|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis显示文摘AIM: To evaluate the effect of pyrrolidine dithio- carbamate (PDTC; an NF-κB inhibitor) administered at low (50 mg/kg) and high (100 mg/kg) doses in suppressing colitis in mice with dextran sodium sulfate (DSS)-induced colitis. METHODS: Mice were divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+PDTC-treated groupⅠ(low-dose group), and DSS+PDTC-treated groupⅡ (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue were measured. RESULTS: The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels were significantly lower in DSS+PDTC- treated groupⅡ. CONCLUSION: These findings suggest that PDTC is useful for the treatment of ulcerative colitis. | Ichiro Hirata Shingo Yasumoto Ken Toshina Takuya Inoue Takashi Nishikawa Naoko Murano Mitsuyuki Murano Fang-Yu Wang Ken-ichi Katsu | 2007 | World Journal of Gastroenterology2007，13，11： | 20 |
| 2 | Comparative study of esomeprazole and lansoprazole in triple therapy for eradication of Helicobacter pylori in Japan显示文摘AIM:To evaluate the efficacy and safety of esomeprazole-based triple therapy compared with lansoprazole therapy as first-line eradication therapy for patients with Helicobacter pylori(H.pylori)in usual post-marketing use in Japan,where the clarithromycin(CAM)resistance rate is 30%.METHODS:For this multicenter,randomized,openlabel,non-inferiority trial,we recruited patients(≥20years of age)with H.pylori infection from 20 hospitals in Japan.We randomly allocated patients to esomeprazole therapy(esomeprazole 20 mg,CAM 400 mg,amoxicillin(AC)750 mg for the first 7 d,with all drugs given twice daily)or lansoprazole therapy(lansoprazole30 mg,CAM 400 mg,AC 750 mg for the first 7 d,with all drugs given twice daily)using a minimization method with age,sex,and institution as adjustment factors.Our primary outcome was the eradication rate by intention-to-treat(ITT)and per-protocol(PP)analyses.H.pylori eradication was confirmed by a urea breath test from 4 to 8 wk after cessation of therapy.RESULTS:ITT analysis revealed the eradication rates of 69.4%(95%CI:61.2%-76.6%)for esomeprazole therapy and 73.9%(95%CI:65.9%-80.6%)for lansoprazole therapy(P=0.4982).PP analysis showed eradication rate of 76.9%(95%CI:68.6%-83.5%)for esomeprazole therapy and 79.8%(95%CI:71.9%-86.0%)for lansoprazole therapy(P=0.6423).There were no differences in adverse effects between the two therapies.CONCLUSION:Esomeprazole showed non-inferiority and safety in a 7 day-triple therapy for eradication of H.pylori compared with lansoprazole. | Tsutomu Nishida Masahiko Tsujii Hirohisa Tanimura Shusaku Tsutsui Shingo Tsuji Akira Takeda Atsuo Inoue Hiroyuki Fukui Toshiyuki Yoshio Osamu Kishida Hiroyuki Ogawa Masahide Oshita Ichizo Kobayashi Shinichiro Zushi Makoto Ichiba Naoto Uenoyama Yuichi Yasunaga Ryu Ishihara Mamoru Yura Masato Komori Satoshi Egawa Hideki Iijima Tetsuo Takehara | 2014 | World Journal of Gastroenterology2014，20，15： | 13 |
| 3 | miR-122-5p as a novel biomarker for alpha-fetoproteinproducing gastric cancer显示文摘AIM To investigate the clinical utility of alpha-fetoprotein(AFP)-producing gastric cancer(AFPGC)-specific microRNA(mi RNA)for monitoring and prognostic prediction of patients.METHODS We performed a comprehensive miRNA array-based approach to compare miRNA expression levels between AFP-positive and AFP-negative cells in three patients with primary AFPGC.We next examined the expression levels of the selected miRNAs in five AFPGC and ten non-AFPGC tissue samples by quantitative reverse transcription-polymerase chain reaction to validate their utility.We also investigated the expression levels of the selected miRNA not only in tissue but also in plasma samples.Moreover,we investigated the relationship between plasma AFP levels and plasma selected miRNA expression levels,and also investigated the correlation of the selected miRNA expression levels and malignant potential.RESULTS Among the five miRNAs selected from the miRNA array results,the expression levels of miR-122-5p were significantly higher in the AFPGC patients than in