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| 1 | Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf显示文摘Objective:To evaluate the cytotoxicity and hepatoprotective potentials of extracts,fractions or isolated compound from the leaves of Feronia limonia(F.limonia).Methods:Qualitative phytochemical analysis of extracts,fractions or compound was performed by means of thin layer chromatography and spectroscopic assays.The%purity of compound was measured by analytical HPLC.Extracts,fractions or compound have been individually evaluated for their cytotoxicity effects(10,20,100,250,500,750 and 1 000 μg/mL).Based on the inhibitory concentration(IC_(50)) obtained from the cell viability assay,graded concentrations of extracts,fractions or isolated compound were assessed(10,20,50,100,200 μg/mL) for its hepatoprotective potential against CCl_4-induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase(SGPT) and serum glutamic oxaloacetic transaminase(SGOT).Results:Results indicated that the methanol extract of F.limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract.The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic.However,the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature.Biochemical investigations(SCOT,SCPT) further corroborated these cytological observations.Conclusions:It can be concluded from this study that F.limonia methanol extract,some fractions and pure isolated compound herein exhibit hepatoprotective activity.However,cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study. | Mahendra Jain Rakhee Kapadia Ravirajsinh N Jadeja Menaka C Thounaojam Ranjitsinh V Devkar SH Mishra | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，6： | 11 |
| 2 | N-policy for a machine repair system with spares and reneging显示文摘 | JAIN M Rakhee MAHESHWARI S | 2004 | Applied Mathematical Modeling2004，28，： | 1 |
| 3 | Diagnostic yield of EUS-guided FNA and cytology in suspected tubercular intra-abdominal lymphadenopathy显示文摘 | Rajesh Puri Rakhee Mangla Mohamad Eloubeidi Peter Vilmann Ragesh Thandassery Randhir Sud | 2012 | Gastrointestinal Endoscopy2012，，5： | 1 |
| 4 | Role of diffusion- weighted imping in differentiating benign and malignant ped- iatric abdominal tumors 显示文摘 | Rakhee SG Gabriel G SOlomon M | 2013 | Pediatr Radiol2013，43，7： | 1 |
| 5 | Ionising radiation-free whole-body MRI versus 18 F-fluorodeoxyglucose PET/CT scans for children and young adults with cancer: a prospective, non-randomised, single-centre study显示文摘 | Christopher Klenk Rakhee Gawande Lebriz Uslu Aman Khurana Deqiang Qiu Andrew Quon Jessica Donig Jarrett Rosenberg Sandra Luna-Fineman Michael Moseley Heike E Daldrup-Link | 2014 | Lancet Oncology2014，，： | 1 |
| 6 | Bilevel control of degraded machining system with warm standbys, setup and vacation显示文摘 | Jain M Rakhee Mahavir S | 2004 | Applied Mathematical Modelling2004，28，： | 1 |
| 7 | Bilevel control of degraded machining system with warm standbys,setup and vacation显示文摘 | Jain M Rakhee Singh M | | 0，，12： | 1 |
| 8 | Building a Sensor Network with PSoC显示文摘 | Rakhee Mohiddin Manoj Kumar Shashi Kumar Palakurty | 2011 | IEEE2011，，5： | 1 |
| 9 | 人DNA聚合酶δ结合蛋白PDIP38的克隆、表达和纯化显示文摘[目的]克隆表达和纯化野生型及3种缺失突变型人PDIP38谷胱甘肽S转移酶(glutathion S trans-ferase,GST)融合蛋白。[方法]采用常规PCR扩增法得到野生型及3种缺失突变型人PDIP38的cDNA;4种cD-NA与GST融合载体pGEX-4T-1重组并转化大肠杆菌DH5α;采用BamH Ⅰ和Xho Ⅰ双酶切鉴定插入的DNA序列;采用谷胱甘肽-sepharose 4B亲和层析柱纯化重组蛋白。[结果]野生型和3种缺失突变型人PDIP38 GST融合蛋白在DH5α中高效表达;经谷胱甘肽-sepharose 4B亲和层析柱一步纯化后,4种蛋白的纯度均达到85%以上。[结论]pGEX-4T-1 GST蛋白融合载体可高效表达人PDIP38蛋白;用谷胱甘肽-sepharose 4B亲和层析柱一步纯化法可简单、快速地得到高纯度的蛋白。 | 翁云 Rakhee Gupte Marietta Y.W.T.Lee 梁念慈 | 2004 | 中山大学学报(医学科学版)2004，25，1： | 1 |
| 10 | N-policy for a machine repair system with spares and reneging显示文摘 | Jain M Rakhee Maheshwari S | 2004 | Applied Mathematical Modelling2004，28，6： | 1 |
| 11 | Bilevel control of degraded machining system with warm standbys setup and vacation显示文摘 | Jain M Rakhee Singh M | 2004 | Applied Mathematical Modelling2004，28，12： | 1 |
| 12 | Time efficient indexing technique for querying location dependent data in wireless broadcast显示文摘 | VERMA S KUMARI S RAKHEE | 2010 | International Journal of Computer Application2010，7，10： | 1 |
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