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| 1 | Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice显示文摘Drought is a major factor restricting the production of rice(Oryza sativa L.).The identification of natural variants for drought stress-related genes is an important step toward developing genetically improved rice varieties.Here,we characterized a member of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)family,OsSPL10,as a transcription factor involved in the regulation of drought tolerance in rice.OsSPL10 appears to play a vital role in drought tolerance by controlling reactive oxygen species(ROS)production and stomatal movements.Haplotype and allele frequency analyses of OsSPL10 indicated that most upland rice and improved lowland rice varieties harbor the OsSPL10Hap1allele,whereas the OsSPL10Hap2allele was mainly present in lowland and landrace rice varieties.Importantly,we demonstrated that the varieties with the OsSPL10Hap1allele showed low expression levels of OsSPL10 and its downstream gene,OsNAC2,which decreases the expression of OsAP37 and increases the expression of OsCOX11,thus preventing ROS accumulation and programmed cell death(PCD).Furthermore,the knockdown or knockout of OsSPL10 induced fast stomatal closure and prevented water loss,thereby improving drought tolerance in rice.Based on these observations,we propose that OsSPL10 confers drought tolerance by regulating OsNAC2 expression and that OsSPL10Hap1could be a valuable haplotype for the genetic improvement of drought tolerance in rice. | Yingxiu Li Shichen Han Xingming Sun Najeeb Ullah Khan Qun Zhong Zhanying Zhang Hongliang Zhang Feng Ming Zichao Li Jinjie Li | 2023 | Journal of Integrative Plant Biology2023，65，4： | 2 |
| 2 | Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes显示文摘Trichomes function in plant defenses against biotic and abiotic stresses;examination of glabrous lines,which lack trichomes,has revealed key aspects of trichome development and function.Tests of allelism in 51 glabrous rice(Oryza sativa)accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice.Here,we report that OsSPL10 acts as a transcriptional regulator controlling trichome development.Haplotype and transient expression analyses revealed that variation in the approximately 700-bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD-17993.Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL-HL6 background.Plants with genotype OsSPL10^(WD-17993)/HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the glumes.HAIRY LEAF6(HL6)encodes another transcription factor that regulates trichome initiation and elongation,and OsSPL10 directly binds to the HL6 promoter to regulate its expression.Moreover,the transcript levels of auxin-related genes,such as OsYUCCA5 and OsPIN-FORMED1b,were altered in OsSPL10 overexpression and RNAi transgenic lines.Feeding tests using locusts(Locusta migratoria)demonstrated that non-glandular trichomes affect feeding by this herbivore.Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions. | Jinjie Li Bo Tang Yingxiu Li Chenguang Li Minjie Guo Haiyang Chen Shichen Han Jin Li Qijin Lou Wenqiang Sun Peng Wang Haifeng Guo Wei Ye Zhanying Zhang Hongliang Zhang Sibin Yu Long Zhang Zichao Li | 2021 | Journal of Integrative Plant Biology2021，63，8： | 2 |
| 3 | Expression and regulation of genes involved in the reserve starch biosynthesis pathway in hexaploid wheat (Triticum aestivum L.)显示文摘Reserve starch of cereal crop accounts for about 70%of grain endosperm and acts as an important human carbohydrate resource worldwide.Wheat reserve starch is synthesized by enzymatic machinery in endosperm cells.To identify genes involved in starch biosynthesis,we constructed 30 RNA-Seq libraries of 10 endosperm-development periods and performed expression and localization analyses.Of 166 endosperm-expressed homologs of starch biosynthesis-related genes,74 showed expression correlated with reserve starch accumulation,including 26 with expected subcellular distribution and higher expression than their isoforms.The key proteins SUS3,UGP1,cAGPase,and Bt1-3 formed the main metabolic pathway and contributed the major substrates for starch processing in amyloplasts.Important isoforms,key pathway proteins,and the main carbon flux toward starch formation in the reserve starch biosynthesis pathway were identified.Based on a coexpression analysis,a library of 425 transcription factors was produced to screen for common regulators.TaMYB44 had features of transcription factors and bound to TaSUT1,TaSSIIIa,TaBEIIa,TaISA1,and TaBEIIb promoters in yeast,suggesting that the gene is a pathway regulator.This study sheds light on understanding the mechanism of reserve starch biosynthesis and will be helpful for increasing starch content in wheat endosperm via biotechnological strategies. | Yunsong Gu Shichen Han Lin Chen Junyi Mu Luning Duan Yaxuan Li Yueming Yan Xiaohui Li | 2021 | The Crop Journal2021，9，2： | 1 |
| 4 | The influence of TiO2 · H2O gel on hydrothermal synthesis of SrTiO3 powders显示文摘 | Shichen g Zhang Yuexin Han Bingchen Chen | 2001 | Materials Letters2001，51，11： | 1 |
| 5 | Microstructures and properties of 2099 Al-Li alloy显示文摘 | Yi Lin Ziqiao Zheng Shichen Li Xiang Kong Ye Han | 2013 | Materials Characterization2013，，： | 1 |
