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| 1 | 我国首次分离到基因Ⅰ型乙型脑炎病毒显示文摘目的 对新分离乙型脑炎病毒鉴定 ,了解病毒E基因区段的氨基酸序列特征及基因分型。方法 2 0 0 1年在上海市采集蚊虫标本 ,用组织培养的方法分离到 7株病毒。进行病毒一般生物学鉴定 ,对病毒PrM和E基因区段约 2 0 0 0nt进行了扩增、克隆、测序。应用ClustalX软件做碱基配对和比较分析 ,种系发生采用PHYLIP软件包分析。结果 7株病毒可以引起BHK 2 1细胞规律病变 ,病变时间为 6 0h。乳鼠脑内接种病毒后 72h死亡。所有新分离病毒与标准乙型脑炎病毒抗体阳性反应。用病毒PrM区段 (4 5 6~ 6 95位核苷酸 )进行的基因分型分析 ,新分离的 7株病毒属于基因Ⅰ型乙型脑炎病毒。以减毒活疫苗株SA14 14 2为标准 ,对 7株病毒的E基因区段进行分析 ,7株病毒之间核苷酸同源性在 97.7%以上 ,氨基酸同源性在 99.2 %以上 ;7株病毒与疫苗株SA14 14 2核苷酸差异在 12 .2 %左右 ,氨基酸差异在 2 .8%~ 3.2 %之间 ,共有 14个共同的氨基酸位点差异。结论 2 0 0 1年在上海采集的蚊虫中新分离的 7株病毒属于基因Ⅰ型乙型脑炎病毒 。 | 王环宇 付士红 李晓宇 宋宏 闵继光 邓娟 杨益良 Ichiro Kurane 梁国栋 | 2004 | 中华微生物学和免疫学杂志2004，24，11： | 79 |
| 2 | CONVERGENCE ANALYSIS FOR A NONCONFORMING MEMBRANE ELEMENT ON ANISOTROPIC MESHES显示文摘有限元素网孔的常规假设为常规一致元素 andnonconform-ing 元素两个都是有限元素近似的大多数分析的一个基本条件。这篇论文的目的是介绍处理一个四度的非一致的有限元素的近似的一条新奇途径因为第二在各向异性的网孔上订椭圆形的问题。没有在那里喉部的假设或伪制服假设的精力标准和 L2 标准的最佳的错误估计基于此处发现的这个元素的一些新特殊特征被获得。数字结果被给表明我们的理论分析的有效性。 | Dong-yang Shi Shao-chun Chen Ichiro Hagiwara | 2005 | Journal of Computational Mathematics2005，23，4： | 43 |
| 3 | Female hepatology:Favorable role of estrogen in chronic liver disease with hepatitis B virus infection显示文摘Chronic hepatitis B virus (HBV) infection is the most common cause of hepatic fibrosis and hepatocellular carcinoma (HCC),mainly as a result of chronic necroinflammatory liver disease. A characteristic feature of chronic hepatitis B infection,alcoholic liver disease and nonalcoholic fatty liver disease (NAFLD) is hepatic steatosis. Hepatic steatosis leads to an increase in lipid peroxidation in hepatocytes,which,in turn,activates hepatic stellate cells (HSCs). HSCs are the primary target cells for inflammatory and oxidative stimuli,and these cells produce extracellular matrix components. Chronic hepatitis B appears to progress more rapidly in males than in females,and NAFLD,cirrhosis and HCC are predominately diseases that tend to occur in men and postmenopausal women. Premenopausal women have lower hepatic iron stores and a decreased production of proinflammatory cytokines. Hepatic steatosis has been observed in aromatase-deficient mice,and has been shown to decrease in animals after estradiol treatment. Estradiol is a potent endogenous antioxidant which suppresses hepatic fibrosis in animal models,and attenuates induction of redox sensitive transcription factors,hepatocyte apoptosis and HSC activation by inhibiting a generation of reactive oxygen species in primary cultures. Variant estrogen receptors are expressed to a greater extent in male patients with chronic liver disease than in females. These lines of evidence suggest that the greater progression of hepatic fibrosis and HCC in men and postmenopausal women may be due,at least in part,to lower production of estradiol and a reduced response to the action of estradiol. A better understanding of the basic mechanisms underlying the sex-associated differences in hepatic fibrogenesis and carciogenesis may open up new avenues for the prevention and treatment of chronic liver disease. | Ichiro Shimizu Nao Kohno Katsuyoshi Tamaki Masayuki Shono Hui-Wei Huang Jiang-Hong He Deng-Fu Yao | 2007 | World Journal of Gastroenterology2007，13，32： | 49 |
| 4 | 橡胶隔振器有限元建模技术及静态弹性特性分析显示文摘以液阻型橡胶隔振器的橡胶主簧为研究对象 ,利用有限元分析软件ABAQUS和ADINA ,建立了轴对称、1/ 4对称和完整的三维有限元模型 ;进行了橡胶隔振器有限元建模技术的数值试验分析 ;探讨了橡胶材料体积近似不可压缩特性的数值确定方法、杂交单元计算稳定性的数值判断方法、单元类型的选择 (包括位移与压力节点的匹配方式及其插值函数阶次、单元积分形式等 )、橡胶材料的超弹性本构关系的确定等 ;阐述了基于有限元方法的橡胶隔振器大变形静态弹性特性有限元分析方法。所预测的橡胶主簧的垂向静态弹性特性与实测结果吻合较好。 | 王利荣 吕振华 Hagiwara Ichiro | 2002 | 汽车工程2002，24，6： | 38 |
