|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | CircRNA-SORE mediates sorafenib resistance in hepatocellular carcinoma by stabilizing YBX1显示文摘Sorafenib is the first-line chemotherapeutic therapy for advanced hepatocellular carcinoma(HCC).However,sorafenib resistance significantly limits its therapeutic efficacy,and the mechanisms underlying resistance have not been fully clarified.Here we report that a circular RNA,circRNA-SORE(a circular RNA upregulated in sorafenib-resistant HCC cells),plays a significant role in sorafenib resistance in HCC.We found that circRNA-SORE is upregulated in sorafenib-resistant HCC cells and depletion of circRNA-SORE substantially increases the cell-killing ability of sorafenib.Further studies revealed that circRNA-SORE binds the master oncogenic protein YBX1 in the cytoplasm,which prevents YBX1 nuclear interaction with the E3 ubiquitin ligase PRP19 and thus blocks PRP19-mediated YBX1 degradation.Moreover,our in vitro and in vivo results suggest that circRNA-SORE is transported by exosomes to spread sorafenib resistance among HCC cells.Using different HCC mouse models,we demonstrated that silencing circRNA-SORE by injection of siRNA could substantially overcome sorafenib resistance.Our study provides a proof-of-concept demonstration for a potential strategy to overcome sorafenib resistance in HCC patients by targeting circRNA-SORE or YBX1. | Junjie Xu Lin Ji Yuelong Liang Zhe Wan Wei Zheng Xiaomin Song Kirill Gorshkov Qiming Sun Hui Lin Xueyong Zheng Jiang Chen Ren-an Jin Xiao Liang Xiujun Cai | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 2 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 3 | De novo Assembly of Pen Shell(Atrina pectinata) Transcriptome and Screening of Its Genic Microsatellites显示文摘The pen shell(Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific countries. However, limiting genomic and transcriptomic data have hampered its genetic investigations. In this study, the transcriptome of A. pectinata was deeply sequenced using Illumina pair-end sequencing technology. After assembling, a total of 127263 unigenes were obtained. Functional annotation indicated that the highest percentage of unigenes(18.60%) was annotated on GO database, followed by 18.44% on PFAM database and 17.04% on NR database. There were 270 biological pathways matched with those in KEGG database. Furthermore, a total of 23452 potential simple sequence repeats(SSRs) were identified, of them the most abundant type was mono-nucleotide repeats(12902, 55.01%), which was followed by di-nucleotide(8132, 34.68%), tri-nucleotide(2010, 8.57%), tetra-nucleotide(401, 1.71%), and penta-nucleotide(7, 0.03%) repeats. Sixty SSRs were selected for validating and developing genic SSR markers, of them 23 showed polymorphism in a cultured population with the average observed and expected heterozygosities of 0.412 and 0.579, respectively. In this study, we established the first comprehensive transcript dataset of A. pectinata genes. Our results demonstrated that RNA-Seq is a fast and cost-effective method for genic SSR development in non-model species. | SUN Xiujun LI Dongming LIU Zhihong ZHOU Liqing WU Biao YANG Aiguo | 2017 | Journal of Ocean University of China2017，16，5： | 2 |
| 4 | Printability during projection-based 3D bioprinting显示文摘Since projection-based 3D bioprinting(PBP)could provide high resolution,it is well suited for printing delicate structures for tissue regeneration.However,the low crosslinking density and low photo-crosslinking rate of photocurable bioink make it difficult to print fine structures.Currently,an in-depth understanding of the is lacking.Here,a research framework is established for the analysis of printability during PBP.The gelatin methacryloyl(GelMA)-based bioink is used as an example,and the printability is systematically investigated.We analyze the photo-crosslinking reactions during the PBP process and summarize the specific requirements of bioinks for PBP.Two standard quantized models are established to evaluate 2D and 3D printing errors.Finally,the better strategies for bioprinting five typical structures,including solid organs,vascular structures,nerve conduits,thin-wall scaffolds,and micro needles,are presented. | Kang Yu Xinjie Zhang Yuan Sun Qing Gao Jianzhong Fu Xiujun Cai Yong He | 2022 | Bioactive Materials2022，7，5： | 2 |
