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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Effects of N ‐acetylcysteine on cytokines in non‐acetaminophen acute liver failure: potential mechanism of improvement in transplant‐free survival显示文摘 | R. Todd Stravitz Arun J. Sanyal Joan Reisch Jasmohan S. Bajaj Farid Mirshahi Jenfeng Cheng William M. Lee | 2013 | Liver Int2013，，9： | 2 |
| 3 | Intravenous N -Acetylcysteine Improves Transplant-Free Survival in Early Stage Non-Acetaminophen Acute Liver Failure显示文摘 | William M. Lee Linda S. Hynan Lorenzo Rossaro Robert J. Fontana R. Todd Stravitz Anne M. Larson Timothy J. Davern Natalie G. Murray Timothy McCashland Joan S. Reisch Patricia R. Robuck | 2009 | Gastroenterology2009，，3： | 2 |
| 4 | Patent brouhaha snares drug firms - Supreme Court examines intellectual property rights used to develop drugs显示文摘 | Reisch M | 2005 | Chemical & Engineering News( S0009 - 2347 )2005，83，17： | 1 |
| 5 | Surgical management of bilateral middle cerebral artery aneurysms via a unilateral supraorbital key-hole craniotomy 显示文摘 | Hopf N J Stadie A Reisch R | 2009 | Minim Invasive Neuro- surg2009，52，3： | 1 |
| 6 | Marker-assisted selection for powdery mildew resistance in grapes显示文摘 | DALBO M A YE G N WEENDEN N F WILCOX W F REISCH B I | 2001 | Journal of the American Society for Horticultural Science2001，126，1： | 1 |
| 7 | Development and application of molecular marker linkage maps in woody fruit crops显示文摘 | N F Weeden M Hemmatt D M Lawson M Lodhi R L Bell A G Manganaris B I Reischs S K Brown G N Ye | 1994 | Euphytica1994，77，12： | 1 |
| 8 | Steroid 17a-hydroxylasedeficiency : Functional characterization of four mutations ( A174E,V178D, R440C, L465P) in the CYP17A1 gene 显示文摘 | Dhir V Reisch N Bleicken CM | 2009 | J Clin EndocrinolMetab2009，94，： | 1 |
| 9 | Minor furocoumarins of Murraya koenigii 显示文摘 | Adebajo A C Reisch J | 2000 | Fitoterapia2000，71，3： | 1 |
| 10 | Optimization of biolistic transformation of embryogenic grape cell suspensions显示文摘 | H(e)bert D Kikkert J R Smith F D Reisch B I | | 0，，： | 1 |
| 11 | Uniportal Endoscopic Surgery of Carpal Tunnel Syndrome: Technique and Clinical Results显示文摘 | R. Filippi R. Reisch D. El Shki P. Grunert | 2002 | Minim Invasive Neurosurg2002，，02： | 1 |
| 12 | Genetic testing for pheochromocytoma-associated syndromes显示文摘 | Pawlu C Bausch B Reisch N | 2005 | Ann Endocrinol (Paris)2005，66，3： | 1 |
| 13 | Image-guided neurosurgery with 3-dimensional multimodal imaging data on a stereoscopic monitor显示文摘 | Kockro RA Reisch R Serra L | 2013 | Neurosurgery2013，721，： | 1 |
| 14 | medical malprac- tice concerns and defensive medicine: a nationwide sur- vey of breast pathologists显示文摘 | Reisch LM Carney PA Oster NV et a/ | 2015 | Am J Clin Pathol2015，144，6： | 1 |
| 15 | Fruit breeding volume Ⅱ 显示文摘 | Pratt C | 1996 | John wiley & Sons Inc1996，334，： | 1 |
| 16 | Modules decomposition of compressional and shear velocity in sand bodies 显示文摘 | MURPHY W REISCHE A HSU K | 1993 | Geophysics1993，58，2： | 1 |
| 17 | Controls on the stable isotope composition of seasonal growth bands in aragonitic freshwater bivalves (Unionidae) 显示文摘 | Dettman D L Reische A L Lohmann K C | 1999 | Geochim Cosmochim Aeta1999，63，78： | 1 |
| 18 | Defining Social Justice in a Socially Unjust World显示文摘 | Reisch Michael | 2002 | Families in Society2002，83，4： | 1 |
| 19 | Virtual reality system for planning minimally invasive neurosurgery显示文摘 | Stadle AT Kockro RA Reisch R | 2008 | J Neurosurg2008，108，2： | 1 |
| 20 | Surgical technique of the supraorbital key-hole craniotomy 显示文摘 | Reisch R Perneczky A Filippi R | 2003 | Surg Neurol2003，59，： | 1 |
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