| 6 | El Ni?o/Southern Oscillation during the 4.2 ka event recorded by growth rates of corals from the North South China Sea显示文摘The 4.2 ka event that occurred during the period from 4 500–3 900 a BP was characterized by cold and dry climates and resulted in the collapse of civilizations around the world. The cause of this climatic event, however, has been under debate. We collected four corals(Porites lutea) from Yongxing Island, Xisha Islands, South China Sea, dated them with the U-series method, and measured the annual coral growth rates using X-ray technology. The dating results showed that the coral growth ages were from 4 500–3 900 a BP, which coincide well with the period of the4.2 ka event. We then reconstructed annual sea surface temperature anomaly(SSTA) variations based on the coral growth rates. The growth rate-based SSTA results showed that the interdecadal SSTA from 4 500–3 900 a BP was lower than that during modern times(1961–2008 AD). A spectral analysis showed that the SSTA variations from4 500–3 900 a BP were under the influence of El Nino-Southern Oscillation(ENSO) activities. From 4 500–4 100 a BP, the climate exhibited La Nina-like conditions with weak ENSO intensity and relatively stable and lower SSTA amplitudes. From 4 100–3 900 a BP, the climate underwent a complicated period of ENSO variability and showed alternating El Nino-or La Nina-like conditions at interdecadal time scales and large SSTA amplitudes. We speculate that during the early and middle stages of the 4.2 ka event, the cold climate caused by weak ENSO activities largely weakened social productivity. Then, during the end stages of the 4.2 ka event, the repeated fluctuations in the ENSO intensity caused frequent extreme weather events, resulting in the collapse of civilizations worldwide. Thus, the new evidence obtained from our coral records suggests that the 4.2 ka event as well as the related collapse of civilizations were very likely driven by ENSO variability. | Shaohua Dang Kefu Yu Shichen Tao Tao Han Huiling Zhang Wei Jiang | 2020 | Acta Oceanologica Sinica2020，39，1： | 1 |
| 7 | A novel protein domain is important for photosystem Ⅱ complex assembly and photoautotrophic growth in angiosperms显示文摘Photosystem Ⅱ(PSⅡ)is a multi-subunit protein complex of the photosynthetic electron transport chain that is vital to photosynthesis.Although the structure,composition,and function of PSⅡ have been extensively studied,its biogenesis mechanism remains less understood.Thylakoid rhodanese-like(TROL)provides an anchor for leaf-type ferredoxin:NADP^(+)oxidoreductase.Here,we report the chacterizaton of a second type of TROL protein,TROL2,encoded by seed plant genomes whose function has not previously been reported.We show that TROL2 is a PSⅡ assembly cofactor with essential roles in the establishment of photoautotrophy.TROL2 contains a 45-amino-acid domain,termed the chlorotic lethal seedling(CLS)domain,that is both necessary and sufficient for TROL2 function in PSⅡ assembly and photoautotrophic growth.Phylogenetic analyses suggest that TROL2 may have arisen from ancestral TROL1 via gene duplication before the emergence of seed plants and acquired the CLS domain via evolution of the sequence encoding its N-terminal portion.We further reveal that TROL2(or CLS)forms an assembly cofactor complex with the intrinsic thylakoid membrane protein LOW PSⅡ ACCUMULATION2 and interacts with small PSⅡ subunits to facilitate PSⅡ complex assembly.Collectively,our study not only shows that TROL2(CLS)is essential for photoautotrophy in angiosperms but also reveals its mechanistic role in PSⅡ complex assembly,shedding light on the molecular and evolutionary mechanisms of photosynthetic complex assemblyin angiosperms. | Wei Li Jiangfan Guo Xue Han Xiaowen Da Kai Wang Hongfei Zhao Shi-Tang Huange Bosheng Li Hang He Ruirui Jiang Shichen Zhou Peng Yan Tao Chen Yi He Jiming Xu Yu Liu Yunrong Wu Huixia Shou Zhongchang Wu Chuanzao Mao Xiaorong Mo | 2023 | Molecular Plant2023，16，2： | 0 |
| 8 | Nitrogen addition promotes foliar litterfall and element return in a subtropical forest,southwestern China显示文摘Nitrogen deposition has a considerable impact on biogeochemical cycling in terrestrial ecosystems.However,how litter production and element return respond to N addition remains poorly understood in nitrogen-rich subtropical regions.In this study,a 4-year nitrogen addition experiment explored its eff ects on foliar litter production and carbon,nitrogen and phosphorus in a subtropical Michelia wilsonii forest.A clear seasonal pattern in foliar litterfall was observed,regardless of nitrogen treatments,with a peak in spring and a smaller one in autumn.Foliar litter increased with increasing nitrogen but did not aff ect litter carbon concentrations and often decreased nitrogen and phosphorous concentrations.The eff ect of nitrogen addition was dependent on time(month/year).Carbon,nitrogen and phosphorous return showed similar bimodal seasonal patterns.Nitrogen addition increased carbon and nitrogen return but did not aff ect phosphorous.Our results suggest that the addition of nitrogen stimulates carbon and nutrient return via litterfall. | Yue Zhang Shichen Xiong Chengming You Sining Liu Lixia Wang Li Zhang Han Li Bo Tan Yang Liu Zhenfeng Xu | 2023 | Journal of Forestry Research2023，34，4： | 0 |
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