| 5 | Isolation and Characterization of Melanin Pigment from Yesso Scallop Patinopecten yessoensis显示文摘Melanin is one of the essential compounds in the pigments of molluscan shells.However,the effects of melanin on color variations in molluscs are largely unknown.Our previous study suggests that Yesso scallop Patinopecten yessoensis might contain melanin pigment in the dark brown shell.We therefore isolated melanin from the pigmented shells using hydrochloric acid method,and characterized the types of melanin pigments by spectrophotometry.The purified melanin,which was verified by spectrophotometry scanning and HPLC analysis,showed the typical characteristics of melanin absorption spectra and HPLC chromatograms.The contents of pheomelanin and eumelanin in pigmented shells,which were determined by the linear standard curve of melanin at 405 nm and 350 nm absorbance,were 48.23 ? 1.350 and 157.65 ? 5.905 mg,respectively.The present results indicate that the brown-pigmented shells of scallops comprise approximately 76.6% of eumelanin and 23.4% of pheomelanin,which supports the presence of eumelanin-rich pigment in scallop shells.Therefore,the combination of hydrochloric acid extraction and spectrophotometric quantification is a rapid and efficient method to isolate and quantify melanin in shells.This will facilitate the melanin studies related to shell color polymorphism and the selective breeding of bivalves with different shell colors. | SUN Xiujun WU Biao ZHOU Liqing LIU Zhihong DONG Yinghui YANG Aiguo | 2017 | Journal of Ocean University of China2017，16，2： | 2 |
| 6 | Analysis and Research on friction-free cylinder of modal testing suspension system 显示文摘 | Jianhui Sun Xiaohang Shan Xiujun Zhang Li Zhang etc | 2008 | Robotics Control and Manufacturing Technology2008，4，： | 1 |
| 7 | Developmental Dynamics of the Larval Muscle System of Bay Scallop(Argopecten irradians)显示文摘Though the larval development of bivalves has been extensively studied for commercial purposes,the dynamic development of larval muscle system remains largely unknown.In this study,we characterized the larval muscle system at different developmental stages(D-shaped veligers,umbo veligers and spats)in the bay scallop(Argopecten irradians)by phalloidin staining and under a confocal microscopy.The functional muscles are initially established at the early stage of veligers,which have four pairs of velar retractors and one anterior adductor.At the veliger stage,the velum and posterior retractor muscles are functionally important for velar contractility but undergo an irreversible shrink until they disappear at the end of the larval stage.During metamorphosis,three crucial modifications take place in the larval muscle system.The metamorphosis process involves the gradual degeneration of velum retractors,mantle margin development from an unfolded to a three-fold state,and remodeling of the adductor muscle system from dimyarian(two adductors)to monomyarian condition(one adductor)as in juveniles/adults.All retractor muscles are composed of striated muscle,but both anterior and posterior adductors have smooth and striated components.These findings highlight that the morphological changes at different stages are typical features of myogenesis in scallops.The present knowledge on the developmental dynamics of myogenesis in the bay scallop will not only improve our understanding of phenotypic diversity of larval myoanatomy in bivalves,but also provide useful information on the larval culture in hatcheries. | SUN Xiujun LIU Zhihong ZHOU Liqing WU Biao YANG Aiguo TIAN Jiteng | 2020 | Journal of Ocean University of China2020，19，2： | 1 |
| 8 | Distributed Power Allocation for Coordinated Multipoint Transmissions in Distributed Antenna Systems 显示文摘 | ZHANG Xiujun SUN in CHEN Xiang ZHOU Shidong WANG Jing SHROFF B | 2013 | IEEE Transactions on Wireless Communica- tions2013，12，5： | 1 |
| 9 | Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Manila Clam Ruditapes philippinarum Under Hypoxic Stress显示文摘Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate results.To find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia,different tissues were used and compared to evaluate the stability of candidate reference genes under low oxygen stress(DO 0.5mgL^(−1) and DO 2.0mgL^(−1))and normal condition(DO 7.5mgL^(−1)).Seven candidate reference genes were selected to evaluate the stability of their expression levels.The reference genes were evaluated by Delta Ct,BestKeeper,NormFinder and geNorm,and then screened by RefFinder calculation.Under hypoxic