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| 1 | 玉米产量及构成因子主效和上位性QTL的全基因组扫描分析显示文摘在此前构建的含174个分子标记的连锁图基础上,新增31个分子标记,并利用统计分析的方法对基因型数据进行检测,剔除基因型明显有误的数据,构建了共包含205个分子标记,平均间距为11.2 cM,覆盖玉米全基因组10条染色体的分子标记连锁图.基于统计学的基因型数据检测,能显著提高连锁图构建的准确性.结合一年两点6个重复的田间实验,利用软件R/qtl进行主效和上位性QTL分析,同时利用多区间作图法(MIM)验证主效QTL和主效QTL互作的真实性和位置.通过MIM方法,检测到控制产量、行粒数、行数和百粒重的主效QTL分别为5,5,7和5个,分别能解释35.3%,37.4%(含1个互作QTL),61.5%和39.7%的遗传变异;除行粒数的2个QTL之间存在显著性互作之外,其他性状的主效QTL之间都没有检测到显著性互作存在.同时,还检测到24个上位性互作QTL,这些OTL涉及的位点几乎分布于所有10条染色体.这些互作QTL以主效QTL与无显著效应基因座之间的互作居多,对所有4个性状而言比例接近三分之二.这也说明主效QTL除了单独起作用之外还可以通过与其他没有显著效应的基因座之间互作来影响性状表达.结果表明,上位性QTL在玉米产量性状的遗传中可能与主效QTL一样也起着重要的作用. | 严建兵 汤华 黄益勤 郑用琏 Subhash Chander 李建生 | 2006 | 科学通报2006，51，12： | 23 |
| 2 | 高油和普通玉米自交系类胡萝卜素和生育酚含量的比较分析显示文摘建立了利用高效液相色谱法同时测定玉米籽粒类胡萝卜素和生育酚各组分含量的技术体系,分析了112份黄色胚乳玉米自交系的类胡萝卜素和生育酚含量,其中包括32份高油自交系和80份在生产上广泛应用的普通玉米自交系。结果表明,不同自交系间存在广泛的变异,各组分含量变幅最大的是α-生育酚,含量相差达162倍;变幅最小的是δ-生育酚,含量相差也接近4倍。类胡萝卜素各组分含量在高油和普通玉米自交系之间并没有显著差异,但普通玉米的变异范围更为广泛;高油玉米生育酚各组分含量显著高于普通玉米,其中γ-生育酚、α-生育酚和总生育酚含量的均值分别是普通玉米均值的2.4、2.5和2.4倍。尽管供试的高油玉米材料仅32份,但其生育酚的某些组分比普通玉米具有更广泛的遗传变异。这为进一步的玉米高油、高维生素A原、高维生素E,即'三高'品质成分育种提供了有益参考。 | 周毅 付志远 李青 徐淑兔 CHANDER Subhash 杨小红 李建生 严建兵 | 2009 | 作物学报2009，35，11： | 12 |
| 3 | 一种基于聚合酶链式反应检测SNP的方法显示文摘SNP是具有广泛利用潜力的第3代分子标记,本文旨在开发一种利用PCR技术快速检测SNP的方法。设计思路是:根据已知SNP位点设计2条特异正向引物,其最后一个碱基分别与已知SNP的2个碱基相同,同时在1条引物的5′端添加1段20 bp左右的其他物种的特异序列(如细菌DNA序列),然后选择1条合适的反向引物;最后同时加入3条引物,通过梯度PCR选择合适的退火温度进行PCR反应。利用这一方法成功将玉米的ZDS基因定位在玉米第7染色体短臂7.02 Bin。这种检测SNP的方法设计简单,费用低廉,尤其适合SNP标记的分子标记连锁图构建或者基因定位。 | 郑艳萍 Chander Subhash 杨小红 周俊青 李建生 严建兵 | 2006 | 中国农业大学学报2006，11，3： | 7 |
| 4 | Identification of unconditional and conditional QTL for oil, protein and starch content in maize显示文摘Oil, protein and starch are key chemical components of maize kernels. A population of 245 recombinant inbred lines(RILs) derived from a cross between a high-oil inbred line, By804, and a regular inbred line, B73, was used to dissect the genetic interrelationships among oil, starch and protein content at the individual QTL level by unconditional and conditional QTL mapping. Combined phenotypic data over two years with a genetic linkage map constructed using 236 markers, nine, five and eight unconditional QTL were detected for oil, protein and starch content, respectively. Some QTL for oil, protein and starch content were clustered in the same genomic regions and the direction of their effects was consistent with the sign of their correlation. In conditional QTL mapping, 37(29/8) unconditional QTL were not detected or showed reduced effects, four QTL demonstrated similar effects under unconditional and conditional QTL mapping, and 17 additional QTL were identified by conditional QTL mapping. These results imply that there is a strong genetic relationship among oil, protein and starch content in maize kernels. The information generated in the present investigation could be helpful in marker-assisted breeding for maize varieties with desirable kernel quality traits. | Yuqiu Guo Xiaohong Yang Subhash Chander Jianbing Yan Jun Zhang Tongming Song Jiansheng Li | 2013 | The Crop Journal2013，1，1： | 7 |