| 5 | 中国流行性乙型脑炎病毒分子生物学特性研究显示文摘为了从分子水平了解中国流行性乙型脑炎(乙脑)病毒与国外分离株的差异,对1949年以来在中国乙脑主要流行地区分离的19株乙脑病毒的PrM C区及E蛋白基因区的核苷酸序列进行了对比研究,以期了解不同时间不同地区在中国流行的乙脑病毒的分子差异。结果显示:病毒生物学初步鉴定显示,病毒感染BHK细胞后56h所有细胞均发生病变,约50%以上细胞脱落(CPE );3日龄乳鼠接种病毒72h之内死亡;所有病毒均与乙脑病毒(A2株)抗血清发生阳性反应,但反应强度不同,提示所有病毒均为乙脑病毒。病毒PrM C(456~695)区核苷酸序列与各个国家及地区、各个基因型以及各个年代的73株乙脑病毒相应序列,用Woan RuChen建立的方法进行基因分型分析,结果显示,所有19株中国分离的病毒均属于基因型Ⅲ型,与国外相关文献所报道的乙型脑炎病毒中国分离株的基因型相符。以乙型脑炎病毒疫苗株P3株为标准,对各病毒E蛋白500个氨基酸序列进行分析。各毒株核苷酸差异在0和4 2%之间,氨基酸差异在0和4 0%之间。所有核苷酸差异均为碱基的替换,无论核苷酸或氨基酸均无插入或丢失。大多数核苷酸变异在氨基酸编码的第三位,属于沉默突变,不引起氨基酸变化。18株病毒E蛋白活性结构域与疫苗株P3株相比共有247个氨基酸的差异,平均每株病毒有12 35个氨基酸的差异,? | 李晓宇 宋宏 付士红 王环宇 俞永新 董关木 陶三菊 陈端 Ichiro Kurane 梁国栋 | 2004 | 病毒学报2004，20，3： | 31 |
| 6 | Analysis of aerodynamic and thermodynamic parameters on the grassy marshland surface of Tibetan Plateau | TSUKAMOTO Osamu ISHIKAWA Hirohiko TAMAGAWA Ichiro | 2002 | Progress in Natural Science:Materials International2002，12，1： | 34 |
| 7 | Pancreatic carcinoma coexisting with chronic pancreatitis versus tumor-forming pancreatitis:Diagnostic utility of the time-signal intensity curve from dynamic contrast-enhanced MR imaging显示文摘AIM: To evaluate the ability of the time-signal intensity curve (TIC) of the pancreas obtained from dynamic contrast-enhanced magnetic resonance imaging (MRI) for differentiation of focal pancreatic masses, especially pancreatic carcinoma coexisting with chronic pancreatitis and tumor-forming pancreatitis. METHODS: Forty-eight consecutive patients who underwent surgery for a focal pancreatic mass, including pancreatic ductal carcinoma (n = 33), tumor-forming pancreatitis (n = 8), and islet cell tumor (n = 7), were reviewed. Five pancreatic carcinomas coexisted with longstanding chronic pancreatitis. The pancreatic TICs were obtained from the pancreatic mass and the pancreatic parenchyma both proximal and distal to the mass lesion in each patient, prior to surgery, and were classified into 4 types according to the time to a peak: 25 s and 1, 2, and 3 min after the bolus injection of contrast material, namely, type-Ⅰ, Ⅱ, Ⅲ, and Ⅳ, respectively, and were then compared to the corresponding histological pancreatic conditions. RESULTS: Pancreatic carcinomas demonstrated type-Ⅲ (n = 13) or Ⅳ (n = 20) TIC. Tumor-forming pancreatitis showed type-Ⅱ (n = 5) or Ⅲ (n = 3) TIC. All islet cell tumors revealed type-Ⅰ. The type-Ⅳ TIC was only recognized in pancreatic carcinoma, and the TIC of carcinoma always depicted the slowest rise to a peak among the 3 pancreatic TICs measured in each patient, even in patients with chronic pancreatitis.CONCLUSION: Pancreatic TIC from dynamic MRI provides reliable information for distinguishing pancreatic carcinoma from other pancreatic masses, and may enable us to avoid unnecessary pancreatic surgery and delays in making a correct diagnosis of pancreatic carcinoma, especially, in patients with longstanding chronic pancreatitis. | Yoshitsugu Tajima Tamotsu Kuroki Ryuji Tsutsumi Ichiro Isomoto Masataka Uetani Takashi Kanematsu | 2007 | World Journal of Gastroenterology2007，13，6： | 28 |
| 8 | Spleen-preserving distal pancreatectomy with conservation of the splenic artery and vein显示文摘Preservation of the spleen at distal pancreatectomy has recently attracted considerable attention.Since our first successful trial,spleen-preserving distal pancreatectomy with conservation of the splenic artery and vein for tumors of the pancreas and chronic pancreatitis has been performed more frequently.The technique for spleen-preserving distal pancreatectomy with conservation of the splenic artery and vein are outlined.The splenic vein is identified behind the pancreas and within the thin connective tissue membrane.The connective tissue membrane is cut longitudinally above the splenic vein.An important issue is to remove the splenic vein from the body of the pancreas toward the spleen,since a different approach may be very difficult.The pancreas is preferably removed from the splenic artery toward the head of the pancreas itself.This procedure is much easier than removing the