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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Catatonia:Our current understanding of its diagnosis, treatment and pathophysiology显示文摘Catatonia is a psychomotor syndrome that has been reported to occur in more than 10% of patients with acute psychiatric illnesses. Two subtypes of the syndrome have been identified. Catatonia of the retarded type is characterized by immobility, mutism, staring, rigidity, and a host of other clinical signs. Excited catatonia is a less common presentation in which patients develop prolonged periods of psychomotor agitation. Once thought to be a subtype of schizophrenia, catatonia is now recognized to occur with a broad spectrum of medical and psychiatric illnesses, particularly affective disorders. In many cases, the catatonia must be treated before any underlying conditions can be accurately diagnosed. Most patients with the syndrome respond rapidly to low-dose benzodiazepines, but electroconvulsive therapy is occasionally required. Patients with longstanding catatonia or a diagnosis of schizophrenia may be less likely to respond. The pathobiology of catatonia is poorly understood, although abnormalities in gamma-aminobutyric acid and glutamate signaling have been suggested as causative factors. Because catatonia is common, highly treatable, and associated with significant morbidity and mortality if left untreated, physicians should maintain a high level of suspicion for this complex clinical syndrome. Since 1989, we have systematically assessed patients presenting to our psychiatry service with signs of retarded catatonia. In this paper, we present a review of the current literature on catatonia along with findings from the 220 cases we have assessed and treated. | Sean A Rasmussen Michael F Mazurek Patricia I Rosebush | 2016 | World Journal of Psychiatry2016，6，4： | 7 |
| 3 | The spectrum of neuromyelitis optica显示文摘 | Dean M Wingerchuk Vanda A Lennon Claudia F Lucchinetti Sean J Pittock Brian G Weinshenker | 2007 | Lancet Neurology2007，，9： | 3 |
| 4 | The stomach and intestines aparoxysm is tiny to encroach upon the surgery显示文摘 | Lauence F Yee M D Sean J | 1996 | Current Diagnosis&Treatment in Gastroentetology1996，196，： | 1 |
| 5 | Protection of oriented strandboard with borate显示文摘 | Sean T Brunette G CotéF | 1999 | Forest Products Journal1999，49，6： | 1 |
| 6 | Intimate relations-fact or fiction:An analysis of business to business relationships in IRISH companies显示文摘 | SEAN B TERESA B BRIAN F | 2001 | Irish Journal of Management2001，22，1： | 1 |
| 7 | Lower extremity joint kinetic responses to external resistance variations显示文摘 | Sean P F George J S | 2008 | J Appl Biomech2008，24，1： | 1 |
| 8 | Helmholtz equation - least -squares method for reconstructing the acoustic pressure field显示文摘 | Zhaoxi W and Sean F | 1997 | J Acoust Soc Am1997，102，4： | 1 |
| 9 | wu and Jingyou Yu Reconstructing interior acoustic pressure fields via Helmholtz equation least -squares method显示文摘 | Sean F | 1998 | J Acoust Soc Am1998，104，4： | 1 |
| 10 | Nerve growth factor and corneal wound healing in dogs 显示文摘 | HEUNG M W ELLISON B SEAN F | 2002 | Experimental EyeReasearch2002，80，5： | 1 |
| 11 | Stem cells,cancer,and cancer stem cells显示文摘 | Tannishtha Reya Sean J M Michael F C | 2001 | Nature2001，414，： | 1 |
| 12 | Protection of oriented strand board with borate显示文摘 | Sean T G Brunette F C6t~ | 1999 | Forest Products Journal1999，49，6： | 1 |
| 13 | Determining factors in the treataent choice of patients with hypertension with complications and sec- ondary hypertension显示文摘 | Sean Daniel F Robert R | 2009 | Int J Health Care Qual Assur2009，22，4： | 1 |
| 14 | Autonomous Control of Cell and Organ Size by CHICO, a Drosophila Homolog of Vertebrate IRS1–4显示文摘 | Ruth B?hni Juan Riesgo-Escovar Sean Oldham Walter Brogiolo Hugo Stocker Bernard F Andruss Kathy Beckingham Ernst Hafen | 1999 | Cell1999，，7： | 1 |
| 15 | Stem cells cancer, and cancer stem cells显示文摘 | TANNISHTHA R SEAN J M MICHAEL F C | 2001 | Nature2001，414，6859： | 1 |
| 16 | A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis显示文摘 | Vanda A Lennon Dean M Wingerchuk Thomas J Kryzer Sean J Pittock Claudia F Lucchinetti Kazuo Fujihara Ichiro Nakashima Brian G Weinshenker | 2004 | 2004 (9451)2004，，9451： | 1 |
| 17 | Polymeric Microelectromechanical Systems显示文摘 | Steven A S Sean M F Shize Q | 2000 | Analytical Chemistry2000，72，： | 1 |
| 18 | Stem cells,cancer, and cancer stem cells显示文摘 | Tannishtha R Sean J Michael F | 2001 | Nature2001，414，3： | 1 |
| 19 | Structural analysis,magnetic and electrical properties of samarium substituted lithium-nickel mixed ferrites显示文摘 | Muthafar F Al-Hilli Sean Li Kassim S Kassim | 2012 | J Magn Magn Mater2012，324，5： | 1 |
| 20 | Noise analysis of automotive alternators显示文摘 | Sean F Wu Eugene Rivin and Michael Disalvo | 1999 | SAE1999，，： | 1 |
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