| 5 | 两个玉米自交系苗期叶温的干旱响应研究显示文摘利用远红外热成像系统的技术,研究玉米自交系综3与87-1苗期叶片对干旱的响应以及生物量的积累变化。结果表明,在遭受中度干旱胁迫的条件下,87-1的相对生物鲜重(0.716)及相对生物干重(0.799)显著高于综3(0.564,0.658),87-1的叶温差(0.30℃)显著高于综3(0.13℃)。通过离体叶片分析,综3离体叶片较87-1失水速率快,叶温在离体短时间内下降更低。表明在响应水分胁迫方面,87-1较综3叶片蒸发量小,叶温变化大,保水能力好,87-1表现出了更强的耐旱性。生物量积累与叶温差存在紧密的相关性,较高叶温差的基因型在遭受到水分胁迫时生物量积累更强。 | 刘亚 丁俊强 Subhash Chander 李建生 | 2008 | 作物杂志2008，，6： | 5 |
| 6 | A genome scan for quantitative trait loci affecting grain yield and its components of maize both in single-and two-locus levels显示文摘By adding thirty-one markers in the pre- vious linkage map, a new genetic linkage map con- taining 205 markers was constructed, spanning a total of 2305.4 cM with an average interval of 11.2 cM. The genotypic errors in the whole genome were de- tected by the statistical method and removed manu- ally. The precision of the linkage map was improved significantly. Main and epistatic QTL were detected by R/qtl, and main QTL were confirmed and refined by multiple interval mapping (MIM). Finally, MIM de- tected seven QTL for rows number, and five QTL for each grain yield, kernels per row and 100-kernel weight. The contribution to genetic variations of QTL varied from 35.3% for grain yield to 61.5% for rows number. Only kernels per row exhibited significant epistatic interactions between QTL. Twenty-four epistatic QTL were detected which distributed on almost all the ten chromosomes. About two-third epistatic QTL were observed between main QTL and another locus, which had no significant effects. These results indicate rather clearly that there are a number of QTL affecting trait expressions, not directly but indirectly through interactions with other loci. Thus, epistatic QTL effects may play a crucial role, if not more important than main QTL effects, in the genetic variation for the measured traits in present study. | YAN Jianbing TANG Hua HUANG Yiqin ZHENG Yonglian SUBHASH Chander LI Jiansheng | 2006 | Chinese Science Bulletin2006，51，12： | 2 |
| 7 | Influence of Rotational Speed on Microstructure and Mechanical Properties of Dissimilar Metal AISI 304-AISI 4140 Continuous Drive Friction Welds显示文摘Fundamental investigation of continuous drive friction welding of austenitic stainless steel (AISI 304) and low alloy steel (AISI 4140) is described. The emphasis is made on the influence of rotational speed on the microstructure and mechanical properties such as hardness, tensile strength, notch tensile strength and impact toughness of the dissimilar joints. Hardness profiles across the weld show the interface is harder than the respective parent metals. In general, maximum peak hardness is observed on the stainless steel side, while other peak hardness is on the low alloy steel side. A trough in hardness distribution in between the peaks is located on the low alloy steel side. Peak hardness on the stainless steel and low alloy steel side close to the interface increases with a decrease in rotational speed. All transverse tensile joints fractured on stainless steel side near the interface. Notch tensile strength and impact toughness increase with increase in rotational speed up to 1 500 r/min and decrease thereafter. The mechanism of influence of rotational speed for the observed trends is discussed in the torque, displacement characteristics, heat generation, microstructure, fractography and mechanical properties. | G Subhash Chander G Madhusudhan Reddy A Venugopal Rao | 2012 | Journal of Iron and Steel Research(International)2012，19，10： | 2 |