pancreas from the vein side.One patient had undergone distal gastrectomy for duodenal ulcer,with reconstruction by Billroth Ⅱ tehcnique.If distal pancreatectomy with splenectomy had been performed for the lesion of the distal pancreas at the time,the residual stomach would also have to be resected.The potential damage done to the patient by reconstruction of the gastrointestinal tract in combination with distal pancreatectomy and splenectomy would have been much greater than with distal pancreatectomy only with preservation of the spleen and residual stomach.Benign lesions as well as low-grade malignancy of the body and tail of the pancreas may be a possible indication for this procedure. | Wataru Kimura Toshiyuki Moriya Jinfeng Ma Yukinori Kamio Toshihiro Watanabe Mitsukiro Yano Hiroto Fujimoto Koji Tezuka Ichiro Hirai Akira Fuse | 2007 | World Journal of Gastroenterology2007，13，10： | 24 |
| 9 | 为早食道、胃的癌症的诊断的内视镜的超声使用超声探针显示文摘 Endoscopic ultrasound(EUS) devices were first designed and manufactured more than 30 years ago,and since then investigators have reported EUS is effective for determining both the staging and the depth of invasion of esophageal and gastric cancers.We review the present status,the methods,and the findings of EUS when used to diagnose and stage early esophageal and gastric cancer.EUS using high-frequency ultrasound probes is more accurate than conventional EUS for the evaluation of the depth of invasion of superficial esophageal carcinoma.The rates of accurate evaluation of the depth of invasion by EUS using high-frequency ultrasound probes were 70%-88% for intramucosal cancer,and 83%-94% for submucosal invasive cancer.But the sensitivity of EUS using high-frequency ultrasound probes for the diagnosis of submucosal invasive cancer was relatively low,making it difficult to confirm minute submucosal invasion.The accuracy of EUS using highfrequency ultrasound probes for early gastric tumor classification can be up to 80% compared with 63% for conventional EUS,although the accuracy of EUS using high-frequency ultrasound probes relatively decreases for those patients with depressed-type lesions,undifferentiated cancer,concomitant ulceration,expanded indications,type 0-Ⅰ lesions,and lesions located in the upper-third of the stomach.A 92% overall accuracy rate was achieved when both the endoscopic appearance and the findings from EUS using high-frequency ultrasound probes were considered together for tumor classification.Although EUS using high-frequency ultrasound probes has limitations,it has a high depth of invasion accuracy and is a useful procedure to distinguish lesions in the esophagus and stomach that are indicated for endoscopic resection. | Shigetaka Yoshinaga Ichiro Oda Satoru Nonaka Ryoji Kushima Yutaka Saito | 2012 | World Journal of Gastrointestinal Endoscopy2012，4，6： | 21 |
| 10 | Machine learning modeling of superconducting critical temperature显示文摘Superconductivity has been the focus of enormous research effort since its discovery more than a century ago.Yet,some features of this unique phenomenon remain poorly understood;prime among these is the connection between superconductivity and chemical/structural properties of materials.To bridge the gap,several machine learning schemes are developed herein to model the critical temperatures(T_(c))of the 12,000+known superconductors available via the SuperCon database.Materials are first divided into two classes based on their T_(c) values,above and below 10 K,and a classification model predicting this label is trained.The model uses coarse-grained features based only on the chemical compositions.It shows strong predictive power,with out-of-sample accuracy of about 92%.Separate regression models are developed to predict the values of T_(c) for cuprate,iron-based,and