the non-AFPGC patients(P<0.05).In tissue samples,mi R-122-5p expression level tended to be lower in the non-AFPGC tissue than the normal gastric mucosa.Conversely,in the AFPGC tissue,miR-122-5p expression level was significantly higher in the AFPGC tissue than both the normal gastric mucosa and the nonAFPGC tissue samples(P<0.05).Plasma mi R-122-5p expression levels were also significantly higher in the AFPGC patients than the health volunteers and the nonAFPGC patients(P<0.05)and were strongly correlated with plasma AFP levels(r=0.7975,P<0.0001).Moreover,the correlation of miR-122-5p expression in tissue samples with malignant potential was stronger than that of plasma AFP level in the AFPGC patients.In contrast,no correlation was found between mi R-122-5p expression levels and liver metastasis in the non-AFPGC patients.CONCLUSION miR-122-5p might be a useful biomarker for early detection and disease monitoring in AFPGC. | Suguru Maruyama Shinji Furuya Kensuke Shiraishi Hiroki Shimizu Hidenori Akaike Naohiro Hosomura Yoshihiko Kawaguchi Hidetake Amemiya Hiromichi Kawaida Makoto Sudo Shingo Inoue Hiroshi Kono Daisuke Ichikawa | 2018 | World Journal of Gastrointestinal Oncology2018，10，10： | 6 |
| 4 | Applicability of second‐generation colon capsule endoscope to ulcerative colitis: A clinical feasibility study显示文摘 | Naoki Hosoe Katsuyoshi Matsuoka Makoto Naganuma Yosuke Ida Yuka Ishibashi Kayoko Kimura Kazuaki Yoneno Shingo Usui Kazuhiro Kashiwagi Tadakazu Hisamatsu Nagamu Inoue Takanori Kanai Hiroyuki Imaeda Haruhiko Ogata Toshifumi Hibi | 2013 | J Gastroenterol Hepatol2013，，7： | 3 |
| 5 | Dieta- ry soybean protein moderates the deleterious disturbance of lipid metabolism caused by exogenous oxidized cholesterol in rats 显示文摘 | Kyoichi Osada Tomoko Inoue Shingo Nakamura | 1999 | Bioehimica et Biophysiea Acta1999，1427，3： | 1 |
| 6 | Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells显示文摘In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces'chloroplast de-anchoring', a rapid decline in the resistance of chloroplasts against centrifugal force. Chloroplast de-anchoring is known induced within 1 min of irradiation with high-fluence-rate blue light specifically, preceding the commencement of chloroplasts migration toward the anticlinal cytoplasm. However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response. In search of the responsible photoreceptors,immunoblotting analysis using antibodies against phototropins demonstrated that cross-reactive polypeptides of 120-k Da exist in the plasma-membrane fraction prepared from the leaves. In vitro phosphorylation analysis revealed that 120-k Da polypeptides were phosphorylated by exposure to blue light in a fluence-dependent manner. The blue-light-induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness. Furthermore, phototropin gene homologs(Vallisneria PHOTOTROPIN1 and PHOTOTROPIN2)expressed in leaves were isolated. We propose that calcium-regulated chloroplast de-anchoring, possibly mediated by phototropins, is an initial process of the blue-light-induced avoidance response of chloroplasts in Vallisneria. | Yuuki Sakai Shin-Ichiro Inoue Akiko Harada Ken-Ichiro Shimazaki Shingo Takagi | 2015 | Journal of Integrative Plant Biology2015，57，1： | 1 |
| 7 | Refractory ulcerative colitis accompanied with cytomegalovirus colitis and multiple liver abscesses: A case report显示文摘Various hepato-biliary complications are an increased incidence in patients with inflammatory bowel disease,and portal bacteremia is well documented in patients with ulcerative colitis (UC). However, few reports mention UC in association with liver abscesses. Recently, there are several reports describing cytomegalovirus (CMV) infection in association with disease exacerbation and steroid refractoriness in patients with UC. Here we present a case of refractory UC accompanied with multiple liver abscesses and CMV colitis. The patient, a 72-year-old male, with a five-year history of repeated admissions to our hospital for UC, presented with an exacerbation of his UC.Sigmoidoscopy performed on admission