| 8 | Real-time adaptive ir- rigation scheduling under a limited water supply显示文摘 | Rao N H Samra PB S Subhash Chander | 1992 | Agrieultur- alWaterManagement1992，，20： | 1 |
| 9 | Life table analysis of the mealybug, Phenacoccus solenopsis Tinsley ( Hemiptera- Pseudococcidae) under laboratory conditions 显示文摘 | Fand BB Gautam RD Chander Subhash S | 2010 | Journal of Entomological Research2010，34，2： | 1 |
| 10 | Removal and recovery of metal ions from acid mine drainage using lignite - A low cost sorbent 显示文摘 | Dinesh Mohan Subhash Chander | 2006 | Journal of Hazardous Materials2006，137，： | 1 |
| 11 | Thickness Dependent Physical Properties of Thermally Evaporated Nanocrystalline CdSe Thin Films显示文摘This paper presents a study on thickness dependent physical properties of cadmium selenide thin films. The films of thickness 445, 631 and 810 nm were deposited employing thermal evaporation technique on glass and ITO-coated glass substrates followed by thermal annealing in air atmosphere at 200 °C. These films were subjected to X-ray diffractometer, UV–Vis spectrophotometer, scanning electron microscopy(SEM) and electrometer for structural, optical,surface morphological and electrical analysis respectively. The structural analysis reveals that the films are nanocrystalline in nature with cubic phase and preferred orientation(111). The crystallographic parameters such as lattice constant, interplanar spacing, grain size, internal strain, dislocation density, number of crystallites per unit area and texture coefficient are calculated and discussed. The optical band gap is found in the range 1.75–1.92 e V and observed to increase with thickness.The SEM study shows that the annealed films are uniform, fully covered and well defined. The electrical analysis shows that the conductivity is varied with film thickness and found within the order of semiconductor behavior. | Anuradha Purohit Subhash Chander Satya Pal Nehra Mahendra Singh Dhaka | 2015 | Acta Metallurgica Sinica(English Letters)2015，28，10： | 1 |
| 12 | Using molecular markers to identify two major loci controlling carotenoid contents in maize grain显示文摘 | Subhash Chander Y. Q. Guo X. H. Yang J. Zhang X. Q. Lu J. B. Yan T. M. Song T. R. Rocheford J. S. Li | 2008 | Theoretical and Applied Genetics2008，，： | 1 |
| 13 | Maize leaf tempera- ture responses to drought: thermal imaging and quantitative trait lo- ci ( QTL ) mapping 显示文摘 | LIU Y CHANDER SUBHASH YAN JB | 2010 | Environmental and Experimental Botany2010，53，： | 1 |