low-T_(c) compounds.These models also demonstrate good performance,with learned predictors offering potential insights into the mechanisms behind superconductivity in different families of materials.To improve the accuracy and interpretability of these models,new features are incorporated using materials data from the AFLOW Online Repositories.Finally,the classification and regression models are combined into a single-integrated pipeline and employed to search the entire Inorganic Crystallographic Structure Database(ICSD)for potential new superconductors.We identify>30 non-cuprate and non-iron-based oxides as candidate materials. | Valentin Stanev Corey Oses A.Gilad Kusne Efrain Rodriguez Johnpierre Paglione Stefano Curtarolo Ichiro Takeuchi | 2018 | npj Computational Materials2018，，1： | 21 |
| 11 | Management and associated factors of delayed perforation after gastric endoscopic submucosal dissection显示文摘AIM: To identify the actual clinical management and associated factors of delayed perforation after gastric endoscopic submucosal dissection(ESD).METHODS: A total of 4943 early gastric cancer(EGC) patients underwent ESD at our hospital between January 1999 and June 2012. We retrospectively assessed the actual management of delayed perforation. In addition, to determine the factors associated with delayed perforation, after excluding 123 EGC patients with perforations that occurred during the ESD procedure, we analyzed the following clinicopathological factors among the remaining 4820 EGC patients by comparing the ESD cases with delayed perforation and the ESD cases without perforation: age, sex, chronological periods, clinical indications for ESD, status of the stomach, location, gastric circumference, tumor size, invasion depth, presence/absence of ulceration, histological type, type of resection, and procedure time.RESULTS: Delayed perforation occurred in 7(0.1%) cases. The median time until the occurrence of delayed perforation was 11 h(range, 6-172 h). Three(43%) of the 7 cases required emergency surgery, while four were conservatively managed without surgical intervention. Among the 4 cases with conservative management, 2 were successfully managed endoscopically using the endoloop-endoclip technique. The median hospital stay was 18 d(range, 15-45 d). There were no delayed perforation-related deaths. Based on a multivariate analysis, gastric tube cases(OR = 11.0; 95%CI: 1.7-73.3; P = 0.013) were significantly associated with delayed perforation.CONCLUSION: Endoscopists must be aware of not only the identified factors associated with delayed perforation, but also how to treat this complicationeffectively and promptly. | Haruhisa Suzuki Ichiro Oda Masau Sekiguchi Seiichiro Abe Satoru Nonaka Shigetaka Yoshinaga Takeshi Nakajima Yutaka Saito | 2015 | World Journal of Gastroenterology2015，21，44： | 21 |
| 12 | Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis显示文摘AIM: To evaluate the effect of pyrrolidine dithio- carbamate (PDTC; an NF-κB inhibitor) administered at low (50 mg/kg) and high (100 mg/kg) doses in suppressing colitis in mice with dextran sodium sulfate (DSS)-induced colitis. METHODS: Mice were divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+PDTC-treated groupⅠ(low-dose group), and DSS+PDTC-treated groupⅡ (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue were measured. RESULTS: The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels were significantly lower in DSS+PDTC- treated groupⅡ. CONCLUSION: These findings suggest that PDTC is useful for the treatment of ulcerative colitis. | Ichiro Hirata Shingo Yasumoto Ken Toshina Takuya Inoue Takashi Nishikawa Naoko Murano Mitsuyuki Murano Fang-Yu Wang Ken-ichi Katsu | 2007 | World Journal of Gastroenterology2007，13，11： | 20 |