stress of 0.5mgL^(−1),the most suitable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were TUB and HIS,respectively.For hypoxic stress of 2.0mgL^(−1),the most stable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were RPS23 and EF1A,respectively.At the normal condition,HIS and EF1A were identified as the optimal internal reference genes in gill and hepatopancreas respectively,and GFRP2 was the best internal reference gene for axe foot and adductor muscle.The present findings will provide important basis for the selection of reference genes for qRT-PCR analysis of gene expression level in bivalves under hypoxic stress,which might be helpful for the analysis of other molluscs too. | JING Hao ZHOU Liqing GONG Miao TU Kang LIU Zhihong WU Biao SUN Xiujun | 2023 | Journal of Ocean University of China2023，22，4： | 0 |
| 10 | Genome assembly of the snow lotus species Saussurea involucrata provides insights into acacetin and rutin biosynthesis and tolerance to an alpine environment显示文摘Dear Editor,Understanding the evolution and survival mechanisms of endangered wild medicinal herbs is crucial for their cultivation,utilization,and conservation.The snow lotus species Saussurea involucrata(Kar.&Kir.)Sch.Bip.(2n=32)(i.e.the well-known Tianshan snow lotus)which belongs to the eudicot family Asteraceae,is a famous traditional Chinese medicinal herb having anti-inflammatory. | Yanxia Sun Aidi Zhang Jacob BLandis Wei Shi Xiujun Zhang Hang Sun Hengchang Wang | 2023 | Horticulture Research2023，10，10： | 0 |
| 11 | Abnormal Karyotype of Pen Shell (Atrina pectinata) During Its Early Embryonic Development in Late Breeding Season显示文摘The pen shell,Atrina pectinata,distributes globally.It is one of the most important edible bivalves in east Asian countries.However,there are multiple difficulties in rearing pen shell larvae and juveniles because of their high mortality.To understand the mechanism underlining such high mortality at the early embryonic development stage,we obtained approximately 100 million larvae during later breeding season in June,2017,and checked a large amount of mitotic chromosomal plates of the early embryos and post-spawning gonads tissue slices of their parents.The results showed that most diploid A.pectinata embryos have 17 pairs of chromosomes(2n=34)as their parents do.The first pair of particularly large chromosomes are heterotypic in some diploid embryos while they are homomorphic in others.The primary sex-determination chromosome type is XX/XY.A lot of triploid,pentaploid and aneuploid embryos with different numbers of the largest homomorphic or heteromorphic chromosomes were found due to the degeneration of overmatured parent gonads which hold normal karyotype.These larvae will die even though most of them may develop into the trochophore stage with 34 chromosomes.Genetic deficiency of chromosomes will cause a high rate of mortality in early embryos in late breeding season.These findings should enrich the current knowledge of juvenile pen shell aquaculture. | ZHOU Liqing WANG Xuemei YANG Aiguo WU Biao SUN Xiujun LIU Zhihong CHEN Siqing ZHAO Dan | 2020 | Journal of Ocean University of China2020，19，4： | 0 |
| 12 | An experimental research into endostatin microbubble combined with focused ultrasound for anti-tumor angiogenesis in colon cancer显示文摘Objective:to evaluate the therapeutic effect of targeted endostatin-loaded microbubbles,combined with improved,focused,directional ultrasound radiation for inhibition of subcutaneous translocation in situ colon tumor angiogenesis in colon cancer.Methods:65 BALB/c nude mice with subcutaneous translocation in situ colon tumors were randomly divided into five groups.Group A was the control group,without any treatments.In Group B,the mouse was treated with ultrasonic radiation.In Group C,the mouse was treated with ultrasonic radiation combined with empty SonoVue microbubbles.In Group D,the mouse was treated with ultrasonic radiation combined with empty Targestar-SA microbubbles.In Group E,the mouse was treated with ultrasonic radiation combined with endostatin microbubbles.The tumor size was measured before and 1,14,and 28 days after irradiation.The peak intensity(PI),regional blood volume(RBV)and regional blood flow(RBF)were recorded using contrast-enhanced ultrasound.The tumor tissue was removed for pathological examination;the tumor necrosis area and microvascular density(MVD)were evaluated by immunohistochemistry.Results:Tumors in Groups