suggested that his UC was exacerbated, then he was given prednisolone and mesalazine orally, and betamethasone enemas.However, he had exacerbated symptoms. Repeat sigmoidoscopy revealed multiple longitudinal ulcers and pseudopolyps in the rectosigmoid colon. Although immunohistochemical staining of biopsy specimens and the serum testing for antigenemia were negative on admission and after the repeat sigmoidoscopy, they became histologically positive for CMV. Nonetheless, the patient developed spiking fevers, soon after ganciclovir was administered. Laboratory studies revealed an increased white cell count with left shift, and Enterococcus fecalis grew in blood cultures. An abdominal computed tomography (CT) scan was obtained and the diagnosis of liver abscesses associated with UC was made, based on CT results. The hepatic abscesses were successfully treated with intravenous meropenem for 6 wk, without further percutaneous drainage. To our knowledge, this is the first reported case of multiple liver abscesses that develop during UC exacerbation complicated by CMV colitis. | Takuya Inoue Ichiro Hirata Yutaro Egashira Kumi Ishida Ken Kawakami Eijiro Morita Naoko Murano Shingo Yasumoto Mitsuyuki Murano Ken Toshina Takashi Nishikawa Norihiro Hamamoto Ken Nakagawa Ken-Ichi Katsu | 2005 | World Journal of Gastroenterology2005，11，33： | 1 |
| 8 | Development and evaluation of a formalin-inactivated West Nile Virus vaccine (WN-VAX) for a human vaccine candidate显示文摘 | Guillermo Posadas-Herrera Shingo Inoue Isao Fuke Yuko Muraki Cynthia A. Mapua Afjal Hossain Khan Maria del Carmen Parquet Sadao Manabe Osamu Tanishita Toyokazu Ishikawa Filipinas F. Natividad Yoshinobu Okuno Futoshi Hasebe Kouichi Morita | 2010 | Vaccine2010，，50： | 1 |
| 9 | INHIBITION OF NITRIC OXIDE SYNTHESIS AGGRAVATES MYOCARDIAL ISCHEMIA IN HEMORRHAGIC SHOCK IN CONSTANT PRESSURE MODEL显示文摘 | Takeshi Adachi Shingo Hori Koji Miyazaki Masahiro Nakagawa Soshin Inoue Yozo Ohnishi Hiroe Nakazawa Naoki Aikawa Satoshi Ogawa | 1998 | Shock1998，，3： | 1 |
| 10 | Real- time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile Virus 显示文摘 | Manmohan Parida Guillermo Posadas Shingo Inoue | 2004 | J Clin Microbiol2004，42，1： | 1 |
| 11 | Effects of injection angle of slotted nozzle on supersmlic mixing显示文摘 | Kei Inoue Shigeru kso Shingo Kawano | 2004 | Computational Fluid Dy- namics Journal2004，13，2： | 1 |
| 12 | Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples显示文摘为了评估从 HIV-1 的精液为人的免疫不全病毒类型 1 的移动( HIV-1 )包括 Percoll 连续密度坡度和修改游泳起来技术的联合的一个过程的临床的功效,感染了男性, 129 的一个总数结合 HIV-1 积极男搭挡和 HIV-1 在在2002年1月和2012年4月之间的 Keio 大学医院被对待的否定女搭挡( serodiscordant 夫妇)被检查。从 129 HIV-1 的 183 精液的一个总数感染了男性被处理。在游泳起来以后,我们成功地在恢复收集了能动精子在 normozoospermia (126/126 精液) 的情况下象 100.0% 一样高的率, oligozoospermia (6/6 ) ,和 asthenozoospermia (36/36 ) 。oligoasthenozoospermia 的恢复率是 86.7%(13/15 ) 。在处理精液,仅仅四精液(4/181:2.2%) 在感染的男性的血显示出与那些一致的病毒的核苷酸序列。在使用这些精子以后,女病人的水平感染和新生儿的没有垂直感染都没被观察。而且,没有明显的不利效果在后代被观察。这个协议允许我们收集在高度的否定能动精子评估的 HIV-1,甚至在男因素盒子中。我们断定我们的协议为减少的 HIV-1 感染并且为产出一个健康孩子是临床上有效的。 | Osamu Inoue Naoaki Kuji Hiroe Ito Mitsutoshi Yamada Toshio Hamatani Aimi Oyadomari Shingo Kato Hideji Hanabusa Keiichi Isaka Mamoru Tanaka | 2017 | Asian Journal of Andrology2017，19，2： | 0 |
| 13 | 日本脑炎ML-17两个变异株的基因组序列及突变分析(英文)显示文摘目的鉴别日本脑炎疫苗株ML-17的两个大宽变异株(ML-17L2和ML-17L4)的存在,并通过全基因组序列测定分析引起斑形和毒力改变的基因突变。方法首先对疫苗株ML-17在传代过程中出现的多斑ML-17sh的两个大宽变异株进行纯化和鉴定,再对两个大斑变异株进行全基因组序列测定和全编码氨基酸序列推导。并与小斑疫苗株ML-17及其大斑亲代野毒株JaOH0566的全核苷酸序列和全编码氨基酸序列进行比较,分析引起宽形改变的基因突变。并应用小白鼠试验检查ML-17变异前后的神经侵袭力和毒力变化。结果全基因组序列分析显示两个大斑变异株的核苷酸总数均为10977个,与ML-17和JaOH0566相比较,分剐有33和34个核苷酸变异散在分布于ML-17L2和ML-17L4的全基因组中,其中又分别有12和13个核苷酸导致了氨基酸的改变。在ML-17L2和ML-17L4的衣壳蛋白区没有氨基酸变异,但在包膜蛋白区各有一个氨基酸变异;在5’端非编码区没有核苷酸变异,但在3’端非编码区有3~4个核苷酸变异,且各增加了一个额外的10699核苷酸。ML-17和ML-17sh的小鼠LD50分别为大于1×10^6FFU和481FFU。变异后毒力明显增强。结论发生在两个大斑变异株的第5非结构蛋白区和其它区的氨基酸返祖变异(变回与JaOH0566相同)和新变异,可能引起了宽彤的改变;而全部5个氨基酸返祖变异和2—3个氨基酸新变异可能与其毒力改变有关。本次研究为ML-17L2和ML-17L4的斑形和毒力改变提供了分子遗传学基础。 | 金玉明 Afjal Hossain Khan Mohammed Alimul Islam Takeshi Nabeshima Shingo Inoue Kouichi Morita | 2006 | 中国热带医学2006，6，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员