| 13 | Diagnostic and therapeutic single-operator cholangiopancreatoscopy in biliopancreatic diseases:Prospective multicenter study in Japan显示文摘AIM: To assess the utility and safety of single-operator cholangiopancreatoscopy(SOCPS) using the Spy Glass system in widespread clinical application for biliary and pancreatic diseases.METHODS: This study was a prospective case series conducted in 20 referral centers in Japan. There were 148 patients who underwent SOCPS; 124 for biliary diseases and 24 for pancreatic diseases. The attempted interventions were SOCPS examination, SOCPS-directed tissue sampling, and therapy for stone removal, among others. The main outcomes were related to the procedure success rate in terms of visualizing the target lesions, SOCPS-directed adequate tissue sampling, and complete stone removal. RESULTS: A total of 148 patients were enrolled for the diagnosis of indeterminate biliary and pancreatic lesions or treatment of biliary and pancreatic disease. The overall procedure success rate of visualizing the target lesions was 91.2%(135/148). The overall procedural success rates of visualizing the target lesions of diagnostic SOCPS in the bile duct and pancreatic duct were 95.5%(84/89) and 88.2%(15/17), respectively. Diagnosis: the overall adequate tissue for histologic examination was secured in 81.4% of the 86 patients who underwent biopsy under SOCPS(bile duct, 60/75, 80.0%; pancreatic duct, 10/11, 90.9%). The accuracy of histologic diagnosis using SOCPS-directed biopsies in indeterminate bile duct lesions was 70.7%(53/75). In the pancreatic duct, the accuracy of SOCPS visual impression of intraductal papillary mucinous neoplasm was 87.5%(14/16). Stone therapy: complete biliary and pancreatic stone clearance combined with SOCPS-directed stone therapy using electrohydraulic lithotripsy or laser lithotripsy was achieved in 74.2%(23/31) and 42.9%(3/7) of the patients, respectively. Others: SOCPS using the Spy Glass system was used in cannulation of the cystic duct in two patients and for passing across the obstructed self-expandable metallic stent for a malignant biliary stricture in two patients. All procedures were successful in both SOCPS-guided therapies. The incidence of procedure-related adverse events was 5.4%(8/148). CONCLUSION: SOCPS with direct visualization and biopsy for diagnosis and SOCPS-directed therapy for biliary and pancreatic diseases can be safely performed with a high success rate. | Toshio Kurihara Ichiro Yasuda Hiroyuki Isayama Toshio Tsuyuguchi Taketo Yamaguchi Ken Kawabe Yoshinobu Okabe Keiji Hanada Tsuyoshi Hayashi Takao Ohtsuka Syuhei Oana Hiroshi Kawakami Yoshinori Igarashi Kazuya Matsumoto Kiichi Tamada Shomei Ryozawa Hiroki Kawashima Yutaka Okamoto Iruru Maetani Hiroyuki Inoue Takao Itoi | 2016 | World Journal of Gastroenterology2016，22，5： | 20 |
| 14 | Useful detection of CD147 (EMMPRIN) for pathological diagnosis of early hepatocellular carcinoma in needle biopsy samples显示文摘AIM:To make clear whether CD147 (EMMPRIN) expression in pathological tumor samples with a fine-needle aspiration biopsy is useful for pathological diagnosis of early hepatocellular carcinoma (HCC). METHODS:Twenty-two patients (15 men and 7 women; median age 68 years,range 56-81 years) underwent a liver tissue biopsy in order to make a diagnosis of HCC. Paraffin-embedded liver biopsy tissue samples from 22 patients were stained with anti-CD147 antibody,murine monoclonal antibody 12C3 (MAb12C3) for immunohistochemical analysis. An immunohistochemical analysis of CD147 was performed and the degree of staining compared between tumor and non-tumor tissue. In addition,the degree of staining within tumor tissue was compared according to a number of clinicopathological variables. RESULTS:The degree of staining of CD147 was significantly higher in tumor tissues than non-tumor tissues,even in tumors less than 15 mm in diameter.The expression of this protein was significantly elevated in HCC tissue specimens from patients with a low value of serum AST and γ-GTP. | Satoshi Mamori Keisuke Nagatsuma Tomokazu Matsuura Kiyoshi Ohkawa Hiroshi Hano Masaharu Fukunaga Masato Matsushima Yoshifumi Masui Nao Fushiya Hiroshi Onoda Yasuyuki Searashi Ichiro Takagi Hisao Tajiri | 2007 | World Journal of Gastroenterology2007，13，21： | 20 |