C,D and E were significantly smaller than in Groups A and B at 28 days after irradiation,with Group E the smallest.PI,RBF and RBV of Groups C,D,and E were significantly decreased 28 days after radiation with Group E the lowest,and significantly lower than Groups A and B(all P<0.05).The tumor tissue necrosis area of Group E was clearly greater while MVD was obviously lower than the other groups(all P<0.01)at 28 days after treatment.Conclusion:The targeted endostatin microbubbles,combined with focused,directional ultrasound radiation can damage tumor microvasculature of subcutaneous colon translocation in situ colon cancer,as well as inhibit the tumor angiogenesis. | Xiufeng Zhang Guangen Yang Ying Zhang Pintong Huang Jianming Qiu Yu Sun Zhong Shen Xiujun Liao Hongsheng Xia Shuxian Shao Dong Wang | 2014 | Gastroenterology Report2014，2，1： | 0 |
| 13 | Adaptive Tensor Rank Approximation for Multi-View Subspace Clustering显示文摘Multi-view subspace clustering under a tensor framework remains a challenging problem,which can be potentially applied to image classification,impainting,denoising,etc.There are some existing tensor-based multi-view subspace clustering models mainly making use of the consistency in different views through tensor nuclear norm(TNN).The diversity which means the intrinsic difference in individual view is always ignored.In this paper,a new tensorial multi-view subspace clustering model is proposed,which jointly exploits both the consistency and diversity in each view.The view representation is decomposed into view-consistent part(low-rank part)and view-specific part(diverse part).A tensor adaptive logdeterminant regularization(TALR)is imposed on the low-rank part to better relax the tensor multi-rank,and a view-specific sparsity regularization is applied on the diverse part to ensure connectedness property.Although the TALR minimization is not convex,it has a closedform analytical solution and its convergency is validated mathematically.Extensive evaluations on six widely used clustering datasets are executed and our model is demonstrated to have the superior performance. | SUN Xiaoli HAI Yang ZHANG Xiujun XU Chen | 2023 | Chinese Journal of Electronics2023，32，4： | 0 |
| 14 | CircRNA-SORE mediates sorafenib resistance inhepatocellular carcinoma by stabilizing YBX1显示文摘Sorafenib is the first-line chemotherapeutic therapy for advanced hepatocellular carcinoma(HCC).However,sorafenib resistance significantly limits its therapeutic efficacy,and the mechanisms underlying resistance have not been fully clarified.Here we report that a circular RNA,circRNA-SORE(a circular RNA upregulated in sorafenib-resistant HCC cells),plays a significant role in sorafenib resistance in HCC.We found that circRNA-SORE is upregulated in sorafenib-resistant HCC cells and depletion of circRNA-SORE substantially increases the cell-killing ability of sorafenib.Further studies revealed that circRNA-SORE binds the master oncogenic protein YBX1 in the cytoplasm,which prevents YBX1 nuclear interaction with the E3 ubiquitin ligase PRP19 and thus blocks PRP19-mediated YBX1 degradation.Moreover,our in vitro and in vivo results suggest that circRNA-SORE is transported by exosomes to spread sorafenib resistance among HCC cells.Using different HCC mouse models,we demonstrated that silencing circRNA-SORE by injection of siRNA could substantially overcome sorafenib resistance.Our study provides a proof-of-concept demonstration for a potential strategy to overcome sorafenib resistance in HCC patients by targeting circRNA-SORE or YBX1. | Junjie Xu Lin Ji Yuelong Liang Zhe Wan Wei Zheng Xiaomin Song Kirill Gorshkov Qiming Sun Hui Lin Xueyong Zheng Jiang Chen Ren-an Jin Xiao Liang Xiujun Cai | 2021 | Signal Transduction and Targeted Therapy2021，6，1： | 0 |
| 15 | Validation of NCEP and OAFlux air-sea heat fluxes using observations from a Black Pearl wave glider显示文摘Latent and sensible heat fluxes based on observations from a Black Pearl wave glider were estimated along the main stream of the Kuroshio Current from the East China Sea to the east coast of Japan,from December 2018 to January 2019.It is found that the data obtained by the wave glider were comparable to the sea surface temperature data from the Operational Sea Surface Temperature and Sea Ice Analysis and the wind field data from WindSat.The Coupled Ocean Atmosphere Response Experiment 3.0(COARE 3.0)algorithm was used to calculate the change in air-sea turbulent