| 14 | Genetic dissection of carotenoids in maize kernels using high-density single nucleotide polymorphism markers in a recombinant inbred line population显示文摘Carotenoids are antioxidants and vitamin A precursors that have important roles in human health. Hence, improving the carotenoid contents in maize kernels is a priority objective for breeders in order to obtain nutritional biofortification outcomes. In the current study, the genetic architecture of carotenoids in maize kernels was explored using a recombinant inbred line(RIL) population derived from a cross between inbred lines By804 and B73. A total of 81 QTLs were detected by using a high-density bin map and a simple sequence repeat(SSR)-based linkage map, with one to seven QTLs for each trait explaining 4.21%–47.53% of the phenotypic variation. A comparison of the QTL mapping efficiency between the two linkage maps revealed that the high-density bin map had higher resolution. In the current study 46 additional QTLs were identified, with 16 being common with previous studies and14 newly identified. Among the results, 29.6%(24/81) of QTLs explained > 10% of the phenotypic variation in the RIL population, and 70.4%(57/81) explained ≤ 10%. These results suggest that a few large-effect QTLs, together with a variable number of minor-effect QTLs,contributed to most of the genetic components of carotenoids in maize kernels. | Orawan Jittham Xiuyi Fu Jing Xu Subhash Chander Jiansheng Li Xiaohong Yang | 2017 | The Crop Journal2017，5，1： | 1 |
| 15 | Neoadjuvant chemotherapy with weekly paclitaxel and carboplatin followed by chemoradiation in locally advanced cervical carcinoma: A pilot study显示文摘 | Rajkumar Bikramjit Singh Subhash Chander B.K. Mohanti Sushmita Pathy Sunesh Kumar Neerja Bhatla Sanjay Thulkar Sreenivas Vishnubhatla Lalit Kumar | 2013 | Gynecologic Oncology2013，，： | 1 |
| 16 | Irrigation scheduling under a limited water supply显示文摘 | Rao NH Sarma PBS Chander Subhash | 1988 | Agricultural Water Management1988，15，： | 1 |
| 17 | 基于基因型选择提高QTL作图的精度——以一个RIL群体为例(英文)显示文摘以PCR为基础的分子标记以及其他检测技术的发展,使得大规模的标记分析成为现实。这也为通过大群体标记分析,然后基于基因型选择挑选合适的小群体,从而提高QTL定位准确性和精度提供了可能。以一个包含294个家系的重组自交系(RIL)群体为例,通过基因型选择和随机选择的办法产生了一系列大小不等的亚群体,比较了两类群体QTL定位的结果。分析表明:相同大小的基因型选择群体与随机群体相比性状的表型分布都符合正态分布;标记的偏分离情况也没有明显的差别,都随着群体大小的增大,偏分离的比例也逐渐增大。但同等大小的基因型选择群体比随机群体的交换富集率(CE)要大,且随着选择强度的增大不断增大,如群体大小为270时,CE=1.04,群体大小为30时,CE=1.45。总体上,随着群体大小的增加,不管是随机群体还是选择群体,其QTL检测能力、灵敏性和特异性也随之增加,但选择群体的检测能力、灵敏性和特异性总体上要好于随机群体。当群体大于或等于240时,其QTL检测能力基本没有差别;群体大小大于或等于210时,其QTL检测的灵敏性和特异性也没有什么差别。这也说明:选择强度越大,效果越明显。以QTL1-LOD区间作为衡量QTL精度的一个指标,结果显示所有基因型选择群体都比相同大小随机群体的QTL定位精度高。目前QTL定位研究中,基因型数据较表型数据而言更容易准确获得,因此通过基因型选择可以更好的优化群体结构,减少田间实验的工作量,提高全基因组水平QTL作图的精度,为随后的QTL辅助选择和精细定位以及克隆提供帮助。 | 严建兵 汤继华 孟义江 马西青 滕文涛 Subhash Chander 李林 李建生 | 2006 | Acta Genetica Sinica2006，33，7： | 1 |
| 18 | Real-time adapting irrigation schedulingunder limited water management显示文摘 | Rao N H Sarma P B S Subhash Chander | 1992 | Agricultural Water Management1992，，20： | 1 |
| 19 | Irrigation scheduling under a limited water supply显示文摘 | Rao N Sarma P Chander Subhash | 1998 | Agricultural Water Management1998，15，2： | 1 |
| 20 | 印度的印刷工业显示文摘全印度印刷商联合会(AIFMP)成立于1953年,由印度政府认可,是印度印刷商的最高机构。全国各地57个协会都是它的会员。AIFMP努力促进南亚区域合作联盟国家中的印刷厂广泛合作,共创未来。 | Subhash Chander | 2009 | 印刷工业2009，，5： | 0 |
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