| 15 | Using sorbents to control heavy metals and particulate matter emission during solid fuel combustion显示文摘Some of the heavy metals in coal and wastes vaporize during combustion, concentrate in fine particulates, and emit with the flue gas into the atmosphere, to produce adverse effect on environment and health. This study first investigates the fate of the heavy metal species, especially Pb, Cd and Cr, known as semi-volatile, in various flue gases, especially in the presence of HCl and SO2, by chemical equilibrium calculation, in which, Si and Ca were proposed as base sorbent materials to capture Pb, Cd and Cr. Then Si-and Ca-based compounds as well as waste materials used as sorbents were optimized to capture the heavy metals. Finally, the optimal sorbent was tested in actual burning of dried sewage sludge as solid fuel, to evaluate the effectiveness of the sorbent. Calculated results show that Cl increases the volatility of most heavy metals, while SO2 enhances formation of condensed phases. Among the sorbents tested, kaolin appears most efficient to capture Pb and Cd. For sludge combustion with kaolin addition, both Pb and Cd were shifted from sub-micron to macro-sized particles, and accompanied by considerable decrease of 0.1 m particles. | Hong Yao Ichiro Naruse | 2009 | Particuology2009，7，6： | 18 |
| 16 | Minimally invasive surgery for upper gastrointestinal cancer: Our experience and review of the literature显示文摘Minimally invasive surgery(MIS) for upper gastro-intestinal(GI) cancer, characterized by minimal access, has been increasingly performed worldwide. It not only results in better cosmetic outcomes, but also reduces intraoperative blood loss and postoperative pain, leading to faster recovery; however, endoscopically enhanced anatomy and improved hemostasis via positive intracorporeal pressure generated by CO2 insufflation have not contributed to reduction in early postoperative complications or improvement in long-term outcomes. Since 1995, we have been actively using MIS for operable patients with resectable upper GI cancer and have developed stable and robust methodology in conducting totally laparoscopic gastrectomy for advanced gastric cancer and prone thoracoscopic esophagectomy for esophageal cancer using novel technology including da Vinci Surgical System(DVSS). We have recently demonstrated that use of DVSS might reduce postoperative local complications including pancreatic fistula after gastrectomy and recurrent laryngeal nerve palsy after esophagectomy. In this article, we present the current status and future perspectives on MIS for gastric and esophageal cancer based on our experience and a review of the literature. | Koichi Suda Masaya Nakauchi Kazuki Inaba Yoshinori Ishida Ichiro Uyama | 2016 | World Journal of Gastroenterology2016，22，19： | 13 |