heat flux along the Kuroshio.The averaged latent heat flux(LHF)and sensible heat flux(SHF)were 235 W/m^(2)and 134 W/m^(2),respectively,and the values in the Kuroshio were significant larger than those in the East China Sea.The LHF and SHF obtained from Objectively Analyzed Air-Sea Fluxes for the Global Oceans(OAFlux)were closer to those measured by the wave glider than those obtained from National Centers for Environmental Prediction(NCEP)reanalysis products.The maximum deviation occurred in the East China Sea and the recirculation zone of the Kuroshio(deviation of SHF>200 W/m^(2);deviation of LHF>400 W/m^(2)).This indicates that the NCEP and OAFlux products have large biases in areas with complex circulation.The wave glider has great potential to observe air-sea heat fluxes with a complex circulation structure. | Huabin Mao Xiujun Sun Chunhua Qiu Yusen Zhou Hong Liang Hongqiang Sang Ying Zhou Ying Chen | 2021 | Acta Oceanologica Sinica2021，40，10： | 0 |
| 16 | A comparison of multiplatform wind products in the South China Sea during summer and autumn in 2019显示文摘Sea surface wind(SSW)observations from a newly developed“Black Pearl”wave glider,the Chinese-French Oceanography Satellite(CFOSAT),the HY-2A microwave scatterometer,and a recently released high-resolution atmospheric reanalysis(ERA5)are evaluated with respect to in-situ buoy observations(115.46°E,19.85°N)from the South China Sea.Buoy observations from June to November 2019 are used to evaluate the wind estimates from the different platforms.The comparisons show that the HY-2A and CFOSAT scatterometer wind speeds have mean root mean square errors(RMSEs)of approximately 1.6 and 1.6 m/s,respectively,and the corresponding mean wind direction RMSEs are approximately 19°and 17°,which indicates that these satellite retrievals meet the requirements of design engineering missions.The wind speed and wind direction RMSEs of ERA5 are approximately 1.9 m/s and 33°,respectively.The correlation coefficients between the HY-2A,CFOSAT,and ERA5 wind speeds and the buoy observations are 0.86,0.85,and 0.84,respectively,and the corresponding coefficients of the wind direction are 0.98,0.98,and 0.93,respectively,at a 95%confidence level.However,the wind sensor in the wave glider provides relatively poor-quality observations compared with the buoy measurements and has higher wind speed and wind direction RMSEs of 2.9 m/s and 50.1°,respectively.Taylor diagrams are utilized to illustrate comprehensive wind comparisons between the multiplatform observations and buoy observations.The results help identify the basic biases in SSWs among different products and enhance confidence in the future use of SSW data for studies of upper ocean dynamics and climate analysis.Suggestions are also off ered to help improve the design of next-generation wave gliders. | Yuxin LIU Mingsen LIN Xingwei JIANG Xiujun SUN Xiangzhou SONG | 2021 | Journal of Oceanology and Limnology2021，39，6： | 0 |
| 17 | Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China显示文摘Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological signi?cance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We studied the karyotype of B. liberate by light microscopy using air-drying and spreading methods. We obtained mitotic chromosomal plates from B. liberate larvae, and from adult B. liberate females subsequent to egg-laying. The results revealed that B. liberate has 53 pairs of chromosomes(i.e., n =53 and 2 n =106), a characteristic shared with four species of crab. The karyogram of B. liberate consists of 25 metacentric, 14 submetacentric, 11 subtelocentric and 3 telocentric pairs. We did not ?nd any heteromorphosis sex chromosomes. Tissue from larvae, gills and ovaries can be used for chromosomal investigations, and we found similar lampbrush chromosomes in ovary cells. Comparatively speaking, larvae tissue is more practical, and ovary tissue is more suitable for the preparation of lampbrush chromosomes. B. liberate is more closely related to crabs than to shrimps, based on the numbers of chromosomes. The B. liberate karyotype reported here provides a basis for further comparative cytogenetic studies of species populations. | ZHOU Liqing WANG Xuemei WU Biao SUN Xiujun ZHAO Qing ZHANG Gaowei LIU Zhihong YANG Aiguo | 2019 | Journal of Oceanology and Limnology2019，37，1： | 0 |
