Laterre P F Weiss Y Goodman S V Vogeser M Briegel J Keh D Singer M Moreno R Bellissant E Annane D | 2007 | Critical Care Medicine2007，35，4： | 1 |
| 7 | Applications of SYPRO Orange and SYPRO Red proteingel stains 显示文摘 | Steinberg T H Haugtand R P Singer V L | 1996 | Anal Biochem1996，239，： | 1 |
| 8 | Comparison of SYBR Green I nucleic acid gel stain mutagenieity and ethidium bromide mutagenieity in the Salmonella/mammalian mierosome reverse mutation assay 显示文摘 | Singer V L Lawlor T E Yue S | 1999 | Mutation Research1999，439，1： | 1 |
| 9 | Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay显示文摘 | Singer V L Lawlor T E Yue S | 1999 | Mutat Res1999，439，1： | 1 |
| 10 | Comparison of SYBR Green Ⅰ nucieic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay显示文摘 | SINGER V L LAWLOR T E YUE S | 1999 | Mutat Res1999，439，1： | 1 |
| 11 | Geology and structural evolution of the Muruntau gold deposit,Kyzylkum desert,Uzbeklstan显示文摘 | Berkgaut V Singer A Drew L J | 1996 | Ore Geology Reviews1996，11，4： | 1 |
| 12 | Comparison of SYBR Green Ⅰ nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella mammalian microsome reverse mutation assay(Ames test)显示文摘 | Singer V L Lawlor T E Yue S | 1999 | Mutation Research1999，439，1： | 1 |
| 13 | Detection of measles vi rus nucleocapsid transcripts in circulation blood cells from patients with Paget disease显示文摘 | Reddy S V Singer F R Mallette L | 1996 | J Bone Miner Res1996，11，11： | 1 |
| 14 | Comparison of SYBR Green Inucle- ic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reversemutation assay (Ames test) 显示文摘 | Singer V L Lawlor T E Yue S | 1999 | Mutation Research1999，439，： | 1 |
| 15 | Characterization of picogreen reagent and development of a fluorescence-based solution assay for double-stranded D NA quantitation 显示文摘 | SINGER V L JONES L J YUE S T | 1997 | Anal Biochem1997，249，： | 1 |
| 16 | Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in theSalmonella/mammalian microsome reverse mutation assay (Ames test) 显示文摘 | Singer V L Lawlor T E Yue S | 1999 | Mutat Res1999，439，1： | 1 |
| 17 | The novel nucleoside analog R1479(4'-azidocytidine)is a potent inhibitor of NS5B-dependent RNA synthesis and hepatitis C virus replication in cell culture显示文摘 | Klumpp K Lévêque V Le Pogam S Ma H Jiang W R Kang H Granycome C Singer M Laxton | | 0，，07： | 1 |
| 18 | A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation显示文摘 | SINGER V L WOBBLE C R STRUHL K | 1990 | Genes & Dev1990，4，： | 1 |
| 19 | Comparison of SYBR Green I nucleic acid gel stain mutagenieity and ethidium bromide mutagenieity in the Salmonella/mammalian microsome reverse mutation assay 显示文摘 | Singer V L Lawlor T E Yue S | 1999 | Murat Res1999，439，： | 1 |
| 20 | A high-resolution, fluorescence-based method for localization of endogenous alkaline phnsphatase activity 显示文摘 | Cox W G Singer V L | 1999 | J Histochem Cytochem1999，47，11： | 1 |
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