| 17 | Protective effect of estradiol on hepatocytic oxidative damage显示文摘AIM: To examine the protective effect of estradiol on the cultured hepatocytes under oxidative stress.METHODS: Hepatocytes of rat were isolated by using perfusion method, and oxidative stress wes induced by a serum-free medium and FeNTA. MDA level was determined with TBA method. Cell damage was assessed by LDH assay. Apoptosis of hepatocytes was assessed with cytoflowmetric analysis. Expression of Bcl-xl in cultured hepatocytes was detected by Western blot.The radicalscavenging activity of estradiol was valued by its ability to scavenge the stable free radical of DDPH.RESULTS: Oxidative stress increased LDH (from 168 ± 25 x10-6 IU. cell 1 to 780 ± 62 x 10-6 IU. cell-1 ) and MDA(from 0.28 ±0.07 x 10-6 nmol. cell-1 to 1. 35 ± 0.12 × 10-6 nmol. cell-1 ) levelsin cultured hepatocyte, and estradiol inhibited both LDH andMDA production in a dose dependent manner. In thepresence of estradiol t0-6 mol. L-1, 107 mol. L-1 and 10-8 mol.L-1 ,the LDH levels are 410 ± 53 × 10-6 IU. cell-1 ( P < 0.01 vsoxidative group), 530 ± 37 × 10-6 IU. cell-1 ( P < 0. 01 vsoxidative group), 687 ± 42 x 10-6 IU. cell-1 ( P < 0.05 vsoxidative group) respectively, and the MDA level are 0.71 ±0.12 x 10-6 nmol. cell-1 ( P < 0.01 vs oxidative group), 0.97 ± 0.11 × 10-6 nmol. cell-1 ( P < 0.01 vs oxidative group) and 1.27 ±0. 19 x 10-6 nmol. cell-1 respectively. Estradiol suppressedapoptosis of hepatocytes induced by oxidative stress,administration of estradiol (10-6 mol/ L)decreased theapoptotic rate of hepatocytes under oxidative stress from 18.6 ± 1.2% to 6.5 ± 2.5%, P < 0.01. Bcl-xl expression wasrelated to the degree of liver cell damage due to oxidativestress, and estradiol showed a protective action.CONCLUSION: Estmdiol protects hepatocytes from oxidativedamage by means of its antioxidant activity. | Ichiro Shimizu Toshihiro Omoya Susumu Ito | 2002 | World Journal of Gastroenterology2002，8，2： | 13 |
| 18 | Biomimetic Drag Reduction Study on Herringbone Riblets of Bird Feather显示文摘 | Huawei Chen Fugang Rao Xiaopeng Shang Deyuan Zhang Ichiro Hagiwara | 2013 | Journal of Bionic Engineering2013，10，3： | 12 |
| 19 | 新疆出血热病毒分子流行病学研究显示文摘目的研究新疆出血热病毒(XHF)分子流行病学,揭示其与相关病毒之间关系,分析XHF的流行来源和分布特点。方法RTPCR检测20012002年XHF患者和蜱标本中XHF病毒S基因,阳性标本直接进行核苷酸序列测定。计算机软件进行S基因部分片段和S全基因序列同源性比较和S基因、M基因的种系发生树分析。结果不同年份人和蜱来源的病毒S基因部分片段核苷酸序列均显示较高同源性(97.3%~100%)。S基因进化树分析将病毒分成了欧洲、非洲和亚洲3组,其中亚洲毒株由中亚分离株和中国分离株组成,中国所有的XHF病毒S基因(同源性93.0%~99.5%)均集中在一个分枝下形成独立的一组,明显区别于世界上其他地区分离株(同源性81.40%~96.4%)。M基因进化树分析表明序列间差异不完全与病毒分离的地理区域相关。结论S基因分析显示XHF病毒蜱分离株与人分离株遗传背景接近,我国XHF病毒有共同的进化途径和基因结构特点,并具明显的地域性。 | 唐青 赵秀芹 王环宇 司马义巴吾东 张玉贞 Masayuki Saijo Shigeru Morikawa 梁国栋 Ichiro Kurane | 2005 | 中华实验和临床病毒学杂志2005，19，4： | 11 |
| 20 | Xanthogranulomatous cholecystitis mimicking gallbladder carcinoma with a false-positive result on fluorodeoxyglucose PET显示文摘Recently, several reports have demonstrated that fluorine-18 fluorodeoxyglucose positron emission tomography (FDG-PET) is useful in differentiating between benign and malignant lesions in the gallbladder. However, there is a limitation in the ability of FDG-PET to differentiate between inflammatory and malignant lesions. We herein present a case of xanthogranulomatous cholecystitis misdiagnosed as gallbladder carcinoma by ultrasonography and computed tomography. FDG-PET also showed increased activity. In this case, FDG-PET findings resulted in a false-positive for the diagnosis of gallbladder carcinoma.Recently, several reports have demonstrated that fluorine-18 fluorodeoxyglucose positron emission tomography (FDG-PET) is useful in differentiating between benign and malignant lesions in the gallbladder. However, there is a limitation in the ability of FDG-PET to differentiate between inflammatory and malignant lesions. We herein present a case of xanthogranulomatous cholecystitis misdiagnosed as gallbladder carcinoma by ultrasonography and computed tomography. FDG-PET also showed increased activity. In this case, FDG-PET findings resulted in a false-positive for the diagnosis of gallbladder carcinoma. | Isamu Makino Takahiro Yamaguchi Nariatsu Sato Toshiaki Yasui Ichiro Kita | 2009 | World Journal of Gastroenterology2009，15，29： | 11 |
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