| 18 | The Effect of Chemical Cues on the Settlement of Sea Cucumber(Apostichopus japonicus) Larvae显示文摘The effects of four ions and eight neuroactive compounds on inducing larval settlement of A. japonicus were assessed in the present study. All bioassays were conducted in 60 × 9 mm Petri dishes, each contained 10 mL of the test solution and 10 doliolaria larvae. There were significant inductive effects of K+(10- mmol L-1), NH+4(0.1 mmol L1), GABA(10-3 mol L-1), acetylcholine(10-5 mol L-1), L-DOPA(10-5 mol L-1), norepinephrine(10-5 mol L-1) and dopamine(10-7 mol L-1 and 10-5 mol L-1) on the settlement of sea cucumber larvae. L-DOPA and dopamine are the most efficient chemical cues to induce A. japonicus larvae to settle. The highest percentage of larval settlement was induced by 10-5 mol L-1 L-DOPA and dopamine(33% and 40%) compared to the control(7%). However, Ca2+, Mg2+, choline, serotonin, and epinephrine were less effective on larval settlement at all tested concentrations. This study evaluated the stability and feasibility of chemical cues for larval settlement in different culture systems, which can be applied to improve the hatchery production of this valuable species. | SUN Xiujun LI Qi YU Hong KONG Lingfeng | 2014 | Journal of Ocean University of China2014，13，2： | 0 |
| 19 | Multiscale design of stiffening and ROS scavenging hydrogels for the augmentation of mandibular bone regeneration显示文摘Although biomimetic hydrogels play an essential role in guiding bone remodeling,reconstructing large bone defects is still a significant challenge since bioinspired gels often lack osteoconductive capacity,robust mechanical properties and suitable antioxidant ability for bone regeneration.To address these challenges,we first engineered molecular design of hydrogels(gelatin/polyethylene glycol diacrylate/2-(dimethylamino)ethyl methacrylate,GPEGD),where their mechanical properties were significantly enhanced via introducing trace amounts of additives(0.5 wt%).The novel hybrid hydrogels show high compressive strength(>700 kPa),stiff modulus(>170 kPa)and strong ROS-scavenging ability.Furthermore,to endow the GPEGD hydrogels excellent osteoinductions,novel biocompatible,antioxidant and BMP-2 loaded polydopamine/heparin nanoparticles(BPDAH)were developed for functionalization of the GPEGD gels(BPDAH-GPEGD).In vitro results indicate that the antioxidant BPDAH-GPEGD is able to deplete elevated ROS levels to protect cells viability against ROS damage.More importantly,the BPDAH-GPEGD hydrogels have good biocompatibility and promote the osteo differentiation of preosteoblasts and bone regenerations.At 4 and 8 weeks after implantation of the hydrogels in a mandibular bone defect,Micro-computed tomography and histology results show greater bone volume and enhancements in the quality and rate of bone regeneration in the BPDAH-GPEGD hydrogels.Thus,the multiscale design of stiffening and ROS scavenging hydrogels could serve as a promising material for bone regeneration applications. | Yanlin Wu Xuan Li Yimin Sun Xiujun Tan Chenglin Wang Zhenming Wang Ling Ye | 2023 | Bioactive Materials2023，，2： | 0 |
| 20 | Analysis of Gas Flow Rate and Pressure Loss of Medical Mask Differential Pressure Tester显示文摘The objective of this study is to find a suitable method to overcome the pressure loss problem in the gas pipe during the gas exchange detection of medical masks.Based on the European Standards EN 14683,the parameters of a medical mask differential pressure tester were selected,subsequently two schemes of gas pipe layouts were designed,including four kinds of pipe diameter which are 4,5,6.5,and 8mm respectively.Lastly,the models of each scheme were established and imported into Fluent,and the relevant parameters were set for simulation.After data analysis,the results showed that among the four different pipe diameters,the pressure loss of 8mm diameter of the pipe was lower in both the schemes,additionally the pressure loss of the second scheme(the gas pipe was short and smooth)was lower under the same pipe diameter.At the flow rate of v=8L/min,the pressure loss from the inlet to the measurement point is less than 200Pa,and the estimated measurement error is less than 1.5%.In conclusion,shortening the length of the pipe,and increasing the diameter of the pipe can reduce the gas pressure loss,subsequently improve the measurement accuracy of the medical mask differential pressure tester. | Zechen Lin Qing Zhang Jianjun Sun Xiujun Yao | 2022 | Journal of Clinical and Nursing Research2022，6，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员